Restriction map for
pF1KB8582
(Circular) (Six-base) MAPSORT of: pF1KB8582.seq Check: 3777 from: 1 to: 5360
pF1KB8582 5360 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1277 1803 1277
Size: 526 4834
AccI GT'mk_AC
Cuts at: 1403 1565 2398 1403
Size: 162 833 4365
Fragments arranged by size:
4365 833 162
Acc65I G'GTAC_C
Cuts at: 2376 2376
Size: 5360
AclI AA'CG_TT
Cuts at: 5200 5200
Size: 5360
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 355 381 547 1645 1789 2147 3185 3617 3970
Size: 26 166 1098 144 358 1038 432 353
Cuts at: 3970 4632 355
Size: 662 1083
Fragments arranged by size:
1098 1083 1038 662 432 358 353 166
144 26
AflIII A'CryG_T
Cuts at: 2712 4512 4793 2712
Size: 1800 281 3279
Fragments arranged by size:
3279 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 186 186
Size: 5360
AleI CACnn'nnGTG
Cuts at: 1126 1126
Size: 5360
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 823 826 1875 823
Size: 3 1049 4308
Fragments arranged by size:
4308 1049 3
AlwNI CAG_nnn'CTG
Cuts at: 387 771 1067 1824 1970 2301 2665 4103 387
Size: 384 296 757 146 331 364 1438 1644
Fragments arranged by size:
1644 1438 757 384 364 331 296 146
ApaI G_GGCC'C
Cuts at: 1378 1378
Size: 5360
ApaLI G'TGCA_C
Cuts at: 1333 4198 1333
Size: 2865 2495
ApoI r'AATT_y
Cuts at: 207 540 691 2364 4713 4969 207
Size: 333 151 1673 2349 256 598
Fragments arranged by size:
2349 1673 598 333 256 151
AseI AT'TA_AT
Cuts at: 20 20
Size: 5360
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5360
AvaI C'yCGr_G
Cuts at: 349 659 1503 1512 1931 2380 349
Size: 310 844 9 419 449 3329
Fragments arranged by size:
3329 844 449 419 310 9
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 619 652 619
Size: 33 5327
BamHI G'GATC_C
Cuts at: 2385 2385
Size: 5360
BanI G'GyrC_C
Cuts at: 134 948 1156 1352 2376 2790 3038 3073 134
Size: 814 208 196 1024 414 248 35 2421
Fragments arranged by size:
2421 1024 814 414 248 208 196 35
BanII G_rGCy'C
Cuts at: 277 874 1378 1513 1597 2374 3404 277
Size: 597 504 135 84 777 1030 2233
Fragments arranged by size:
2233 1030 777 597 504 135 84
BbeI G_GCGC'C
Cuts at: 138 2794 3042 138
Size: 2656 248 2456
Fragments arranged by size:
2656 2456 248
BbsI GAAGACnn'nnnn_
Cuts at: 2036 2204 2036
Size: 168 5192
BbvCI CC'TCA_GC
Cuts at: 680 1057 680
Size: 377 4983
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1460 1494 1460
Size: 34 5326
BciVI GTATCCnnnnn_n'
Cuts at: 2156 3258 4314 5360 2156
Size: 1102 1056 1046 2156
Fragments arranged by size:
2156 1102 1056 1046
BclI T'GATC_A
Cuts at: 2880 2880
Size: 5360
BglII A'GATC_T
Cuts at: 2875 4912 2875
Size: 2037 3323
BlpI GC'TnA_GC
Cuts at: 2472 2472
Size: 5360
Bme1580I G_kGCm'C
Cuts at: 953 1161 1337 1378 1491 2985 3078 4202 953
Size: 208 176 41 113 1494 93 1124 2111
Fragments arranged by size:
2111 1494 1124 208 176 113 93 41
BmrI ACTGGGnnnn_n'
Cuts at: 1028 2572 2653 2988 5132 1028
Size: 1544 81 335 2144 1256
Fragments arranged by size:
2144 1544 1256 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1164 2166 3727 1164
Size: 1002 1561 2797
Fragments arranged by size:
2797 1561 1002
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 478 748 1231 1375 1567 1720 2023 2347 478
Size: 270 483 144 192 153 303 324 3491
Fragments arranged by size:
3491 483 324 303 270 192 153 144
Bpu10I CC'TnA_GC
Cuts at: 680 1057 1363 2176 680
Size: 377 306 813 3864
Fragments arranged by size:
3864 813 377 306
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 976 1090 2536 3415 4129 4427 976
Size: 114 1446 879 714 298 1909
Fragments arranged by size:
1909 1446 879 714 298 114
BsaI GGTCTCn'nnnn_
Cuts at: 338 740 2249 5064 338
Size: 402 1509 2815 634
Fragments arranged by size:
2815 1509 634 402
BsaAI yAC'GTr
Cuts at: 1124 2713 1124
Size: 1589 3771
BsaBI GATnn'nnATC
Cuts at: 6 2874 4911 6
Size: 2868 2037 455
Fragments arranged by size:
2868 2037 455
BsaHI Gr'CG_yC
Cuts at: 135 1889 2791 3039 3741 5237 135
Size: 1754 902 248 702 1496 258
Fragments arranged by size:
1754 1496 902 702 258 248
BsaWI w'CCGG_w
Cuts at: 255 2549 3070 3748 4159 4306 4580 255
Size: 2294 521 678 411 147 274 1035
Fragments arranged by size:
2294 1035 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1642 1669 1672 1699 1642
Size: 27 3 27 5303
Fragments arranged by size:
5303 27 27 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 433 487 490 1039 2230 2233 2242 433
Size: 54 3 549 1191 3 9 3551
Fragments arranged by size:
3551 1191 549 54 9 3 3
BseYI C'CCAG_C
Cuts at: 1378 2785 3543 4208 4616 1378
Size: 1407 758 665 408 2122
Fragments arranged by size:
2122 1407 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1693 1837 1693
Size: 144 5216
BsiEI CG_ry'CG
Cuts at: 104 923 2902 2948 4178 104
Size: 819 1979 46 1230 1286
Fragments arranged by size:
1979 1286 1230 819 46
BsiHKAI G_wGCw'C
Cuts at: 314 1018 1337 1369 1513 1597 2182 2374 3152
Size: 704 319 32 144 84 585 192 778
Cuts at: 3152 3342 4202 314
Size: 190 860 1472
Fragments arranged by size:
1472 860 778 704 585 319 192 190
144 84 32
BsmI GAATG_Cn'
Cuts at: 715 1478 715
Size: 763 4597
BsmBI CGTCTCn'nnnn_
Cuts at: 898 1896 898
Size: 998 4362
Bsp1286I G_dGCh'C
Cuts at: 277 314 874 953 1018 1161 1337 1369 1378
Size: 37 560 79 65 143 176 32 9
Cuts at: 1378 1491 1513 1597 2182 2374 2985 3078 3152
Size: 113 22 84 585 192 611 93 74
Cuts at: 3152 3342 3404 4202 277
Size: 190 62 798 1435
Fragments arranged by size:
1435 798 611 585 560 192 190 176
143 113 93 84 79 74 65 62
37 32 22 9
BspEI T'CCGG_A
Cuts at: 255 255
Size: 5360
BspHI T'CATG_A
Cuts at: 3792 3792
Size: 5360
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1277 1551 1652 1715 1803 2410 2624 2926 3307
Size: 274 101 63 88 607 214 302 381
Cuts at: 3307 1277
Size: 3330
Fragments arranged by size:
3330 607 381 302 274 214 101 88
63
BsrBI CCG'CTC
Cuts at: 50 487 831 2022 3652 3706 5190 5355 50
Size: 437 344 1191 1630 54 1484 165 55
Fragments arranged by size:
1630 1484 1191 437 344 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3272 3272
Size: 5360
BsrFI r'CCGG_y
Cuts at: 287 1070 1808 3358 3539 287
Size: 783 738 1550 181 2108
Fragments arranged by size:
2108 1550 783 738 181
BsrGI T'GTAC_A
Cuts at: 1115 1316 1115
Size: 201 5159
BssSI C'ACGA_G
Cuts at: 3631 4339 3631
Size: 708 4652
BstAPI GCAn_nnn'nTGC
Cuts at: 721 1067 1332 1824 2737 721
Size: 346 265 492 913 3344
Fragments arranged by size:
3344 913 492 346 265
BstBI TT'CG_AA
Cuts at: 3721 3721
Size: 5360
BstEII G'GTnAC_C
Cuts at: 729 729
Size: 5360
BstXI CCAn_nnnn'nTGG
Cuts at: 885 885
Size: 5360
BstYI r'GATC_y
Cuts at: 1 1994 2331 2385 2852 2875 3210 3456 3762
Size: 1993 337 54 467 23 335 246 306
Cuts at: 3762 3774 3860 4912 1
Size: 12 86 1052 449
Fragments arranged by size:
1993 1052 467 449 337 335 306 246
86 54 23 12
BtgI C'CryG_G
Cuts at: 107 563 107
Size: 456 4904
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3310 3491 3654 3310
Size: 181 163 5016
Fragments arranged by size:
5016 181 163
BtsI GCAGTG_nn'
Cuts at: 982 1189 1335 1367 1448 1476 1722 1913 2603
Size: 207 146 32 81 28 246 191 690
Cuts at: 2603 982
Size: 3739
Fragments arranged by size:
3739 690 246 207 191 146 81 32
28
DraI TTT'AAA
Cuts at: 2100 2359 2100
Size: 259 5101
DrdI GACnn_nn'nnGTC
Cuts at: 3066 4410 3066
Size: 1344 4016
EaeI y'GGCC_r
Cuts at: 281 650 875 920 1796 2945 3119 3510 3537
Size: 369 225 45 876 1149 174 391 27
Cuts at: 3537 4882 281
Size: 1345 759
Fragments arranged by size:
1345 1149 876 759 391 369 225 174
45 27
EagI C'GGCC_G
Cuts at: 920 2945 920
Size: 2025 3335
EarI CTCTTCn'nnn_
Cuts at: 419 1348 1931 2123 2201 3383 3593 419
Size: 929 583 192 78 1182 210 2186
Fragments arranged by size:
2186 1182 929 583 210 192 78
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 157 1240 3568 4310 4456 5170 157
Size: 1083 2328 742 146 714 347
Fragments arranged by size:
2328 1083 742 714 347 146
EcoICRI GAG'CTC
Cuts at: 1511 1595 2372 1511
Size: 84 777 4499
Fragments arranged by size:
4499 777 84
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 355 381 478 547 748 1231 1375 1567 1645
Size: 26 97 69 201 483 144 192 78
Cuts at: 1645 1720 1789 2023 2147 2347 3185 3617 3970
Size: 75 69 234 124 200 838 432 353
Cuts at: 3970 4632 355
Size: 662 1083
Fragments arranged by size:
1083 838 662 483 432 353 234 201
200 192 144 124 97 78 75 69
69 26
EcoO109I rG'GnC_Cy
Cuts at: 1359 1375 1517 2171 2499 1359
Size: 16 142 654 328 4220
Fragments arranged by size:
4220 654 328 142 16
EcoRI G'AATT_C
Cuts at: 540 2364 540
Size: 1824 3536
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3379 3379
Size: 5360
FspI TGC'GCA
Cuts at: 1273 3141 1273
Size: 1868 3492
HaeII r_GCGC'y
Cuts at: 138 2794 3042 4272 5043 138
Size: 2656 248 1230 771 455
Fragments arranged by size:
2656 1230 771 455 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 172 178 204 210 310 342 3202 3232 3234
Size: 6 26 6 100 32 2860 30 2
Cuts at: 3234 3264 172
Size: 30 2268
Fragments arranged by size:
2860 2268 100 32 30 30 26 6
6 2
HincII GTy'rAC
Cuts at: 1566 1641 2399 1566
Size: 75 758 4527
Fragments arranged by size:
4527 758 75
HindIII A'AGCT_T
Cuts at: 841 841
Size: 5360
KasI G'GCGC_C
Cuts at: 134 2790 3038 134
Size: 2656 248 2456
Fragments arranged by size:
2656 2456 248
KpnI G_GTAC'C
Cuts at: 2380 2380
Size: 5360
MluI A'CGCG_T
Cuts at: 4793 4793
Size: 5360
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 881 4119 4303 881
Size: 3238 184 1938
Fragments arranged by size:
3238 1938 184
MscI TGG'CCA
Cuts at: 652 1798 3121 652
Size: 1146 1323 2891
Fragments arranged by size:
2891 1323 1146
MslI CAynn'nnrTG
Cuts at: 940 1126 1528 3349 3476 940
Size: 186 402 1821 127 2824
Fragments arranged by size:
2824 1821 402 186 127
MspA1I CmG'CkG
Cuts at: 115 646 885 1467 1683 1767 1824 2001 2301
Size: 531 239 582 216 84 57 177 300
Cuts at: 2301 2471 2785 3145 3929 4174 115
Size: 170 314 360 784 245 1301
Fragments arranged by size:
1301 784 582 531 360 314 300 245
239 216 177 170 84 57
NaeI GCC'GGC
Cuts at: 289 1072 1810 3541 289
Size: 783 738 1731 2108
Fragments arranged by size:
2108 1731 783 738
NarI GG'CG_CC
Cuts at: 135 2791 3039 135
Size: 2656 248 2456
Fragments arranged by size:
2656 2456 248
NcoI C'CATG_G
Cuts at: 107 563 107
Size: 456 4904
NgoMIV G'CCGG_C
Cuts at: 287 1070 1808 3539 287
Size: 783 738 1731 2108
Fragments arranged by size:
2108 1731 783 738
NspI r_CATG'y
Cuts at: 987 2413 2913 4516 987
Size: 1426 500 1603 1831
Fragments arranged by size:
1831 1603 1426 500
PasI CC'CwG_GG
Cuts at: 2284 2307 2284
Size: 23 5337
PciI A'CATG_T
Cuts at: 4512 4512
Size: 5360
PflMI CCAn_nnn'nTGG
Cuts at: 624 1398 624
Size: 774 4586
PfoI T'CCnGG_A
Cuts at: 1544 1736 1781 1544
Size: 192 45 5123
Fragments arranged by size:
5123 192 45
PmeI GTTT'AAAC
Cuts at: 2359 2359
Size: 5360
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 418 450 3806 3838 418
Size: 32 3356 32 1940
Fragments arranged by size:
3356 1940 32 32
PpuMI rG'GwC_Cy
Cuts at: 1359 1359
Size: 5360
PspOMI G'GGCC_C
Cuts at: 1374 1374
Size: 5360
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1837 1869 1837
Size: 32 5328
PstI C_TGCA'G
Cuts at: 371 815 1649 1766 1864 2297 2339 2407 3092
Size: 444 834 117 98 433 42 68 685
Cuts at: 3092 371
Size: 2639
Fragments arranged by size:
2639 834 685 444 433 117 98 68
42
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5360
PvuII CAG'CTG
Cuts at: 115 646 885 1683 1767 1824 2001 2301 2785
Size: 531 239 798 84 57 177 300 484
Cuts at: 2785 3145 115
Size: 360 2330
Fragments arranged by size:
2330 798 531 484 360 300 239 177
84 57
RsrII CG'GwC_CG
Cuts at: 3555 3555
Size: 5360
SacI G_AGCT'C
Cuts at: 1513 1597 2374 1513
Size: 84 777 4499
Fragments arranged by size:
4499 777 84
SalI G'TCGA_C
Cuts at: 1564 2397 1564
Size: 833 4527
SapI GCTCTTCn'nnn_
Cuts at: 3383 3593 3383
Size: 210 5150
SbfI CC_TGCA'GG
Cuts at: 371 1649 2407 371
Size: 1278 758 3324
Fragments arranged by size:
3324 1278 758
ScaI AGT'ACT
Cuts at: 4877 4877
Size: 5360
SfcI C'TryA_G
Cuts at: 33 367 811 1645 1762 1860 1962 2293 2335
Size: 334 444 834 117 98 102 331 42
Cuts at: 2335 2403 3088 4056 4247 33
Size: 68 685 968 191 1146
Fragments arranged by size:
1146 968 834 685 444 334 331 191
117 102 98 68 42
SfoI GGC'GCC
Cuts at: 136 2792 3040 136
Size: 2656 248 2456
Fragments arranged by size:
2656 2456 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5360
SmaI CCC'GGG
Cuts at: 1933 2382 1933
Size: 449 4911
SmlI C'TyrA_G
Cuts at: 991 1105 2515 3430 4144 4406 991
Size: 114 1410 915 714 262 1945
Fragments arranged by size:
1945 1410 915 714 262 114
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5360
SphI G_CATG'C
Cuts at: 987 2413 2913 987
Size: 1426 500 3434
Fragments arranged by size:
3434 1426 500
StyI C'CwwG_G
Cuts at: 107 563 1092 1397 2494 2848 107
Size: 456 529 305 1097 354 2619
Fragments arranged by size:
2619 1097 529 456 354 305
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2943 3076 3535 3744 2943
Size: 133 459 209 4559
Fragments arranged by size:
4559 459 209 133
TatI w'GTAC_w
Cuts at: 1115 1316 1958 4875 1115
Size: 201 642 2917 1600
Fragments arranged by size:
2917 1600 642 201
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 831 863 4877 4909 831
Size: 32 4014 32 1282
Fragments arranged by size:
4014 1282 32 32
Tth111I GACn'n_nGTC
Cuts at: 511 3157 511
Size: 2646 2714
XbaI T'CTAG_A
Cuts at: 2391 2391
Size: 5360
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1423 2855 1423
Size: 1432 3928
XmaI C'CCGG_G
Cuts at: 1931 2380 1931
Size: 449 4911
XmnI GAAnn'nnTTC
Cuts at: 1774 1774
Size: 5360
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AhdI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PspOMI PsrI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AloI AscI AvrII BfrBI
BglI BmgBI BmtI BsiWI BssHII BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PmlI PshAI
PsiI PspXI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI ZraI