Restriction map for
pF1KB8586
(Circular) (Six-base) MAPSORT of: pF1KB8586.seq Check: 5870 from: 1 to: 4031
pF1KB8586 4031 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AccI GT'mk_AC
Cuts at: 1069 1069
Size: 4031
Acc65I G'GTAC_C
Cuts at: 1047 1047
Size: 4031
AclI AA'CG_TT
Cuts at: 3871 3871
Size: 4031
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1856 2288 2641 3303 1856
Size: 432 353 662 2584
Fragments arranged by size:
2584 662 432 353
AflIII A'CryG_T
Cuts at: 873 1383 3183 3464 873
Size: 510 1800 281 1440
Fragments arranged by size:
1800 1440 510 281
AleI CACnn'nnGTG
Cuts at: 892 892
Size: 4031
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 673 705 673
Size: 32 3999
AlwNI CAG_nnn'CTG
Cuts at: 1009 1336 2774 1009
Size: 327 1438 2266
Fragments arranged by size:
2266 1438 327
ApaLI G'TGCA_C
Cuts at: 2869 2869
Size: 4031
ApoI r'AATT_y
Cuts at: 139 577 1035 3384 3640 139
Size: 438 458 2349 256 530
Fragments arranged by size:
2349 530 458 438 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4031
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4031
AvaI C'yCGr_G
Cuts at: 421 1051 421
Size: 630 3401
BamHI G'GATC_C
Cuts at: 201 232 1056 201
Size: 31 824 3176
Fragments arranged by size:
3176 824 31
BanI G'GyrC_C
Cuts at: 1047 1461 1709 1744 1047
Size: 414 248 35 3334
Fragments arranged by size:
3334 414 248 35
BanII G_rGCy'C
Cuts at: 1045 2075 1045
Size: 1030 3001
BbeI G_GCGC'C
Cuts at: 1465 1713 1465
Size: 248 3783
BbsI GAAGACnn'nnnn_
Cuts at: 562 562
Size: 4031
BciVI GTATCCnnnnn_n'
Cuts at: 1929 2985 4031 1929
Size: 1056 1046 1929
Fragments arranged by size:
1929 1056 1046
BclI T'GATC_A
Cuts at: 1551 1551
Size: 4031
BglII A'GATC_T
Cuts at: 462 1546 3583 462
Size: 1084 2037 910
Fragments arranged by size:
2037 1084 910
BlpI GC'TnA_GC
Cuts at: 1143 1143
Size: 4031
Bme1580I G_kGCm'C
Cuts at: 575 1656 1749 2873 575
Size: 1081 93 1124 1733
Fragments arranged by size:
1733 1124 1081 93
BmrI ACTGGGnnnn_n'
Cuts at: 283 1243 1324 1659 3803 283
Size: 960 81 335 2144 511
Fragments arranged by size:
2144 960 511 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2398 2398
Size: 4031
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 724 724
Size: 4031
Bpu10I CC'TnA_GC
Cuts at: 246 246
Size: 4031
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1207 2086 2800 3098 1207
Size: 879 714 298 2140
Fragments arranged by size:
2140 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 3735 3735
Size: 4031
BsaAI yAC'GTr
Cuts at: 1384 1384
Size: 4031
BsaBI GATnn'nnATC
Cuts at: 6 1545 3582 6
Size: 1539 2037 455
Fragments arranged by size:
2037 1539 455
BsaHI Gr'CG_yC
Cuts at: 1462 1710 2412 3908 1462
Size: 248 702 1496 1585
Fragments arranged by size:
1585 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1220 1741 2419 2830 2977 3251 1220
Size: 521 678 411 147 274 2000
Fragments arranged by size:
2000 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 673 697 703 727 975 1005 673
Size: 24 6 24 248 30 3699
Fragments arranged by size:
3699 248 30 24 24 6
BseYI C'CCAG_C
Cuts at: 1456 2214 2879 3287 1456
Size: 758 665 408 2200
Fragments arranged by size:
2200 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 1573 1619 2849 104
Size: 1469 46 1230 1286
Fragments arranged by size:
1469 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1045 1823 2013 2873 1045
Size: 778 190 860 2203
Fragments arranged by size:
2203 860 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 693 693
Size: 4031
Bsp1286I G_dGCh'C
Cuts at: 575 1045 1656 1749 1823 2013 2075 2873 575
Size: 470 611 93 74 190 62 798 1733
Fragments arranged by size:
1733 798 611 470 190 93 74 62
BspHI T'CATG_A
Cuts at: 800 2463 800
Size: 1663 2368
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1081 1295 1597 1978 1081
Size: 214 302 381 3134
Fragments arranged by size:
3134 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2323 2377 3861 4026 50
Size: 2273 54 1484 165 55
Fragments arranged by size:
2273 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 153 842 1943 153
Size: 689 1101 2241
Fragments arranged by size:
2241 1101 689
BsrFI r'CCGG_y
Cuts at: 2029 2210 2029
Size: 181 3850
BssSI C'ACGA_G
Cuts at: 2302 3010 2302
Size: 708 3323
BstAPI GCAn_nnn'nTGC
Cuts at: 1408 1408
Size: 4031
BstBI TT'CG_AA
Cuts at: 2392 2392
Size: 4031
BstYI r'GATC_y
Cuts at: 1 201 232 403 462 823 1056 1523 1546
Size: 200 31 171 59 361 233 467 23
Cuts at: 1546 1881 2127 2433 2445 2531 3583 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
1052 467 449 361 335 306 246 233
200 171 86 59 31 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4031
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1981 2162 2325 1981
Size: 181 163 3687
Fragments arranged by size:
3687 181 163
BtsI GCAGTG_nn'
Cuts at: 1274 1274
Size: 4031
DraI TTT'AAA
Cuts at: 1030 1030
Size: 4031
DrdI GACnn_nn'nnGTC
Cuts at: 1737 3081 1737
Size: 1344 2687
EaeI y'GGCC_r
Cuts at: 847 884 1616 1790 2181 2208 3553 847
Size: 37 732 174 391 27 1345 1325
Fragments arranged by size:
1345 1325 732 391 174 37 27
EagI C'GGCC_G
Cuts at: 1616 1616
Size: 4031
EarI CTCTTCn'nnn_
Cuts at: 246 739 2054 2264 246
Size: 493 1315 210 2013
Fragments arranged by size:
2013 1315 493 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2239 2981 3127 3841 2239
Size: 742 146 714 2429
Fragments arranged by size:
2429 742 714 146
EcoICRI GAG'CTC
Cuts at: 1043 1043
Size: 4031
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 724 1856 2288 2641 3303 724
Size: 1132 432 353 662 1452
Fragments arranged by size:
1452 1132 662 432 353
EcoO109I rG'GnC_Cy
Cuts at: 1170 1170
Size: 4031
EcoRI G'AATT_C
Cuts at: 577 1035 577
Size: 458 3573
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 424 2050 424
Size: 1626 2405
FspI TGC'GCA
Cuts at: 1812 1812
Size: 4031
HaeII r_GCGC'y
Cuts at: 1465 1713 2943 3714 1465
Size: 248 1230 771 1782
Fragments arranged by size:
1782 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 716 748 954 986 1873 1903 1905 1935 716
Size: 32 206 32 887 30 2 30 2812
Fragments arranged by size:
2812 887 206 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1070 1070
Size: 4031
KasI G'GCGC_C
Cuts at: 1461 1709 1461
Size: 248 3783
KpnI G_GTAC'C
Cuts at: 1051 1051
Size: 4031
MfeI C'AATT_G
Cuts at: 890 899 890
Size: 9 4022
MluI A'CGCG_T
Cuts at: 873 3464 873
Size: 2591 1440
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 554 769 2790 2974 554
Size: 215 2021 184 1611
Fragments arranged by size:
2021 1611 215 184
MscI TGG'CCA
Cuts at: 849 886 1792 849
Size: 37 906 3088
Fragments arranged by size:
3088 906 37
MslI CAynn'nnrTG
Cuts at: 892 2020 2147 892
Size: 1128 127 2776
Fragments arranged by size:
2776 1128 127
MspA1I CmG'CkG
Cuts at: 853 1142 1456 1816 2600 2845 853
Size: 289 314 360 784 245 2039
Fragments arranged by size:
2039 784 360 314 289 245
NaeI GCC'GGC
Cuts at: 2212 2212
Size: 4031
NarI GG'CG_CC
Cuts at: 1462 1710 1462
Size: 248 3783
NcoI C'CATG_G
Cuts at: 107 107
Size: 4031
NdeI CA'TA_TG
Cuts at: 914 914
Size: 4031
NgoMIV G'CCGG_C
Cuts at: 2210 2210
Size: 4031
NspI r_CATG'y
Cuts at: 1084 1584 3187 1084
Size: 500 1603 1928
Fragments arranged by size:
1928 1603 500
PasI CC'CwG_GG
Cuts at: 810 810
Size: 4031
PciI A'CATG_T
Cuts at: 3183 3183
Size: 4031
PmeI GTTT'AAAC
Cuts at: 1030 1030
Size: 4031
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 673 705 2477 2509 673
Size: 32 1772 32 2195
Fragments arranged by size:
2195 1772 32 32
PstI C_TGCA'G
Cuts at: 1014 1078 1763 1014
Size: 64 685 3282
Fragments arranged by size:
3282 685 64
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4031
PvuII CAG'CTG
Cuts at: 853 1456 1816 853
Size: 603 360 3068
Fragments arranged by size:
3068 603 360
RsrII CG'GwC_CG
Cuts at: 2226 2226
Size: 4031
SacI G_AGCT'C
Cuts at: 1045 1045
Size: 4031
SalI G'TCGA_C
Cuts at: 1068 1068
Size: 4031
SapI GCTCTTCn'nnn_
Cuts at: 2054 2264 2054
Size: 210 3821
SbfI CC_TGCA'GG
Cuts at: 1078 1078
Size: 4031
ScaI AGT'ACT
Cuts at: 3548 3548
Size: 4031
SexAI A'CCwGG_T
Cuts at: 380 380
Size: 4031
SfcI C'TryA_G
Cuts at: 33 1010 1074 1759 2727 2918 33
Size: 977 64 685 968 191 1146
Fragments arranged by size:
1146 977 968 685 191 64
SfoI GGC'GCC
Cuts at: 1463 1711 1463
Size: 248 3783
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4031
SmaI CCC'GGG
Cuts at: 423 1053 423
Size: 630 3401
SmlI C'TyrA_G
Cuts at: 1186 2101 2815 3077 1186
Size: 915 714 262 2140
Fragments arranged by size:
2140 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4031
SphI G_CATG'C
Cuts at: 1084 1584 1084
Size: 500 3531
StyI C'CwwG_G
Cuts at: 107 1165 1519 107
Size: 1058 354 2619
Fragments arranged by size:
2619 1058 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1614 1747 2206 2415 1614
Size: 133 459 209 3230
Fragments arranged by size:
3230 459 209 133
TatI w'GTAC_w
Cuts at: 3546 3546
Size: 4031
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 697 729 3548 3580 697
Size: 32 2819 32 1148
Fragments arranged by size:
2819 1148 32 32
Tth111I GACn'n_nGTC
Cuts at: 1828 1828
Size: 4031
XbaI T'CTAG_A
Cuts at: 1062 1062
Size: 4031
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1526 1526
Size: 4031
XmaI C'CCGG_G
Cuts at: 421 1051 421
Size: 630 3401
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AleI AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NspI
PasI PciI PmeI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AlfI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI BseRI BsgI BsiWI BsmI BspEI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI NheI NotI NruI
NsiI PacI PflMI PfoI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI