Restriction map for
pF1KB8587
(Circular) (Six-base) MAPSORT of: pF1KB8587.seq Check: 8437 from: 1 to: 3824
pF1KB8587 3824 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AccI GT'mk_AC
Cuts at: 862 862
Size: 3824
Acc65I G'GTAC_C
Cuts at: 840 840
Size: 3824
AclI AA'CG_TT
Cuts at: 3664 3664
Size: 3824
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 356 712 1649 2081 2434 3096 356
Size: 356 937 432 353 662 1084
Fragments arranged by size:
1084 937 662 432 356 353
AflIII A'CryG_T
Cuts at: 1176 2976 3257 1176
Size: 1800 281 1743
Fragments arranged by size:
1800 1743 281
AhdI GACnn_n'nnGTC
Cuts at: 384 384
Size: 3824
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 521 553 521
Size: 32 3792
AlwNI CAG_nnn'CTG
Cuts at: 641 1129 2567 641
Size: 488 1438 1898
Fragments arranged by size:
1898 1438 488
ApaI G_GGCC'C
Cuts at: 177 177
Size: 3824
ApaLI G'TGCA_C
Cuts at: 2662 2662
Size: 3824
ApoI r'AATT_y
Cuts at: 475 531 661 828 3177 3433 475
Size: 56 130 167 2349 256 866
Fragments arranged by size:
2349 866 256 167 130 56
AseI AT'TA_AT
Cuts at: 20 20
Size: 3824
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3824
AvaI C'yCGr_G
Cuts at: 176 844 176
Size: 668 3156
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 628 661 628
Size: 33 3791
BamHI G'GATC_C
Cuts at: 849 849
Size: 3824
BanI G'GyrC_C
Cuts at: 840 1254 1502 1537 840
Size: 414 248 35 3127
Fragments arranged by size:
3127 414 248 35
BanII G_rGCy'C
Cuts at: 130 177 515 838 1868 130
Size: 47 338 323 1030 2086
Fragments arranged by size:
2086 1030 338 323 47
BbeI G_GCGC'C
Cuts at: 1258 1506 1258
Size: 248 3576
BbsI GAAGACnn'nnnn_
Cuts at: 814 814
Size: 3824
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3790
BciVI GTATCCnnnnn_n'
Cuts at: 656 1722 2778 3824 656
Size: 1066 1056 1046 656
Fragments arranged by size:
1066 1056 1046 656
BclI T'GATC_A
Cuts at: 1344 1344
Size: 3824
BglI GCCn_nnn'nGGC
Cuts at: 208 208
Size: 3824
BglII A'GATC_T
Cuts at: 1339 3376 1339
Size: 2037 1787
BlpI GC'TnA_GC
Cuts at: 227 936 227
Size: 709 3115
Bme1580I G_kGCm'C
Cuts at: 177 696 1449 1542 2666 177
Size: 519 753 93 1124 1335
Fragments arranged by size:
1335 1124 753 519 93
BmrI ACTGGGnnnn_n'
Cuts at: 570 1036 1117 1452 3596 570
Size: 466 81 335 2144 798
Fragments arranged by size:
2144 798 466 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 242 2191 242
Size: 1949 1875
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 114 271 491 114
Size: 157 220 3447
Fragments arranged by size:
3447 220 157
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1000 1879 2593 2891 1000
Size: 879 714 298 1933
Fragments arranged by size:
1933 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 3528 3528
Size: 3824
BsaAI yAC'GTr
Cuts at: 1177 1177
Size: 3824
BsaBI GATnn'nnATC
Cuts at: 6 1338 3375 6
Size: 1332 2037 455
Fragments arranged by size:
2037 1332 455
BsaHI Gr'CG_yC
Cuts at: 1255 1503 2205 3701 1255
Size: 248 702 1496 1378
Fragments arranged by size:
1496 1378 702 248
BsaWI w'CCGG_w
Cuts at: 1013 1534 2212 2623 2770 3044 1013
Size: 521 678 411 147 274 1793
Fragments arranged by size:
1793 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 494 494
Size: 3824
BseYI C'CCAG_C
Cuts at: 1249 2007 2672 3080 1249
Size: 758 665 408 1993
Fragments arranged by size:
1993 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 654 654
Size: 3824
BsiEI CG_ry'CG
Cuts at: 104 1366 1412 2642 104
Size: 1262 46 1230 1286
Fragments arranged by size:
1286 1262 1230 46
BsiHKAI G_wGCw'C
Cuts at: 619 838 1616 1806 2666 619
Size: 219 778 190 860 1777
Fragments arranged by size:
1777 860 778 219 190
Bsp1286I G_dGCh'C
Cuts at: 130 177 515 619 696 838 1449 1542 1616
Size: 47 338 104 77 142 611 93 74
Cuts at: 1616 1806 1868 2666 130
Size: 190 62 798 1288
Fragments arranged by size:
1288 798 611 338 190 142 104 93
77 74 62 47
BspHI T'CATG_A
Cuts at: 540 581 2256 540
Size: 41 1675 2108
Fragments arranged by size:
2108 1675 41
BspMI ACCTGCnnnn'nnnn_
Cuts at: 874 1088 1390 1771 874
Size: 214 302 381 2927
Fragments arranged by size:
2927 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2116 2170 3654 3819 50
Size: 2066 54 1484 165 55
Fragments arranged by size:
2066 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 454 1736 454
Size: 1282 2542
BsrFI r'CCGG_y
Cuts at: 1822 2003 1822
Size: 181 3643
BssSI C'ACGA_G
Cuts at: 2095 2803 2095
Size: 708 3116
BstAPI GCAn_nnn'nTGC
Cuts at: 1201 1201
Size: 3824
BstBI TT'CG_AA
Cuts at: 2185 2185
Size: 3824
BstYI r'GATC_y
Cuts at: 1 849 1316 1339 1674 1920 2226 2238 2324
Size: 848 467 23 335 246 306 12 86
Cuts at: 2324 3376 1
Size: 1052 449
Fragments arranged by size:
1052 848 467 449 335 306 246 86
23 12
BtgI C'CryG_G
Cuts at: 203 203
Size: 3824
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1774 1955 2118 1774
Size: 181 163 3480
Fragments arranged by size:
3480 181 163
BtsI GCAGTG_nn'
Cuts at: 440 1067 440
Size: 627 3197
DraI TTT'AAA
Cuts at: 823 823
Size: 3824
DrdI GACnn_nn'nnGTC
Cuts at: 1530 2874 1530
Size: 1344 2480
EaeI y'GGCC_r
Cuts at: 1409 1583 1974 2001 3346 1409
Size: 174 391 27 1345 1887
Fragments arranged by size:
1887 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1409 1409
Size: 3824
EarI CTCTTCn'nnn_
Cuts at: 769 1847 2057 769
Size: 1078 210 2536
Fragments arranged by size:
2536 1078 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2032 2774 2920 3634 2032
Size: 742 146 714 2222
Fragments arranged by size:
2222 742 714 146
EcoICRI GAG'CTC
Cuts at: 836 836
Size: 3824
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 114 271 356 491 712 1649 2081 2434 3096
Size: 157 85 135 221 937 432 353 662
Cuts at: 3096 114
Size: 842
Fragments arranged by size:
937 842 662 432 353 221 157 135
85
EcoNI CCTnn'n_nnAGG
Cuts at: 427 427
Size: 3824
EcoO109I rG'GnC_Cy
Cuts at: 173 340 963 173
Size: 167 623 3034
Fragments arranged by size:
3034 623 167
EcoRI G'AATT_C
Cuts at: 828 828
Size: 3824
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1843 1843
Size: 3824
FspI TGC'GCA
Cuts at: 1605 1605
Size: 3824
HaeII r_GCGC'y
Cuts at: 1258 1506 2736 3507 1258
Size: 248 1230 771 1575
Fragments arranged by size:
1575 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 285 317 370 402 1666 1696 1698 1728 285
Size: 32 53 32 1264 30 2 30 2381
Fragments arranged by size:
2381 1264 53 32 32 30 30 2
HincII GTy'rAC
Cuts at: 863 863
Size: 3824
KasI G'GCGC_C
Cuts at: 1254 1502 1254
Size: 248 3576
KpnI G_GTAC'C
Cuts at: 844 844
Size: 3824
MluI A'CGCG_T
Cuts at: 3257 3257
Size: 3824
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2583 2767 2583
Size: 184 3640
MscI TGG'CCA
Cuts at: 1585 1585
Size: 3824
MslI CAynn'nnrTG
Cuts at: 1813 1940 1813
Size: 127 3697
MspA1I CmG'CkG
Cuts at: 231 935 1249 1609 2393 2638 231
Size: 704 314 360 784 245 1417
Fragments arranged by size:
1417 784 704 360 314 245
NaeI GCC'GGC
Cuts at: 2005 2005
Size: 3824
NarI GG'CG_CC
Cuts at: 1255 1503 1255
Size: 248 3576
NcoI C'CATG_G
Cuts at: 203 203
Size: 3824
NgoMIV G'CCGG_C
Cuts at: 2003 2003
Size: 3824
NspI r_CATG'y
Cuts at: 387 573 877 1377 2980 387
Size: 186 304 500 1603 1231
Fragments arranged by size:
1603 1231 500 304 186
PasI CC'CwG_GG
Cuts at: 123 123
Size: 3824
PciI A'CATG_T
Cuts at: 2976 2976
Size: 3824
PmeI GTTT'AAAC
Cuts at: 823 823
Size: 3824
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2270 2302 2270
Size: 32 3792
PpuMI rG'GwC_Cy
Cuts at: 340 340
Size: 3824
PsiI TTA'TAA
Cuts at: 355 355
Size: 3824
PspOMI G'GGCC_C
Cuts at: 173 173
Size: 3824
PstI C_TGCA'G
Cuts at: 871 1556 871
Size: 685 3139
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3824
PvuII CAG'CTG
Cuts at: 1249 1609 1249
Size: 360 3464
RsrII CG'GwC_CG
Cuts at: 2019 2019
Size: 3824
SacI G_AGCT'C
Cuts at: 838 838
Size: 3824
SalI G'TCGA_C
Cuts at: 861 861
Size: 3824
SapI GCTCTTCn'nnn_
Cuts at: 1847 2057 1847
Size: 210 3614
SbfI CC_TGCA'GG
Cuts at: 871 871
Size: 3824
ScaI AGT'ACT
Cuts at: 3341 3341
Size: 3824
SfcI C'TryA_G
Cuts at: 33 867 1552 2520 2711 33
Size: 834 685 968 191 1146
Fragments arranged by size:
1146 968 834 685 191
SfoI GGC'GCC
Cuts at: 1256 1504 1256
Size: 248 3576
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3824
SmaI CCC'GGG
Cuts at: 846 846
Size: 3824
SmlI C'TyrA_G
Cuts at: 979 1894 2608 2870 979
Size: 915 714 262 1933
Fragments arranged by size:
1933 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3824
SphI G_CATG'C
Cuts at: 877 1377 877
Size: 500 3324
StyI C'CwwG_G
Cuts at: 203 428 958 1312 203
Size: 225 530 354 2715
Fragments arranged by size:
2715 530 354 225
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 231 1407 1540 1999 2208 231
Size: 1176 133 459 209 1847
Fragments arranged by size:
1847 1176 459 209 133
TatI w'GTAC_w
Cuts at: 445 3339 445
Size: 2894 930
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3341 3373 3341
Size: 32 3792
Tth111I GACn'n_nGTC
Cuts at: 1621 1621
Size: 3824
XbaI T'CTAG_A
Cuts at: 855 855
Size: 3824
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1319 1319
Size: 3824
XmaI C'CCGG_G
Cuts at: 844 844
Size: 3824
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AhdI AloI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PmeI PpiI PpuMI PsiI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AleI AlfI AscI
AvrII BbvCI BfrBI BmgBI BmtI Bpu10I BsaXI BsiWI
BsmI BsmBI BspEI BsrGI BssHII BstEII BstXI BstZ17I
Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PflMI PfoI PmlI PshAI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI