Restriction map for
pF1KB8589
(Circular) (Six-base) MAPSORT of: pF1KB8589.seq Check: 5115 from: 1 to: 4187
pF1KB8589 4187 bp
With 182 enzymes: SgfI *
July 2, 2008 13:23 ..
AatII G_ACGT'C
Cuts at: 591 591
Size: 4187
AccI GT'mk_AC
Cuts at: 327 1225 327
Size: 898 3289
Acc65I G'GTAC_C
Cuts at: 936 1203 936
Size: 267 3920
AclI AA'CG_TT
Cuts at: 4027 4027
Size: 4187
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 911 1196 2012 2444 2797 3459 911
Size: 285 816 432 353 662 1639
Fragments arranged by size:
1639 816 662 432 353 285
AflIII A'CryG_T
Cuts at: 232 1539 3339 3620 232
Size: 1307 1800 281 799
Fragments arranged by size:
1800 1307 799 281
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 722 754 722
Size: 32 4155
AlwNI CAG_nnn'CTG
Cuts at: 1174 1492 2930 1174
Size: 318 1438 2431
Fragments arranged by size:
2431 1438 318
ApaLI G'TGCA_C
Cuts at: 1164 3025 1164
Size: 1861 2326
ApoI r'AATT_y
Cuts at: 335 1191 3540 3796 335
Size: 856 2349 256 726
Fragments arranged by size:
2349 856 726 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4187
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4187
AvaI C'yCGr_G
Cuts at: 340 1207 340
Size: 867 3320
BamHI G'GATC_C
Cuts at: 1212 1212
Size: 4187
BanI G'GyrC_C
Cuts at: 936 1203 1617 1865 1900 936
Size: 267 414 248 35 3223
Fragments arranged by size:
3223 414 267 248 35
BanII G_rGCy'C
Cuts at: 694 1201 2231 694
Size: 507 1030 2650
Fragments arranged by size:
2650 1030 507
BbeI G_GCGC'C
Cuts at: 1621 1869 1621
Size: 248 3939
BbsI GAAGACnn'nnnn_
Cuts at: 746 842 746
Size: 96 4091
BciVI GTATCCnnnnn_n'
Cuts at: 2085 3141 4187 2085
Size: 1056 1046 2085
Fragments arranged by size:
2085 1056 1046
BclI T'GATC_A
Cuts at: 129 1707 129
Size: 1578 2609
BfrBI ATG'CAT
Cuts at: 418 418
Size: 4187
BglII A'GATC_T
Cuts at: 1141 1702 3739 1141
Size: 561 2037 1589
Fragments arranged by size:
2037 1589 561
BlpI GC'TnA_GC
Cuts at: 1299 1299
Size: 4187
Bme1580I G_kGCm'C
Cuts at: 1168 1812 1905 3029 1168
Size: 644 93 1124 2326
Fragments arranged by size:
2326 1124 644 93
BmrI ACTGGGnnnn_n'
Cuts at: 1399 1480 1815 3959 1399
Size: 81 335 2144 1627
Fragments arranged by size:
2144 1627 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2554 2554
Size: 4187
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 370 1363 2242 2956 3254 370
Size: 993 879 714 298 1303
Fragments arranged by size:
1303 993 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 3891 3891
Size: 4187
BsaAI yAC'GTr
Cuts at: 1540 1540
Size: 4187
BsaBI GATnn'nnATC
Cuts at: 6 1701 3738 6
Size: 1695 2037 455
Fragments arranged by size:
2037 1695 455
BsaHI Gr'CG_yC
Cuts at: 588 1618 1866 2568 4064 588
Size: 1030 248 702 1496 711
Fragments arranged by size:
1496 1030 711 702 248
BsaWI w'CCGG_w
Cuts at: 1376 1897 2575 2986 3133 3407 1376
Size: 521 678 411 147 274 2156
Fragments arranged by size:
2156 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 722 752 722
Size: 30 4157
BseYI C'CCAG_C
Cuts at: 1612 2370 3035 3443 1612
Size: 758 665 408 2356
Fragments arranged by size:
2356 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 173 173
Size: 4187
BsiEI CG_ry'CG
Cuts at: 104 1077 1090 1729 1775 3005 104
Size: 973 13 639 46 1230 1286
Fragments arranged by size:
1286 1230 973 639 46 13
BsiHKAI G_wGCw'C
Cuts at: 321 694 1168 1201 1979 2169 3029 321
Size: 373 474 33 778 190 860 1479
Fragments arranged by size:
1479 860 778 474 373 190 33
Bsp1286I G_dGCh'C
Cuts at: 321 694 1168 1201 1812 1905 1979 2169 2231
Size: 373 474 33 611 93 74 190 62
Cuts at: 2231 3029 321
Size: 798 1479
Fragments arranged by size:
1479 798 611 474 373 190 93 74
62 33
BspHI T'CATG_A
Cuts at: 912 2619 912
Size: 1707 2480
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1237 1451 1753 2134 1237
Size: 214 302 381 3290
Fragments arranged by size:
3290 381 302 214
BsrBI CCG'CTC
Cuts at: 50 706 2479 2533 4017 4182 50
Size: 656 1773 54 1484 165 55
Fragments arranged by size:
1773 1484 656 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2099 2099
Size: 4187
BsrFI r'CCGG_y
Cuts at: 2185 2366 2185
Size: 181 4006
BssSI C'ACGA_G
Cuts at: 2458 3166 2458
Size: 708 3479
BstAPI GCAn_nnn'nTGC
Cuts at: 1012 1564 1012
Size: 552 3635
BstBI TT'CG_AA
Cuts at: 2548 2548
Size: 4187
BstYI r'GATC_y
Cuts at: 1 1141 1212 1679 1702 2037 2283 2589 2601
Size: 1140 71 467 23 335 246 306 12
Cuts at: 2601 2687 3739 1
Size: 86 1052 449
Fragments arranged by size:
1140 1052 467 449 335 306 246 86
71 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4187
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2137 2318 2481 2137
Size: 181 163 3843
Fragments arranged by size:
3843 181 163
BtsI GCAGTG_nn'
Cuts at: 1430 1430
Size: 4187
ClaI AT'CG_AT
Cuts at: 1077 1086 1101 1077
Size: 9 15 4163
Fragments arranged by size:
4163 15 9
DraI TTT'AAA
Cuts at: 1186 1186
Size: 4187
DraIII CAC_nnn'GTG
Cuts at: 727 727
Size: 4187
DrdI GACnn_nn'nnGTC
Cuts at: 1893 3237 1893
Size: 1344 2843
EaeI y'GGCC_r
Cuts at: 1772 1946 2337 2364 3709 1772
Size: 174 391 27 1345 2250
Fragments arranged by size:
2250 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1772 1772
Size: 4187
EarI CTCTTCn'nnn_
Cuts at: 2210 2420 2210
Size: 210 3977
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2395 3137 3283 3997 2395
Size: 742 146 714 2585
Fragments arranged by size:
2585 742 714 146
EcoICRI GAG'CTC
Cuts at: 692 1199 692
Size: 507 3680
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 911 1196 2012 2444 2797 3459 911
Size: 285 816 432 353 662 1639
Fragments arranged by size:
1639 816 662 432 353 285
EcoO109I rG'GnC_Cy
Cuts at: 764 1326 764
Size: 562 3625
EcoRI G'AATT_C
Cuts at: 1191 1191
Size: 4187
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 381 2206 381
Size: 1825 2362
FspI TGC'GCA
Cuts at: 1968 1968
Size: 4187
HaeII r_GCGC'y
Cuts at: 1621 1869 3099 3870 1621
Size: 248 1230 771 1938
Fragments arranged by size:
1938 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 700 732 901 933 985 1017 2029 2059 2061
Size: 32 169 32 52 32 1012 30 2
Cuts at: 2061 2091 700
Size: 30 2796
Fragments arranged by size:
2796 1012 169 52 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 328 791 1226 328
Size: 463 435 3289
Fragments arranged by size:
3289 463 435
HpaI GTT'AAC
Cuts at: 791 791
Size: 4187
KasI G'GCGC_C
Cuts at: 1617 1865 1617
Size: 248 3939
KpnI G_GTAC'C
Cuts at: 940 1207 940
Size: 267 3920
MluI A'CGCG_T
Cuts at: 3620 3620
Size: 4187
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2946 3130 2946
Size: 184 4003
MscI TGG'CCA
Cuts at: 1948 1948
Size: 4187
MslI CAynn'nnrTG
Cuts at: 514 2176 2303 514
Size: 1662 127 2398
Fragments arranged by size:
2398 1662 127
MspA1I CmG'CkG
Cuts at: 877 1298 1612 1972 2756 3001 877
Size: 421 314 360 784 245 2063
Fragments arranged by size:
2063 784 421 360 314 245
NaeI GCC'GGC
Cuts at: 2368 2368
Size: 4187
NarI GG'CG_CC
Cuts at: 1618 1866 1618
Size: 248 3939
NcoI C'CATG_G
Cuts at: 107 107
Size: 4187
NgoMIV G'CCGG_C
Cuts at: 2366 2366
Size: 4187
NsiI A_TGCA'T
Cuts at: 420 420
Size: 4187
NspI r_CATG'y
Cuts at: 147 236 1240 1740 3343 147
Size: 89 1004 500 1603 991
Fragments arranged by size:
1603 1004 991 500 89
PciI A'CATG_T
Cuts at: 232 3339 232
Size: 3107 1080
PmeI GTTT'AAAC
Cuts at: 1186 1186
Size: 4187
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 722 754 2633 2665 722
Size: 32 1879 32 2244
Fragments arranged by size:
2244 1879 32 32
PstI C_TGCA'G
Cuts at: 1008 1234 1919 1008
Size: 226 685 3276
Fragments arranged by size:
3276 685 226
PvuI CG_AT'CG
Cuts at: 104 1077 1090 104
Size: 973 13 3201
Fragments arranged by size:
3201 973 13
PvuII CAG'CTG
Cuts at: 877 1612 1972 877
Size: 735 360 3092
Fragments arranged by size:
3092 735 360
RsrII CG'GwC_CG
Cuts at: 2382 2382
Size: 4187
SacI G_AGCT'C
Cuts at: 694 1201 694
Size: 507 3680
SalI G'TCGA_C
Cuts at: 326 1224 326
Size: 898 3289
SapI GCTCTTCn'nnn_
Cuts at: 2210 2420 2210
Size: 210 3977
SbfI CC_TGCA'GG
Cuts at: 1234 1234
Size: 4187
ScaI AGT'ACT
Cuts at: 3704 3704
Size: 4187
SfcI C'TryA_G
Cuts at: 33 1004 1230 1915 2883 3074 33
Size: 971 226 685 968 191 1146
Fragments arranged by size:
1146 971 968 685 226 191
SfoI GGC'GCC
Cuts at: 1619 1867 1619
Size: 248 3939
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4187
SmaI CCC'GGG
Cuts at: 1209 1209
Size: 4187
SmlI C'TyrA_G
Cuts at: 385 1342 2257 2971 3233 385
Size: 957 915 714 262 1339
Fragments arranged by size:
1339 957 915 714 262
SpeI A'CTAG_T
Cuts at: 67 466 67
Size: 399 3788
SphI G_CATG'C
Cuts at: 1240 1740 1240
Size: 500 3687
StuI AGG'CCT
Cuts at: 1152 1152
Size: 4187
StyI C'CwwG_G
Cuts at: 107 1321 1675 107
Size: 1214 354 2619
Fragments arranged by size:
2619 1214 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 431 1770 1903 2362 2571 431
Size: 1339 133 459 209 2047
Fragments arranged by size:
2047 1339 459 209 133
TatI w'GTAC_w
Cuts at: 3702 3702
Size: 4187
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3704 3736 3704
Size: 32 4155
Tth111I GACn'n_nGTC
Cuts at: 1984 1984
Size: 4187
XbaI T'CTAG_A
Cuts at: 1144 1218 1144
Size: 74 4113
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1682 1682
Size: 4187
XmaI C'CCGG_G
Cuts at: 1207 1207
Size: 4187
ZraI GAC'GTC
Cuts at: 589 589
Size: 4187
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BfrBI BglII BlpI Bme1580I
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI ClaI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HpaI KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI
NspI PciI PmeI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AgeI AhdI AleI AlfI ApaI
AscI AvrII BaeI BbvCI BcgI BglI BmgBI BmtI
BpmI Bpu10I BseRI BsiWI BsmI BsmBI BspEI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I CspCI EcoNI EcoRV
FseI FspAI HindIII MfeI NdeI NheI NotI NruI
PacI PasI PflMI PfoI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI XmnI