Restriction map for
pF1KB8595
(Circular) (Six-base) MAPSORT of: pF1KB8595.seq Check: 7997 from: 1 to: 9719
pF1KB8595 9719 bp
With 182 enzymes: SgfI *
March 2, 2010 14:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1998 1998
Size: 9719
AatII G_ACGT'C
Cuts at: 1767 4806 5394 1767
Size: 3039 588 6092
Fragments arranged by size:
6092 3039 588
AccI GT'mk_AC
Cuts at: 6757 6757
Size: 9719
Acc65I G'GTAC_C
Cuts at: 6735 6735
Size: 9719
AclI AA'CG_TT
Cuts at: 3408 6641 9559 3408
Size: 3233 2918 3568
Fragments arranged by size:
3568 3233 2918
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 845 883 950 1358 1740 3182 3637 4006 4062
Size: 38 67 408 382 1442 455 369 56
Cuts at: 4062 4871 5654 7544 7976 8329 8991 845
Size: 809 783 1890 432 353 662 1573
Fragments arranged by size:
1890 1573 1442 809 783 662 455 432
408 382 369 353 67 56 38
AflIII A'CryG_T
Cuts at: 2091 7071 8871 9152 2091
Size: 4980 1800 281 2658
Fragments arranged by size:
4980 2658 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 3619 3619
Size: 9719
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1304 1304
Size: 9719
AlwNI CAG_nnn'CTG
Cuts at: 1615 2778 3343 3925 4418 7024 8462 1615
Size: 1163 565 582 493 2606 1438 2872
Fragments arranged by size:
2872 2606 1438 1163 582 565 493
ApaI G_GGCC'C
Cuts at: 6061 6061
Size: 9719
ApaLI G'TGCA_C
Cuts at: 8557 8557
Size: 9719
ApoI r'AATT_y
Cuts at: 262 603 801 1043 1655 1696 1829 2481 2633
Size: 341 198 242 612 41 133 652 152
Cuts at: 2633 2686 3166 4527 5251 6189 6723 9072 9328
Size: 53 480 1361 724 938 534 2349 256
Cuts at: 9328 262
Size: 653
Fragments arranged by size:
2349 1361 938 724 653 652 612 534
480 341 256 242 198 152 133 53
41
AseI AT'TA_AT
Cuts at: 20 20
Size: 9719
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 9719
AvaI C'yCGr_G
Cuts at: 6739 6739
Size: 9719
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2386 2419 2386
Size: 33 9686
BamHI G'GATC_C
Cuts at: 6744 6744
Size: 9719
BanI G'GyrC_C
Cuts at: 6735 7149 7397 7432 6735
Size: 414 248 35 9022
Fragments arranged by size:
9022 414 248 35
BanII G_rGCy'C
Cuts at: 6061 6733 7763 6061
Size: 672 1030 8017
Fragments arranged by size:
8017 1030 672
BbeI G_GCGC'C
Cuts at: 7153 7401 7153
Size: 248 9471
BbsI GAAGACnn'nnnn_
Cuts at: 317 4197 5722 317
Size: 3880 1525 4314
Fragments arranged by size:
4314 3880 1525
BbvCI CC'TCA_GC
Cuts at: 3944 3944
Size: 9719
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1639 1673 1639
Size: 34 9685
BciVI GTATCCnnnnn_n'
Cuts at: 1220 7617 8673 9719 1220
Size: 6397 1056 1046 1220
Fragments arranged by size:
6397 1220 1056 1046
BclI T'GATC_A
Cuts at: 1005 3171 3735 3795 7239 1005
Size: 2166 564 60 3444 3485
Fragments arranged by size:
3485 3444 2166 564 60
BfrBI ATG'CAT
Cuts at: 627 4962 5356 627
Size: 4335 394 4990
Fragments arranged by size:
4990 4335 394
BglI GCCn_nnn'nGGC
Cuts at: 4843 4843
Size: 9719
BglII A'GATC_T
Cuts at: 843 7234 9271 843
Size: 6391 2037 1291
Fragments arranged by size:
6391 2037 1291
BlpI GC'TnA_GC
Cuts at: 6831 6831
Size: 9719
Bme1580I G_kGCm'C
Cuts at: 1605 6061 7344 7437 8561 1605
Size: 4456 1283 93 1124 2763
Fragments arranged by size:
4456 2763 1283 1124 93
BmgBI CAC'GTC
Cuts at: 1132 5895 1132
Size: 4763 4956
BmrI ACTGGGnnnn_n'
Cuts at: 4657 5266 6931 7012 7347 9491 4657
Size: 609 1665 81 335 2144 4885
Fragments arranged by size:
4885 2144 1665 609 335 81
BmtI G_CTAG'C
Cuts at: 3482 3482
Size: 9719
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3721 4013 8086 3721
Size: 292 4073 5354
Fragments arranged by size:
5354 4073 292
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 301 703 943 1190 3269 4751 5143 301
Size: 402 240 247 2079 1482 392 4877
Fragments arranged by size:
4877 2079 1482 402 392 247 240
Bpu10I CC'TnA_GC
Cuts at: 3944 3944
Size: 9719
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 727 4915 6895 7774 8488 8786 727
Size: 4188 1980 879 714 298 1660
Fragments arranged by size:
4188 1980 1660 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 3088 4592 6687 9423 3088
Size: 1504 2095 2736 3384
Fragments arranged by size:
3384 2736 2095 1504
BsaAI yAC'GTr
Cuts at: 4264 7072 4264
Size: 2808 6911
BsaBI GATnn'nnATC
Cuts at: 6 5956 7233 9270 6
Size: 5950 1277 2037 455
Fragments arranged by size:
5950 2037 1277 455
BsaHI Gr'CG_yC
Cuts at: 1764 4803 5391 7150 7398 8100 9596 1764
Size: 3039 588 1759 248 702 1496 1887
Fragments arranged by size:
3039 1887 1759 1496 702 588 248
BsaWI w'CCGG_w
Cuts at: 6908 7429 8107 8518 8665 8939 6908
Size: 521 678 411 147 274 7688
Fragments arranged by size:
7688 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 3310 3340 3310
Size: 30 9689
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 946 3031 3217 3323 3989 6410 946
Size: 2085 186 106 666 2421 4255
Fragments arranged by size:
4255 2421 2085 666 186 106
BseYI C'CCAG_C
Cuts at: 921 5176 5407 7144 7902 8567 8975 921
Size: 4255 231 1737 758 665 408 1665
Fragments arranged by size:
4255 1737 1665 758 665 408 231
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 577 6431 6647 577
Size: 5854 216 3649
Fragments arranged by size:
5854 3649 216
BsiEI CG_ry'CG
Cuts at: 104 7261 7307 8537 104
Size: 7157 46 1230 1286
Fragments arranged by size:
7157 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 3384 4155 5300 6733 7511 7701 8561 3384
Size: 771 1145 1433 778 190 860 4542
Fragments arranged by size:
4542 1433 1145 860 778 771 190
BsmI GAATG_Cn'
Cuts at: 1257 2468 3366 4964 5629 1257
Size: 1211 898 1598 665 5347
Fragments arranged by size:
5347 1598 1211 898 665
BsmBI CGTCTCn'nnnn_
Cuts at: 127 127
Size: 9719
Bsp1286I G_dGCh'C
Cuts at: 1605 3384 4155 5300 6061 6733 7344 7437 7511
Size: 1779 771 1145 761 672 611 93 74
Cuts at: 7511 7701 7763 8561 1605
Size: 190 62 798 2763
Fragments arranged by size:
2763 1779 1145 798 771 761 672 611
190 93 74 62
BspHI T'CATG_A
Cuts at: 216 401 5223 5399 6590 8151 216
Size: 185 4822 176 1191 1561 1784
Fragments arranged by size:
4822 1784 1561 1191 185 176
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1998 6769 6983 7285 7666 1998
Size: 4771 214 302 381 4051
Fragments arranged by size:
4771 4051 381 302 214
BsrBI CCG'CTC
Cuts at: 50 8011 8065 9549 9714 50
Size: 7961 54 1484 165 55
Fragments arranged by size:
7961 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1628 1640 4358 4904 5891 7631 1628
Size: 12 2718 546 987 1740 3716
Fragments arranged by size:
3716 2718 1740 987 546 12
BsrFI r'CCGG_y
Cuts at: 7717 7898 7717
Size: 181 9538
BsrGI T'GTAC_A
Cuts at: 2271 2530 4070 4095 2271
Size: 259 1540 25 7895
Fragments arranged by size:
7895 1540 259 25
BssSI C'ACGA_G
Cuts at: 6240 7990 8698 6240
Size: 1750 708 7261
Fragments arranged by size:
7261 1750 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1363 2812 3352 3361 5323 7096 1363
Size: 1449 540 9 1962 1773 3986
Fragments arranged by size:
3986 1962 1773 1449 540 9
BstBI TT'CG_AA
Cuts at: 6516 8080 6516
Size: 1564 8155
BstXI CCAn_nnnn'nTGG
Cuts at: 1868 4397 4480 4868 6115 1868
Size: 2529 83 388 1247 5472
Fragments arranged by size:
5472 2529 1247 388 83
BstYI r'GATC_y
Cuts at: 1 843 3783 4017 4469 4857 6744 7211 7234
Size: 842 2940 234 452 388 1887 467 23
Cuts at: 7234 7569 7815 8121 8133 8219 9271 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
2940 1887 1052 842 467 452 449 388
335 306 246 234 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1214 1214
Size: 9719
BtgI C'CryG_G
Cuts at: 107 6143 107
Size: 6036 3683
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 7669 7850 8013 7669
Size: 181 163 9375
Fragments arranged by size:
9375 181 163
BtsI GCAGTG_nn'
Cuts at: 488 2852 3926 4873 6962 488
Size: 2364 1074 947 2089 3245
Fragments arranged by size:
3245 2364 2089 1074 947
DraI TTT'AAA
Cuts at: 1521 6718 1521
Size: 5197 4522
DraIII CAC_nnn'GTG
Cuts at: 2259 2259
Size: 9719
DrdI GACnn_nn'nnGTC
Cuts at: 4378 7425 8769 4378
Size: 3047 1344 5328
Fragments arranged by size:
5328 3047 1344
EaeI y'GGCC_r
Cuts at: 2127 2334 5553 7304 7478 7869 7896 9241 2127
Size: 207 3219 1751 174 391 27 1345 2605
Fragments arranged by size:
3219 2605 1751 1345 391 207 174 27
EagI C'GGCC_G
Cuts at: 7304 7304
Size: 9719
EarI CTCTTCn'nnn_
Cuts at: 280 403 1028 1525 3185 5988 5993 7742 7952
Size: 123 625 497 1660 2803 5 1749 210
Cuts at: 7952 280
Size: 2047
Fragments arranged by size:
2803 2047 1749 1660 625 497 210 123
5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 7927 8669 8815 9529 7927
Size: 742 146 714 8117
Fragments arranged by size:
8117 742 714 146
EcoICRI GAG'CTC
Cuts at: 6731 6731
Size: 9719
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 301 703 845 883 943 950 1190 1358 1740
Size: 402 142 38 60 7 240 168 382
Cuts at: 1740 3182 3269 3637 4006 4062 4751 4871 5143
Size: 1442 87 368 369 56 689 120 272
Cuts at: 5143 5654 7544 7976 8329 8991 301
Size: 511 1890 432 353 662 1029
Fragments arranged by size:
1890 1442 1029 689 662 511 432 402
382 369 368 353 272 240 168 142
120 87 60 56 38 7
EcoNI CCTnn'n_nnAGG
Cuts at: 5791 5791
Size: 9719
EcoO109I rG'GnC_Cy
Cuts at: 925 4495 6057 6858 925
Size: 3570 1562 801 3786
Fragments arranged by size:
3786 3570 1562 801
EcoRI G'AATT_C
Cuts at: 6723 6723
Size: 9719
EcoRV GAT'ATC
Cuts at: 3138 3138
Size: 9719
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2882 4321 5256 5628 5659 6673 7738 2882
Size: 1439 935 372 31 1014 1065 4863
Fragments arranged by size:
4863 1439 1065 1014 935 372 31
FspI TGC'GCA
Cuts at: 7500 7500
Size: 9719
HaeII r_GCGC'y
Cuts at: 7153 7401 8631 9402 7153
Size: 248 1230 771 7470
Fragments arranged by size:
7470 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2643 2675 2877 2909 3310 3342 3605 3637 4339
Size: 32 202 32 401 32 263 32 702
Cuts at: 4339 4371 5381 5413 5949 5981 7561 7591 7593
Size: 32 1010 32 536 32 1580 30 2
Cuts at: 7593 7623 2643
Size: 30 4739
Fragments arranged by size:
4739 1580 1010 702 536 401 263 202
32 32 32 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2625 4702 6672 6758 2625
Size: 2077 1970 86 5586
Fragments arranged by size:
5586 2077 1970 86
HindIII A'AGCT_T
Cuts at: 1414 3541 1414
Size: 2127 7592
KasI G'GCGC_C
Cuts at: 7149 7397 7149
Size: 248 9471
KpnI G_GTAC'C
Cuts at: 6739 6739
Size: 9719
MfeI C'AATT_G
Cuts at: 374 974 1032 2762 374
Size: 600 58 1730 7331
Fragments arranged by size:
7331 1730 600 58
MluI A'CGCG_T
Cuts at: 9152 9152
Size: 9719
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 330 2958 4548 4671 4949 5412 6055 8478 8662
Size: 2628 1590 123 278 463 643 2423 184
Cuts at: 8662 330
Size: 1387
Fragments arranged by size:
2628 2423 1590 1387 643 463 278 184
123
MscI TGG'CCA
Cuts at: 2336 7480 2336
Size: 5144 4575
MslI CAynn'nnrTG
Cuts at: 1322 1633 1786 2372 5312 6113 7708 7835 1322
Size: 311 153 586 2940 801 1595 127 3206
Fragments arranged by size:
3206 2940 1595 801 586 311 153 127
MspA1I CmG'CkG
Cuts at: 1512 1738 4183 6830 7144 7504 8288 8533 1512
Size: 226 2445 2647 314 360 784 245 2698
Fragments arranged by size:
2698 2647 2445 784 360 314 245 226
NaeI GCC'GGC
Cuts at: 7900 7900
Size: 9719
NarI GG'CG_CC
Cuts at: 7150 7398 7150
Size: 248 9471
NcoI C'CATG_G
Cuts at: 107 6143 107
Size: 6036 3683
NdeI CA'TA_TG
Cuts at: 2904 3348 4059 5705 2904
Size: 444 711 1646 6918
Fragments arranged by size:
6918 1646 711 444
NgoMIV G'CCGG_C
Cuts at: 7898 7898
Size: 9719
NheI G'CTAG_C
Cuts at: 3478 3478
Size: 9719
NsiI A_TGCA'T
Cuts at: 629 4964 5358 629
Size: 4335 394 4990
Fragments arranged by size:
4990 4335 394
NspI r_CATG'y
Cuts at: 334 627 1593 1812 2095 2278 6067 6166 6334
Size: 293 966 219 283 183 3789 99 168
Cuts at: 6334 6772 7272 8875 334
Size: 438 500 1603 1178
Fragments arranged by size:
3789 1603 1178 966 500 438 293 283
219 183 168 99
PciI A'CATG_T
Cuts at: 2091 8871 2091
Size: 6780 2939
PflMI CCAn_nnn'nTGG
Cuts at: 661 2107 2262 3864 5166 661
Size: 1446 155 1602 1302 5214
Fragments arranged by size:
5214 1602 1446 1302 155
PfoI T'CCnGG_A
Cuts at: 719 3246 719
Size: 2527 7192
PmeI GTTT'AAAC
Cuts at: 6718 6718
Size: 9719
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4484 4516 8165 8197 4484
Size: 32 3649 32 6006
Fragments arranged by size:
6006 3649 32 32
PpuMI rG'GwC_Cy
Cuts at: 925 925
Size: 9719
PsiI TTA'TAA
Cuts at: 173 695 2707 5246 173
Size: 522 2012 2539 4646
Fragments arranged by size:
4646 2539 2012 522
PspOMI G'GGCC_C
Cuts at: 6057 6057
Size: 9719
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4649 4681 5204 5236 4649
Size: 32 523 32 9132
Fragments arranged by size:
9132 523 32 32
PstI C_TGCA'G
Cuts at: 495 6297 6766 7451 495
Size: 5802 469 685 2763
Fragments arranged by size:
5802 2763 685 469
PvuI CG_AT'CG
Cuts at: 104 104
Size: 9719
PvuII CAG'CTG
Cuts at: 1512 1738 4183 7144 7504 1512
Size: 226 2445 2961 360 3727
Fragments arranged by size:
3727 2961 2445 360 226
RsrII CG'GwC_CG
Cuts at: 7914 7914
Size: 9719
SacI G_AGCT'C
Cuts at: 6733 6733
Size: 9719
SalI G'TCGA_C
Cuts at: 6756 6756
Size: 9719
SapI GCTCTTCn'nnn_
Cuts at: 1028 7742 7952 1028
Size: 6714 210 2795
Fragments arranged by size:
6714 2795 210
SbfI CC_TGCA'GG
Cuts at: 6766 6766
Size: 9719
ScaI AGT'ACT
Cuts at: 5631 9236 5631
Size: 3605 6114
SexAI A'CCwGG_T
Cuts at: 2255 2255
Size: 9719
SfcI C'TryA_G
Cuts at: 33 491 1440 1607 3426 5791 6293 6762 7447
Size: 458 949 167 1819 2365 502 469 685
Cuts at: 7447 8415 8606 33
Size: 968 191 1146
Fragments arranged by size:
2365 1819 1146 968 949 685 502 469
458 191 167
SfoI GGC'GCC
Cuts at: 7151 7399 7151
Size: 248 9471
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 9719
SmaI CCC'GGG
Cuts at: 6741 6741
Size: 9719
SmlI C'TyrA_G
Cuts at: 742 4930 6874 7789 8503 8765 742
Size: 4188 1944 915 714 262 1696
Fragments arranged by size:
4188 1944 1696 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 9719
SphI G_CATG'C
Cuts at: 6334 6772 7272 6334
Size: 438 500 8781
Fragments arranged by size:
8781 500 438
SspI AAT'ATT
Cuts at: 3609 4109 4620 3609
Size: 500 511 8708
Fragments arranged by size:
8708 511 500
StyI C'CwwG_G
Cuts at: 107 1730 2361 2628 3763 4364 5199 6143 6606
Size: 1623 631 267 1135 601 835 944 463
Cuts at: 6606 6677 6853 7207 107
Size: 71 176 354 2619
Fragments arranged by size:
2619 1623 1135 944 835 631 601 463
354 267 176 71
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3928 5003 5413 7302 7435 7894 8103 3928
Size: 1075 410 1889 133 459 209 5544
Fragments arranged by size:
5544 1889 1075 459 410 209 133
TatI w'GTAC_w
Cuts at: 853 2271 2376 2530 4070 4095 5629 9234 853
Size: 1418 105 154 1540 25 1534 3605 1338
Fragments arranged by size:
3605 1540 1534 1418 1338 154 105 25
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2160 2192 2243 2275 3500 3532 9236 9268 2160
Size: 32 51 32 1225 32 5704 32 2611
Fragments arranged by size:
5704 2611 1225 51 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 7516 7516
Size: 9719
XbaI T'CTAG_A
Cuts at: 1767 4503 6750 1767
Size: 2736 2247 4736
Fragments arranged by size:
4736 2736 2247
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 5853 7214 5853
Size: 1361 8358
XmaI C'CCGG_G
Cuts at: 6739 6739
Size: 9719
XmnI GAAnn'nnTTC
Cuts at: 519 4761 519
Size: 4242 5477
ZraI GAC'GTC
Cuts at: 1765 4804 5392 1765
Size: 3039 588 6092
Fragments arranged by size:
6092 3039 588
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AhdI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NsiI NspI PciI PflMI
PfoI PmeI PpiI PpuMI PsiI PspOMI PsrI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AgeI AleI AloI AscI AvrII BsiWI
BspEI BssHII BstEII BstZ17I ClaI CspCI FseI FspAI
HpaI NotI NruI PacI PasI PmlI PshAI PspXI
SacII SanDI SfiI SgrAI SnaBI SrfI StuI SwaI
XhoI