FASTA searches a protein or DNA sequence data bank 36.3.4 Apr, 2011 Please cite: W.R. Pearson & D.J. Lipman PNAS (1988) 85:2444-2448 Query: pF1KB8597, 254 aa 1>>>pF1KB8597 254 - 254 aa - 254 aa Library: /omim/omim.rfq.tfa 60827320 residues in 85289 sequences Statistics: Expectation_n fit: rho(ln(x))= 7.0924+/-0.00033; mu= 5.9697+/- 0.021 mean_var=105.4764+/-21.242, 0's: 0 Z-trim(118.4): 50 B-trim: 948 in 1/55 Lambda= 0.124881 statistics sampled from 31320 (31370) to 31320 sequences Algorithm: FASTA (3.7 Nov 2010) [optimized] Parameters: BL50 matrix (15:-5), open/ext: -10/-2 ktup: 2, E-join: 1 (0.751), E-opt: 0.2 (0.368), width: 16 Scan time: 7.720 The best scores are: opt bits E(85289) NP_001257367 (OMIM: 606133) cdc42 effector protein ( 254) 1697 315.8 4.6e-86 NP_006440 (OMIM: 606133) cdc42 effector protein 3 ( 254) 1697 315.8 4.6e-86 NP_001257366 (OMIM: 606133) cdc42 effector protein ( 254) 1697 315.8 4.6e-86 XP_011530784 (OMIM: 606133) PREDICTED: cdc42 effec ( 254) 1697 315.8 4.6e-86 NP_001257365 (OMIM: 606133) cdc42 effector protein ( 254) 1697 315.8 4.6e-86 NP_006770 (OMIM: 606132) cdc42 effector protein 2 ( 210) 444 90.1 3.5e-18 XP_016872583 (OMIM: 606132) PREDICTED: cdc42 effec ( 210) 444 90.1 3.5e-18 NP_689449 (OMIM: 606084) cdc42 effector protein 1 ( 391) 200 46.2 0.0001 XP_006724172 (OMIM: 606084) PREDICTED: cdc42 effec ( 391) 200 46.2 0.0001 XP_006724170 (OMIM: 606084) PREDICTED: cdc42 effec ( 391) 200 46.2 0.0001 XP_006724171 (OMIM: 606084) PREDICTED: cdc42 effec ( 391) 200 46.2 0.0001 XP_016884030 (OMIM: 606084) PREDICTED: cdc42 effec ( 391) 200 46.2 0.0001 NP_036253 (OMIM: 605468) cdc42 effector protein 4 ( 356) 189 44.2 0.00038 XP_005257239 (OMIM: 605468) PREDICTED: cdc42 effec ( 356) 189 44.2 0.00038 >>NP_001257367 (OMIM: 606133) cdc42 effector protein 3 [ (254 aa) initn: 1697 init1: 1697 opt: 1697 Z-score: 1666.1 bits: 315.8 E(85289): 4.6e-86 Smith-Waterman score: 1697; 100.0% identity (100.0% similar) in 254 aa overlap (1-254:1-254) 10 20 30 40 50 60 pF1KB8 MPAKTPIYLKAANNKKGKKFKLRDILSPDMISPPLGDFRHTIHIGKEGQHDVFGDISFLQ :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: NP_001 MPAKTPIYLKAANNKKGKKFKLRDILSPDMISPPLGDFRHTIHIGKEGQHDVFGDISFLQ 10 20 30 40 50 60 70 80 90 100 110 120 pF1KB8 GNYELLPGNQEKAHLGQFPGHNEFFRANSTSDSVFTETPSPVLKNAISLPTIGGSQALML :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: NP_001 GNYELLPGNQEKAHLGQFPGHNEFFRANSTSDSVFTETPSPVLKNAISLPTIGGSQALML 70 80 90 100 110 120 130 140 150 160 170 180 pF1KB8 PLLSPVTFNSKQESFGPAKLPRLSCEPVMEEKAQEKSSLLENGTVHQGDTSWGSSGSASQ :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: NP_001 PLLSPVTFNSKQESFGPAKLPRLSCEPVMEEKAQEKSSLLENGTVHQGDTSWGSSGSASQ 130 140 150 160 170 180 190 200 210 220 230 240 pF1KB8 SSQGRDSHSSSLSEQYPDWPAEDMFDHPTPCELIKGKTKSEESLSDLTGSLLSLQLDLGP :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: NP_001 SSQGRDSHSSSLSEQYPDWPAEDMFDHPTPCELIKGKTKSEESLSDLTGSLLSLQLDLGP 190 200 210 220 230 240 250 pF1KB8 SLLDEVLNVMDKNK :::::::::::::: NP_001 SLLDEVLNVMDKNK 250 >>NP_006440 (OMIM: 606133) cdc42 effector protein 3 [Hom (254 aa) initn: 1697 init1: 1697 opt: 1697 Z-score: 1666.1 bits: 315.8 E(85289): 4.6e-86 Smith-Waterman score: 1697; 100.0% identity (100.0% similar) in 254 aa overlap (1-254:1-254) 10 20 30 40 50 60 pF1KB8 MPAKTPIYLKAANNKKGKKFKLRDILSPDMISPPLGDFRHTIHIGKEGQHDVFGDISFLQ :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: NP_006 MPAKTPIYLKAANNKKGKKFKLRDILSPDMISPPLGDFRHTIHIGKEGQHDVFGDISFLQ 10 20 30 40 50 60 70 80 90 100 110 120 pF1KB8 GNYELLPGNQEKAHLGQFPGHNEFFRANSTSDSVFTETPSPVLKNAISLPTIGGSQALML :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: NP_006 GNYELLPGNQEKAHLGQFPGHNEFFRANSTSDSVFTETPSPVLKNAISLPTIGGSQALML 70 80 90 100 110 120 130 140 150 160 170 180 pF1KB8 PLLSPVTFNSKQESFGPAKLPRLSCEPVMEEKAQEKSSLLENGTVHQGDTSWGSSGSASQ :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: NP_006 PLLSPVTFNSKQESFGPAKLPRLSCEPVMEEKAQEKSSLLENGTVHQGDTSWGSSGSASQ 130 140 150 160 170 180 190 200 210 220 230 240 pF1KB8 SSQGRDSHSSSLSEQYPDWPAEDMFDHPTPCELIKGKTKSEESLSDLTGSLLSLQLDLGP :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: NP_006 SSQGRDSHSSSLSEQYPDWPAEDMFDHPTPCELIKGKTKSEESLSDLTGSLLSLQLDLGP 190 200 210 220 230 240 250 pF1KB8 SLLDEVLNVMDKNK :::::::::::::: NP_006 SLLDEVLNVMDKNK 250 >>NP_001257366 (OMIM: 606133) cdc42 effector protein 3 [ (254 aa) initn: 1697 init1: 1697 opt: 1697 Z-score: 1666.1 bits: 315.8 E(85289): 4.6e-86 Smith-Waterman score: 1697; 100.0% identity (100.0% similar) in 254 aa overlap (1-254:1-254) 10 20 30 40 50 60 pF1KB8 MPAKTPIYLKAANNKKGKKFKLRDILSPDMISPPLGDFRHTIHIGKEGQHDVFGDISFLQ :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: NP_001 MPAKTPIYLKAANNKKGKKFKLRDILSPDMISPPLGDFRHTIHIGKEGQHDVFGDISFLQ 10 20 30 40 50 60 70 80 90 100 110 120 pF1KB8 GNYELLPGNQEKAHLGQFPGHNEFFRANSTSDSVFTETPSPVLKNAISLPTIGGSQALML :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: NP_001 GNYELLPGNQEKAHLGQFPGHNEFFRANSTSDSVFTETPSPVLKNAISLPTIGGSQALML 70 80 90 100 110 120 130 140 150 160 170 180 pF1KB8 PLLSPVTFNSKQESFGPAKLPRLSCEPVMEEKAQEKSSLLENGTVHQGDTSWGSSGSASQ :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: NP_001 PLLSPVTFNSKQESFGPAKLPRLSCEPVMEEKAQEKSSLLENGTVHQGDTSWGSSGSASQ 130 140 150 160 170 180 190 200 210 220 230 240 pF1KB8 SSQGRDSHSSSLSEQYPDWPAEDMFDHPTPCELIKGKTKSEESLSDLTGSLLSLQLDLGP :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: NP_001 SSQGRDSHSSSLSEQYPDWPAEDMFDHPTPCELIKGKTKSEESLSDLTGSLLSLQLDLGP 190 200 210 220 230 240 250 pF1KB8 SLLDEVLNVMDKNK :::::::::::::: NP_001 SLLDEVLNVMDKNK 250 >>XP_011530784 (OMIM: 606133) PREDICTED: cdc42 effector (254 aa) initn: 1697 init1: 1697 opt: 1697 Z-score: 1666.1 bits: 315.8 E(85289): 4.6e-86 Smith-Waterman score: 1697; 100.0% identity (100.0% similar) in 254 aa overlap (1-254:1-254) 10 20 30 40 50 60 pF1KB8 MPAKTPIYLKAANNKKGKKFKLRDILSPDMISPPLGDFRHTIHIGKEGQHDVFGDISFLQ :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: XP_011 MPAKTPIYLKAANNKKGKKFKLRDILSPDMISPPLGDFRHTIHIGKEGQHDVFGDISFLQ 10 20 30 40 50 60 70 80 90 100 110 120 pF1KB8 GNYELLPGNQEKAHLGQFPGHNEFFRANSTSDSVFTETPSPVLKNAISLPTIGGSQALML :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: XP_011 GNYELLPGNQEKAHLGQFPGHNEFFRANSTSDSVFTETPSPVLKNAISLPTIGGSQALML 70 80 90 100 110 120 130 140 150 160 170 180 pF1KB8 PLLSPVTFNSKQESFGPAKLPRLSCEPVMEEKAQEKSSLLENGTVHQGDTSWGSSGSASQ :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: XP_011 PLLSPVTFNSKQESFGPAKLPRLSCEPVMEEKAQEKSSLLENGTVHQGDTSWGSSGSASQ 130 140 150 160 170 180 190 200 210 220 230 240 pF1KB8 SSQGRDSHSSSLSEQYPDWPAEDMFDHPTPCELIKGKTKSEESLSDLTGSLLSLQLDLGP :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: XP_011 SSQGRDSHSSSLSEQYPDWPAEDMFDHPTPCELIKGKTKSEESLSDLTGSLLSLQLDLGP 190 200 210 220 230 240 250 pF1KB8 SLLDEVLNVMDKNK :::::::::::::: XP_011 SLLDEVLNVMDKNK 250 >>NP_001257365 (OMIM: 606133) cdc42 effector protein 3 [ (254 aa) initn: 1697 init1: 1697 opt: 1697 Z-score: 1666.1 bits: 315.8 E(85289): 4.6e-86 Smith-Waterman score: 1697; 100.0% identity (100.0% similar) in 254 aa overlap (1-254:1-254) 10 20 30 40 50 60 pF1KB8 MPAKTPIYLKAANNKKGKKFKLRDILSPDMISPPLGDFRHTIHIGKEGQHDVFGDISFLQ :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: NP_001 MPAKTPIYLKAANNKKGKKFKLRDILSPDMISPPLGDFRHTIHIGKEGQHDVFGDISFLQ 10 20 30 40 50 60 70 80 90 100 110 120 pF1KB8 GNYELLPGNQEKAHLGQFPGHNEFFRANSTSDSVFTETPSPVLKNAISLPTIGGSQALML :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: NP_001 GNYELLPGNQEKAHLGQFPGHNEFFRANSTSDSVFTETPSPVLKNAISLPTIGGSQALML 70 80 90 100 110 120 130 140 150 160 170 180 pF1KB8 PLLSPVTFNSKQESFGPAKLPRLSCEPVMEEKAQEKSSLLENGTVHQGDTSWGSSGSASQ :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: NP_001 PLLSPVTFNSKQESFGPAKLPRLSCEPVMEEKAQEKSSLLENGTVHQGDTSWGSSGSASQ 130 140 150 160 170 180 190 200 210 220 230 240 pF1KB8 SSQGRDSHSSSLSEQYPDWPAEDMFDHPTPCELIKGKTKSEESLSDLTGSLLSLQLDLGP :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: NP_001 SSQGRDSHSSSLSEQYPDWPAEDMFDHPTPCELIKGKTKSEESLSDLTGSLLSLQLDLGP 190 200 210 220 230 240 250 pF1KB8 SLLDEVLNVMDKNK :::::::::::::: NP_001 SLLDEVLNVMDKNK 250 >>NP_006770 (OMIM: 606132) cdc42 effector protein 2 [Hom (210 aa) initn: 540 init1: 293 opt: 444 Z-score: 447.4 bits: 90.1 E(85289): 3.5e-18 Smith-Waterman score: 493; 43.1% identity (61.6% similar) in 255 aa overlap (1-253:1-202) 10 20 30 40 50 60 pF1KB8 MPAKTPIYLKAANNKKGKKFKLRDILSPDMISPPLGDFRHTIHIGKEGQHDVFGDISFLQ : .:.::::: .. .:::: ::::.:: :::::::::::::::::. : :.:::::::: NP_006 MSTKVPIYLKRGS-RKGKKEKLRDLLSSDMISPPLGDFRHTIHIGSGGGSDMFGDISFLQ 10 20 30 40 50 70 80 90 100 110 pF1KB8 GNYELLPGNQEKA--HLGQFPGHNEFFRANSTSDSVFTETPSPVLKNAISLPTIGGSQAL :...::::.. .. . : : .: :. .. . . :::.::::::::.::: ::: NP_006 GKFHLLPGTMVEGPEEDGTFDLPFQFTRTATVCGRELPDGPSPLLKNAISLPVIGGPQAL 60 70 80 90 100 110 120 130 140 150 160 170 pF1KB8 MLPLLSPVTFNSKQESFGPAKLPRLSCEPVMEEKAQEKSSLLENGTVHQGDTSWGSSGSA :: . : .: : ::: : . : :.:.: : : .. NP_006 TLP--------TAQ---APPKPPRLHLET-------PQPSPQEGGSV---DI-WRIPETG 120 130 140 150 180 190 200 210 220 230 pF1KB8 SQSSQGRDSHSSSLSEQYPDWPAEDMFDHPTPCELIKGKTKSEESLSDLTGSLLSLQLDL : .: .:. :. ::. : ::. ..:::::..:: NP_006 SPNS--------GLT---PESGAEEPF------------------LSN-ASSLLSLHVDL 160 170 180 240 250 pF1KB8 GPSLLDEVLNVMDKNK :::.::.::..::.. NP_006 GPSILDDVLQIMDQDLDSMQIPT 190 200 210 >>XP_016872583 (OMIM: 606132) PREDICTED: cdc42 effector (210 aa) initn: 540 init1: 293 opt: 444 Z-score: 447.4 bits: 90.1 E(85289): 3.5e-18 Smith-Waterman score: 493; 43.1% identity (61.6% similar) in 255 aa overlap (1-253:1-202) 10 20 30 40 50 60 pF1KB8 MPAKTPIYLKAANNKKGKKFKLRDILSPDMISPPLGDFRHTIHIGKEGQHDVFGDISFLQ : .:.::::: .. .:::: ::::.:: :::::::::::::::::. : :.:::::::: XP_016 MSTKVPIYLKRGS-RKGKKEKLRDLLSSDMISPPLGDFRHTIHIGSGGGSDMFGDISFLQ 10 20 30 40 50 70 80 90 100 110 pF1KB8 GNYELLPGNQEKA--HLGQFPGHNEFFRANSTSDSVFTETPSPVLKNAISLPTIGGSQAL :...::::.. .. . : : .: :. .. . . :::.::::::::.::: ::: XP_016 GKFHLLPGTMVEGPEEDGTFDLPFQFTRTATVCGRELPDGPSPLLKNAISLPVIGGPQAL 60 70 80 90 100 110 120 130 140 150 160 170 pF1KB8 MLPLLSPVTFNSKQESFGPAKLPRLSCEPVMEEKAQEKSSLLENGTVHQGDTSWGSSGSA :: . : .: : ::: : . : :.:.: : : .. XP_016 TLP--------TAQ---APPKPPRLHLET-------PQPSPQEGGSV---DI-WRIPETG 120 130 140 150 180 190 200 210 220 230 pF1KB8 SQSSQGRDSHSSSLSEQYPDWPAEDMFDHPTPCELIKGKTKSEESLSDLTGSLLSLQLDL : .: .:. :. ::. : ::. ..:::::..:: XP_016 SPNS--------GLT---PESGAEEPF------------------LSN-ASSLLSLHVDL 160 170 180 240 250 pF1KB8 GPSLLDEVLNVMDKNK :::.::.::..::.. XP_016 GPSILDDVLQIMDQDLDSMQIPT 190 200 210 >>NP_689449 (OMIM: 606084) cdc42 effector protein 1 [Hom (391 aa) initn: 274 init1: 116 opt: 200 Z-score: 205.5 bits: 46.2 E(85289): 0.0001 Smith-Waterman score: 214; 31.0% identity (52.2% similar) in 226 aa overlap (26-250:33-214) 10 20 30 40 50 pF1KB8 MPAKTPIYLKAANNKKGKKFKLRDILSPDMISPPLGDFRHTIHIGKEGQHDVFGD :. :::: ::::::::.:.:. : ::::: NP_689 GPQGGRGAATMSLGKLSPVGWVSSSQGKRRLTADMISHPLGDFRHTMHVGRGG--DVFGD 10 20 30 40 50 60 60 70 80 90 100 110 pF1KB8 ISFLQGNYELLPGNQEKAHLGQFPGHNEFFRANSTSDSVFTETPSPVLKNAISLPTIGGS :::. :. :. ... . . . .. : .. .. :.: :. NP_689 TSFLS-NHGGSSGSTHRSPRSFLAKKLQLVRRVGAPPRRMASPPAPS-------PAP--- 70 80 90 100 120 130 140 150 160 170 pF1KB8 QALMLPLLSPVTFNSKQESFGPAKLPRLSCEPVMEEKAQEKSSLLENGTVHQGDTSWGSS : .::. :. .::.:. : . : : . . :.: .: NP_689 -----PAISPIIKNA-------ISLPQLN---------QAAYDSLVVGKL-SFDSS-PTS 110 120 130 140 180 190 200 210 220 230 pF1KB8 GSASQSSQGRDSHSSSLSE-QYPDWPAEDMFDHPTPCELIKGKTKSEESLSDLTGSLLSL .. ..:: : :: ..:. . : . : : : : .:. :: . : NP_689 STDGHSSYGLDSGFCTISRLPRSEKPHDRDRDGSFPSE--PGLRRSDSLLS------FRL 150 160 170 180 190 240 250 pF1KB8 QLDLGPSLLDEVLNVMDKNK .::::::::.:.:.:: NP_689 DLDLGPSLLSELLGVMSLPEAPAAETPAPAANPPAPTANPTGPAANPPATTANPPAPAAN 200 210 220 230 240 250 >>XP_006724172 (OMIM: 606084) PREDICTED: cdc42 effector (391 aa) initn: 274 init1: 116 opt: 200 Z-score: 205.5 bits: 46.2 E(85289): 0.0001 Smith-Waterman score: 214; 31.0% identity (52.2% similar) in 226 aa overlap (26-250:33-214) 10 20 30 40 50 pF1KB8 MPAKTPIYLKAANNKKGKKFKLRDILSPDMISPPLGDFRHTIHIGKEGQHDVFGD :. :::: ::::::::.:.:. : ::::: XP_006 GPQGGRGAATMSLGKLSPVGWVSSSQGKRRLTADMISHPLGDFRHTMHVGRGG--DVFGD 10 20 30 40 50 60 60 70 80 90 100 110 pF1KB8 ISFLQGNYELLPGNQEKAHLGQFPGHNEFFRANSTSDSVFTETPSPVLKNAISLPTIGGS :::. :. :. ... . . . .. : .. .. :.: :. XP_006 TSFLS-NHGGSSGSTHRSPRSFLAKKLQLVRRVGAPPRRMASPPAPS-------PAP--- 70 80 90 100 120 130 140 150 160 170 pF1KB8 QALMLPLLSPVTFNSKQESFGPAKLPRLSCEPVMEEKAQEKSSLLENGTVHQGDTSWGSS : .::. :. .::.:. : . : : . . :.: .: XP_006 -----PAISPIIKNA-------ISLPQLN---------QAAYDSLVVGKL-SFDSS-PTS 110 120 130 140 180 190 200 210 220 230 pF1KB8 GSASQSSQGRDSHSSSLSE-QYPDWPAEDMFDHPTPCELIKGKTKSEESLSDLTGSLLSL .. ..:: : :: ..:. . : . : : : : .:. :: . : XP_006 STDGHSSYGLDSGFCTISRLPRSEKPHDRDRDGSFPSE--PGLRRSDSLLS------FRL 150 160 170 180 190 240 250 pF1KB8 QLDLGPSLLDEVLNVMDKNK .::::::::.:.:.:: XP_006 DLDLGPSLLSELLGVMSLPEAPAAETPAPAANPPAPTANPTGPAANPPATTANPPAPAAN 200 210 220 230 240 250 >>XP_006724170 (OMIM: 606084) PREDICTED: cdc42 effector (391 aa) initn: 274 init1: 116 opt: 200 Z-score: 205.5 bits: 46.2 E(85289): 0.0001 Smith-Waterman score: 214; 31.0% identity (52.2% similar) in 226 aa overlap (26-250:33-214) 10 20 30 40 50 pF1KB8 MPAKTPIYLKAANNKKGKKFKLRDILSPDMISPPLGDFRHTIHIGKEGQHDVFGD :. :::: ::::::::.:.:. : ::::: XP_006 GPQGGRGAATMSLGKLSPVGWVSSSQGKRRLTADMISHPLGDFRHTMHVGRGG--DVFGD 10 20 30 40 50 60 60 70 80 90 100 110 pF1KB8 ISFLQGNYELLPGNQEKAHLGQFPGHNEFFRANSTSDSVFTETPSPVLKNAISLPTIGGS :::. :. :. ... . . . .. : .. .. :.: :. XP_006 TSFLS-NHGGSSGSTHRSPRSFLAKKLQLVRRVGAPPRRMASPPAPS-------PAP--- 70 80 90 100 120 130 140 150 160 170 pF1KB8 QALMLPLLSPVTFNSKQESFGPAKLPRLSCEPVMEEKAQEKSSLLENGTVHQGDTSWGSS : .::. :. .::.:. : . : : . . :.: .: XP_006 -----PAISPIIKNA-------ISLPQLN---------QAAYDSLVVGKL-SFDSS-PTS 110 120 130 140 180 190 200 210 220 230 pF1KB8 GSASQSSQGRDSHSSSLSE-QYPDWPAEDMFDHPTPCELIKGKTKSEESLSDLTGSLLSL .. ..:: : :: ..:. . : . : : : : .:. :: . : XP_006 STDGHSSYGLDSGFCTISRLPRSEKPHDRDRDGSFPSE--PGLRRSDSLLS------FRL 150 160 170 180 190 240 250 pF1KB8 QLDLGPSLLDEVLNVMDKNK .::::::::.:.:.:: XP_006 DLDLGPSLLSELLGVMSLPEAPAAETPAPAANPPAPTANPTGPAANPPATTANPPAPAAN 200 210 220 230 240 250 254 residues in 1 query sequences 60827320 residues in 85289 library sequences Tcomplib [36.3.4 Apr, 2011] (8 proc) start: Sat Nov 5 01:34:37 2016 done: Sat Nov 5 01:34:38 2016 Total Scan time: 7.720 Total Display time: -0.010 Function used was FASTA [36.3.4 Apr, 2011]