Restriction map for
pF1KB8597
(Circular) (Six-base) MAPSORT of: pF1KB8597.seq Check: 4989 from: 1 to: 3875
pF1KB8597 3875 bp
With 182 enzymes: SgfI *
August 7, 2008 14:13 ..
AccI GT'mk_AC
Cuts at: 913 913
Size: 3875
Acc65I G'GTAC_C
Cuts at: 891 891
Size: 3875
AclI AA'CG_TT
Cuts at: 3715 3715
Size: 3875
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1700 2132 2485 3147 1700
Size: 432 353 662 2428
Fragments arranged by size:
2428 662 432 353
AflIII A'CryG_T
Cuts at: 716 1227 3027 3308 716
Size: 511 1800 281 1283
Fragments arranged by size:
1800 1283 511 281
AhdI GACnn_n'nnGTC
Cuts at: 183 183
Size: 3875
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 373 405 373
Size: 32 3843
AlwNI CAG_nnn'CTG
Cuts at: 1180 2618 1180
Size: 1438 2437
ApaI G_GGCC'C
Cuts at: 827 827
Size: 3875
ApaLI G'TGCA_C
Cuts at: 2713 2713
Size: 3875
ApoI r'AATT_y
Cuts at: 163 879 3228 3484 163
Size: 716 2349 256 554
Fragments arranged by size:
2349 716 554 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3875
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3875
AvaI C'yCGr_G
Cuts at: 895 895
Size: 3875
BamHI G'GATC_C
Cuts at: 448 900 448
Size: 452 3423
BanI G'GyrC_C
Cuts at: 891 1305 1553 1588 891
Size: 414 248 35 3178
Fragments arranged by size:
3178 414 248 35
BanII G_rGCy'C
Cuts at: 548 628 746 827 889 1919 548
Size: 80 118 81 62 1030 2504
Fragments arranged by size:
2504 1030 118 81 80 62
BbeI G_GCGC'C
Cuts at: 1309 1557 1309
Size: 248 3627
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3841
BciVI GTATCCnnnnn_n'
Cuts at: 1773 2829 3875 1773
Size: 1056 1046 1773
Fragments arranged by size:
1773 1056 1046
BclI T'GATC_A
Cuts at: 197 1395 197
Size: 1198 2677
BglII A'GATC_T
Cuts at: 1390 3427 1390
Size: 2037 1838
BlpI GC'TnA_GC
Cuts at: 535 987 535
Size: 452 3423
Bme1580I G_kGCm'C
Cuts at: 827 1500 1593 2717 827
Size: 673 93 1124 1985
Fragments arranged by size:
1985 1124 673 93
BmrI ACTGGGnnnn_n'
Cuts at: 1087 1168 1503 3647 1087
Size: 81 335 2144 1315
Fragments arranged by size:
2144 1315 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 795 2242 795
Size: 1447 2428
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 612 660 612
Size: 48 3827
Bpu10I CC'TnA_GC
Cuts at: 566 566
Size: 3875
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1051 1930 2644 2942 1051
Size: 879 714 298 1984
Fragments arranged by size:
1984 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 3579 3579
Size: 3875
BsaAI yAC'GTr
Cuts at: 1228 1228
Size: 3875
BsaBI GATnn'nnATC
Cuts at: 6 1389 3426 6
Size: 1383 2037 455
Fragments arranged by size:
2037 1383 455
BsaHI Gr'CG_yC
Cuts at: 1306 1554 2256 3752 1306
Size: 248 702 1496 1429
Fragments arranged by size:
1496 1429 702 248
BsaWI w'CCGG_w
Cuts at: 1064 1585 2263 2674 2821 3095 1064
Size: 521 678 411 147 274 1844
Fragments arranged by size:
1844 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 655 685 655
Size: 30 3845
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 663 784 790 663
Size: 121 6 3748
Fragments arranged by size:
3748 121 6
BseYI C'CCAG_C
Cuts at: 1300 2058 2723 3131 1300
Size: 758 665 408 2044
Fragments arranged by size:
2044 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 1417 1463 2693 104
Size: 1313 46 1230 1286
Fragments arranged by size:
1313 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 416 746 889 1667 1857 2717 416
Size: 330 143 778 190 860 1574
Fragments arranged by size:
1574 860 778 330 190 143
Bsp1286I G_dGCh'C
Cuts at: 416 548 628 746 827 889 1500 1593 1667
Size: 132 80 118 81 62 611 93 74
Cuts at: 1667 1857 1919 2717 416
Size: 190 62 798 1574
Fragments arranged by size:
1574 798 611 190 132 118 93 81
80 74 62 62
BspHI T'CATG_A
Cuts at: 2307 2307
Size: 3875
BspMI ACCTGCnnnn'nnnn_
Cuts at: 925 1139 1441 1822 925
Size: 214 302 381 2978
Fragments arranged by size:
2978 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2167 2221 3705 3870 50
Size: 2117 54 1484 165 55
Fragments arranged by size:
2117 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1787 1787
Size: 3875
BsrFI r'CCGG_y
Cuts at: 1873 2054 1873
Size: 181 3694
BssSI C'ACGA_G
Cuts at: 618 2146 2854 618
Size: 1528 708 1639
Fragments arranged by size:
1639 1528 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1252 1252
Size: 3875
BstBI TT'CG_AA
Cuts at: 2236 2236
Size: 3875
BstXI CCAn_nnnn'nTGG
Cuts at: 343 343
Size: 3875
BstYI r'GATC_y
Cuts at: 1 448 900 1367 1390 1725 1971 2277 2289
Size: 447 452 467 23 335 246 306 12
Cuts at: 2289 2375 3427 1
Size: 86 1052 449
Fragments arranged by size:
1052 467 452 449 447 335 306 246
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1825 2006 2169 1825
Size: 181 163 3531
Fragments arranged by size:
3531 181 163
BtsI GCAGTG_nn'
Cuts at: 1118 1118
Size: 3875
DraI TTT'AAA
Cuts at: 168 874 168
Size: 706 3169
DraIII CAC_nnn'GTG
Cuts at: 620 620
Size: 3875
DrdI GACnn_nn'nnGTC
Cuts at: 1581 2925 1581
Size: 1344 2531
EaeI y'GGCC_r
Cuts at: 703 1460 1634 2025 2052 3397 703
Size: 757 174 391 27 1345 1181
Fragments arranged by size:
1345 1181 757 391 174 27
EagI C'GGCC_G
Cuts at: 1460 1460
Size: 3875
EarI CTCTTCn'nnn_
Cuts at: 1898 2108 1898
Size: 210 3665
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2083 2825 2971 3685 2083
Size: 742 146 714 2273
Fragments arranged by size:
2273 742 714 146
EcoICRI GAG'CTC
Cuts at: 744 887 744
Size: 143 3732
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 612 660 1700 2132 2485 3147 612
Size: 48 1040 432 353 662 1340
Fragments arranged by size:
1340 1040 662 432 353 48
EcoO109I rG'GnC_Cy
Cuts at: 824 1014 824
Size: 190 3685
EcoRI G'AATT_C
Cuts at: 879 879
Size: 3875
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1894 1894
Size: 3875
FspI TGC'GCA
Cuts at: 1656 1656
Size: 3875
HaeII r_GCGC'y
Cuts at: 1309 1557 2787 3558 1309
Size: 248 1230 771 1626
Fragments arranged by size:
1626 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 169 201 655 687 1717 1747 1749 1779 169
Size: 32 454 32 1030 30 2 30 2265
Fragments arranged by size:
2265 1030 454 32 32 30 30 2
HincII GTy'rAC
Cuts at: 914 914
Size: 3875
KasI G'GCGC_C
Cuts at: 1305 1553 1305
Size: 248 3627
KpnI G_GTAC'C
Cuts at: 895 895
Size: 3875
MluI A'CGCG_T
Cuts at: 3308 3308
Size: 3875
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 566 2634 2818 566
Size: 2068 184 1623
Fragments arranged by size:
2068 1623 184
MscI TGG'CCA
Cuts at: 705 1636 705
Size: 931 2944
MslI CAynn'nnrTG
Cuts at: 1864 1991 1864
Size: 127 3748
MspA1I CmG'CkG
Cuts at: 632 986 1300 1660 2444 2689 632
Size: 354 314 360 784 245 1818
Fragments arranged by size:
1818 784 360 354 314 245
NaeI GCC'GGC
Cuts at: 2056 2056
Size: 3875
NarI GG'CG_CC
Cuts at: 1306 1554 1306
Size: 248 3627
NgoMIV G'CCGG_C
Cuts at: 2054 2054
Size: 3875
NspI r_CATG'y
Cuts at: 720 928 1428 3031 720
Size: 208 500 1603 1564
Fragments arranged by size:
1603 1564 500 208
PasI CC'CwG_GG
Cuts at: 343 343
Size: 3875
PciI A'CATG_T
Cuts at: 716 3027 716
Size: 2311 1564
PmeI GTTT'AAAC
Cuts at: 874 874
Size: 3875
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2321 2353 2321
Size: 32 3843
PspOMI G'GGCC_C
Cuts at: 823 823
Size: 3875
PstI C_TGCA'G
Cuts at: 812 922 1607 812
Size: 110 685 3080
Fragments arranged by size:
3080 685 110
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3875
PvuII CAG'CTG
Cuts at: 1300 1660 1300
Size: 360 3515
RsrII CG'GwC_CG
Cuts at: 2070 2070
Size: 3875
SacI G_AGCT'C
Cuts at: 746 889 746
Size: 143 3732
SalI G'TCGA_C
Cuts at: 912 912
Size: 3875
SapI GCTCTTCn'nnn_
Cuts at: 1898 2108 1898
Size: 210 3665
SbfI CC_TGCA'GG
Cuts at: 922 922
Size: 3875
ScaI AGT'ACT
Cuts at: 3392 3392
Size: 3875
SfcI C'TryA_G
Cuts at: 33 808 918 1603 2571 2762 33
Size: 775 110 685 968 191 1146
Fragments arranged by size:
1146 968 775 685 191 110
SfoI GGC'GCC
Cuts at: 1307 1555 1307
Size: 248 3627
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3875
SmaI CCC'GGG
Cuts at: 897 897
Size: 3875
SmlI C'TyrA_G
Cuts at: 1030 1945 2659 2921 1030
Size: 915 714 262 1984
Fragments arranged by size:
1984 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3875
SphI G_CATG'C
Cuts at: 928 1428 928
Size: 500 3375
StyI C'CwwG_G
Cuts at: 654 1009 1363 654
Size: 355 354 3166
Fragments arranged by size:
3166 355 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1458 1591 2050 2259 1458
Size: 133 459 209 3074
Fragments arranged by size:
3074 459 209 133
TatI w'GTAC_w
Cuts at: 3390 3390
Size: 3875
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3392 3424 3392
Size: 32 3843
Tth111I GACn'n_nGTC
Cuts at: 762 1672 762
Size: 910 2965
XbaI T'CTAG_A
Cuts at: 906 906
Size: 3875
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1370 1370
Size: 3875
XmaI C'CCGG_G
Cuts at: 895 895
Size: 3875
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AhdI AloI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BcgI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PasI
PciI PmeI PpiI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AleI AlfI AscI
AvrII BaeI BbsI BbvCI BfrBI BglI BmgBI BmtI
BsgI BsiWI BsmI BsmBI BspEI BsrGI BssHII BstEII
BstZ17I Bsu36I BtgI ClaI CspCI EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI
NruI NsiI PacI PflMI PfoI PmlI PpuMI PshAI
PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI