Restriction map for
pF1KB8598
(Circular) (Six-base) MAPSORT of: pF1KB8598.seq Check: 5353 from: 1 to: 5441
pF1KB8598 5441 bp
With 182 enzymes: SgfI *
March 2, 2010 14:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 186 186
Size: 5441
AccI GT'mk_AC
Cuts at: 1918 2479 1918
Size: 561 4880
Acc65I G'GTAC_C
Cuts at: 2457 2457
Size: 5441
AclI AA'CG_TT
Cuts at: 5281 5281
Size: 5441
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 163 1603 1927 3266 3698 4445 4713 163
Size: 1440 324 1339 432 747 268 891
Fragments arranged by size:
1440 1339 891 747 432 324 268
AflII C'TTAA_G
Cuts at: 382 418 382
Size: 36 5405
AflIII A'CryG_T
Cuts at: 2793 3897 4874 2793
Size: 1104 977 3360
Fragments arranged by size:
3360 1104 977
AgeI A'CCGG_T
Cuts at: 3829 3829
Size: 5441
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1738 1738
Size: 5441
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 290 322 290
Size: 32 5409
AlwNI CAG_nnn'CTG
Cuts at: 880 1980 2746 4313 880
Size: 1100 766 1567 2008
Fragments arranged by size:
2008 1567 1100 766
ApaI G_GGCC'C
Cuts at: 775 775
Size: 5441
ApaLI G'TGCA_C
Cuts at: 4211 4211
Size: 5441
ApoI r'AATT_y
Cuts at: 388 457 512 856 1010 1024 1032 2445 4794
Size: 69 55 344 154 14 8 1413 2349
Cuts at: 4794 5050 388
Size: 256 779
Fragments arranged by size:
2349 1413 779 344 256 154 69 55
14 8
AseI AT'TA_AT
Cuts at: 20 20
Size: 5441
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5441
AvaI C'yCGr_G
Cuts at: 2339 2461 2339
Size: 122 5319
AvrII C'CTAG_G
Cuts at: 1104 1104
Size: 5441
BamHI G'GATC_C
Cuts at: 2466 2466
Size: 5441
BanI G'GyrC_C
Cuts at: 2457 2871 3119 3154 2457
Size: 414 248 35 4744
Fragments arranged by size:
4744 414 248 35
BanII G_rGCy'C
Cuts at: 242 351 775 1732 2455 3485 242
Size: 109 424 957 723 1030 2198
Fragments arranged by size:
2198 1030 957 723 424 109
BbeI G_GCGC'C
Cuts at: 2875 3123 2875
Size: 248 5193
BbsI GAAGACnn'nnnn_
Cuts at: 1442 1455 1484 2228 1442
Size: 13 29 744 4655
Fragments arranged by size:
4655 744 29 13
BciVI GTATCCnnnnn_n'
Cuts at: 3339 4100 5441 3339
Size: 761 1341 3339
Fragments arranged by size:
3339 1341 761
BclI T'GATC_A
Cuts at: 2351 2961 2351
Size: 610 4831
BfrBI ATG'CAT
Cuts at: 760 2363 760
Size: 1603 3838
BglI GCCn_nnn'nGGC
Cuts at: 1832 1832
Size: 5441
BglII A'GATC_T
Cuts at: 164 1653 2956 4993 164
Size: 1489 1303 2037 612
Fragments arranged by size:
2037 1489 1303 612
BlpI GC'TnA_GC
Cuts at: 1726 2553 1726
Size: 827 4614
Bme1580I G_kGCm'C
Cuts at: 775 3066 3159 4215 775
Size: 2291 93 1056 2001
Fragments arranged by size:
2291 2001 1056 93
BmgBI CAC'GTC
Cuts at: 1847 1847
Size: 5441
BmrI ACTGGGnnnn_n'
Cuts at: 868 2653 2734 3069 5213 868
Size: 1785 81 335 2144 1096
Fragments arranged by size:
2144 1785 1096 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1626 3808 1626
Size: 2182 3259
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 323 362 2310 323
Size: 39 1948 3454
Fragments arranged by size:
3454 1948 39
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 934 2617 3496 3988 4286 934
Size: 1683 879 492 298 2089
Fragments arranged by size:
2089 1683 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 5145 5145
Size: 5441
BsaAI yAC'GTr
Cuts at: 732 2794 732
Size: 2062 3379
BsaBI GATnn'nnATC
Cuts at: 6 1933 2955 4992 6
Size: 1927 1022 2037 455
Fragments arranged by size:
2037 1927 1022 455
BsaHI Gr'CG_yC
Cuts at: 1915 2872 3120 3822 5318 1915
Size: 957 248 702 1496 2038
Fragments arranged by size:
2038 1496 957 702 248
BsaWI w'CCGG_w
Cuts at: 2630 3151 3829 4103 4250 4661 2630
Size: 521 678 274 147 411 3410
Fragments arranged by size:
3410 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 290 320 600 630 1372 1402 1862 1892 290
Size: 30 280 30 742 30 460 30 3839
Fragments arranged by size:
3839 742 460 280 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 311 359 1435 311
Size: 48 1076 4317
Fragments arranged by size:
4317 1076 48
BseYI C'CCAG_C
Cuts at: 2866 3624 4201 4697 2866
Size: 758 577 496 3610
Fragments arranged by size:
3610 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1993 1993
Size: 5441
BsiEI CG_ry'CG
Cuts at: 104 2983 3029 4237 104
Size: 2879 46 1208 1308
Fragments arranged by size:
2879 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 242 351 1732 2455 3233 3423 4215 242
Size: 109 1381 723 778 190 792 1468
Fragments arranged by size:
1468 1381 792 778 723 190 109
BsmI GAATG_Cn'
Cuts at: 1258 1715 1258
Size: 457 4984
Bsp1286I G_dGCh'C
Cuts at: 242 351 775 1732 2455 3066 3159 3233 3423
Size: 109 424 957 723 611 93 74 190
Cuts at: 3423 3485 4215 242
Size: 62 730 1468
Fragments arranged by size:
1468 957 730 723 611 424 190 109
93 74 62
BspEI T'CCGG_A
Cuts at: 4661 4661
Size: 5441
BspHI T'CATG_A
Cuts at: 945 2261 4617 945
Size: 1316 2356 1769
Fragments arranged by size:
2356 1769 1316
BspMI ACCTGCnnnn'nnnn_
Cuts at: 186 2491 2705 3007 3388 186
Size: 2305 214 302 381 2239
Fragments arranged by size:
2305 2239 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3733 3787 5271 5436 50
Size: 3683 54 1484 165 55
Fragments arranged by size:
3683 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3353 3353
Size: 5441
BsrFI r'CCGG_y
Cuts at: 184 3439 3620 3829 184
Size: 3255 181 209 1796
Fragments arranged by size:
3255 1796 209 181
BsrGI T'GTAC_A
Cuts at: 200 720 1206 1759 200
Size: 520 486 553 3882
Fragments arranged by size:
3882 553 520 486
BssSI C'ACGA_G
Cuts at: 293 3712 4070 293
Size: 3419 358 1664
Fragments arranged by size:
3419 1664 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2818 2818
Size: 5441
BstBI TT'CG_AA
Cuts at: 3802 3802
Size: 5441
BstEII G'GTnAC_C
Cuts at: 1329 1329
Size: 5441
BstXI CCAn_nnnn'nTGG
Cuts at: 234 234
Size: 5441
BstYI r'GATC_y
Cuts at: 1 164 1653 2466 2933 2956 3291 3537 4549
Size: 163 1489 813 467 23 335 246 1012
Cuts at: 4549 4635 4647 4993 1
Size: 86 12 346 449
Fragments arranged by size:
1489 1012 813 467 449 346 335 246
163 86 23 12
BtgI C'CryG_G
Cuts at: 135 135
Size: 5441
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3391 3572 3735 3391
Size: 181 163 5097
Fragments arranged by size:
5097 181 163
BtsI GCAGTG_nn'
Cuts at: 2684 2684
Size: 5441
ClaI AT'CG_AT
Cuts at: 486 486
Size: 5441
DraI TTT'AAA
Cuts at: 659 1187 2440 659
Size: 528 1253 3660
Fragments arranged by size:
3660 1253 528
DrdI GACnn_nn'nnGTC
Cuts at: 3147 4005 3147
Size: 858 4583
EaeI y'GGCC_r
Cuts at: 218 1833 2246 3026 3200 3591 3618 4963 218
Size: 1615 413 780 174 391 27 1345 696
Fragments arranged by size:
1615 1345 780 696 413 391 174 27
EagI C'GGCC_G
Cuts at: 3026 3026
Size: 5441
EarI CTCTTCn'nnn_
Cuts at: 921 3464 3674 921
Size: 2543 210 2688
Fragments arranged by size:
2688 2543 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3649 3959 4105 5251 3649
Size: 310 146 1146 3839
Fragments arranged by size:
3839 1146 310 146
EcoICRI GAG'CTC
Cuts at: 240 349 1730 2453 240
Size: 109 1381 723 3228
Fragments arranged by size:
3228 1381 723 109
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 163 323 362 1603 1927 2310 3266 3698 4445
Size: 160 39 1241 324 383 956 432 747
Cuts at: 4445 4713 163
Size: 268 891
Fragments arranged by size:
1241 956 891 747 432 383 324 268
160 39
EcoO109I rG'GnC_Cy
Cuts at: 771 772 1736 2307 2580 771
Size: 1 964 571 273 3632
Fragments arranged by size:
3632 964 571 273 1
EcoRI G'AATT_C
Cuts at: 856 2445 856
Size: 1589 3852
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 420 3460 420
Size: 3040 2401
FspI TGC'GCA
Cuts at: 3222 3222
Size: 5441
HaeII r_GCGC'y
Cuts at: 2875 3123 4145 5124 2875
Size: 248 1022 979 3192
Fragments arranged by size:
3192 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 173 205 600 632 1638 1670 3283 3313 3315
Size: 32 395 32 1006 32 1613 30 2
Cuts at: 3315 3345 173
Size: 30 2269
Fragments arranged by size:
2269 1613 1006 395 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 116 782 1919 2154 2480 116
Size: 666 1137 235 326 3077
Fragments arranged by size:
3077 1137 666 326 235
HindIII A'AGCT_T
Cuts at: 1589 1589
Size: 5441
HpaI GTT'AAC
Cuts at: 782 2154 782
Size: 1372 4069
KasI G'GCGC_C
Cuts at: 2871 3119 2871
Size: 248 5193
KpnI G_GTAC'C
Cuts at: 2461 2461
Size: 5441
MluI A'CGCG_T
Cuts at: 4874 4874
Size: 5441
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1106 1763 4112 4296 1106
Size: 657 2349 184 2251
Fragments arranged by size:
2349 2251 657 184
MscI TGG'CCA
Cuts at: 220 3202 220
Size: 2982 2459
MslI CAynn'nnrTG
Cuts at: 1642 1720 2161 2266 3430 3557 1642
Size: 78 441 105 1164 127 3526
Fragments arranged by size:
3526 1164 441 127 105 78
MspA1I CmG'CkG
Cuts at: 1980 2319 2552 2866 3226 4239 4484 1980
Size: 339 233 314 360 1013 245 2937
Fragments arranged by size:
2937 1013 360 339 314 245 233
NaeI GCC'GGC
Cuts at: 186 3622 186
Size: 3436 2005
NarI GG'CG_CC
Cuts at: 2872 3120 2872
Size: 248 5193
NgoMIV G'CCGG_C
Cuts at: 184 3620 184
Size: 3436 2005
NsiI A_TGCA'T
Cuts at: 762 2365 762
Size: 1603 3838
NspI r_CATG'y
Cuts at: 1074 1629 2494 2994 3901 1074
Size: 555 865 500 907 2614
Fragments arranged by size:
2614 907 865 555 500
PciI A'CATG_T
Cuts at: 3897 3897
Size: 5441
PfoI T'CCnGG_A
Cuts at: 1094 1094
Size: 5441
PmeI GTTT'AAAC
Cuts at: 2440 2440
Size: 5441
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 290 322 974 1006 4580 4612 290
Size: 32 652 32 3574 32 1119
Fragments arranged by size:
3574 1119 652 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 1736 2307 1736
Size: 571 4870
PsiI TTA'TAA
Cuts at: 257 2132 257
Size: 1875 3566
PspOMI G'GGCC_C
Cuts at: 771 771
Size: 5441
PspXI vC'TCGA_Gb
Cuts at: 2339 2339
Size: 5441
PstI C_TGCA'G
Cuts at: 2488 3173 2488
Size: 685 4756
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5441
PvuII CAG'CTG
Cuts at: 1980 2319 2866 3226 1980
Size: 339 547 360 4195
Fragments arranged by size:
4195 547 360 339
RsrII CG'GwC_CG
Cuts at: 3636 3636
Size: 5441
SacI G_AGCT'C
Cuts at: 242 351 1732 2455 242
Size: 109 1381 723 3228
Fragments arranged by size:
3228 1381 723 109
SalI G'TCGA_C
Cuts at: 1917 2478 1917
Size: 561 4880
SapI GCTCTTCn'nnn_
Cuts at: 3464 3674 3464
Size: 210 5231
SbfI CC_TGCA'GG
Cuts at: 2488 2488
Size: 5441
ScaI AGT'ACT
Cuts at: 852 4958 852
Size: 4106 1335
SfcI C'TryA_G
Cuts at: 33 2421 2484 3169 4162 4353 33
Size: 2388 63 685 993 191 1121
Fragments arranged by size:
2388 1121 993 685 191 63
SfoI GGC'GCC
Cuts at: 2873 3121 2873
Size: 248 5193
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5441
SmaI CCC'GGG
Cuts at: 2463 2463
Size: 5441
SmlI C'TyrA_G
Cuts at: 382 418 913 2339 2596 3511 4003 4265 382
Size: 36 495 1426 257 915 492 262 1558
Fragments arranged by size:
1558 1426 915 495 492 262 257 36
SpeI A'CTAG_T
Cuts at: 67 268 67
Size: 201 5240
SphI G_CATG'C
Cuts at: 2494 2994 2494
Size: 500 4941
SspI AAT'ATT
Cuts at: 2389 2389
Size: 5441
StyI C'CwwG_G
Cuts at: 1104 2575 2929 1104
Size: 1471 354 3616
Fragments arranged by size:
3616 1471 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1999 3024 3157 3616 3825 1999
Size: 1025 133 459 209 3615
Fragments arranged by size:
3615 1025 459 209 133
TatI w'GTAC_w
Cuts at: 200 265 450 720 850 1206 1469 1604 1759
Size: 65 185 270 130 356 263 135 155
Cuts at: 1759 1946 4956 200
Size: 187 3010 685
Fragments arranged by size:
3010 685 356 270 263 187 185 155
135 130 65
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4958 4990 4958
Size: 32 5409
Tth111I GACn'n_nGTC
Cuts at: 3238 3238
Size: 5441
XbaI T'CTAG_A
Cuts at: 1087 2472 1087
Size: 1385 4056
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2296 2936 2296
Size: 640 4801
XhoI C'TCGA_G
Cuts at: 2339 2339
Size: 5441
XmaI C'CCGG_G
Cuts at: 2461 2461
Size: 5441
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BbsI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NsiI NspI PciI PfoI
PmeI PpiI PpuMI PsiI PspOMI PspXI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AfeI AhdI AleI AscI BaeI BbvCI BcgI
BmtI Bpu10I BsiWI BsmBI BssHII BstZ17I Bsu36I CspCI
DraIII EcoNI EcoRV FseI FspAI MfeI NcoI NdeI
NheI NotI NruI PacI PasI PflMI PmlI PshAI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
StuI SwaI XmnI ZraI