Restriction map for
pF1KB8601
(Circular) (Six-base) MAPSORT of: pF1KB8601.seq Check: 447 from: 1 to: 3617
pF1KB8601 3617 bp
With 182 enzymes: SgfI *
March 2, 2010 14:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 144 144
Size: 3617
AccI GT'mk_AC
Cuts at: 655 655
Size: 3617
Acc65I G'GTAC_C
Cuts at: 633 633
Size: 3617
AclI AA'CG_TT
Cuts at: 3457 3457
Size: 3617
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 361 1442 1874 2227 2889 361
Size: 1081 432 353 662 1089
Fragments arranged by size:
1089 1081 662 432 353
AflIII A'CryG_T
Cuts at: 969 2769 3050 969
Size: 1800 281 1536
Fragments arranged by size:
1800 1536 281
AlwNI CAG_nnn'CTG
Cuts at: 922 2360 922
Size: 1438 2179
ApaLI G'TGCA_C
Cuts at: 2455 2455
Size: 3617
ApoI r'AATT_y
Cuts at: 325 621 2970 3226 325
Size: 296 2349 256 716
Fragments arranged by size:
2349 716 296 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3617
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3617
AvaI C'yCGr_G
Cuts at: 637 637
Size: 3617
BamHI G'GATC_C
Cuts at: 642 642
Size: 3617
BanI G'GyrC_C
Cuts at: 633 1047 1295 1330 633
Size: 414 248 35 2920
Fragments arranged by size:
2920 414 248 35
BanII G_rGCy'C
Cuts at: 631 1661 631
Size: 1030 2587
BbeI G_GCGC'C
Cuts at: 1051 1299 1051
Size: 248 3369
BciVI GTATCCnnnnn_n'
Cuts at: 1515 2571 3617 1515
Size: 1056 1046 1515
Fragments arranged by size:
1515 1056 1046
BclI T'GATC_A
Cuts at: 471 1137 471
Size: 666 2951
BglII A'GATC_T
Cuts at: 1132 3169 1132
Size: 2037 1580
BlpI GC'TnA_GC
Cuts at: 729 729
Size: 3617
Bme1580I G_kGCm'C
Cuts at: 1242 1335 2459 1242
Size: 93 1124 2400
Fragments arranged by size:
2400 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 829 910 1245 3389 829
Size: 81 335 2144 1057
Fragments arranged by size:
2144 1057 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 244 1984 244
Size: 1740 1877
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 277 481 793 1672 2386 2684 277
Size: 204 312 879 714 298 1210
Fragments arranged by size:
1210 879 714 312 298 204
BsaI GGTCTCn'nnnn_
Cuts at: 3321 3321
Size: 3617
BsaAI yAC'GTr
Cuts at: 970 970
Size: 3617
BsaBI GATnn'nnATC
Cuts at: 6 1131 3168 6
Size: 1125 2037 455
Fragments arranged by size:
2037 1125 455
BsaHI Gr'CG_yC
Cuts at: 1048 1296 1998 3494 1048
Size: 248 702 1496 1171
Fragments arranged by size:
1496 1171 702 248
BsaWI w'CCGG_w
Cuts at: 806 1327 2005 2416 2563 2837 806
Size: 521 678 411 147 274 1586
Fragments arranged by size:
1586 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 579 579
Size: 3617
BseYI C'CCAG_C
Cuts at: 477 1042 1800 2465 2873 477
Size: 565 758 665 408 1221
Fragments arranged by size:
1221 758 665 565 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 219 219
Size: 3617
BsiEI CG_ry'CG
Cuts at: 104 1159 1205 2435 104
Size: 1055 46 1230 1286
Fragments arranged by size:
1286 1230 1055 46
BsiHKAI G_wGCw'C
Cuts at: 631 1409 1599 2459 631
Size: 778 190 860 1789
Fragments arranged by size:
1789 860 778 190
Bsp1286I G_dGCh'C
Cuts at: 631 1242 1335 1409 1599 1661 2459 631
Size: 611 93 74 190 62 798 1789
Fragments arranged by size:
1789 798 611 190 93 74 62
BspHI T'CATG_A
Cuts at: 2049 2049
Size: 3617
BspMI ACCTGCnnnn'nnnn_
Cuts at: 144 667 881 1183 1564 144
Size: 523 214 302 381 2197
Fragments arranged by size:
2197 523 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1909 1963 3447 3612 50
Size: 1859 54 1484 165 55
Fragments arranged by size:
1859 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 252 1529 252
Size: 1277 2340
BsrFI r'CCGG_y
Cuts at: 1615 1796 1615
Size: 181 3436
BssSI C'ACGA_G
Cuts at: 598 1888 2596 598
Size: 1290 708 1619
Fragments arranged by size:
1619 1290 708
BstAPI GCAn_nnn'nTGC
Cuts at: 136 242 487 994 136
Size: 106 245 507 2759
Fragments arranged by size:
2759 507 245 106
BstBI TT'CG_AA
Cuts at: 1978 1978
Size: 3617
BstYI r'GATC_y
Cuts at: 1 642 1109 1132 1467 1713 2019 2031 2117
Size: 641 467 23 335 246 306 12 86
Cuts at: 2117 3169 1
Size: 1052 449
Fragments arranged by size:
1052 641 467 449 335 306 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3617
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1567 1748 1911 1567
Size: 181 163 3273
Fragments arranged by size:
3273 181 163
BtsI GCAGTG_nn'
Cuts at: 860 860
Size: 3617
DraI TTT'AAA
Cuts at: 616 616
Size: 3617
DrdI GACnn_nn'nnGTC
Cuts at: 1323 2667 1323
Size: 1344 2273
EaeI y'GGCC_r
Cuts at: 1202 1376 1767 1794 3139 1202
Size: 174 391 27 1345 1680
Fragments arranged by size:
1680 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1202 1202
Size: 3617
EarI CTCTTCn'nnn_
Cuts at: 235 326 380 1640 1850 235
Size: 91 54 1260 210 2002
Fragments arranged by size:
2002 1260 210 91 54
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1825 2567 2713 3427 1825
Size: 742 146 714 2015
Fragments arranged by size:
2015 742 714 146
EcoICRI GAG'CTC
Cuts at: 629 629
Size: 3617
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 361 1442 1874 2227 2889 361
Size: 1081 432 353 662 1089
Fragments arranged by size:
1089 1081 662 432 353
EcoO109I rG'GnC_Cy
Cuts at: 359 756 359
Size: 397 3220
EcoRI G'AATT_C
Cuts at: 621 621
Size: 3617
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 177 1636 177
Size: 1459 2158
FspI TGC'GCA
Cuts at: 395 1398 395
Size: 1003 2614
HaeII r_GCGC'y
Cuts at: 1051 1299 2529 3300 1051
Size: 248 1230 771 1368
Fragments arranged by size:
1368 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1459 1489 1491 1521 1459
Size: 30 2 30 3555
Fragments arranged by size:
3555 30 30 2
HincII GTy'rAC
Cuts at: 609 656 609
Size: 47 3570
KasI G'GCGC_C
Cuts at: 1047 1295 1047
Size: 248 3369
KpnI G_GTAC'C
Cuts at: 637 637
Size: 3617
MluI A'CGCG_T
Cuts at: 3050 3050
Size: 3617
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2376 2560 2376
Size: 184 3433
MscI TGG'CCA
Cuts at: 1378 1378
Size: 3617
MslI CAynn'nnrTG
Cuts at: 215 1606 1733 215
Size: 1391 127 2099
Fragments arranged by size:
2099 1391 127
MspA1I CmG'CkG
Cuts at: 728 1042 1402 2186 2431 728
Size: 314 360 784 245 1914
Fragments arranged by size:
1914 784 360 314 245
NaeI GCC'GGC
Cuts at: 1798 1798
Size: 3617
NarI GG'CG_CC
Cuts at: 1048 1296 1048
Size: 248 3369
NcoI C'CATG_G
Cuts at: 107 107
Size: 3617
NgoMIV G'CCGG_C
Cuts at: 1796 1796
Size: 3617
NspI r_CATG'y
Cuts at: 670 1170 2773 670
Size: 500 1603 1514
Fragments arranged by size:
1603 1514 500
PciI A'CATG_T
Cuts at: 2769 2769
Size: 3617
PfoI T'CCnGG_A
Cuts at: 419 419
Size: 3617
PmeI GTTT'AAAC
Cuts at: 616 616
Size: 3617
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2063 2095 2063
Size: 32 3585
PstI C_TGCA'G
Cuts at: 664 1349 664
Size: 685 2932
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3617
PvuII CAG'CTG
Cuts at: 1042 1402 1042
Size: 360 3257
RsrII CG'GwC_CG
Cuts at: 1812 1812
Size: 3617
SacI G_AGCT'C
Cuts at: 631 631
Size: 3617
SalI G'TCGA_C
Cuts at: 654 654
Size: 3617
SapI GCTCTTCn'nnn_
Cuts at: 326 1640 1850 326
Size: 1314 210 2093
Fragments arranged by size:
2093 1314 210
SbfI CC_TGCA'GG
Cuts at: 664 664
Size: 3617
ScaI AGT'ACT
Cuts at: 390 3134 390
Size: 2744 873
SfcI C'TryA_G
Cuts at: 33 660 1345 2313 2504 33
Size: 627 685 968 191 1146
Fragments arranged by size:
1146 968 685 627 191
SfoI GGC'GCC
Cuts at: 1049 1297 1049
Size: 248 3369
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3617
SmaI CCC'GGG
Cuts at: 639 639
Size: 3617
SmlI C'TyrA_G
Cuts at: 292 460 772 1687 2401 2663 292
Size: 168 312 915 714 262 1246
Fragments arranged by size:
1246 915 714 312 262 168
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3617
SphI G_CATG'C
Cuts at: 670 1170 670
Size: 500 3117
StyI C'CwwG_G
Cuts at: 107 751 1105 107
Size: 644 354 2619
Fragments arranged by size:
2619 644 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 491 1200 1333 1792 2001 491
Size: 709 133 459 209 2107
Fragments arranged by size:
2107 709 459 209 133
TatI w'GTAC_w
Cuts at: 388 3132 388
Size: 2744 873
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3134 3166 3134
Size: 32 3585
Tth111I GACn'n_nGTC
Cuts at: 1414 1414
Size: 3617
XbaI T'CTAG_A
Cuts at: 648 648
Size: 3617
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 511 1112 511
Size: 601 3016
XmaI C'CCGG_G
Cuts at: 637 637
Size: 3617
XmnI GAAnn'nnTTC
Cuts at: 147 147
Size: 3617
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglII BlpI Bme1580I BmrI BplI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PfoI PmeI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BglI BmgBI BmtI BpmI Bpu10I BsaXI BsiWI BsmI
BsmBI BspEI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI ZraI