Restriction map for
pF1KB8602
(Circular) (Six-base) MAPSORT of: pF1KB8602.seq Check: 4454 from: 1 to: 3749
pF1KB8602 3749 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AccI GT'mk_AC
Cuts at: 787 787
Size: 3749
Acc65I G'GTAC_C
Cuts at: 765 765
Size: 3749
AclI AA'CG_TT
Cuts at: 3589 3589
Size: 3749
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 328 354 466 1574 2006 2359 3021 328
Size: 26 112 1108 432 353 662 1056
Fragments arranged by size:
1108 1056 662 432 353 112 26
AflIII A'CryG_T
Cuts at: 1101 2901 3182 1101
Size: 1800 281 1668
Fragments arranged by size:
1800 1668 281
AleI CACnn'nnGTG
Cuts at: 280 280
Size: 3749
AlwNI CAG_nnn'CTG
Cuts at: 356 444 1054 2492 356
Size: 88 610 1438 1613
Fragments arranged by size:
1613 1438 610 88
ApaI G_GGCC'C
Cuts at: 716 716
Size: 3749
ApaLI G'TGCA_C
Cuts at: 2587 2587
Size: 3749
ApoI r'AATT_y
Cuts at: 753 3102 3358 753
Size: 2349 256 1144
Fragments arranged by size:
2349 1144 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3749
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3749
AvaI C'yCGr_G
Cuts at: 769 769
Size: 3749
BamHI G'GATC_C
Cuts at: 774 774
Size: 3749
BanI G'GyrC_C
Cuts at: 295 376 765 1179 1427 1462 295
Size: 81 389 414 248 35 2582
Fragments arranged by size:
2582 414 389 248 81 35
BanII G_rGCy'C
Cuts at: 716 763 1793 716
Size: 47 1030 2672
Fragments arranged by size:
2672 1030 47
BbeI G_GCGC'C
Cuts at: 1183 1431 1183
Size: 248 3501
BbvCI CC'TCA_GC
Cuts at: 514 514
Size: 3749
BciVI GTATCCnnnnn_n'
Cuts at: 256 1647 2703 3749 256
Size: 1391 1056 1046 256
Fragments arranged by size:
1391 1056 1046 256
BclI T'GATC_A
Cuts at: 1269 1269
Size: 3749
BglI GCCn_nnn'nGGC
Cuts at: 720 720
Size: 3749
BglII A'GATC_T
Cuts at: 317 1264 3301 317
Size: 947 2037 765
Fragments arranged by size:
2037 947 765
BlpI GC'TnA_GC
Cuts at: 434 861 434
Size: 427 3322
Bme1580I G_kGCm'C
Cuts at: 716 1374 1467 2591 716
Size: 658 93 1124 1874
Fragments arranged by size:
1874 1124 658 93
BmrI ACTGGGnnnn_n'
Cuts at: 961 1042 1377 3521 961
Size: 81 335 2144 1189
Fragments arranged by size:
2144 1189 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2116 2116
Size: 3749
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 475 475
Size: 3749
Bpu10I CC'TnA_GC
Cuts at: 514 514
Size: 3749
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 334 412 925 1804 2518 2816 334
Size: 78 513 879 714 298 1267
Fragments arranged by size:
1267 879 714 513 298 78
BsaI GGTCTCn'nnnn_
Cuts at: 3453 3453
Size: 3749
BsaAI yAC'GTr
Cuts at: 225 1102 225
Size: 877 2872
BsaBI GATnn'nnATC
Cuts at: 6 1263 3300 6
Size: 1257 2037 455
Fragments arranged by size:
2037 1257 455
BsaHI Gr'CG_yC
Cuts at: 1180 1428 2130 3626 1180
Size: 248 702 1496 1303
Fragments arranged by size:
1496 1303 702 248
BsaWI w'CCGG_w
Cuts at: 938 1459 2137 2548 2695 2969 938
Size: 521 678 411 147 274 1718
Fragments arranged by size:
1718 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 472 475 679 472
Size: 3 204 3542
Fragments arranged by size:
3542 204 3
BseYI C'CCAG_C
Cuts at: 131 148 1174 1932 2597 3005 131
Size: 17 1026 758 665 408 875
Fragments arranged by size:
1026 875 758 665 408 17
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 451 451
Size: 3749
BsiEI CG_ry'CG
Cuts at: 104 1291 1337 2567 104
Size: 1187 46 1230 1286
Fragments arranged by size:
1286 1230 1187 46
BsiHKAI G_wGCw'C
Cuts at: 763 1541 1731 2591 763
Size: 778 190 860 1921
Fragments arranged by size:
1921 860 778 190
Bsp1286I G_dGCh'C
Cuts at: 716 763 1374 1467 1541 1731 1793 2591 716
Size: 47 611 93 74 190 62 798 1874
Fragments arranged by size:
1874 798 611 190 93 74 62 47
BspHI T'CATG_A
Cuts at: 2181 2181
Size: 3749
BspMI ACCTGCnnnn'nnnn_
Cuts at: 799 1013 1315 1696 799
Size: 214 302 381 2852
Fragments arranged by size:
2852 381 302 214
BsrBI CCG'CTC
Cuts at: 50 525 2041 2095 3579 3744 50
Size: 475 1516 54 1484 165 55
Fragments arranged by size:
1516 1484 475 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1661 1661
Size: 3749
BsrFI r'CCGG_y
Cuts at: 1747 1928 1747
Size: 181 3568
BssSI C'ACGA_G
Cuts at: 2020 2728 2020
Size: 708 3041
BstAPI GCAn_nnn'nTGC
Cuts at: 1126 1126
Size: 3749
BstBI TT'CG_AA
Cuts at: 2110 2110
Size: 3749
BstEII G'GTnAC_C
Cuts at: 531 531
Size: 3749
BstYI r'GATC_y
Cuts at: 1 317 774 1241 1264 1599 1845 2151 2163
Size: 316 457 467 23 335 246 306 12
Cuts at: 2163 2249 3301 1
Size: 86 1052 449
Fragments arranged by size:
1052 467 457 449 335 316 306 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 194 107
Size: 87 3662
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1699 1880 2043 1699
Size: 181 163 3405
Fragments arranged by size:
3405 181 163
BtsI GCAGTG_nn'
Cuts at: 445 992 445
Size: 547 3202
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 266 301 266
Size: 35 3714
DraI TTT'AAA
Cuts at: 748 748
Size: 3749
DraIII CAC_nnn'GTG
Cuts at: 612 612
Size: 3749
DrdI GACnn_nn'nnGTC
Cuts at: 1455 2799 1455
Size: 1344 2405
EaeI y'GGCC_r
Cuts at: 333 593 1334 1508 1899 1926 3271 333
Size: 260 741 174 391 27 1345 811
Fragments arranged by size:
1345 811 741 391 260 174 27
EagI C'GGCC_G
Cuts at: 1334 1334
Size: 3749
EarI CTCTTCn'nnn_
Cuts at: 221 1772 1982 221
Size: 1551 210 1988
Fragments arranged by size:
1988 1551 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1957 2699 2845 3559 1957
Size: 742 146 714 2147
Fragments arranged by size:
2147 742 714 146
EcoICRI GAG'CTC
Cuts at: 761 761
Size: 3749
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 328 354 466 475 1574 2006 2359 3021 328
Size: 26 112 9 1099 432 353 662 1056
Fragments arranged by size:
1099 1056 662 432 353 112 26 9
EcoNI CCTnn'n_nnAGG
Cuts at: 457 517 457
Size: 60 3689
EcoO109I rG'GnC_Cy
Cuts at: 125 450 712 888 125
Size: 325 262 176 2986
Fragments arranged by size:
2986 325 262 176
EcoRI G'AATT_C
Cuts at: 753 753
Size: 3749
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 225 252 438 1768 225
Size: 27 186 1330 2206
Fragments arranged by size:
2206 1330 186 27
FspI TGC'GCA
Cuts at: 1530 1530
Size: 3749
HaeII r_GCGC'y
Cuts at: 1183 1431 2661 3432 1183
Size: 248 1230 771 1500
Fragments arranged by size:
1500 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1591 1621 1623 1653 1591
Size: 30 2 30 3687
Fragments arranged by size:
3687 30 30 2
HincII GTy'rAC
Cuts at: 788 788
Size: 3749
HindIII A'AGCT_T
Cuts at: 310 310
Size: 3749
KasI G'GCGC_C
Cuts at: 1179 1427 1179
Size: 248 3501
KpnI G_GTAC'C
Cuts at: 769 769
Size: 3749
MluI A'CGCG_T
Cuts at: 3182 3182
Size: 3749
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 596 2508 2692 596
Size: 1912 184 1653
Fragments arranged by size:
1912 1653 184
MscI TGG'CCA
Cuts at: 335 1510 335
Size: 1175 2574
MslI CAynn'nnrTG
Cuts at: 280 1738 1865 280
Size: 1458 127 2164
Fragments arranged by size:
2164 1458 127
MspA1I CmG'CkG
Cuts at: 331 694 860 1174 1534 2318 2563 331
Size: 363 166 314 360 784 245 1517
Fragments arranged by size:
1517 784 363 360 314 245 166
NaeI GCC'GGC
Cuts at: 1930 1930
Size: 3749
NarI GG'CG_CC
Cuts at: 1180 1428 1180
Size: 248 3501
NcoI C'CATG_G
Cuts at: 107 107
Size: 3749
NgoMIV G'CCGG_C
Cuts at: 1928 1928
Size: 3749
NspI r_CATG'y
Cuts at: 802 1302 2905 802
Size: 500 1603 1646
Fragments arranged by size:
1646 1603 500
PasI CC'CwG_GG
Cuts at: 137 137
Size: 3749
PciI A'CATG_T
Cuts at: 2901 2901
Size: 3749
PfoI T'CCnGG_A
Cuts at: 539 539
Size: 3749
PmeI GTTT'AAAC
Cuts at: 748 748
Size: 3749
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2195 2227 2195
Size: 32 3717
PpuMI rG'GwC_Cy
Cuts at: 125 450 125
Size: 325 3424
PspOMI G'GGCC_C
Cuts at: 712 712
Size: 3749
PstI C_TGCA'G
Cuts at: 653 796 1481 653
Size: 143 685 2921
Fragments arranged by size:
2921 685 143
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3749
PvuII CAG'CTG
Cuts at: 331 694 1174 1534 331
Size: 363 480 360 2546
Fragments arranged by size:
2546 480 363 360
RsrII CG'GwC_CG
Cuts at: 1944 1944
Size: 3749
SacI G_AGCT'C
Cuts at: 763 763
Size: 3749
SalI G'TCGA_C
Cuts at: 786 786
Size: 3749
SapI GCTCTTCn'nnn_
Cuts at: 221 1772 1982 221
Size: 1551 210 1988
Fragments arranged by size:
1988 1551 210
SbfI CC_TGCA'GG
Cuts at: 796 796
Size: 3749
ScaI AGT'ACT
Cuts at: 3266 3266
Size: 3749
SexAI A'CCwGG_T
Cuts at: 608 608
Size: 3749
SfcI C'TryA_G
Cuts at: 33 649 792 1477 2445 2636 33
Size: 616 143 685 968 191 1146
Fragments arranged by size:
1146 968 685 616 191 143
SfoI GGC'GCC
Cuts at: 1181 1429 1181
Size: 248 3501
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3749
SmaI CCC'GGG
Cuts at: 771 771
Size: 3749
SmlI C'TyrA_G
Cuts at: 313 427 904 1819 2533 2795 313
Size: 114 477 915 714 262 1267
Fragments arranged by size:
1267 915 714 477 262 114
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3749
SphI G_CATG'C
Cuts at: 802 1302 802
Size: 500 3249
StyI C'CwwG_G
Cuts at: 107 883 1237 107
Size: 776 354 2619
Fragments arranged by size:
2619 776 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 283 485 1332 1465 1924 2133 283
Size: 202 847 133 459 209 1899
Fragments arranged by size:
1899 847 459 209 202 133
TatI w'GTAC_w
Cuts at: 3264 3264
Size: 3749
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3266 3298 3266
Size: 32 3717
Tth111I GACn'n_nGTC
Cuts at: 1546 1546
Size: 3749
XbaI T'CTAG_A
Cuts at: 780 780
Size: 3749
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1244 1244
Size: 3749
XmaI C'CCGG_G
Cuts at: 769 769
Size: 3749
XmnI GAAnn'nnTTC
Cuts at: 235 235
Size: 3749
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AleI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbvCI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PfoI PmeI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AlfI AloI
AscI AvrII BaeI BbsI BcgI BfrBI BmgBI BmtI
BsaXI BsiWI BsmI BsmBI BspEI BsrGI BssHII BstXI
BstZ17I Bsu36I ClaI EcoRV FseI FspAI HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PflMI PmlI
PshAI PsiI PspXI PsrI SacII SanDI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI ZraI