Restriction map for
pF1KB8607
(Circular) (Six-base) MAPSORT of: pF1KB8607.seq Check: 8431 from: 1 to: 5171
pF1KB8607 5171 bp
With 182 enzymes: SgfI *
July 15, 2010 18:27 ..
AccI GT'mk_AC
Cuts at: 359 2209 359
Size: 1850 3321
Acc65I G'GTAC_C
Cuts at: 2187 2187
Size: 5171
AclI AA'CG_TT
Cuts at: 5011 5011
Size: 5171
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 455 1586 2996 3428 4175 4443 455
Size: 1131 1410 432 747 268 1183
Fragments arranged by size:
1410 1183 1131 747 432 268
AflIII A'CryG_T
Cuts at: 758 1211 1766 1871 2523 3627 4604 758
Size: 453 555 105 652 1104 977 1325
Fragments arranged by size:
1325 1104 977 652 555 453 105
AgeI A'CCGG_T
Cuts at: 3559 3559
Size: 5171
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1909 1909
Size: 5171
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 967 999 967
Size: 32 5139
AlwNI CAG_nnn'CTG
Cuts at: 2476 4043 2476
Size: 1567 3604
ApaI G_GGCC'C
Cuts at: 1313 1313
Size: 5171
ApaLI G'TGCA_C
Cuts at: 3941 3941
Size: 5171
ApoI r'AATT_y
Cuts at: 520 2080 2175 4524 4780 520
Size: 1560 95 2349 256 911
Fragments arranged by size:
2349 1560 911 256 95
AseI AT'TA_AT
Cuts at: 20 20
Size: 5171
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5171
AvaI C'yCGr_G
Cuts at: 902 1190 2191 902
Size: 288 1001 3882
Fragments arranged by size:
3882 1001 288
BamHI G'GATC_C
Cuts at: 2196 2196
Size: 5171
BanI G'GyrC_C
Cuts at: 157 2187 2601 2849 2884 157
Size: 2030 414 248 35 2444
Fragments arranged by size:
2444 2030 414 248 35
BanII G_rGCy'C
Cuts at: 1313 2185 3215 1313
Size: 872 1030 3269
Fragments arranged by size:
3269 1030 872
BbeI G_GCGC'C
Cuts at: 2605 2853 2605
Size: 248 4923
BbsI GAAGACnn'nnnn_
Cuts at: 1928 1928
Size: 5171
BbvCI CC'TCA_GC
Cuts at: 332 332
Size: 5171
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 970 1004 90
Size: 34 846 34 4257
Fragments arranged by size:
4257 846 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 267 3069 3830 5171 267
Size: 2802 761 1341 267
Fragments arranged by size:
2802 1341 761 267
BclI T'GATC_A
Cuts at: 2691 2691
Size: 5171
BglI GCCn_nnn'nGGC
Cuts at: 835 835
Size: 5171
BglII A'GATC_T
Cuts at: 1146 1433 2686 4723 1146
Size: 287 1253 2037 1594
Fragments arranged by size:
2037 1594 1253 287
BlpI GC'TnA_GC
Cuts at: 2283 2283
Size: 5171
Bme1580I G_kGCm'C
Cuts at: 162 1313 1888 2796 2889 3945 162
Size: 1151 575 908 93 1056 1388
Fragments arranged by size:
1388 1151 1056 908 575 93
BmrI ACTGGGnnnn_n'
Cuts at: 757 1760 2383 2464 2799 4943 757
Size: 1003 623 81 335 2144 985
Fragments arranged by size:
2144 1003 985 623 335 81
BmtI G_CTAG'C
Cuts at: 1261 1261
Size: 5171
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3538 3538
Size: 5171
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1244 1646 1244
Size: 402 4769
Bpu10I CC'TnA_GC
Cuts at: 332 461 1242 332
Size: 129 781 4261
Fragments arranged by size:
4261 781 129
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2347 3226 3718 4016 2347
Size: 879 492 298 3502
Fragments arranged by size:
3502 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1220 4875 1220
Size: 3655 1516
BsaAI yAC'GTr
Cuts at: 765 2524 765
Size: 1759 3412
BsaBI GATnn'nnATC
Cuts at: 6 2685 4722 6
Size: 2679 2037 455
Fragments arranged by size:
2679 2037 455
BsaHI Gr'CG_yC
Cuts at: 122 2602 2850 3552 5048 122
Size: 2480 248 702 1496 245
Fragments arranged by size:
2480 1496 702 248 245
BsaWI w'CCGG_w
Cuts at: 1679 2360 2881 3559 3833 3980 4391 1679
Size: 681 521 678 274 147 411 2459
Fragments arranged by size:
2459 681 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 373 403 967 997 1034 1064 1941 1971 373
Size: 30 564 30 37 30 877 30 3573
Fragments arranged by size:
3573 877 564 37 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 701 1713 1847 1962 2054 701
Size: 1012 134 115 92 3818
Fragments arranged by size:
3818 1012 134 115 92
BseYI C'CCAG_C
Cuts at: 175 397 2596 3354 3931 4427 175
Size: 222 2199 758 577 496 919
Fragments arranged by size:
2199 919 758 577 496 222
BsiEI CG_ry'CG
Cuts at: 104 2713 2759 3967 104
Size: 2609 46 1208 1308
Fragments arranged by size:
2609 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 202 1986 2185 2963 3153 3945 202
Size: 1784 199 778 190 792 1428
Fragments arranged by size:
1784 1428 792 778 199 190
BsmI GAATG_Cn'
Cuts at: 960 960
Size: 5171
Bsp1286I G_dGCh'C
Cuts at: 162 202 1313 1888 1986 2185 2796 2889 2963
Size: 40 1111 575 98 199 611 93 74
Cuts at: 2963 3153 3215 3945 162
Size: 190 62 730 1388
Fragments arranged by size:
1388 1111 730 611 575 199 190 98
93 74 62 40
BspEI T'CCGG_A
Cuts at: 4391 4391
Size: 5171
BspHI T'CATG_A
Cuts at: 1520 4347 1520
Size: 2827 2344
BspMI ACCTGCnnnn'nnnn_
Cuts at: 176 1262 2221 2435 2737 3118 176
Size: 1086 959 214 302 381 2229
Fragments arranged by size:
2229 1086 959 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3463 3517 5001 5166 50
Size: 3413 54 1484 165 55
Fragments arranged by size:
3413 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3083 3083
Size: 5171
BsrFI r'CCGG_y
Cuts at: 154 598 3169 3350 3559 154
Size: 444 2571 181 209 1766
Fragments arranged by size:
2571 1766 444 209 181
BsrGI T'GTAC_A
Cuts at: 1654 1769 1654
Size: 115 5056
BssSI C'ACGA_G
Cuts at: 3442 3800 3442
Size: 358 4813
BstAPI GCAn_nnn'nTGC
Cuts at: 144 2548 144
Size: 2404 2767
BstBI TT'CG_AA
Cuts at: 3532 3532
Size: 5171
BstXI CCAn_nnnn'nTGG
Cuts at: 1876 1876
Size: 5171
BstYI r'GATC_y
Cuts at: 1 393 863 1146 1433 2196 2663 2686 3021
Size: 392 470 283 287 763 467 23 335
Cuts at: 3021 3267 4279 4365 4377 4723 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 763 470 467 449 392 346 335
287 283 246 86 23 12
BtgI C'CryG_G
Cuts at: 235 824 833 1001 1118 235
Size: 589 9 168 117 4288
Fragments arranged by size:
4288 589 168 117 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 555 3121 3302 3465 555
Size: 2566 181 163 2261
Fragments arranged by size:
2566 2261 181 163
BtsI GCAGTG_nn'
Cuts at: 1083 1848 2414 1083
Size: 765 566 3840
Fragments arranged by size:
3840 765 566
DraI TTT'AAA
Cuts at: 245 1373 2170 245
Size: 1128 797 3246
Fragments arranged by size:
3246 1128 797
DrdI GACnn_nn'nnGTC
Cuts at: 2877 3735 2877
Size: 858 4313
EaeI y'GGCC_r
Cuts at: 2756 2930 3321 3348 4693 2756
Size: 174 391 27 1345 3234
Fragments arranged by size:
3234 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2756 2756
Size: 5171
EarI CTCTTCn'nnn_
Cuts at: 188 1403 1925 1947 3194 3404 188
Size: 1215 522 22 1247 210 1955
Fragments arranged by size:
1955 1247 1215 522 210 22
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3379 3689 3835 4981 3379
Size: 310 146 1146 3569
Fragments arranged by size:
3569 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2183 2183
Size: 5171
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 455 1244 1586 1646 2996 3428 4175 4443 455
Size: 789 342 60 1350 432 747 268 1183
Fragments arranged by size:
1350 1183 789 747 432 342 268 60
EcoNI CCTnn'n_nnAGG
Cuts at: 922 973 1829 922
Size: 51 856 4264
Fragments arranged by size:
4264 856 51
EcoO109I rG'GnC_Cy
Cuts at: 179 1310 2092 2310 179
Size: 1131 782 218 3040
Fragments arranged by size:
3040 1131 782 218
EcoRI G'AATT_C
Cuts at: 2175 2175
Size: 5171
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3190 3190
Size: 5171
FspI TGC'GCA
Cuts at: 2952 2952
Size: 5171
HaeII r_GCGC'y
Cuts at: 2605 2853 3875 4854 2605
Size: 248 1022 979 2922
Fragments arranged by size:
2922 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 373 405 1890 1922 3013 3043 3045 3075 373
Size: 32 1485 32 1091 30 2 30 2469
Fragments arranged by size:
2469 1485 1091 32 32 30 30 2
HincII GTy'rAC
Cuts at: 821 2210 821
Size: 1389 3782
HindIII A'AGCT_T
Cuts at: 435 435
Size: 5171
KasI G'GCGC_C
Cuts at: 2601 2849 2601
Size: 248 4923
KpnI G_GTAC'C
Cuts at: 2191 2191
Size: 5171
MfeI C'AATT_G
Cuts at: 1582 1582
Size: 5171
MluI A'CGCG_T
Cuts at: 4604 4604
Size: 5171
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 663 1165 1427 3842 4026 663
Size: 502 262 2415 184 1808
Fragments arranged by size:
2415 1808 502 262 184
MscI TGG'CCA
Cuts at: 2932 2932
Size: 5171
MslI CAynn'nnrTG
Cuts at: 268 638 763 1874 3160 3287 268
Size: 370 125 1111 1286 127 2152
Fragments arranged by size:
2152 1286 1111 370 127 125
MspA1I CmG'CkG
Cuts at: 175 405 479 656 1130 1688 2000 2282 2596
Size: 230 74 177 474 558 312 282 314
Cuts at: 2596 2956 3969 4214 175
Size: 360 1013 245 1132
Fragments arranged by size:
1132 1013 558 474 360 314 312 282
245 230 177 74
NaeI GCC'GGC
Cuts at: 3352 3352
Size: 5171
NarI GG'CG_CC
Cuts at: 2602 2850 2602
Size: 248 4923
NcoI C'CATG_G
Cuts at: 833 1001 833
Size: 168 5003
NdeI CA'TA_TG
Cuts at: 1787 1787
Size: 5171
NgoMIV G'CCGG_C
Cuts at: 3350 3350
Size: 5171
NheI G'CTAG_C
Cuts at: 1257 1257
Size: 5171
NspI r_CATG'y
Cuts at: 762 1215 1770 1875 2224 2724 3631 762
Size: 453 555 105 349 500 907 2302
Fragments arranged by size:
2302 907 555 500 453 349 105
PciI A'CATG_T
Cuts at: 758 1211 1766 1871 3627 758
Size: 453 555 105 1756 2302
Fragments arranged by size:
2302 1756 555 453 105
PflMI CCAn_nnn'nTGG
Cuts at: 798 798
Size: 5171
PfoI T'CCnGG_A
Cuts at: 791 791
Size: 5171
PmeI GTTT'AAAC
Cuts at: 2170 2170
Size: 5171
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 967 999 4310 4342 967
Size: 32 3311 32 1796
Fragments arranged by size:
3311 1796 32 32
PpuMI rG'GwC_Cy
Cuts at: 179 2092 179
Size: 1913 3258
PspOMI G'GGCC_C
Cuts at: 1309 1309
Size: 5171
PstI C_TGCA'G
Cuts at: 149 2218 2903 149
Size: 2069 685 2417
Fragments arranged by size:
2417 2069 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5171
PvuII CAG'CTG
Cuts at: 405 479 1130 1688 2000 2596 2956 405
Size: 74 651 558 312 596 360 2620
Fragments arranged by size:
2620 651 596 558 360 312 74
RsrII CG'GwC_CG
Cuts at: 3366 3366
Size: 5171
SacI G_AGCT'C
Cuts at: 2185 2185
Size: 5171
SalI G'TCGA_C
Cuts at: 2208 2208
Size: 5171
SapI GCTCTTCn'nnn_
Cuts at: 3194 3404 3194
Size: 210 4961
SbfI CC_TGCA'GG
Cuts at: 2218 2218
Size: 5171
ScaI AGT'ACT
Cuts at: 4688 4688
Size: 5171
SfcI C'TryA_G
Cuts at: 33 145 1235 2214 2899 3892 4083 33
Size: 112 1090 979 685 993 191 1121
Fragments arranged by size:
1121 1090 993 979 685 191 112
SfoI GGC'GCC
Cuts at: 2603 2851 2603
Size: 248 4923
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5171
SgrAI Cr'CCGG_yG
Cuts at: 154 154
Size: 5171
SmaI CCC'GGG
Cuts at: 2193 2193
Size: 5171
SmlI C'TyrA_G
Cuts at: 2326 3241 3733 3995 2326
Size: 915 492 262 3502
Fragments arranged by size:
3502 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5171
SphI G_CATG'C
Cuts at: 2224 2724 2224
Size: 500 4671
StuI AGG'CCT
Cuts at: 1780 1824 1780
Size: 44 5127
StyI C'CwwG_G
Cuts at: 833 1001 1037 1456 1508 2305 2659 833
Size: 168 36 419 52 797 354 3345
Fragments arranged by size:
3345 797 419 354 168 52 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2754 2887 3346 3555 2754
Size: 133 459 209 4370
Fragments arranged by size:
4370 459 209 133
TatI w'GTAC_w
Cuts at: 543 1633 1654 1769 4686 543
Size: 1090 21 115 2917 1028
Fragments arranged by size:
2917 1090 1028 115 21
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1941 1973 4688 4720 1941
Size: 32 2715 32 2392
Fragments arranged by size:
2715 2392 32 32
Tth111I GACn'n_nGTC
Cuts at: 883 2968 883
Size: 2085 3086
XbaI T'CTAG_A
Cuts at: 2202 2202
Size: 5171
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 831 1463 1792 2666 831
Size: 632 329 874 3336
Fragments arranged by size:
3336 874 632 329
XmaI C'CCGG_G
Cuts at: 2191 2191
Size: 5171
XmnI GAAnn'nnTTC
Cuts at: 787 2033 787
Size: 1246 3925
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NspI PciI PflMI PfoI
PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AscI AvrII
BaeI BfrBI BmgBI BsgI BsiWI BsmBI BssHII BstEII
BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI
HpaI NotI NruI NsiI PacI PasI PmlI PshAI
PsiI PspXI PsrI SacII SanDI SexAI SfiI SnaBI
SrfI SspI SwaI XhoI ZraI