Restriction map for
pF1KB8615
(Circular) (Six-base) MAPSORT of: pF1KB8615.seq Check: 1068 from: 1 to: 4577
pF1KB8615 4577 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AccI GT'mk_AC
Cuts at: 1615 1615
Size: 4577
Acc65I G'GTAC_C
Cuts at: 1593 1593
Size: 4577
AclI AA'CG_TT
Cuts at: 4417 4417
Size: 4577
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 949 1112 2402 2834 3187 3849 949
Size: 163 1290 432 353 662 1677
Fragments arranged by size:
1677 1290 662 432 353 163
AflIII A'CryG_T
Cuts at: 1929 3729 4010 1929
Size: 1800 281 2496
Fragments arranged by size:
2496 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 450 761 450
Size: 311 4266
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1021 1454 1021
Size: 433 4144
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 964 996 964
Size: 32 4545
AlwNI CAG_nnn'CTG
Cuts at: 463 1882 3320 463
Size: 1419 1438 1720
Fragments arranged by size:
1720 1438 1419
ApaI G_GGCC'C
Cuts at: 389 389
Size: 4577
ApaLI G'TGCA_C
Cuts at: 3415 3415
Size: 4577
ApoI r'AATT_y
Cuts at: 1581 3930 4186 1581
Size: 2349 256 1972
Fragments arranged by size:
2349 1972 256
AseI AT'TA_AT
Cuts at: 20 709 20
Size: 689 3888
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4577
AvaI C'yCGr_G
Cuts at: 889 1393 1597 889
Size: 504 204 3869
Fragments arranged by size:
3869 504 204
BamHI G'GATC_C
Cuts at: 1602 1602
Size: 4577
BanI G'GyrC_C
Cuts at: 1354 1593 2007 2255 2290 1354
Size: 239 414 248 35 3641
Fragments arranged by size:
3641 414 248 239 35
BanII G_rGCy'C
Cuts at: 389 890 1591 2621 389
Size: 501 701 1030 2345
Fragments arranged by size:
2345 1030 701 501
BbeI G_GCGC'C
Cuts at: 2011 2259 2011
Size: 248 4329
BbsI GAAGACnn'nnnn_
Cuts at: 1320 1393 1320
Size: 73 4504
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1430 1464 1430
Size: 34 4543
BciVI GTATCCnnnnn_n'
Cuts at: 2475 3531 4577 2475
Size: 1056 1046 2475
Fragments arranged by size:
2475 1056 1046
BclI T'GATC_A
Cuts at: 2097 2097
Size: 4577
BglII A'GATC_T
Cuts at: 146 503 2092 4129 146
Size: 357 1589 2037 594
Fragments arranged by size:
2037 1589 594 357
BlpI GC'TnA_GC
Cuts at: 1689 1689
Size: 4577
Bme1580I G_kGCm'C
Cuts at: 389 2202 2295 3419 389
Size: 1813 93 1124 1547
Fragments arranged by size:
1813 1547 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1291 1789 1870 2205 4349 1291
Size: 498 81 335 2144 1519
Fragments arranged by size:
2144 1519 498 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2944 2944
Size: 4577
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 535 969 1753 2632 3346 3644 535
Size: 434 784 879 714 298 1468
Fragments arranged by size:
1468 879 784 714 434 298
BsaI GGTCTCn'nnnn_
Cuts at: 4281 4281
Size: 4577
BsaAI yAC'GTr
Cuts at: 1930 1930
Size: 4577
BsaBI GATnn'nnATC
Cuts at: 6 2091 4128 6
Size: 2085 2037 455
Fragments arranged by size:
2085 2037 455
BsaHI Gr'CG_yC
Cuts at: 2008 2256 2958 4454 2008
Size: 248 702 1496 2131
Fragments arranged by size:
2131 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1766 2287 2965 3376 3523 3797 1766
Size: 521 678 411 147 274 2546
Fragments arranged by size:
2546 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 898 898
Size: 4577
BseYI C'CCAG_C
Cuts at: 1132 2002 2760 3425 3833 1132
Size: 870 758 665 408 1876
Fragments arranged by size:
1876 870 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 573 573
Size: 4577
BsiEI CG_ry'CG
Cuts at: 104 2119 2165 3395 104
Size: 2015 46 1230 1286
Fragments arranged by size:
2015 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 206 890 1591 2369 2559 3419 206
Size: 684 701 778 190 860 1364
Fragments arranged by size:
1364 860 778 701 684 190
BsmI GAATG_Cn'
Cuts at: 1026 1026
Size: 4577
Bsp1286I G_dGCh'C
Cuts at: 206 389 890 1591 2202 2295 2369 2559 2621
Size: 183 501 701 611 93 74 190 62
Cuts at: 2621 3419 206
Size: 798 1364
Fragments arranged by size:
1364 798 701 611 501 190 183 93
74 62
BspHI T'CATG_A
Cuts at: 617 3009 617
Size: 2392 2185
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1627 1841 2143 2524 1627
Size: 214 302 381 3680
Fragments arranged by size:
3680 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2869 2923 4407 4572 50
Size: 2819 54 1484 165 55
Fragments arranged by size:
2819 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2489 2489
Size: 4577
BsrFI r'CCGG_y
Cuts at: 1164 2575 2756 1164
Size: 1411 181 2985
Fragments arranged by size:
2985 1411 181
BssSI C'ACGA_G
Cuts at: 491 1509 2848 3556 491
Size: 1018 1339 708 1512
Fragments arranged by size:
1512 1339 1018 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1502 1954 1502
Size: 452 4125
BstBI TT'CG_AA
Cuts at: 2938 2938
Size: 4577
BstXI CCAn_nnnn'nTGG
Cuts at: 813 813
Size: 4577
BstYI r'GATC_y
Cuts at: 1 146 503 1183 1602 2069 2092 2427 2673
Size: 145 357 680 419 467 23 335 246
Cuts at: 2673 2979 2991 3077 4129 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1052 680 467 449 419 357 335 306
246 145 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 377 377
Size: 4577
BtgI C'CryG_G
Cuts at: 107 743 107
Size: 636 3941
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2527 2708 2871 2527
Size: 181 163 4233
Fragments arranged by size:
4233 181 163
BtsI GCAGTG_nn'
Cuts at: 264 1266 1820 264
Size: 1002 554 3021
Fragments arranged by size:
3021 1002 554
DraI TTT'AAA
Cuts at: 1576 1576
Size: 4577
DraIII CAC_nnn'GTG
Cuts at: 538 538
Size: 4577
DrdI GACnn_nn'nnGTC
Cuts at: 2283 3627 2283
Size: 1344 3233
EaeI y'GGCC_r
Cuts at: 830 1166 1568 2162 2336 2727 2754 4099 830
Size: 336 402 594 174 391 27 1345 1308
Fragments arranged by size:
1345 1308 594 402 391 336 174 27
EagI C'GGCC_G
Cuts at: 2162 2162
Size: 4577
EarI CTCTTCn'nnn_
Cuts at: 2600 2810 2600
Size: 210 4367
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2785 3527 3673 4387 2785
Size: 742 146 714 2975
Fragments arranged by size:
2975 742 714 146
EcoICRI GAG'CTC
Cuts at: 888 1589 888
Size: 701 3876
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 949 1112 2402 2834 3187 3849 949
Size: 163 1290 432 353 662 1677
Fragments arranged by size:
1677 1290 662 432 353 163
EcoNI CCTnn'n_nnAGG
Cuts at: 380 380
Size: 4577
EcoO109I rG'GnC_Cy
Cuts at: 385 386 1398 1460 1716 385
Size: 1 1012 62 256 3246
Fragments arranged by size:
3246 1012 256 62 1
EcoRI G'AATT_C
Cuts at: 1581 1581
Size: 4577
EcoRV GAT'ATC
Cuts at: 610 610
Size: 4577
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2596 2596
Size: 4577
FspI TGC'GCA
Cuts at: 1025 2358 1025
Size: 1333 3244
FspAI rTGC'GCAy
Cuts at: 1025 1025
Size: 4577
HaeII r_GCGC'y
Cuts at: 2011 2259 3489 4260 2011
Size: 248 1230 771 2328
Fragments arranged by size:
2328 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 436 468 599 631 747 779 1194 1226 2419
Size: 32 131 32 116 32 415 32 1193
Cuts at: 2419 2449 2451 2481 436
Size: 30 2 30 2532
Fragments arranged by size:
2532 1193 415 131 116 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1616 1616
Size: 4577
KasI G'GCGC_C
Cuts at: 2007 2255 2007
Size: 248 4329
KpnI G_GTAC'C
Cuts at: 1597 1597
Size: 4577
MluI A'CGCG_T
Cuts at: 4010 4010
Size: 4577
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 231 3336 3520 231
Size: 3105 184 1288
Fragments arranged by size:
3105 1288 184
MscI TGG'CCA
Cuts at: 2338 2338
Size: 4577
MslI CAynn'nnrTG
Cuts at: 131 2566 2693 131
Size: 2435 127 2015
Fragments arranged by size:
2435 2015 127
MspA1I CmG'CkG
Cuts at: 828 927 1688 2002 2362 3146 3391 828
Size: 99 761 314 360 784 245 2014
Fragments arranged by size:
2014 784 761 360 314 245 99
NaeI GCC'GGC
Cuts at: 1166 2758 1166
Size: 1592 2985
NarI GG'CG_CC
Cuts at: 2008 2256 2008
Size: 248 4329
NcoI C'CATG_G
Cuts at: 107 743 107
Size: 636 3941
NgoMIV G'CCGG_C
Cuts at: 1164 2756 1164
Size: 1592 2985
NspI r_CATG'y
Cuts at: 1630 2130 3733 1630
Size: 500 1603 2474
Fragments arranged by size:
2474 1603 500
PciI A'CATG_T
Cuts at: 3729 3729
Size: 4577
PmeI GTTT'AAAC
Cuts at: 1576 1576
Size: 4577
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3023 3055 3023
Size: 32 4545
PspOMI G'GGCC_C
Cuts at: 385 385
Size: 4577
PstI C_TGCA'G
Cuts at: 395 1000 1443 1624 2309 395
Size: 605 443 181 685 2663
Fragments arranged by size:
2663 685 605 443 181
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4577
PvuII CAG'CTG
Cuts at: 828 927 2002 2362 828
Size: 99 1075 360 3043
Fragments arranged by size:
3043 1075 360 99
RsrII CG'GwC_CG
Cuts at: 2772 2772
Size: 4577
SacI G_AGCT'C
Cuts at: 890 1591 890
Size: 701 3876
SalI G'TCGA_C
Cuts at: 1614 1614
Size: 4577
SapI GCTCTTCn'nnn_
Cuts at: 2600 2810 2600
Size: 210 4367
SbfI CC_TGCA'GG
Cuts at: 1624 1624
Size: 4577
ScaI AGT'ACT
Cuts at: 4094 4094
Size: 4577
SfcI C'TryA_G
Cuts at: 33 391 996 1439 1620 2305 3273 3464 33
Size: 358 605 443 181 685 968 191 1146
Fragments arranged by size:
1146 968 685 605 443 358 191 181
SfoI GGC'GCC
Cuts at: 2009 2257 2009
Size: 248 4329
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4577
SmaI CCC'GGG
Cuts at: 1599 1599
Size: 4577
SmlI C'TyrA_G
Cuts at: 514 984 1732 2647 3361 3623 514
Size: 470 748 915 714 262 1468
Fragments arranged by size:
1468 915 748 714 470 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4577
SphI G_CATG'C
Cuts at: 1630 2130 1630
Size: 500 4077
SspI AAT'ATT
Cuts at: 558 558
Size: 4577
StuI AGG'CCT
Cuts at: 375 375
Size: 4577
StyI C'CwwG_G
Cuts at: 107 743 1376 1711 2065 107
Size: 636 633 335 354 2619
Fragments arranged by size:
2619 636 633 354 335
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2160 2293 2752 2961 2160
Size: 133 459 209 3776
Fragments arranged by size:
3776 459 209 133
TatI w'GTAC_w
Cuts at: 4092 4092
Size: 4577
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4094 4126 4094
Size: 32 4545
Tth111I GACn'n_nGTC
Cuts at: 2374 2374
Size: 4577
XbaI T'CTAG_A
Cuts at: 1608 1608
Size: 4577
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2072 2072
Size: 4577
XmaI C'CCGG_G
Cuts at: 1597 1597
Size: 4577
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AhdI AlfI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI FspAI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PmeI
PpiI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AleI AscI AvrII
BaeI BbvCI BfrBI BglI BmgBI BmtI BpmI Bpu10I
BsaXI BsiWI BsmBI BspEI BsrGI BssHII BstEII BstZ17I
ClaI CspCI FseI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmlI
PpuMI PshAI PsiI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SwaI XhoI XmnI ZraI