Restriction map for
pF1KB8617
(Circular) (Six-base) MAPSORT of: pF1KB8617.seq Check: 7984 from: 1 to: 4970
pF1KB8617 4970 bp
With 182 enzymes: SgfI *
September 18, 2009 17:00 ..
AccI GT'mk_AC
Cuts at: 608 2008 608
Size: 1400 3570
Acc65I G'GTAC_C
Cuts at: 1986 1986
Size: 4970
AclI AA'CG_TT
Cuts at: 4810 4810
Size: 4970
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 187 389 622 2795 3227 3974 4242 187
Size: 202 233 2173 432 747 268 915
Fragments arranged by size:
2173 915 747 432 268 233 202
AfeI AGC'GCT
Cuts at: 403 403
Size: 4970
AflIII A'CryG_T
Cuts at: 1071 2322 3426 4403 1071
Size: 1251 1104 977 1638
Fragments arranged by size:
1638 1251 1104 977
AgeI A'CCGG_T
Cuts at: 3358 3358
Size: 4970
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 668 700 668
Size: 32 4938
AlwNI CAG_nnn'CTG
Cuts at: 672 853 1302 2275 3842 672
Size: 181 449 973 1567 1800
Fragments arranged by size:
1800 1567 973 449 181
ApaLI G'TGCA_C
Cuts at: 3740 3740
Size: 4970
ApoI r'AATT_y
Cuts at: 677 1412 1836 1974 4323 4579 677
Size: 735 424 138 2349 256 1068
Fragments arranged by size:
2349 1068 735 424 256 138
AseI AT'TA_AT
Cuts at: 20 20
Size: 4970
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4970
AvaI C'yCGr_G
Cuts at: 337 1990 337
Size: 1653 3317
BamHI G'GATC_C
Cuts at: 1810 1995 1810
Size: 185 4785
BanI G'GyrC_C
Cuts at: 780 1986 2400 2648 2683 780
Size: 1206 414 248 35 3067
Fragments arranged by size:
3067 1206 414 248 35
BanII G_rGCy'C
Cuts at: 377 1129 1984 3014 377
Size: 752 855 1030 2333
Fragments arranged by size:
2333 1030 855 752
BbeI G_GCGC'C
Cuts at: 2404 2652 2404
Size: 248 4722
BbsI GAAGACnn'nnnn_
Cuts at: 203 650 1388 203
Size: 447 738 3785
Fragments arranged by size:
3785 738 447
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 328 362 328
Size: 34 4936
BciVI GTATCCnnnnn_n'
Cuts at: 2868 3629 4970 2868
Size: 761 1341 2868
Fragments arranged by size:
2868 1341 761
BclI T'GATC_A
Cuts at: 1640 2490 1640
Size: 850 4120
BglI GCCn_nnn'nGGC
Cuts at: 305 305
Size: 4970
BglII A'GATC_T
Cuts at: 121 1479 2485 4522 121
Size: 1358 1006 2037 569
Fragments arranged by size:
2037 1358 1006 569
BlpI GC'TnA_GC
Cuts at: 2082 2082
Size: 4970
Bme1580I G_kGCm'C
Cuts at: 2595 2688 3744 2595
Size: 93 1056 3821
Fragments arranged by size:
3821 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 2182 2263 2598 4742 2182
Size: 81 335 2144 2410
Fragments arranged by size:
2410 2144 335 81
BmtI G_CTAG'C
Cuts at: 1291 1291
Size: 4970
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 935 3337 935
Size: 2402 2568
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1349 1427 1349
Size: 78 4892
Bpu10I CC'TnA_GC
Cuts at: 858 858
Size: 4970
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2146 3025 3517 3815 2146
Size: 879 492 298 3301
Fragments arranged by size:
3301 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4674 4674
Size: 4970
BsaAI yAC'GTr
Cuts at: 2323 2323
Size: 4970
BsaBI GATnn'nnATC
Cuts at: 6 2484 4521 6
Size: 2478 2037 455
Fragments arranged by size:
2478 2037 455
BsaHI Gr'CG_yC
Cuts at: 2401 2649 3351 4847 2401
Size: 248 702 1496 2524
Fragments arranged by size:
2524 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2159 2680 3358 3632 3779 4190 2159
Size: 521 678 274 147 411 2939
Fragments arranged by size:
2939 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 295 404 672 1386 2395 3153 3730 4226 295
Size: 109 268 714 1009 758 577 496 1039
Fragments arranged by size:
1039 1009 758 714 577 496 268 109
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 866 866
Size: 4970
BsiEI CG_ry'CG
Cuts at: 104 2512 2558 3766 104
Size: 2408 46 1208 1308
Fragments arranged by size:
2408 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 620 1129 1612 1984 2762 2952 3744 620
Size: 509 483 372 778 190 792 1846
Fragments arranged by size:
1846 792 778 509 483 372 190
BsmI GAATG_Cn'
Cuts at: 147 600 147
Size: 453 4517
Bsp1286I G_dGCh'C
Cuts at: 377 620 1129 1612 1984 2595 2688 2762 2952
Size: 243 509 483 372 611 93 74 190
Cuts at: 2952 3014 3744 377
Size: 62 730 1603
Fragments arranged by size:
1603 730 611 509 483 372 243 190
93 74 62
BspEI T'CCGG_A
Cuts at: 4190 4190
Size: 4970
BspHI T'CATG_A
Cuts at: 1851 4146 1851
Size: 2295 2675
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2020 2234 2536 2917 2020
Size: 214 302 381 4073
Fragments arranged by size:
4073 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3262 3316 4800 4965 50
Size: 3212 54 1484 165 55
Fragments arranged by size:
3212 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2882 2882
Size: 4970
BsrFI r'CCGG_y
Cuts at: 2968 3149 3358 2968
Size: 181 209 4580
Fragments arranged by size:
4580 209 181
BssSI C'ACGA_G
Cuts at: 3241 3599 3241
Size: 358 4612
BstAPI GCAn_nnn'nTGC
Cuts at: 2347 2347
Size: 4970
BstBI TT'CG_AA
Cuts at: 3331 3331
Size: 4970
BstXI CCAn_nnnn'nTGG
Cuts at: 1705 1705
Size: 4970
BstYI r'GATC_y
Cuts at: 1 121 1479 1810 1995 2462 2485 2820 3066
Size: 120 1358 331 185 467 23 335 246
Cuts at: 3066 4078 4164 4176 4522 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1358 1012 467 449 346 335 331 246
185 120 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 740 740
Size: 4970
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2920 3101 3264 2920
Size: 181 163 4626
Fragments arranged by size:
4626 181 163
BtsI GCAGTG_nn'
Cuts at: 2213 2213
Size: 4970
DraI TTT'AAA
Cuts at: 1418 1969 1418
Size: 551 4419
DrdI GACnn_nn'nnGTC
Cuts at: 2676 3534 2676
Size: 858 4112
EaeI y'GGCC_r
Cuts at: 306 839 2555 2729 3120 3147 4492 306
Size: 533 1716 174 391 27 1345 784
Fragments arranged by size:
1716 1345 784 533 391 174 27
EagI C'GGCC_G
Cuts at: 2555 2555
Size: 4970
EarI CTCTTCn'nnn_
Cuts at: 365 530 822 1704 1780 1855 2993 3203 365
Size: 165 292 882 76 75 1138 210 2132
Fragments arranged by size:
2132 1138 882 292 210 165 76 75
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3178 3488 3634 4780 3178
Size: 310 146 1146 3368
Fragments arranged by size:
3368 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1127 1982 1127
Size: 855 4115
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 187 389 622 1349 1427 2795 3227 3974 4242
Size: 202 233 727 78 1368 432 747 268
Cuts at: 4242 187
Size: 915
Fragments arranged by size:
1368 915 747 727 432 268 233 202
78
EcoO109I rG'GnC_Cy
Cuts at: 2109 2109
Size: 4970
EcoRI G'AATT_C
Cuts at: 1974 1974
Size: 4970
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1449 2989 1449
Size: 1540 3430
FspI TGC'GCA
Cuts at: 2751 2751
Size: 4970
HaeII r_GCGC'y
Cuts at: 405 2404 2652 3674 4653 405
Size: 1999 248 1022 979 722
Fragments arranged by size:
1999 1022 979 722 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 443 475 568 600 642 674 841 873 2812
Size: 32 93 32 42 32 167 32 1939
Cuts at: 2812 2842 2844 2874 443
Size: 30 2 30 2539
Fragments arranged by size:
2539 1939 167 93 42 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 2009 2009
Size: 4970
KasI G'GCGC_C
Cuts at: 2400 2648 2400
Size: 248 4722
KpnI G_GTAC'C
Cuts at: 1990 1990
Size: 4970
MluI A'CGCG_T
Cuts at: 4403 4403
Size: 4970
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 188 1294 3641 3825 188
Size: 1106 2347 184 1333
Fragments arranged by size:
2347 1333 1106 184
MscI TGG'CCA
Cuts at: 308 841 2731 308
Size: 533 1890 2547
Fragments arranged by size:
2547 1890 533
MslI CAynn'nnrTG
Cuts at: 796 2959 3086 796
Size: 2163 127 2680
Fragments arranged by size:
2680 2163 127
MspA1I CmG'CkG
Cuts at: 672 871 2081 2395 2755 3768 4013 672
Size: 199 1210 314 360 1013 245 1629
Fragments arranged by size:
1629 1210 1013 360 314 245 199
NaeI GCC'GGC
Cuts at: 3151 3151
Size: 4970
NarI GG'CG_CC
Cuts at: 2401 2649 2401
Size: 248 4722
NgoMIV G'CCGG_C
Cuts at: 3149 3149
Size: 4970
NheI G'CTAG_C
Cuts at: 1287 1287
Size: 4970
NspI r_CATG'y
Cuts at: 1075 2023 2523 3430 1075
Size: 948 500 907 2615
Fragments arranged by size:
2615 948 907 500
PasI CC'CwG_GG
Cuts at: 1096 1096
Size: 4970
PciI A'CATG_T
Cuts at: 1071 3426 1071
Size: 2355 2615
PmeI GTTT'AAAC
Cuts at: 1969 1969
Size: 4970
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4109 4141 4109
Size: 32 4938
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1728 1760 1728
Size: 32 4938
PstI C_TGCA'G
Cuts at: 2017 2702 2017
Size: 685 4285
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4970
PvuII CAG'CTG
Cuts at: 672 871 2395 2755 672
Size: 199 1524 360 2887
Fragments arranged by size:
2887 1524 360 199
RsrII CG'GwC_CG
Cuts at: 3165 3165
Size: 4970
SacI G_AGCT'C
Cuts at: 1129 1984 1129
Size: 855 4115
SalI G'TCGA_C
Cuts at: 2007 2007
Size: 4970
SapI GCTCTTCn'nnn_
Cuts at: 365 2993 3203 365
Size: 2628 210 2132
Fragments arranged by size:
2628 2132 210
SbfI CC_TGCA'GG
Cuts at: 2017 2017
Size: 4970
ScaI AGT'ACT
Cuts at: 4487 4487
Size: 4970
SfcI C'TryA_G
Cuts at: 33 239 245 1130 2013 2698 3691 3882 33
Size: 206 6 885 883 685 993 191 1121
Fragments arranged by size:
1121 993 885 883 685 206 191 6
SfoI GGC'GCC
Cuts at: 2402 2650 2402
Size: 248 4722
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4970
SmaI CCC'GGG
Cuts at: 1992 1992
Size: 4970
SmlI C'TyrA_G
Cuts at: 2125 3040 3532 3794 2125
Size: 915 492 262 3301
Fragments arranged by size:
3301 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4970
SphI G_CATG'C
Cuts at: 2023 2523 2023
Size: 500 4470
StuI AGG'CCT
Cuts at: 1631 1631
Size: 4970
StyI C'CwwG_G
Cuts at: 2104 2458 2104
Size: 354 4616
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2553 2686 3145 3354 2553
Size: 133 459 209 4169
Fragments arranged by size:
4169 459 209 133
TatI w'GTAC_w
Cuts at: 4485 4485
Size: 4970
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4487 4519 4487
Size: 32 4938
Tth111I GACn'n_nGTC
Cuts at: 2767 2767
Size: 4970
XbaI T'CTAG_A
Cuts at: 2001 2001
Size: 4970
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1185 2465 1185
Size: 1280 3690
XmaI C'CCGG_G
Cuts at: 1990 1990
Size: 4970
XmnI GAAnn'nnTTC
Cuts at: 200 1226 1496 200
Size: 1026 270 3674
Fragments arranged by size:
3674 1026 270
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NheI NspI PasI PciI PmeI PpiI
PsrI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI ApaI AscI
AvrII BaeI BbvCI BfrBI BmgBI BsaXI BseRI BsiWI
BsmBI BsrGI BssHII BstEII BstZ17I BtgI ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NcoI NdeI NotI NruI NsiI PacI PflMI PfoI
PmlI PpuMI PshAI PsiI PspOMI PspXI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
ZraI