Restriction map for
pF1KB8620
(Circular) (Six-base) MAPSORT of: pF1KB8620.seq Check: 8452 from: 1 to: 3665
pF1KB8620 3665 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AccI GT'mk_AC
Cuts at: 703 703
Size: 3665
Acc65I G'GTAC_C
Cuts at: 681 681
Size: 3665
AclI AA'CG_TT
Cuts at: 3505 3505
Size: 3665
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1490 1922 2275 2937 1490
Size: 432 353 662 2218
Fragments arranged by size:
2218 662 432 353
AflIII A'CryG_T
Cuts at: 1017 2817 3098 1017
Size: 1800 281 1584
Fragments arranged by size:
1800 1584 281
AleI CACnn'nnGTG
Cuts at: 255 255
Size: 3665
AlwNI CAG_nnn'CTG
Cuts at: 622 970 2408 622
Size: 348 1438 1879
Fragments arranged by size:
1879 1438 348
ApaLI G'TGCA_C
Cuts at: 2503 2503
Size: 3665
ApoI r'AATT_y
Cuts at: 185 669 3018 3274 185
Size: 484 2349 256 576
Fragments arranged by size:
2349 576 484 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3665
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3665
AvaI C'yCGr_G
Cuts at: 685 685
Size: 3665
BamHI G'GATC_C
Cuts at: 690 690
Size: 3665
BanI G'GyrC_C
Cuts at: 681 1095 1343 1378 681
Size: 414 248 35 2968
Fragments arranged by size:
2968 414 248 35
BanII G_rGCy'C
Cuts at: 679 1709 679
Size: 1030 2635
BbeI G_GCGC'C
Cuts at: 1099 1347 1099
Size: 248 3417
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3631
BciVI GTATCCnnnnn_n'
Cuts at: 270 1563 2619 3665 270
Size: 1293 1056 1046 270
Fragments arranged by size:
1293 1056 1046 270
BclI T'GATC_A
Cuts at: 1185 1185
Size: 3665
BglII A'GATC_T
Cuts at: 575 1180 3217 575
Size: 605 2037 1023
Fragments arranged by size:
2037 1023 605
BlpI GC'TnA_GC
Cuts at: 777 777
Size: 3665
Bme1580I G_kGCm'C
Cuts at: 1290 1383 2507 1290
Size: 93 1124 2448
Fragments arranged by size:
2448 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 877 958 1293 3437 877
Size: 81 335 2144 1105
Fragments arranged by size:
2144 1105 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2032 2032
Size: 3665
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 599 599
Size: 3665
Bpu10I CC'TnA_GC
Cuts at: 138 138
Size: 3665
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 841 1720 2434 2732 841
Size: 879 714 298 1774
Fragments arranged by size:
1774 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 3369 3369
Size: 3665
BsaAI yAC'GTr
Cuts at: 1018 1018
Size: 3665
BsaBI GATnn'nnATC
Cuts at: 6 1179 3216 6
Size: 1173 2037 455
Fragments arranged by size:
2037 1173 455
BsaHI Gr'CG_yC
Cuts at: 1096 1344 2046 3542 1096
Size: 248 702 1496 1219
Fragments arranged by size:
1496 1219 702 248
BsaWI w'CCGG_w
Cuts at: 854 1375 2053 2464 2611 2885 854
Size: 521 678 411 147 274 1634
Fragments arranged by size:
1634 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1090 1848 2513 2921 1090
Size: 758 665 408 1834
Fragments arranged by size:
1834 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 1207 1253 2483 104
Size: 1103 46 1230 1286
Fragments arranged by size:
1286 1230 1103 46
BsiHKAI G_wGCw'C
Cuts at: 679 1457 1647 2507 679
Size: 778 190 860 1837
Fragments arranged by size:
1837 860 778 190
BsmI GAATG_Cn'
Cuts at: 532 532
Size: 3665
Bsp1286I G_dGCh'C
Cuts at: 679 1290 1383 1457 1647 1709 2507 679
Size: 611 93 74 190 62 798 1837
Fragments arranged by size:
1837 798 611 190 93 74 62
BspHI T'CATG_A
Cuts at: 2097 2097
Size: 3665
BspMI ACCTGCnnnn'nnnn_
Cuts at: 715 929 1231 1612 715
Size: 214 302 381 2768
Fragments arranged by size:
2768 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1957 2011 3495 3660 50
Size: 1907 54 1484 165 55
Fragments arranged by size:
1907 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 311 1577 311
Size: 1266 2399
BsrFI r'CCGG_y
Cuts at: 1663 1844 1663
Size: 181 3484
BsrGI T'GTAC_A
Cuts at: 168 168
Size: 3665
BssSI C'ACGA_G
Cuts at: 1936 2644 1936
Size: 708 2957
BstAPI GCAn_nnn'nTGC
Cuts at: 1042 1042
Size: 3665
BstBI TT'CG_AA
Cuts at: 2026 2026
Size: 3665
BstYI r'GATC_y
Cuts at: 1 575 690 1157 1180 1515 1761 2067 2079
Size: 574 115 467 23 335 246 306 12
Cuts at: 2079 2165 3217 1
Size: 86 1052 449
Fragments arranged by size:
1052 574 467 449 335 306 246 115
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1615 1796 1959 1615
Size: 181 163 3321
Fragments arranged by size:
3321 181 163
BtsI GCAGTG_nn'
Cuts at: 908 908
Size: 3665
DraI TTT'AAA
Cuts at: 664 664
Size: 3665
DrdI GACnn_nn'nnGTC
Cuts at: 1371 2715 1371
Size: 1344 2321
EaeI y'GGCC_r
Cuts at: 1250 1424 1815 1842 3187 1250
Size: 174 391 27 1345 1728
Fragments arranged by size:
1728 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1250 1250
Size: 3665
EarI CTCTTCn'nnn_
Cuts at: 1688 1898 1688
Size: 210 3455
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1873 2615 2761 3475 1873
Size: 742 146 714 2063
Fragments arranged by size:
2063 742 714 146
EcoICRI GAG'CTC
Cuts at: 677 677
Size: 3665
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 599 1490 1922 2275 2937 599
Size: 891 432 353 662 1327
Fragments arranged by size:
1327 891 662 432 353
EcoO109I rG'GnC_Cy
Cuts at: 804 804
Size: 3665
EcoRI G'AATT_C
Cuts at: 669 669
Size: 3665
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 480 1684 480
Size: 1204 2461
FspI TGC'GCA
Cuts at: 1446 1446
Size: 3665
HaeII r_GCGC'y
Cuts at: 1099 1347 2577 3348 1099
Size: 248 1230 771 1416
Fragments arranged by size:
1416 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1507 1537 1539 1569 1507
Size: 30 2 30 3603
Fragments arranged by size:
3603 30 30 2
HincII GTy'rAC
Cuts at: 704 704
Size: 3665
KasI G'GCGC_C
Cuts at: 1095 1343 1095
Size: 248 3417
KpnI G_GTAC'C
Cuts at: 685 685
Size: 3665
MluI A'CGCG_T
Cuts at: 3098 3098
Size: 3665
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 129 2424 2608 129
Size: 2295 184 1186
Fragments arranged by size:
2295 1186 184
MscI TGG'CCA
Cuts at: 1426 1426
Size: 3665
MslI CAynn'nnrTG
Cuts at: 255 1654 1781 255
Size: 1399 127 2139
Fragments arranged by size:
2139 1399 127
MspA1I CmG'CkG
Cuts at: 654 776 1090 1450 2234 2479 654
Size: 122 314 360 784 245 1840
Fragments arranged by size:
1840 784 360 314 245 122
NaeI GCC'GGC
Cuts at: 1846 1846
Size: 3665
NarI GG'CG_CC
Cuts at: 1096 1344 1096
Size: 248 3417
NgoMIV G'CCGG_C
Cuts at: 1844 1844
Size: 3665
NspI r_CATG'y
Cuts at: 718 1218 2821 718
Size: 500 1603 1562
Fragments arranged by size:
1603 1562 500
PciI A'CATG_T
Cuts at: 2817 2817
Size: 3665
PflMI CCAn_nnn'nTGG
Cuts at: 622 622
Size: 3665
PmeI GTTT'AAAC
Cuts at: 664 664
Size: 3665
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2111 2143 2111
Size: 32 3633
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 161 193 161
Size: 32 3633
PstI C_TGCA'G
Cuts at: 285 712 1397 285
Size: 427 685 2553
Fragments arranged by size:
2553 685 427
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3665
PvuII CAG'CTG
Cuts at: 654 1090 1450 654
Size: 436 360 2869
Fragments arranged by size:
2869 436 360
RsrII CG'GwC_CG
Cuts at: 1860 1860
Size: 3665
SacI G_AGCT'C
Cuts at: 679 679
Size: 3665
SalI G'TCGA_C
Cuts at: 702 702
Size: 3665
SapI GCTCTTCn'nnn_
Cuts at: 1688 1898 1688
Size: 210 3455
SbfI CC_TGCA'GG
Cuts at: 712 712
Size: 3665
ScaI AGT'ACT
Cuts at: 3182 3182
Size: 3665
SfcI C'TryA_G
Cuts at: 33 281 708 1393 2361 2552 33
Size: 248 427 685 968 191 1146
Fragments arranged by size:
1146 968 685 427 248 191
SfoI GGC'GCC
Cuts at: 1097 1345 1097
Size: 248 3417
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3665
SmaI CCC'GGG
Cuts at: 687 687
Size: 3665
SmlI C'TyrA_G
Cuts at: 820 1735 2449 2711 820
Size: 915 714 262 1774
Fragments arranged by size:
1774 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3665
SphI G_CATG'C
Cuts at: 718 1218 718
Size: 500 3165
StyI C'CwwG_G
Cuts at: 799 1153 799
Size: 354 3311
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1248 1381 1840 2049 1248
Size: 133 459 209 2864
Fragments arranged by size:
2864 459 209 133
TatI w'GTAC_w
Cuts at: 168 3180 168
Size: 3012 653
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 513 545 3182 3214 513
Size: 32 2637 32 964
Fragments arranged by size:
2637 964 32 32
Tth111I GACn'n_nGTC
Cuts at: 1462 1462
Size: 3665
XbaI T'CTAG_A
Cuts at: 696 696
Size: 3665
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1160 1160
Size: 3665
XmaI C'CCGG_G
Cuts at: 685 685
Size: 3665
XmnI GAAnn'nnTTC
Cuts at: 404 404
Size: 3665
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AleI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BcgI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PciI PflMI PmeI PpiI PsrI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BfrBI BglI
BmgBI BmtI BsaXI BseRI BsgI BsiWI BsmBI BspEI
BssHII BstEII BstXI BstZ17I Bsu36I BtgI ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PasI
PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI ZraI