Restriction map for
pF1KB8627
(Circular) (Six-base) MAPSORT of: pF1KB8627.seq Check: 6371 from: 1 to: 5435
pF1KB8627 5435 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AccI GT'mk_AC
Cuts at: 1746 2473 1746
Size: 727 4708
Acc65I G'GTAC_C
Cuts at: 2451 2451
Size: 5435
AclI AA'CG_TT
Cuts at: 703 5275 703
Size: 4572 863
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 337 604 881 1336 1515 1690 3260 3692 4045
Size: 267 277 455 179 175 1570 432 353
Cuts at: 4045 4707 337
Size: 662 1065
Fragments arranged by size:
1570 1065 662 455 432 353 277 267
179 175
AfeI AGC'GCT
Cuts at: 2409 2409
Size: 5435
AflII C'TTAA_G
Cuts at: 1246 1246
Size: 5435
AflIII A'CryG_T
Cuts at: 2787 4587 4868 2787
Size: 1800 281 3354
Fragments arranged by size:
3354 1800 281
AleI CACnn'nnGTG
Cuts at: 1870 1870
Size: 5435
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1843 2159 1843
Size: 316 5119
AlwNI CAG_nnn'CTG
Cuts at: 1432 2235 2740 4178 1432
Size: 803 505 1438 2689
Fragments arranged by size:
2689 1438 803 505
ApaLI G'TGCA_C
Cuts at: 4273 4273
Size: 5435
ApoI r'AATT_y
Cuts at: 487 2044 2439 4788 5044 487
Size: 1557 395 2349 256 878
Fragments arranged by size:
2349 1557 878 395 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5435
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5435
AvaI C'yCGr_G
Cuts at: 220 235 1418 2310 2455 220
Size: 15 1183 892 145 3200
Fragments arranged by size:
3200 1183 892 145 15
BamHI G'GATC_C
Cuts at: 2460 2460
Size: 5435
BanI G'GyrC_C
Cuts at: 362 2135 2451 2865 3113 3148 362
Size: 1773 316 414 248 35 2649
Fragments arranged by size:
2649 1773 414 316 248 35
BanII G_rGCy'C
Cuts at: 1668 1850 1964 2449 3479 1668
Size: 182 114 485 1030 3624
Fragments arranged by size:
3624 1030 485 182 114
BbeI G_GCGC'C
Cuts at: 2869 3117 2869
Size: 248 5187
BbsI GAAGACnn'nnnn_
Cuts at: 457 457
Size: 5435
BbvCI CC'TCA_GC
Cuts at: 194 194
Size: 5435
BciVI GTATCCnnnnn_n'
Cuts at: 3333 4389 5435 3333
Size: 1056 1046 3333
Fragments arranged by size:
3333 1056 1046
BclI T'GATC_A
Cuts at: 2955 2955
Size: 5435
BglI GCCn_nnn'nGGC
Cuts at: 1777 2071 1777
Size: 294 5141
BglII A'GATC_T
Cuts at: 561 2004 2950 4987 561
Size: 1443 946 2037 1009
Fragments arranged by size:
2037 1443 1009 946
BlpI GC'TnA_GC
Cuts at: 2547 2547
Size: 5435
Bme1580I G_kGCm'C
Cuts at: 2067 2311 2317 3060 3153 4277 2067
Size: 244 6 743 93 1124 3225
Fragments arranged by size:
3225 1124 743 244 93 6
BmrI ACTGGGnnnn_n'
Cuts at: 955 1690 2647 2728 3063 5207 955
Size: 735 957 81 335 2144 1183
Fragments arranged by size:
2144 1183 957 735 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3802 3802
Size: 5435
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 578 1943 2292 578
Size: 1365 349 3721
Fragments arranged by size:
3721 1365 349
Bpu10I CC'TnA_GC
Cuts at: 194 194
Size: 5435
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1120 1516 2611 3490 4204 4502 1120
Size: 396 1095 879 714 298 2053
Fragments arranged by size:
2053 1095 879 714 396 298
BsaI GGTCTCn'nnnn_
Cuts at: 5139 5139
Size: 5435
BsaAI yAC'GTr
Cuts at: 2788 2788
Size: 5435
BsaBI GATnn'nnATC
Cuts at: 6 1049 1600 2949 4986 6
Size: 1043 551 1349 2037 455
Fragments arranged by size:
2037 1349 1043 551 455
BsaHI Gr'CG_yC
Cuts at: 2866 3114 3816 5312 2866
Size: 248 702 1496 2989
Fragments arranged by size:
2989 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2624 3145 3823 4234 4381 4655 2624
Size: 521 678 411 147 274 3404
Fragments arranged by size:
3404 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 238 1594 238
Size: 1356 4079
BseYI C'CCAG_C
Cuts at: 355 1633 1772 2066 2860 3618 4283 4691 355
Size: 1278 139 294 794 758 665 408 1099
Fragments arranged by size:
1278 1099 794 758 665 408 294 139
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1849 1849
Size: 5435
BsiEI CG_ry'CG
Cuts at: 104 2977 3023 4253 104
Size: 2873 46 1230 1286
Fragments arranged by size:
2873 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1062 1850 2353 2449 3227 3417 4277 1062
Size: 788 503 96 778 190 860 2220
Fragments arranged by size:
2220 860 788 778 503 190 96
BsmI GAATG_Cn'
Cuts at: 475 1384 475
Size: 909 4526
Bsp1286I G_dGCh'C
Cuts at: 1062 1668 1850 1964 2067 2311 2317 2353 2449
Size: 606 182 114 103 244 6 36 96
Cuts at: 2449 3060 3153 3227 3417 3479 4277 1062
Size: 611 93 74 190 62 798 2220
Fragments arranged by size:
2220 798 611 606 244 190 182 114
103 96 93 74 62 36 6
BspHI T'CATG_A
Cuts at: 1194 3867 1194
Size: 2673 2762
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2485 2699 3001 3382 2485
Size: 214 302 381 4538
Fragments arranged by size:
4538 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3727 3781 5265 5430 50
Size: 3677 54 1484 165 55
Fragments arranged by size:
3677 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3347 3347
Size: 5435
BsrFI r'CCGG_y
Cuts at: 208 3433 3614 208
Size: 3225 181 2029
Fragments arranged by size:
3225 2029 181
BsrGI T'GTAC_A
Cuts at: 984 984
Size: 5435
BssSI C'ACGA_G
Cuts at: 1870 3706 4414 1870
Size: 1836 708 2891
Fragments arranged by size:
2891 1836 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1652 2321 2812 1652
Size: 669 491 4275
Fragments arranged by size:
4275 669 491
BstBI TT'CG_AA
Cuts at: 468 3796 468
Size: 3328 2107
BstXI CCAn_nnnn'nTGG
Cuts at: 576 576
Size: 5435
BstYI r'GATC_y
Cuts at: 1 216 561 1050 2004 2460 2927 2950 3285
Size: 215 345 489 954 456 467 23 335
Cuts at: 3285 3531 3837 3849 3935 4987 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1052 954 489 467 456 449 345 335
306 246 215 86 23 12
BstZ17I GTA'TAC
Cuts at: 1747 1747
Size: 5435
BtgI C'CryG_G
Cuts at: 107 107
Size: 5435
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 214 3385 3566 3729 214
Size: 3171 181 163 1920
Fragments arranged by size:
3171 1920 181 163
BtsI GCAGTG_nn'
Cuts at: 1003 2067 2678 1003
Size: 1064 611 3760
Fragments arranged by size:
3760 1064 611
DraI TTT'AAA
Cuts at: 1683 2434 1683
Size: 751 4684
DrdI GACnn_nn'nnGTC
Cuts at: 3141 4485 3141
Size: 1344 4091
EaeI y'GGCC_r
Cuts at: 1778 3020 3194 3585 3612 4957 1778
Size: 1242 174 391 27 1345 2256
Fragments arranged by size:
2256 1345 1242 391 174 27
EagI C'GGCC_G
Cuts at: 3020 3020
Size: 5435
EarI CTCTTCn'nnn_
Cuts at: 1666 3458 3668 1666
Size: 1792 210 3433
Fragments arranged by size:
3433 1792 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3643 4385 4531 5245 3643
Size: 742 146 714 3833
Fragments arranged by size:
3833 742 714 146
EcoICRI GAG'CTC
Cuts at: 1848 2447 1848
Size: 599 4836
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 337 578 604 881 1336 1515 1690 1943 2292
Size: 241 26 277 455 179 175 253 349
Cuts at: 2292 3260 3692 4045 4707 337
Size: 968 432 353 662 1065
Fragments arranged by size:
1065 968 662 455 432 353 349 277
253 241 179 175 26
EcoNI CCTnn'n_nnAGG
Cuts at: 1576 1576
Size: 5435
EcoO109I rG'GnC_Cy
Cuts at: 168 583 2073 2574 168
Size: 415 1490 501 3029
Fragments arranged by size:
3029 1490 501 415
EcoRI G'AATT_C
Cuts at: 2439 2439
Size: 5435
EcoRV GAT'ATC
Cuts at: 1619 1619
Size: 5435
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1024 1510 3454 1024
Size: 486 1944 3005
Fragments arranged by size:
3005 1944 486
FspI TGC'GCA
Cuts at: 3216 3216
Size: 5435
HaeII r_GCGC'y
Cuts at: 2271 2411 2869 3117 4347 5118 2271
Size: 140 458 248 1230 771 2588
Fragments arranged by size:
2588 1230 771 458 248 140
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1118 1150 1499 1531 3277 3307 3309 3339 1118
Size: 32 349 32 1746 30 2 30 3214
Fragments arranged by size:
3214 1746 349 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2474 2474
Size: 5435
HindIII A'AGCT_T
Cuts at: 1402 1686 1402
Size: 284 5151
KasI G'GCGC_C
Cuts at: 2865 3113 2865
Size: 248 5187
KpnI G_GTAC'C
Cuts at: 2455 2455
Size: 5435
MluI A'CGCG_T
Cuts at: 4868 4868
Size: 5435
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4194 4378 4194
Size: 184 5251
MscI TGG'CCA
Cuts at: 1780 3196 1780
Size: 1416 4019
MslI CAynn'nnrTG
Cuts at: 1079 1825 1870 2141 3424 3551 1079
Size: 746 45 271 1283 127 2963
Fragments arranged by size:
2963 1283 746 271 127 45
MspA1I CmG'CkG
Cuts at: 193 303 359 1776 2546 2860 3220 4004 4249
Size: 110 56 1417 770 314 360 784 245
Cuts at: 4249 193
Size: 1379
Fragments arranged by size:
1417 1379 784 770 360 314 245 110
56
NaeI GCC'GGC
Cuts at: 3616 3616
Size: 5435
NarI GG'CG_CC
Cuts at: 2866 3114 2866
Size: 248 5187
NcoI C'CATG_G
Cuts at: 107 107
Size: 5435
NdeI CA'TA_TG
Cuts at: 1080 1080
Size: 5435
NgoMIV G'CCGG_C
Cuts at: 3614 3614
Size: 5435
NspI r_CATG'y
Cuts at: 2122 2488 2988 4591 2122
Size: 366 500 1603 2966
Fragments arranged by size:
2966 1603 500 366
PasI CC'CwG_GG
Cuts at: 2185 2185
Size: 5435
PciI A'CATG_T
Cuts at: 4587 4587
Size: 5435
PmeI GTTT'AAAC
Cuts at: 2434 2434
Size: 5435
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3881 3913 3881
Size: 32 5403
PshAI GACnn'nnGTC
Cuts at: 782 782
Size: 5435
PstI C_TGCA'G
Cuts at: 416 2482 3167 416
Size: 2066 685 2684
Fragments arranged by size:
2684 2066 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5435
PvuII CAG'CTG
Cuts at: 193 303 359 1776 2860 3220 193
Size: 110 56 1417 1084 360 2408
Fragments arranged by size:
2408 1417 1084 360 110 56
RsrII CG'GwC_CG
Cuts at: 3630 3630
Size: 5435
SacI G_AGCT'C
Cuts at: 1850 2449 1850
Size: 599 4836
SalI G'TCGA_C
Cuts at: 2472 2472
Size: 5435
SapI GCTCTTCn'nnn_
Cuts at: 3458 3668 3458
Size: 210 5225
SbfI CC_TGCA'GG
Cuts at: 2482 2482
Size: 5435
ScaI AGT'ACT
Cuts at: 1349 4952 1349
Size: 3603 1832
SfcI C'TryA_G
Cuts at: 33 412 770 2478 3163 4131 4322 33
Size: 379 358 1708 685 968 191 1146
Fragments arranged by size:
1708 1146 968 685 379 358 191
SfoI GGC'GCC
Cuts at: 2867 3115 2867
Size: 248 5187
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5435
SmaI CCC'GGG
Cuts at: 1420 2312 2457 1420
Size: 892 145 4398
Fragments arranged by size:
4398 892 145
SmlI C'TyrA_G
Cuts at: 220 1135 1246 1495 2590 3505 4219 4481 220
Size: 915 111 249 1095 915 714 262 1174
Fragments arranged by size:
1174 1095 915 915 714 262 249 111
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5435
SphI G_CATG'C
Cuts at: 2122 2488 2988 2122
Size: 366 500 4569
Fragments arranged by size:
4569 500 366
SrfI GCCC'GGGC
Cuts at: 2312 2312
Size: 5435
StuI AGG'CCT
Cuts at: 1558 1836 2224 2420 1558
Size: 278 388 196 4573
Fragments arranged by size:
4573 388 278 196
StyI C'CwwG_G
Cuts at: 107 824 1790 1902 2569 2923 107
Size: 717 966 112 667 354 2619
Fragments arranged by size:
2619 966 717 667 354 112
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 145 1373 3018 3151 3610 3819 145
Size: 1228 1645 133 459 209 1761
Fragments arranged by size:
1761 1645 1228 459 209 133
TatI w'GTAC_w
Cuts at: 984 1347 4950 984
Size: 363 3603 1469
Fragments arranged by size:
3603 1469 363
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1159 1191 4952 4984 1159
Size: 32 3761 32 1610
Fragments arranged by size:
3761 1610 32 32
Tth111I GACn'n_nGTC
Cuts at: 1190 3232 1190
Size: 2042 3393
XbaI T'CTAG_A
Cuts at: 1281 2466 1281
Size: 1185 4250
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 356 1155 1788 2930 356
Size: 799 633 1142 2861
Fragments arranged by size:
2861 1142 799 633
XhoI C'TCGA_G
Cuts at: 220 220
Size: 5435
XmaI C'CCGG_G
Cuts at: 1418 2310 2455 1418
Size: 892 145 4398
Fragments arranged by size:
4398 892 145
XmnI GAAnn'nnTTC
Cuts at: 1349 1349
Size: 5435
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflII AflIII AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI BstZ17I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NspI PasI PciI PmeI PpiI PshAI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SrfI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AgeI AhdI AloI ApaI AscI AvrII
BaeI BcgI BfrBI BmgBI BmtI BsaXI BsiWI BsmBI
BspEI BssHII BstEII Bsu36I ClaI CspCI DraIII FseI
FspAI HpaI MfeI NheI NotI NruI NsiI PacI
PflMI PfoI PmlI PpuMI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SspI SwaI
ZraI