Restriction map for
pF1KB8629
(Circular) (Six-base) MAPSORT of: pF1KB8629.seq Check: 9717 from: 1 to: 4706
pF1KB8629 4706 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AccI GT'mk_AC
Cuts at: 1406 1744 1406
Size: 338 4368
Acc65I G'GTAC_C
Cuts at: 1722 1722
Size: 4706
AclI AA'CG_TT
Cuts at: 1700 4546 1700
Size: 2846 1860
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1207 1243 2531 2963 3316 3978 1207
Size: 36 1288 432 353 662 1935
Fragments arranged by size:
1935 1288 662 432 353 36
AflIII A'CryG_T
Cuts at: 363 995 1025 1031 1100 2058 3858 4139 363
Size: 632 30 6 69 958 1800 281 930
Fragments arranged by size:
1800 958 930 632 281 69 30 6
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 350 382 350
Size: 32 4674
AlwNI CAG_nnn'CTG
Cuts at: 1213 2011 3449 1213
Size: 798 1438 2470
Fragments arranged by size:
2470 1438 798
ApaLI G'TGCA_C
Cuts at: 3544 3544
Size: 4706
ApoI r'AATT_y
Cuts at: 1195 1573 1660 1710 4059 4315 1195
Size: 378 87 50 2349 256 1586
Fragments arranged by size:
2349 1586 378 256 87 50
AseI AT'TA_AT
Cuts at: 20 20
Size: 4706
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4706
AvaI C'yCGr_G
Cuts at: 1726 1726
Size: 4706
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 281 314 281
Size: 33 4673
BamHI G'GATC_C
Cuts at: 1731 1731
Size: 4706
BanI G'GyrC_C
Cuts at: 190 1136 1722 2136 2384 2419 190
Size: 946 586 414 248 35 2477
Fragments arranged by size:
2477 946 586 414 248 35
BanII G_rGCy'C
Cuts at: 1720 2750 1720
Size: 1030 3676
BbeI G_GCGC'C
Cuts at: 1140 2140 2388 1140
Size: 1000 248 3458
Fragments arranged by size:
3458 1000 248
BbsI GAAGACnn'nnnn_
Cuts at: 177 1166 1328 1426 177
Size: 989 162 98 3457
Fragments arranged by size:
3457 989 162 98
BbvCI CC'TCA_GC
Cuts at: 195 195
Size: 4706
BciVI GTATCCnnnnn_n'
Cuts at: 2604 3660 4706 2604
Size: 1056 1046 2604
Fragments arranged by size:
2604 1056 1046
BclI T'GATC_A
Cuts at: 485 2226 485
Size: 1741 2965
BglII A'GATC_T
Cuts at: 2221 4258 2221
Size: 2037 2669
BlpI GC'TnA_GC
Cuts at: 928 1818 928
Size: 890 3816
Bme1580I G_kGCm'C
Cuts at: 1025 2331 2424 3548 1025
Size: 1306 93 1124 2183
Fragments arranged by size:
2183 1306 1124 93
BmgBI CAC'GTC
Cuts at: 998 1101 998
Size: 103 4603
BmrI ACTGGGnnnn_n'
Cuts at: 1357 1918 1999 2334 4478 1357
Size: 561 81 335 2144 1585
Fragments arranged by size:
2144 1585 561 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3073 3073
Size: 4706
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 296 1366 296
Size: 1070 3636
Bpu10I CC'TnA_GC
Cuts at: 195 195
Size: 4706
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 137 1882 2761 3475 3773 137
Size: 1745 879 714 298 1070
Fragments arranged by size:
1745 1070 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4410 4410
Size: 4706
BsaAI yAC'GTr
Cuts at: 1026 2059 1026
Size: 1033 3673
BsaBI GATnn'nnATC
Cuts at: 6 1342 2220 4257 6
Size: 1336 878 2037 455
Fragments arranged by size:
2037 1336 878 455
BsaHI Gr'CG_yC
Cuts at: 1137 2137 2385 3087 4583 1137
Size: 1000 248 702 1496 1260
Fragments arranged by size:
1496 1260 1000 702 248
BsaWI w'CCGG_w
Cuts at: 1895 2416 3094 3505 3652 3926 1895
Size: 521 678 411 147 274 2675
Fragments arranged by size:
2675 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 608 608
Size: 4706
BseYI C'CCAG_C
Cuts at: 719 744 900 933 2131 2889 3554 3962 719
Size: 25 156 33 1198 758 665 408 1463
Fragments arranged by size:
1463 1198 758 665 408 156 33 25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1074 1074
Size: 4706
BsiEI CG_ry'CG
Cuts at: 104 2248 2294 3524 104
Size: 2144 46 1230 1286
Fragments arranged by size:
2144 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1720 2498 2688 3548 1720
Size: 778 190 860 2878
Fragments arranged by size:
2878 860 778 190
BsmI GAATG_Cn'
Cuts at: 1524 1524
Size: 4706
BsmBI CGTCTCn'nnnn_
Cuts at: 1134 1134
Size: 4706
Bsp1286I G_dGCh'C
Cuts at: 1025 1720 2331 2424 2498 2688 2750 3548 1025
Size: 695 611 93 74 190 62 798 2183
Fragments arranged by size:
2183 798 695 611 190 93 74 62
BspHI T'CATG_A
Cuts at: 3138 3138
Size: 4706
BspMI ACCTGCnnnn'nnnn_
Cuts at: 932 1418 1756 1970 2272 2653 932
Size: 486 338 214 302 381 2985
Fragments arranged by size:
2985 486 381 338 302 214
BsrBI CCG'CTC
Cuts at: 50 2998 3052 4536 4701 50
Size: 2948 54 1484 165 55
Fragments arranged by size:
2948 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 662 1664 2618 662
Size: 1002 954 2750
Fragments arranged by size:
2750 1002 954
BsrFI r'CCGG_y
Cuts at: 2704 2885 2704
Size: 181 4525
BsrGI T'GTAC_A
Cuts at: 1028 1028
Size: 4706
BssSI C'ACGA_G
Cuts at: 1131 2977 3685 1131
Size: 1846 708 2152
Fragments arranged by size:
2152 1846 708
BstAPI GCAn_nnn'nTGC
Cuts at: 2083 2083
Size: 4706
BstBI TT'CG_AA
Cuts at: 1230 3067 1230
Size: 1837 2869
BstYI r'GATC_y
Cuts at: 1 1731 2198 2221 2556 2802 3108 3120 3206
Size: 1730 467 23 335 246 306 12 86
Cuts at: 3206 4258 1
Size: 1052 449
Fragments arranged by size:
1730 1052 467 449 335 306 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 686 107
Size: 579 4127
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 438 2656 2837 3000 438
Size: 2218 181 163 2144
Fragments arranged by size:
2218 2144 181 163
BtsI GCAGTG_nn'
Cuts at: 1949 1949
Size: 4706
ClaI AT'CG_AT
Cuts at: 1346 1346
Size: 4706
DraI TTT'AAA
Cuts at: 1705 1705
Size: 4706
DraIII CAC_nnn'GTG
Cuts at: 804 804
Size: 4706
DrdI GACnn_nn'nnGTC
Cuts at: 2412 3756 2412
Size: 1344 3362
EaeI y'GGCC_r
Cuts at: 579 2291 2465 2856 2883 4228 579
Size: 1712 174 391 27 1345 1057
Fragments arranged by size:
1712 1345 1057 391 174 27
EagI C'GGCC_G
Cuts at: 2291 2291
Size: 4706
EarI CTCTTCn'nnn_
Cuts at: 2729 2939 2729
Size: 210 4496
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2914 3656 3802 4516 2914
Size: 742 146 714 3104
Fragments arranged by size:
3104 742 714 146
EcoICRI GAG'CTC
Cuts at: 1718 1718
Size: 4706
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 296 1207 1243 1366 2531 2963 3316 3978 296
Size: 911 36 123 1165 432 353 662 1024
Fragments arranged by size:
1165 1024 911 662 432 353 123 36
EcoO109I rG'GnC_Cy
Cuts at: 1845 1845
Size: 4706
EcoRI G'AATT_C
Cuts at: 1573 1660 1710 1573
Size: 87 50 4569
Fragments arranged by size:
4569 87 50
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2725 2725
Size: 4706
FspI TGC'GCA
Cuts at: 2487 2487
Size: 4706
HaeII r_GCGC'y
Cuts at: 1140 2140 2388 3618 4389 1140
Size: 1000 248 1230 771 1457
Fragments arranged by size:
1457 1230 1000 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 148 180 2548 2578 2580 2610 148
Size: 32 2368 30 2 30 2244
Fragments arranged by size:
2368 2244 32 30 30 2
HincII GTy'rAC
Cuts at: 1407 1745 1407
Size: 338 4368
KasI G'GCGC_C
Cuts at: 1136 2136 2384 1136
Size: 1000 248 3458
Fragments arranged by size:
3458 1000 248
KpnI G_GTAC'C
Cuts at: 1726 1726
Size: 4706
MluI A'CGCG_T
Cuts at: 4139 4139
Size: 4706
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 99 3465 3649 99
Size: 3366 184 1156
Fragments arranged by size:
3366 1156 184
MscI TGG'CCA
Cuts at: 581 2467 581
Size: 1886 2820
MslI CAynn'nnrTG
Cuts at: 946 2695 2822 946
Size: 1749 127 2830
Fragments arranged by size:
2830 1749 127
MspA1I CmG'CkG
Cuts at: 541 744 771 900 1817 2131 2491 3275 3520
Size: 203 27 129 917 314 360 784 245
Cuts at: 3520 541
Size: 1727
Fragments arranged by size:
1727 917 784 360 314 245 203 129
27
NaeI GCC'GGC
Cuts at: 2887 2887
Size: 4706
NarI GG'CG_CC
Cuts at: 1137 2137 2385 1137
Size: 1000 248 3458
Fragments arranged by size:
3458 1000 248
NcoI C'CATG_G
Cuts at: 107 686 107
Size: 579 4127
NdeI CA'TA_TG
Cuts at: 891 891
Size: 4706
NgoMIV G'CCGG_C
Cuts at: 2885 2885
Size: 4706
NspI r_CATG'y
Cuts at: 367 1035 1759 2259 3862 367
Size: 668 724 500 1603 1211
Fragments arranged by size:
1603 1211 724 668 500
PciI A'CATG_T
Cuts at: 363 1031 3858 363
Size: 668 2827 1211
Fragments arranged by size:
2827 1211 668
PmeI GTTT'AAAC
Cuts at: 1705 1705
Size: 4706
PmlI CAC'GTG
Cuts at: 1026 1026
Size: 4706
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3152 3184 3152
Size: 32 4674
PsiI TTA'TAA
Cuts at: 569 569
Size: 4706
PstI C_TGCA'G
Cuts at: 1005 1545 1626 1753 2438 1005
Size: 540 81 127 685 3273
Fragments arranged by size:
3273 685 540 127 81
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4706
PvuII CAG'CTG
Cuts at: 541 744 771 900 2131 2491 541
Size: 203 27 129 1231 360 2756
Fragments arranged by size:
2756 1231 360 203 129 27
RsrII CG'GwC_CG
Cuts at: 2901 2901
Size: 4706
SacI G_AGCT'C
Cuts at: 1720 1720
Size: 4706
SalI G'TCGA_C
Cuts at: 1405 1743 1405
Size: 338 4368
SapI GCTCTTCn'nnn_
Cuts at: 2729 2939 2729
Size: 210 4496
SbfI CC_TGCA'GG
Cuts at: 1753 1753
Size: 4706
ScaI AGT'ACT
Cuts at: 4223 4223
Size: 4706
SfcI C'TryA_G
Cuts at: 33 255 599 1001 1427 1541 1622 1749 2434
Size: 222 344 402 426 114 81 127 685
Cuts at: 2434 3402 3593 33
Size: 968 191 1146
Fragments arranged by size:
1146 968 685 426 402 344 222 191
127 114 81
SfoI GGC'GCC
Cuts at: 1138 2138 2386 1138
Size: 1000 248 3458
Fragments arranged by size:
3458 1000 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4706
SmaI CCC'GGG
Cuts at: 1728 1728
Size: 4706
SmlI C'TyrA_G
Cuts at: 152 1861 2776 3490 3752 152
Size: 1709 915 714 262 1106
Fragments arranged by size:
1709 1106 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4706
SphI G_CATG'C
Cuts at: 1759 2259 1759
Size: 500 4206
StyI C'CwwG_G
Cuts at: 107 447 686 1840 2194 107
Size: 340 239 1154 354 2619
Fragments arranged by size:
2619 1154 354 340 239
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1093 2289 2422 2881 3090 1093
Size: 1196 133 459 209 2709
Fragments arranged by size:
2709 1196 459 209 133
TatI w'GTAC_w
Cuts at: 289 1028 4221 289
Size: 739 3193 774
Fragments arranged by size:
3193 774 739
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 165 197 1496 1528 4223 4255 165
Size: 32 1299 32 2695 32 616
Fragments arranged by size:
2695 1299 616 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1402 2503 1402
Size: 1101 3605
XbaI T'CTAG_A
Cuts at: 1737 1737
Size: 4706
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 430 693 2201 430
Size: 263 1508 2935
Fragments arranged by size:
2935 1508 263
XmaI C'CCGG_G
Cuts at: 1726 1726
Size: 4706
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AloI AlwNI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
ClaI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NspI PciI PmeI
PmlI PpiI PsiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
ApaI AscI AvrII BcgI BfrBI BglI BmtI BsaXI
BsiWI BspEI BssHII BstEII BstXI BstZ17I Bsu36I CspCI
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PpuMI
PshAI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI