Restriction map for
pF1KB8630
(Circular) (Six-base) MAPSORT of: pF1KB8630.seq Check: 2385 from: 1 to: 5621
pF1KB8630 5621 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AccI GT'mk_AC
Cuts at: 1106 1991 2659 1106
Size: 885 668 4068
Fragments arranged by size:
4068 885 668
Acc65I G'GTAC_C
Cuts at: 2637 2637
Size: 5621
AclI AA'CG_TT
Cuts at: 5461 5461
Size: 5621
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1636 1658 3446 3878 4231 4893 1636
Size: 22 1788 432 353 662 2364
Fragments arranged by size:
2364 1788 662 432 353 22
AfeI AGC'GCT
Cuts at: 132 132
Size: 5621
AflIII A'CryG_T
Cuts at: 2973 4773 5054 2973
Size: 1800 281 3540
Fragments arranged by size:
3540 1800 281
AgeI A'CCGG_T
Cuts at: 1807 1807
Size: 5621
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 665 697 1251 1283 665
Size: 32 554 32 5003
Fragments arranged by size:
5003 554 32 32
AlwNI CAG_nnn'CTG
Cuts at: 403 611 2926 4364 403
Size: 208 2315 1438 1660
Fragments arranged by size:
2315 1660 1438 208
ApaI G_GGCC'C
Cuts at: 2523 2523
Size: 5621
ApaLI G'TGCA_C
Cuts at: 4459 4459
Size: 5621
ApoI r'AATT_y
Cuts at: 1631 2625 4974 5230 1631
Size: 994 2349 256 2022
Fragments arranged by size:
2349 2022 994 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5621
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5621
AvaI C'yCGr_G
Cuts at: 1098 2641 1098
Size: 1543 4078
AvrII C'CTAG_G
Cuts at: 1941 1941
Size: 5621
BamHI G'GATC_C
Cuts at: 2226 2646 2226
Size: 420 5201
BanI G'GyrC_C
Cuts at: 826 2637 3051 3299 3334 826
Size: 1811 414 248 35 3113
Fragments arranged by size:
3113 1811 414 248 35
BanII G_rGCy'C
Cuts at: 143 1099 1431 1918 2523 2635 3665 143
Size: 956 332 487 605 112 1030 2099
Fragments arranged by size:
2099 1030 956 605 487 332 112
BbeI G_GCGC'C
Cuts at: 3055 3303 3055
Size: 248 5373
BbsI GAAGACnn'nnnn_
Cuts at: 2343 2343
Size: 5621
BbvCI CC'TCA_GC
Cuts at: 2428 2428
Size: 5621
BciVI GTATCCnnnnn_n'
Cuts at: 439 3519 4575 5621 439
Size: 3080 1056 1046 439
Fragments arranged by size:
3080 1056 1046 439
BclI T'GATC_A
Cuts at: 3141 3141
Size: 5621
BfrBI ATG'CAT
Cuts at: 2253 2253
Size: 5621
BglII A'GATC_T
Cuts at: 3136 5173 3136
Size: 2037 3584
BlpI GC'TnA_GC
Cuts at: 2733 2733
Size: 5621
Bme1580I G_kGCm'C
Cuts at: 831 2523 3246 3339 4463 831
Size: 1692 723 93 1124 1989
Fragments arranged by size:
1989 1692 1124 723 93
BmrI ACTGGGnnnn_n'
Cuts at: 2833 2914 3249 5393 2833
Size: 81 335 2144 3061
Fragments arranged by size:
3061 2144 335 81
BmtI G_CTAG'C
Cuts at: 2170 2384 2170
Size: 214 5407
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 149 3988 149
Size: 3839 1782
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 381 1050 1702 2476 381
Size: 669 652 774 3526
Fragments arranged by size:
3526 774 669 652
Bpu10I CC'TnA_GC
Cuts at: 784 2428 784
Size: 1644 3977
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1444 1495 2515 2797 3676 4390 4688 1444
Size: 51 1020 282 879 714 298 2377
Fragments arranged by size:
2377 1020 879 714 298 282 51
BsaI GGTCTCn'nnnn_
Cuts at: 412 5325 412
Size: 4913 708
BsaAI yAC'GTr
Cuts at: 2974 2974
Size: 5621
BsaBI GATnn'nnATC
Cuts at: 6 3135 5172 6
Size: 3129 2037 455
Fragments arranged by size:
3129 2037 455
BsaHI Gr'CG_yC
Cuts at: 3052 3300 4002 5498 3052
Size: 248 702 1496 3175
Fragments arranged by size:
3175 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1807 2810 3331 4009 4420 4567 4841 1807
Size: 1003 521 678 411 147 274 2587
Fragments arranged by size:
2587 1003 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 665 695 1055 1085 665
Size: 30 360 30 5201
Fragments arranged by size:
5201 360 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 544 895 544
Size: 351 5270
BseYI C'CCAG_C
Cuts at: 1262 1581 3046 3804 4469 4877 1262
Size: 319 1465 758 665 408 2006
Fragments arranged by size:
2006 1465 758 665 408 319
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 297 2458 297
Size: 2161 3460
BsiEI CG_ry'CG
Cuts at: 104 3163 3209 4439 104
Size: 3059 46 1230 1286
Fragments arranged by size:
3059 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1918 2635 3413 3603 4463 1918
Size: 717 778 190 860 3076
Fragments arranged by size:
3076 860 778 717 190
BsmI GAATG_Cn'
Cuts at: 1120 1155 1120
Size: 35 5586
BsmBI CGTCTCn'nnnn_
Cuts at: 2290 2290
Size: 5621
Bsp1286I G_dGCh'C
Cuts at: 143 831 1099 1431 1918 2523 2635 3246 3339
Size: 688 268 332 487 605 112 611 93
Cuts at: 3339 3413 3603 3665 4463 143
Size: 74 190 62 798 1301
Fragments arranged by size:
1301 798 688 611 605 487 332 268
190 112 93 74 62
BspHI T'CATG_A
Cuts at: 1956 4053 1956
Size: 2097 3524
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1721 2522 2671 2885 3187 3568 1721
Size: 801 149 214 302 381 3774
Fragments arranged by size:
3774 801 381 302 214 149
BsrBI CCG'CTC
Cuts at: 50 3913 3967 5451 5616 50
Size: 3863 54 1484 165 55
Fragments arranged by size:
3863 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1125 3533 1125
Size: 2408 3213
BsrFI r'CCGG_y
Cuts at: 302 1807 3619 3800 302
Size: 1505 1812 181 2123
Fragments arranged by size:
2123 1812 1505 181
BsrGI T'GTAC_A
Cuts at: 1934 2042 1934
Size: 108 5513
BssSI C'ACGA_G
Cuts at: 3892 4600 3892
Size: 708 4913
BstAPI GCAn_nnn'nTGC
Cuts at: 2998 2998
Size: 5621
BstBI TT'CG_AA
Cuts at: 3982 3982
Size: 5621
BstEII G'GTnAC_C
Cuts at: 1597 1597
Size: 5621
BstXI CCAn_nnnn'nTGG
Cuts at: 343 343
Size: 5621
BstYI r'GATC_y
Cuts at: 1 428 1874 2119 2226 2646 3113 3136 3471
Size: 427 1446 245 107 420 467 23 335
Cuts at: 3471 3717 4023 4035 4121 5173 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1446 1052 467 449 427 420 335 306
246 245 107 86 23 12
BstZ17I GTA'TAC
Cuts at: 1992 1992
Size: 5621
BtgI C'CryG_G
Cuts at: 232 232
Size: 5621
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3571 3752 3915 3571
Size: 181 163 5277
Fragments arranged by size:
5277 181 163
BtsI GCAGTG_nn'
Cuts at: 509 2864 509
Size: 2355 3266
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2021 2056 2021
Size: 35 5586
DraI TTT'AAA
Cuts at: 1802 2620 1802
Size: 818 4803
DraIII CAC_nnn'GTG
Cuts at: 1047 1047
Size: 5621
DrdI GACnn_nn'nnGTC
Cuts at: 3327 4671 3327
Size: 1344 4277
EaeI y'GGCC_r
Cuts at: 304 1763 3206 3380 3771 3798 5143 304
Size: 1459 1443 174 391 27 1345 782
Fragments arranged by size:
1459 1443 1345 782 391 174 27
EagI C'GGCC_G
Cuts at: 3206 3206
Size: 5621
EarI CTCTTCn'nnn_
Cuts at: 275 415 550 1087 1612 2209 3644 3854 275
Size: 140 135 537 525 597 1435 210 2042
Fragments arranged by size:
2042 1435 597 537 525 210 140 135
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3829 4571 4717 5431 3829
Size: 742 146 714 4019
Fragments arranged by size:
4019 742 714 146
EcoICRI GAG'CTC
Cuts at: 1916 2633 1916
Size: 717 4904
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 381 1050 1636 1658 1702 2476 3446 3878 4231
Size: 669 586 22 44 774 970 432 353
Cuts at: 4231 4893 381
Size: 662 1109
Fragments arranged by size:
1109 970 774 669 662 586 432 353
44 22
EcoO109I rG'GnC_Cy
Cuts at: 213 1896 2520 2760 213
Size: 1683 624 240 3074
Fragments arranged by size:
3074 1683 624 240
EcoRI G'AATT_C
Cuts at: 2625 2625
Size: 5621
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1227 2223 3640 1227
Size: 996 1417 3208
Fragments arranged by size:
3208 1417 996
FspI TGC'GCA
Cuts at: 642 3402 642
Size: 2760 2861
HaeII r_GCGC'y
Cuts at: 134 1652 3055 3303 4533 5304 134
Size: 1518 1403 248 1230 771 451
Fragments arranged by size:
1518 1403 1230 771 451 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 354 386 515 547 1055 1087 1189 1221 3463
Size: 32 129 32 508 32 102 32 2242
Cuts at: 3463 3493 3495 3525 354
Size: 30 2 30 2450
Fragments arranged by size:
2450 2242 508 129 102 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 2660 2660
Size: 5621
HindIII A'AGCT_T
Cuts at: 417 861 417
Size: 444 5177
KasI G'GCGC_C
Cuts at: 3051 3299 3051
Size: 248 5373
KpnI G_GTAC'C
Cuts at: 2641 2641
Size: 5621
MluI A'CGCG_T
Cuts at: 5054 5054
Size: 5621
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 893 4380 4564 893
Size: 3487 184 1950
Fragments arranged by size:
3487 1950 184
MscI TGG'CCA
Cuts at: 1765 3382 1765
Size: 1617 4004
MslI CAynn'nnrTG
Cuts at: 2480 3610 3737 2480
Size: 1130 127 4364
Fragments arranged by size:
4364 1130 127
MspA1I CmG'CkG
Cuts at: 886 2299 2320 2398 2732 3046 3406 4190 4435
Size: 1413 21 78 334 314 360 784 245
Cuts at: 4435 886
Size: 2072
Fragments arranged by size:
2072 1413 784 360 334 314 245 78
21
NaeI GCC'GGC
Cuts at: 3802 3802
Size: 5621
NarI GG'CG_CC
Cuts at: 3052 3300 3052
Size: 248 5373
NcoI C'CATG_G
Cuts at: 232 232
Size: 5621
NgoMIV G'CCGG_C
Cuts at: 3800 3800
Size: 5621
NheI G'CTAG_C
Cuts at: 2166 2380 2166
Size: 214 5407
NsiI A_TGCA'T
Cuts at: 2255 2255
Size: 5621
NspI r_CATG'y
Cuts at: 394 657 1695 2253 2370 2674 3174 4777 394
Size: 263 1038 558 117 304 500 1603 1238
Fragments arranged by size:
1603 1238 1038 558 500 304 263 117
PciI A'CATG_T
Cuts at: 4773 4773
Size: 5621
PflMI CCAn_nnn'nTGG
Cuts at: 611 611
Size: 5621
PfoI T'CCnGG_A
Cuts at: 1718 1718
Size: 5621
PmeI GTTT'AAAC
Cuts at: 2620 2620
Size: 5621
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 665 697 4067 4099 665
Size: 32 3370 32 2187
Fragments arranged by size:
3370 2187 32 32
PpuMI rG'GwC_Cy
Cuts at: 213 1896 213
Size: 1683 3938
PsiI TTA'TAA
Cuts at: 1700 1757 1700
Size: 57 5564
PspOMI G'GGCC_C
Cuts at: 2519 2519
Size: 5621
PstI C_TGCA'G
Cuts at: 2528 2668 3353 2528
Size: 140 685 4796
Fragments arranged by size:
4796 685 140
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5621
PvuII CAG'CTG
Cuts at: 2299 2320 2398 3046 3406 2299
Size: 21 78 648 360 4514
Fragments arranged by size:
4514 648 360 78 21
RsrII CG'GwC_CG
Cuts at: 3816 3816
Size: 5621
SacI G_AGCT'C
Cuts at: 1918 2635 1918
Size: 717 4904
SalI G'TCGA_C
Cuts at: 2658 2658
Size: 5621
SapI GCTCTTCn'nnn_
Cuts at: 1087 2209 3644 3854 1087
Size: 1122 1435 210 2854
Fragments arranged by size:
2854 1435 1122 210
SbfI CC_TGCA'GG
Cuts at: 2668 2668
Size: 5621
ScaI AGT'ACT
Cuts at: 5138 5138
Size: 5621
SfcI C'TryA_G
Cuts at: 33 1481 1653 2524 2664 3349 4317 4508 33
Size: 1448 172 871 140 685 968 191 1146
Fragments arranged by size:
1448 1146 968 871 685 191 172 140
SfoI GGC'GCC
Cuts at: 3053 3301 3053
Size: 248 5373
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5621
SmaI CCC'GGG
Cuts at: 2643 2643
Size: 5621
SmlI C'TyrA_G
Cuts at: 1459 1510 2530 2776 3691 4405 4667 1459
Size: 51 1020 246 915 714 262 2413
Fragments arranged by size:
2413 1020 915 714 262 246 51
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5621
SphI G_CATG'C
Cuts at: 394 2674 3174 394
Size: 2280 500 2841
Fragments arranged by size:
2841 2280 500
StuI AGG'CCT
Cuts at: 1779 2433 1779
Size: 654 4967
StyI C'CwwG_G
Cuts at: 232 435 1602 1827 1941 2017 2755 3109 232
Size: 203 1167 225 114 76 738 354 2744
Fragments arranged by size:
2744 1167 738 354 225 203 114 76
SwaI ATTT'AAAT
Cuts at: 1802 1802
Size: 5621
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 814 1408 2044 3204 3337 3796 4005 814
Size: 594 636 1160 133 459 209 2430
Fragments arranged by size:
2430 1160 636 594 459 209 133
TatI w'GTAC_w
Cuts at: 1667 1934 2042 5136 1667
Size: 267 108 3094 2152
Fragments arranged by size:
3094 2152 267 108
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5138 5170 5138
Size: 32 5589
Tth111I GACn'n_nGTC
Cuts at: 1203 2542 3418 1203
Size: 1339 876 3406
Fragments arranged by size:
3406 1339 876
XbaI T'CTAG_A
Cuts at: 2652 2652
Size: 5621
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 230 1589 3116 230
Size: 1359 1527 2735
Fragments arranged by size:
2735 1527 1359
XmaI C'CCGG_G
Cuts at: 2641 2641
Size: 5621
XmnI GAAnn'nnTTC
Cuts at: 2003 2003
Size: 5621
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BfrBI BglII BlpI Bme1580I BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NheI NsiI NspI
PciI PflMI PfoI PmeI PpiI PpuMI PsiI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI SwaI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AscI BaeI
BcgI BglI BmgBI BsiWI BspEI BssHII Bsu36I ClaI
EcoNI EcoRV FseI FspAI HpaI MfeI NdeI NotI
NruI PacI PasI PmlI PshAI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI XhoI
ZraI