Restriction map for
pF1KB8632
(Circular) (Six-base) MAPSORT of: pF1KB8632.seq Check: 1257 from: 1 to: 5627
pF1KB8632 5627 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AccI GT'mk_AC
Cuts at: 2665 2665
Size: 5627
Acc65I G'GTAC_C
Cuts at: 2643 2643
Size: 5627
AclI AA'CG_TT
Cuts at: 5467 5467
Size: 5627
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 536 1118 1301 1457 1723 1913 3452 3884 4237
Size: 582 183 156 266 190 1539 432 353
Cuts at: 4237 4899 536
Size: 662 1264
Fragments arranged by size:
1539 1264 662 582 432 353 266 190
183 156
AflIII A'CryG_T
Cuts at: 2979 4779 5060 2979
Size: 1800 281 3546
Fragments arranged by size:
3546 1800 281
AgeI A'CCGG_T
Cuts at: 1790 1790
Size: 5627
AlwNI CAG_nnn'CTG
Cuts at: 1345 1843 2932 4370 1345
Size: 498 1089 1438 2602
Fragments arranged by size:
2602 1438 1089 498
ApaI G_GGCC'C
Cuts at: 498 1206 498
Size: 708 4919
ApaLI G'TGCA_C
Cuts at: 4465 4465
Size: 5627
ApoI r'AATT_y
Cuts at: 505 1597 1652 1805 1906 1928 2337 2548 2631
Size: 1092 55 153 101 22 409 211 83
Cuts at: 2631 4980 5236 505
Size: 2349 256 896
Fragments arranged by size:
2349 1092 896 409 256 211 153 101
83 55 22
AseI AT'TA_AT
Cuts at: 20 1918 20
Size: 1898 3729
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5627
AvaI C'yCGr_G
Cuts at: 1997 2647 1997
Size: 650 4977
BamHI G'GATC_C
Cuts at: 1366 1935 2652 1366
Size: 569 717 4341
Fragments arranged by size:
4341 717 569
BanI G'GyrC_C
Cuts at: 661 2643 3057 3305 3340 661
Size: 1982 414 248 35 2948
Fragments arranged by size:
2948 1982 414 248 35
BanII G_rGCy'C
Cuts at: 498 1206 2641 3671 498
Size: 708 1435 1030 2454
Fragments arranged by size:
2454 1435 1030 708
BbeI G_GCGC'C
Cuts at: 3061 3309 3061
Size: 248 5379
BbsI GAAGACnn'nnnn_
Cuts at: 800 800
Size: 5627
BbvCI CC'TCA_GC
Cuts at: 1382 1544 1382
Size: 162 5465
BciVI GTATCCnnnnn_n'
Cuts at: 3525 4581 5627 3525
Size: 1056 1046 3525
Fragments arranged by size:
3525 1056 1046
BclI T'GATC_A
Cuts at: 1746 3147 1746
Size: 1401 4226
BfrBI ATG'CAT
Cuts at: 1558 1558
Size: 5627
BglII A'GATC_T
Cuts at: 3142 5179 3142
Size: 2037 3590
BlpI GC'TnA_GC
Cuts at: 2564 2739 2564
Size: 175 5452
Bme1580I G_kGCm'C
Cuts at: 138 498 1206 3252 3345 4469 138
Size: 360 708 2046 93 1124 1296
Fragments arranged by size:
2046 1296 1124 708 360 93
BmrI ACTGGGnnnn_n'
Cuts at: 122 254 1386 2839 2920 3255 5399 122
Size: 132 1132 1453 81 335 2144 350
Fragments arranged by size:
2144 1453 1132 350 335 132 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 777 3994 777
Size: 3217 2410
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 482 842 1154 1548 482
Size: 360 312 394 4561
Fragments arranged by size:
4561 394 360 312
Bpu10I CC'TnA_GC
Cuts at: 1060 1382 1544 1060
Size: 322 162 5143
Fragments arranged by size:
5143 322 162
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 362 1699 2209 2542 2803 3682 4396 4694 362
Size: 1337 510 333 261 879 714 298 1295
Fragments arranged by size:
1337 1295 879 714 510 333 298 261
BsaI GGTCTCn'nnnn_
Cuts at: 259 787 2410 5331 259
Size: 528 1623 2921 555
Fragments arranged by size:
2921 1623 555 528
BsaAI yAC'GTr
Cuts at: 2980 2980
Size: 5627
BsaBI GATnn'nnATC
Cuts at: 6 3141 5178 6
Size: 3135 2037 455
Fragments arranged by size:
3135 2037 455
BsaHI Gr'CG_yC
Cuts at: 3058 3306 4008 5504 3058
Size: 248 702 1496 3181
Fragments arranged by size:
3181 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1790 2816 3337 4015 4426 4573 4847 1790
Size: 1026 521 678 411 147 274 2570
Fragments arranged by size:
2570 1026 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 967 997 967
Size: 30 5597
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 721 769 1051 1267 1363 1561 2569 721
Size: 48 282 216 96 198 1008 3779
Fragments arranged by size:
3779 1008 282 216 198 96 48
BseYI C'CCAG_C
Cuts at: 160 178 3052 3810 4475 4883 160
Size: 18 2874 758 665 408 904
Fragments arranged by size:
2874 904 758 665 408 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1856 1856
Size: 5627
BsiEI CG_ry'CG
Cuts at: 104 3169 3215 4445 104
Size: 3065 46 1230 1286
Fragments arranged by size:
3065 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 2641 3419 3609 4469 2641
Size: 778 190 860 3799
Fragments arranged by size:
3799 860 778 190
Bsp1286I G_dGCh'C
Cuts at: 138 498 1206 2641 3252 3345 3419 3609 3671
Size: 360 708 1435 611 93 74 190 62
Cuts at: 3671 4469 138
Size: 798 1296
Fragments arranged by size:
1435 1296 798 708 611 360 190 93
74 62
BspHI T'CATG_A
Cuts at: 1947 4059 1947
Size: 2112 3515
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2677 2891 3193 3574 2677
Size: 214 302 381 4730
Fragments arranged by size:
4730 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3919 3973 5457 5622 50
Size: 3869 54 1484 165 55
Fragments arranged by size:
3869 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 317 1973 3539 317
Size: 1656 1566 2405
Fragments arranged by size:
2405 1656 1566
BsrFI r'CCGG_y
Cuts at: 1790 3625 3806 1790
Size: 1835 181 3611
Fragments arranged by size:
3611 1835 181
BsrGI T'GTAC_A
Cuts at: 1511 1511
Size: 5627
BssSI C'ACGA_G
Cuts at: 3898 4606 3898
Size: 708 4919
BstAPI GCAn_nnn'nTGC
Cuts at: 3004 3004
Size: 5627
BstBI TT'CG_AA
Cuts at: 3988 3988
Size: 5627
BstXI CCAn_nnnn'nTGG
Cuts at: 1698 1698
Size: 5627
BstYI r'GATC_y
Cuts at: 1 919 955 1176 1366 1935 2652 3119 3142
Size: 918 36 221 190 569 717 467 23
Cuts at: 3142 3477 3723 4029 4041 4127 5179 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
1052 918 717 569 467 449 335 306
246 221 190 86 36 23 12
Bsu36I CC'TnA_GG
Cuts at: 2603 2603
Size: 5627
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3577 3758 3921 3577
Size: 181 163 5283
Fragments arranged by size:
5283 181 163
BtsI GCAGTG_nn'
Cuts at: 527 575 2870 527
Size: 48 2295 3284
Fragments arranged by size:
3284 2295 48
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2381 2416 2381
Size: 35 5592
DraI TTT'AAA
Cuts at: 2626 2626
Size: 5627
DraIII CAC_nnn'GTG
Cuts at: 2176 2176
Size: 5627
DrdI GACnn_nn'nnGTC
Cuts at: 3333 4677 3333
Size: 1344 4283
EaeI y'GGCC_r
Cuts at: 565 572 1409 3212 3386 3777 3804 5149 565
Size: 7 837 1803 174 391 27 1345 1043
Fragments arranged by size:
1803 1345 1043 837 391 174 27 7
EagI C'GGCC_G
Cuts at: 3212 3212
Size: 5627
EarI CTCTTCn'nnn_
Cuts at: 161 821 851 1133 1412 1436 1655 1715 1889
Size: 660 30 282 279 24 219 60 174
Cuts at: 1889 2414 3650 3860 161
Size: 525 1236 210 1928
Fragments arranged by size:
1928 1236 660 525 282 279 219 210
174 60 30 24
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1379 3835 4577 4723 5437 1379
Size: 2456 742 146 714 1569
Fragments arranged by size:
2456 1569 742 714 146
EcoICRI GAG'CTC
Cuts at: 2639 2639
Size: 5627
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 482 536 842 1118 1154 1301 1457 1548 1723
Size: 54 306 276 36 147 156 91 175
Cuts at: 1723 1913 3452 3884 4237 4899 482
Size: 190 1539 432 353 662 1210
Fragments arranged by size:
1539 1210 662 432 353 306 276 190
175 156 147 91 54 36
EcoO109I rG'GnC_Cy
Cuts at: 230 609 1202 1271 1457 1568 2766 230
Size: 379 593 69 186 111 1198 3091
Fragments arranged by size:
3091 1198 593 379 186 111 69
EcoRI G'AATT_C
Cuts at: 1597 1652 1906 2631 1597
Size: 55 254 725 4593
Fragments arranged by size:
4593 725 254 55
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1927 2514 3646 1927
Size: 587 1132 3908
Fragments arranged by size:
3908 1132 587
FspI TGC'GCA
Cuts at: 3408 3408
Size: 5627
HaeII r_GCGC'y
Cuts at: 431 3061 3309 4539 5310 431
Size: 2630 248 1230 771 748
Fragments arranged by size:
2630 1230 771 748 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 256 288 574 606 617 649 781 813 934
Size: 32 286 32 11 32 132 32 121
Cuts at: 934 966 1375 1407 1714 1746 1837 1869 3469
Size: 32 409 32 307 32 91 32 1600
Cuts at: 3469 3499 3501 3531 256
Size: 30 2 30 2352
Fragments arranged by size:
2352 1600 409 307 286 132 121 91
32 32 32 32 32 32 32 32
30 30 11 2
HincII GTy'rAC
Cuts at: 1218 2666 1218
Size: 1448 4179
KasI G'GCGC_C
Cuts at: 3057 3305 3057
Size: 248 5379
KpnI G_GTAC'C
Cuts at: 2647 2647
Size: 5627
MluI A'CGCG_T
Cuts at: 5060 5060
Size: 5627
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 975 4386 4570 975
Size: 3411 184 2032
Fragments arranged by size:
3411 2032 184
MscI TGG'CCA
Cuts at: 567 574 1411 3388 567
Size: 7 837 1977 2806
Fragments arranged by size:
2806 1977 837 7
MslI CAynn'nnrTG
Cuts at: 2142 3616 3743 2142
Size: 1474 127 4026
Fragments arranged by size:
4026 1474 127
MspA1I CmG'CkG
Cuts at: 1543 1862 2196 2738 3052 3412 4196 4441 1543
Size: 319 334 542 314 360 784 245 2729
Fragments arranged by size:
2729 784 542 360 334 319 314 245
NaeI GCC'GGC
Cuts at: 3808 3808
Size: 5627
NarI GG'CG_CC
Cuts at: 3058 3306 3058
Size: 248 5379
NdeI CA'TA_TG
Cuts at: 2143 2143
Size: 5627
NgoMIV G'CCGG_C
Cuts at: 3806 3806
Size: 5627
NsiI A_TGCA'T
Cuts at: 1560 1560
Size: 5627
NspI r_CATG'y
Cuts at: 2680 3180 4783 2680
Size: 500 1603 3524
Fragments arranged by size:
3524 1603 500
PasI CC'CwG_GG
Cuts at: 666 743 851 666
Size: 77 108 5442
Fragments arranged by size:
5442 108 77
PciI A'CATG_T
Cuts at: 4779 4779
Size: 5627
PflMI CCAn_nnn'nTGG
Cuts at: 348 348
Size: 5627
PfoI T'CCnGG_A
Cuts at: 1799 1799
Size: 5627
PmeI GTTT'AAAC
Cuts at: 2626 2626
Size: 5627
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 760 792 4073 4105 760
Size: 32 3281 32 2282
Fragments arranged by size:
3281 2282 32 32
PpuMI rG'GwC_Cy
Cuts at: 230 1271 1457 230
Size: 1041 186 4400
Fragments arranged by size:
4400 1041 186
PshAI GACnn'nnGTC
Cuts at: 835 835
Size: 5627
PsiI TTA'TAA
Cuts at: 2479 2479
Size: 5627
PspOMI G'GGCC_C
Cuts at: 494 1202 494
Size: 708 4919
PstI C_TGCA'G
Cuts at: 1861 2674 3359 1861
Size: 813 685 4129
Fragments arranged by size:
4129 813 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5627
PvuII CAG'CTG
Cuts at: 1543 1862 2196 3052 3412 1543
Size: 319 334 856 360 3758
Fragments arranged by size:
3758 856 360 334 319
RsrII CG'GwC_CG
Cuts at: 3822 3822
Size: 5627
SacI G_AGCT'C
Cuts at: 2641 2641
Size: 5627
SalI G'TCGA_C
Cuts at: 2664 2664
Size: 5627
SapI GCTCTTCn'nnn_
Cuts at: 1715 3650 3860 1715
Size: 1935 210 3482
Fragments arranged by size:
3482 1935 210
SbfI CC_TGCA'GG
Cuts at: 2674 2674
Size: 5627
ScaI AGT'ACT
Cuts at: 5144 5144
Size: 5627
SexAI A'CCwGG_T
Cuts at: 868 1605 868
Size: 737 4890
SfcI C'TryA_G
Cuts at: 33 800 1857 2670 3355 4323 4514 33
Size: 767 1057 813 685 968 191 1146
Fragments arranged by size:
1146 1057 968 813 767 685 191
SfoI GGC'GCC
Cuts at: 3059 3307 3059
Size: 248 5379
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5627
SmaI CCC'GGG
Cuts at: 2649 2649
Size: 5627
SmlI C'TyrA_G
Cuts at: 377 1678 2188 2521 2782 3697 4411 4673 377
Size: 1301 510 333 261 915 714 262 1331
Fragments arranged by size:
1331 1301 915 714 510 333 262 261
SpeI A'CTAG_T
Cuts at: 67 630 67
Size: 563 5064
SphI G_CATG'C
Cuts at: 2680 3180 2680
Size: 500 5127
SspI AAT'ATT
Cuts at: 2396 2436 2396
Size: 40 5587
StuI AGG'CCT
Cuts at: 760 1483 760
Size: 723 4904
StyI C'CwwG_G
Cuts at: 221 449 473 2761 3115 221
Size: 228 24 2288 354 2733
Fragments arranged by size:
2733 2288 354 228 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 340 679 3210 3343 3802 4011 340
Size: 339 2531 133 459 209 1956
Fragments arranged by size:
2531 1956 459 339 209 133
TatI w'GTAC_w
Cuts at: 1511 5142 1511
Size: 3631 1996
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5144 5176 5144
Size: 32 5595
Tth111I GACn'n_nGTC
Cuts at: 270 1585 3424 270
Size: 1315 1839 2473
Fragments arranged by size:
2473 1839 1315
XbaI T'CTAG_A
Cuts at: 2062 2658 2062
Size: 596 5031
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 241 313 349 505 691 986 3122 241
Size: 72 36 156 186 295 2136 2746
Fragments arranged by size:
2746 2136 295 186 156 72 36
XmaI C'CCGG_G
Cuts at: 2647 2647
Size: 5627
XmnI GAAnn'nnTTC
Cuts at: 653 1983 653
Size: 1330 4297
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgZI BtsI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NdeI NgoMIV NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BcgI BglI BmgBI BmtI BsiWI
BsmI BsmBI BspEI BssHII BstEII BstZ17I BtgI ClaI
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NcoI
NheI NotI NruI PacI PmlI PspXI PsrI SacII
SanDI SfiI SgrAI SnaBI SrfI SwaI XhoI ZraI