Restriction map for
pF1KB8633
(Circular) (Six-base) MAPSORT of: pF1KB8633.seq Check: 6889 from: 1 to: 4619
pF1KB8633 4619 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AccI GT'mk_AC
Cuts at: 1657 1657
Size: 4619
Acc65I G'GTAC_C
Cuts at: 1281 1635 1281
Size: 354 4265
AclI AA'CG_TT
Cuts at: 4459 4459
Size: 4619
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 559 2444 2876 3229 3891 559
Size: 1885 432 353 662 1287
Fragments arranged by size:
1885 1287 662 432 353
AfeI AGC'GCT
Cuts at: 1048 1048
Size: 4619
AflIII A'CryG_T
Cuts at: 260 1971 3771 4052 260
Size: 1711 1800 281 827
Fragments arranged by size:
1800 1711 827 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 545 545
Size: 4619
AlwNI CAG_nnn'CTG
Cuts at: 1210 1924 3362 1210
Size: 714 1438 2467
Fragments arranged by size:
2467 1438 714
ApaLI G'TGCA_C
Cuts at: 3457 3457
Size: 4619
ApoI r'AATT_y
Cuts at: 649 1623 3972 4228 649
Size: 974 2349 256 1040
Fragments arranged by size:
2349 1040 974 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4619
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4619
AvaI C'yCGr_G
Cuts at: 1639 1639
Size: 4619
BamHI G'GATC_C
Cuts at: 1644 1644
Size: 4619
BanI G'GyrC_C
Cuts at: 114 1059 1281 1391 1635 2049 2297 2332 114
Size: 945 222 110 244 414 248 35 2401
Fragments arranged by size:
2401 945 414 248 244 222 110 35
BanII G_rGCy'C
Cuts at: 950 1141 1317 1633 2663 950
Size: 191 176 316 1030 2906
Fragments arranged by size:
2906 1030 316 191 176
BbeI G_GCGC'C
Cuts at: 118 2053 2301 118
Size: 1935 248 2436
Fragments arranged by size:
2436 1935 248
BbsI GAAGACnn'nnnn_
Cuts at: 745 746 745
Size: 1 4618
BciVI GTATCCnnnnn_n'
Cuts at: 2517 3573 4619 2517
Size: 1056 1046 2517
Fragments arranged by size:
2517 1056 1046
BclI T'GATC_A
Cuts at: 783 2139 783
Size: 1356 3263
BfrBI ATG'CAT
Cuts at: 994 994
Size: 4619
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 4619
BglII A'GATC_T
Cuts at: 2134 4171 2134
Size: 2037 2582
BlpI GC'TnA_GC
Cuts at: 533 1731 533
Size: 1198 3421
Bme1580I G_kGCm'C
Cuts at: 1396 2244 2337 3461 1396
Size: 848 93 1124 2554
Fragments arranged by size:
2554 1124 848 93
BmgBI CAC'GTC
Cuts at: 263 843 263
Size: 580 4039
BmrI ACTGGGnnnn_n'
Cuts at: 270 1231 1831 1912 2247 4391 270
Size: 961 600 81 335 2144 498
Fragments arranged by size:
2144 961 600 498 335 81
BmtI G_CTAG'C
Cuts at: 1217 1217
Size: 4619
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1336 2986 1336
Size: 1650 2969
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 673 1297 1314 673
Size: 624 17 3978
Fragments arranged by size:
3978 624 17
Bpu10I CC'TnA_GC
Cuts at: 1199 1199
Size: 4619
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 436 1051 1795 2674 3388 3686 436
Size: 615 744 879 714 298 1369
Fragments arranged by size:
1369 879 744 714 615 298
BsaI GGTCTCn'nnnn_
Cuts at: 4323 4323
Size: 4619
BsaAI yAC'GTr
Cuts at: 1972 1972
Size: 4619
BsaBI GATnn'nnATC
Cuts at: 6 2133 4170 6
Size: 2127 2037 455
Fragments arranged by size:
2127 2037 455
BsaHI Gr'CG_yC
Cuts at: 115 2050 2298 3000 4496 115
Size: 1935 248 702 1496 238
Fragments arranged by size:
1935 1496 702 248 238
BsaWI w'CCGG_w
Cuts at: 1808 2329 3007 3418 3565 3839 1808
Size: 521 678 411 147 274 2588
Fragments arranged by size:
2588 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1061 1317 1061
Size: 256 4363
BseYI C'CCAG_C
Cuts at: 425 872 1169 1464 2044 2802 3467 3875 425
Size: 447 297 295 580 758 665 408 1169
Fragments arranged by size:
1169 758 665 580 447 408 297 295
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 185 185
Size: 4619
BsiEI CG_ry'CG
Cuts at: 104 2161 2207 3437 104
Size: 2057 46 1230 1286
Fragments arranged by size:
2057 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 460 1317 1609 1633 2411 2601 3461 460
Size: 857 292 24 778 190 860 1618
Fragments arranged by size:
1618 860 857 778 292 190 24
Bsp1286I G_dGCh'C
Cuts at: 460 950 1141 1317 1396 1609 1633 2244 2337
Size: 490 191 176 79 213 24 611 93
Cuts at: 2337 2411 2601 2663 3461 460
Size: 74 190 62 798 1618
Fragments arranged by size:
1618 798 611 490 213 191 190 176
93 79 74 62 24
BspHI T'CATG_A
Cuts at: 3051 3051
Size: 4619
BspMI ACCTGCnnnn'nnnn_
Cuts at: 370 1669 1883 2185 2566 370
Size: 1299 214 302 381 2423
Fragments arranged by size:
2423 1299 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2911 2965 4449 4614 50
Size: 2861 54 1484 165 55
Fragments arranged by size:
2861 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 934 2531 934
Size: 1597 3022
BsrFI r'CCGG_y
Cuts at: 2617 2798 2617
Size: 181 4438
BssSI C'ACGA_G
Cuts at: 2890 3598 2890
Size: 708 3911
BstAPI GCAn_nnn'nTGC
Cuts at: 1996 1996
Size: 4619
BstBI TT'CG_AA
Cuts at: 2980 2980
Size: 4619
BstXI CCAn_nnnn'nTGG
Cuts at: 1503 1503
Size: 4619
BstYI r'GATC_y
Cuts at: 1 1644 2111 2134 2469 2715 3021 3033 3119
Size: 1643 467 23 335 246 306 12 86
Cuts at: 3119 4171 1
Size: 1052 449
Fragments arranged by size:
1643 1052 467 449 335 306 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 703 703
Size: 4619
BtgI C'CryG_G
Cuts at: 107 107
Size: 4619
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2569 2750 2913 2569
Size: 181 163 4275
Fragments arranged by size:
4275 181 163
BtsI GCAGTG_nn'
Cuts at: 845 1493 1597 1862 845
Size: 648 104 265 3602
Fragments arranged by size:
3602 648 265 104
DraI TTT'AAA
Cuts at: 1618 1618
Size: 4619
DrdI GACnn_nn'nnGTC
Cuts at: 835 2325 3669 835
Size: 1490 1344 1785
Fragments arranged by size:
1785 1490 1344
EaeI y'GGCC_r
Cuts at: 580 2204 2378 2769 2796 4141 580
Size: 1624 174 391 27 1345 1058
Fragments arranged by size:
1624 1345 1058 391 174 27
EagI C'GGCC_G
Cuts at: 2204 2204
Size: 4619
EarI CTCTTCn'nnn_
Cuts at: 230 1182 2642 2852 230
Size: 952 1460 210 1997
Fragments arranged by size:
1997 1460 952 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2827 3569 3715 4429 2827
Size: 742 146 714 3017
Fragments arranged by size:
3017 742 714 146
EcoICRI GAG'CTC
Cuts at: 1315 1631 1315
Size: 316 4303
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 559 673 1297 1314 2444 2876 3229 3891 559
Size: 114 624 17 1130 432 353 662 1287
Fragments arranged by size:
1287 1130 662 624 432 353 114 17
EcoO109I rG'GnC_Cy
Cuts at: 188 1758 188
Size: 1570 3049
EcoRI G'AATT_C
Cuts at: 649 1623 649
Size: 974 3645
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 564 796 2638 564
Size: 232 1842 2545
Fragments arranged by size:
2545 1842 232
FspI TGC'GCA
Cuts at: 2400 2400
Size: 4619
HaeII r_GCGC'y
Cuts at: 118 1050 1463 2053 2301 3531 4302 118
Size: 932 413 590 248 1230 771 435
Fragments arranged by size:
1230 932 771 590 435 413 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 577 609 1128 1160 1270 1302 1357 1389 2461
Size: 32 519 32 110 32 55 32 1072
Cuts at: 2461 2491 2493 2523 577
Size: 30 2 30 2673
Fragments arranged by size:
2673 1072 519 110 55 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1191 1658 1191
Size: 467 4152
HindIII A'AGCT_T
Cuts at: 470 1356 470
Size: 886 3733
KasI G'GCGC_C
Cuts at: 114 2049 2297 114
Size: 1935 248 2436
Fragments arranged by size:
2436 1935 248
KpnI G_GTAC'C
Cuts at: 1285 1639 1285
Size: 354 4265
MluI A'CGCG_T
Cuts at: 4052 4052
Size: 4619
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3378 3562 3378
Size: 184 4435
MscI TGG'CCA
Cuts at: 582 2380 582
Size: 1798 2821
MslI CAynn'nnrTG
Cuts at: 2608 2735 2608
Size: 127 4492
MspA1I CmG'CkG
Cuts at: 210 1531 1730 2044 2404 3188 3433 210
Size: 1321 199 314 360 784 245 1396
Fragments arranged by size:
1396 1321 784 360 314 245 199
NaeI GCC'GGC
Cuts at: 2800 2800
Size: 4619
NarI GG'CG_CC
Cuts at: 115 2050 2298 115
Size: 1935 248 2436
Fragments arranged by size:
2436 1935 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4619
NgoMIV G'CCGG_C
Cuts at: 2798 2798
Size: 4619
NheI G'CTAG_C
Cuts at: 1213 1213
Size: 4619
NsiI A_TGCA'T
Cuts at: 996 996
Size: 4619
NspI r_CATG'y
Cuts at: 923 1672 2172 3775 923
Size: 749 500 1603 1767
Fragments arranged by size:
1767 1603 749 500
PciI A'CATG_T
Cuts at: 3771 3771
Size: 4619
PmeI GTTT'AAAC
Cuts at: 1618 1618
Size: 4619
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3065 3097 3065
Size: 32 4587
PpuMI rG'GwC_Cy
Cuts at: 188 188
Size: 4619
PstI C_TGCA'G
Cuts at: 1666 2351 1666
Size: 685 3934
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4619
PvuII CAG'CTG
Cuts at: 210 1531 2044 2404 210
Size: 1321 513 360 2425
Fragments arranged by size:
2425 1321 513 360
RsrII CG'GwC_CG
Cuts at: 2814 2814
Size: 4619
SacI G_AGCT'C
Cuts at: 1317 1633 1317
Size: 316 4303
SalI G'TCGA_C
Cuts at: 1656 1656
Size: 4619
SanDI GG'GwC_CC
Cuts at: 188 188
Size: 4619
SapI GCTCTTCn'nnn_
Cuts at: 2642 2852 2642
Size: 210 4409
SbfI CC_TGCA'GG
Cuts at: 1666 1666
Size: 4619
ScaI AGT'ACT
Cuts at: 4136 4136
Size: 4619
SfcI C'TryA_G
Cuts at: 33 1662 2347 3315 3506 33
Size: 1629 685 968 191 1146
Fragments arranged by size:
1629 1146 968 685 191
SfoI GGC'GCC
Cuts at: 116 2051 2299 116
Size: 1935 248 2436
Fragments arranged by size:
2436 1935 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4619
SmaI CCC'GGG
Cuts at: 1641 1641
Size: 4619
SmlI C'TyrA_G
Cuts at: 451 1030 1774 2689 3403 3665 451
Size: 579 744 915 714 262 1405
Fragments arranged by size:
1405 915 744 714 579 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4619
SphI G_CATG'C
Cuts at: 1672 2172 1672
Size: 500 4119
SspI AAT'ATT
Cuts at: 1583 1583
Size: 4619
StuI AGG'CCT
Cuts at: 1028 1028
Size: 4619
StyI C'CwwG_G
Cuts at: 107 1396 1753 2107 107
Size: 1289 357 354 2619
Fragments arranged by size:
2619 1289 357 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 617 1389 2202 2335 2794 3003 617
Size: 772 813 133 459 209 2233
Fragments arranged by size:
2233 813 772 459 209 133
TatI w'GTAC_w
Cuts at: 553 4134 553
Size: 3581 1038
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1588 1620 4136 4168 1588
Size: 32 2516 32 2039
Fragments arranged by size:
2516 2039 32 32
Tth111I GACn'n_nGTC
Cuts at: 2416 2416
Size: 4619
XbaI T'CTAG_A
Cuts at: 1650 1650
Size: 4619
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 283 984 2114 283
Size: 701 1130 2788
Fragments arranged by size:
2788 1130 701
XmaI C'CCGG_G
Cuts at: 1639 1639
Size: 4619
XmnI GAAnn'nnTTC
Cuts at: 1573 1573
Size: 4619
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NheI NsiI NspI PciI
PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AgeI AhdI AleI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BsaXI BsiWI BsmI
BsmBI BspEI BsrGI BssHII BstEII BstZ17I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HpaI MfeI NdeI
NotI NruI PacI PasI PflMI PfoI PmlI PshAI
PsiI PspOMI PspXI PsrI SacII SexAI SfiI SgrAI
SnaBI SrfI SwaI XhoI ZraI