Restriction map for
pF1KB8634
(Circular) (Six-base) MAPSORT of: pF1KB8634.seq Check: 6750 from: 1 to: 4136
pF1KB8634 4136 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AccI GT'mk_AC
Cuts at: 460 1174 460
Size: 714 3422
Acc65I G'GTAC_C
Cuts at: 1152 1152
Size: 4136
AclI AA'CG_TT
Cuts at: 3976 3976
Size: 4136
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 269 1961 2393 2746 3408 269
Size: 1692 432 353 662 997
Fragments arranged by size:
1692 997 662 432 353
AfeI AGC'GCT
Cuts at: 1075 1075
Size: 4136
AflIII A'CryG_T
Cuts at: 1488 3288 3569 1488
Size: 1800 281 2055
Fragments arranged by size:
2055 1800 281
AgeI A'CCGG_T
Cuts at: 623 623
Size: 4136
AleI CACnn'nnGTG
Cuts at: 685 685
Size: 4136
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 387 419 387
Size: 32 4104
AlwNI CAG_nnn'CTG
Cuts at: 407 828 1441 2879 407
Size: 421 613 1438 1664
Fragments arranged by size:
1664 1438 613 421
ApaLI G'TGCA_C
Cuts at: 2974 2974
Size: 4136
ApoI r'AATT_y
Cuts at: 274 1140 3489 3745 274
Size: 866 2349 256 665
Fragments arranged by size:
2349 866 665 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4136
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4136
AvaI C'yCGr_G
Cuts at: 296 1156 296
Size: 860 3276
BamHI G'GATC_C
Cuts at: 1161 1161
Size: 4136
BanI G'GyrC_C
Cuts at: 1152 1566 1814 1849 1152
Size: 414 248 35 3439
Fragments arranged by size:
3439 414 248 35
BanII G_rGCy'C
Cuts at: 986 1005 1150 2180 986
Size: 19 145 1030 2942
Fragments arranged by size:
2942 1030 145 19
BbeI G_GCGC'C
Cuts at: 1570 1818 1570
Size: 248 3888
BciVI GTATCCnnnnn_n'
Cuts at: 2034 3090 4136 2034
Size: 1056 1046 2034
Fragments arranged by size:
2034 1056 1046
BclI T'GATC_A
Cuts at: 1656 1656
Size: 4136
BglII A'GATC_T
Cuts at: 1651 3688 1651
Size: 2037 2099
BlpI GC'TnA_GC
Cuts at: 653 1077 1248 653
Size: 424 171 3541
Fragments arranged by size:
3541 424 171
Bme1580I G_kGCm'C
Cuts at: 1761 1854 2978 1761
Size: 93 1124 2919
Fragments arranged by size:
2919 1124 93
BmgBI CAC'GTC
Cuts at: 336 336
Size: 4136
BmrI ACTGGGnnnn_n'
Cuts at: 1348 1429 1764 3908 1348
Size: 81 335 2144 1576
Fragments arranged by size:
2144 1576 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1038 2503 1038
Size: 1465 2671
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 196 362 196
Size: 166 3970
Bpu10I CC'TnA_GC
Cuts at: 202 967 988 202
Size: 765 21 3350
Fragments arranged by size:
3350 765 21
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 439 1033 1312 2191 2905 3203 439
Size: 594 279 879 714 298 1372
Fragments arranged by size:
1372 879 714 594 298 279
BsaI GGTCTCn'nnnn_
Cuts at: 3840 3840
Size: 4136
BsaAI yAC'GTr
Cuts at: 1489 1489
Size: 4136
BsaBI GATnn'nnATC
Cuts at: 6 548 724 1650 3687 6
Size: 542 176 926 2037 455
Fragments arranged by size:
2037 926 542 455 176
BsaHI Gr'CG_yC
Cuts at: 1567 1815 2517 4013 1567
Size: 248 702 1496 1690
Fragments arranged by size:
1690 1496 702 248
BsaWI w'CCGG_w
Cuts at: 152 623 1325 1846 2524 2935 3082 3356 152
Size: 471 702 521 678 411 147 274 932
Fragments arranged by size:
932 702 678 521 471 411 274 147
BseYI C'CCAG_C
Cuts at: 1116 1561 2319 2984 3392 1116
Size: 445 758 665 408 1860
Fragments arranged by size:
1860 758 665 445 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1106 1106
Size: 4136
BsiEI CG_ry'CG
Cuts at: 104 246 1678 1724 2954 104
Size: 142 1432 46 1230 1286
Fragments arranged by size:
1432 1286 1230 142 46
BsiHKAI G_wGCw'C
Cuts at: 1150 1928 2118 2978 1150
Size: 778 190 860 2308
Fragments arranged by size:
2308 860 778 190
BsmI GAATG_Cn'
Cuts at: 165 165
Size: 4136
Bsp1286I G_dGCh'C
Cuts at: 986 1005 1150 1761 1854 1928 2118 2180 2978
Size: 19 145 611 93 74 190 62 798
Cuts at: 2978 986
Size: 2144
Fragments arranged by size:
2144 798 611 190 145 93 74 62
19
BspEI T'CCGG_A
Cuts at: 152 152
Size: 4136
BspHI T'CATG_A
Cuts at: 2568 2568
Size: 4136
BspMI ACCTGCnnnn'nnnn_
Cuts at: 236 1186 1400 1702 2083 236
Size: 950 214 302 381 2289
Fragments arranged by size:
2289 950 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2428 2482 3966 4131 50
Size: 2378 54 1484 165 55
Fragments arranged by size:
2378 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2048 2048
Size: 4136
BsrFI r'CCGG_y
Cuts at: 185 623 2134 2315 185
Size: 438 1511 181 2006
Fragments arranged by size:
2006 1511 438 181
BssSI C'ACGA_G
Cuts at: 123 2407 3115 123
Size: 2284 708 1144
Fragments arranged by size:
2284 1144 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1513 1513
Size: 4136
BstBI TT'CG_AA
Cuts at: 565 2497 565
Size: 1932 2204
BstYI r'GATC_y
Cuts at: 1 824 1060 1161 1628 1651 1986 2232 2538
Size: 823 236 101 467 23 335 246 306
Cuts at: 2538 2550 2636 3688 1
Size: 12 86 1052 449
Fragments arranged by size:
1052 823 467 449 335 306 246 236
101 86 23 12
BstZ17I GTA'TAC
Cuts at: 461 461
Size: 4136
BtgI C'CryG_G
Cuts at: 107 107
Size: 4136
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2086 2267 2430 2086
Size: 181 163 3792
Fragments arranged by size:
3792 181 163
BtsI GCAGTG_nn'
Cuts at: 1379 1379
Size: 4136
DraI TTT'AAA
Cuts at: 1135 1135
Size: 4136
DrdI GACnn_nn'nnGTC
Cuts at: 1842 3186 1842
Size: 1344 2792
EaeI y'GGCC_r
Cuts at: 187 734 1721 1895 2286 2313 3658 187
Size: 547 987 174 391 27 1345 665
Fragments arranged by size:
1345 987 665 547 391 174 27
EagI C'GGCC_G
Cuts at: 1721 1721
Size: 4136
EarI CTCTTCn'nnn_
Cuts at: 364 2159 2369 364
Size: 1795 210 2131
Fragments arranged by size:
2131 1795 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2344 3086 3232 3946 2344
Size: 742 146 714 2534
Fragments arranged by size:
2534 742 714 146
EcoICRI GAG'CTC
Cuts at: 1148 1148
Size: 4136
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 196 269 362 1961 2393 2746 3408 196
Size: 73 93 1599 432 353 662 924
Fragments arranged by size:
1599 924 662 432 353 93 73
EcoNI CCTnn'n_nnAGG
Cuts at: 1033 1033
Size: 4136
EcoO109I rG'GnC_Cy
Cuts at: 510 1050 1275 510
Size: 540 225 3371
Fragments arranged by size:
3371 540 225
EcoRI G'AATT_C
Cuts at: 274 1140 274
Size: 866 3270
EcoRV GAT'ATC
Cuts at: 726 726
Size: 4136
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 375 2155 375
Size: 1780 2356
FspI TGC'GCA
Cuts at: 1917 1917
Size: 4136
HaeII r_GCGC'y
Cuts at: 1077 1570 1818 3048 3819 1077
Size: 493 248 1230 771 1394
Fragments arranged by size:
1394 1230 771 493 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1978 2008 2010 2040 1978
Size: 30 2 30 4074
Fragments arranged by size:
4074 30 30 2
HincII GTy'rAC
Cuts at: 445 1175 445
Size: 730 3406
HindIII A'AGCT_T
Cuts at: 1009 1009
Size: 4136
KasI G'GCGC_C
Cuts at: 1566 1814 1566
Size: 248 3888
KpnI G_GTAC'C
Cuts at: 1156 1156
Size: 4136
MluI A'CGCG_T
Cuts at: 3569 3569
Size: 4136
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2895 3079 2895
Size: 184 3952
MscI TGG'CCA
Cuts at: 1897 1897
Size: 4136
MslI CAynn'nnrTG
Cuts at: 685 841 2125 2252 685
Size: 156 1284 127 2569
Fragments arranged by size:
2569 1284 156 127
MspA1I CmG'CkG
Cuts at: 657 1247 1561 1921 2705 2950 657
Size: 590 314 360 784 245 1843
Fragments arranged by size:
1843 784 590 360 314 245
NaeI GCC'GGC
Cuts at: 2317 2317
Size: 4136
NarI GG'CG_CC
Cuts at: 1567 1815 1567
Size: 248 3888
NcoI C'CATG_G
Cuts at: 107 107
Size: 4136
NgoMIV G'CCGG_C
Cuts at: 2315 2315
Size: 4136
NspI r_CATG'y
Cuts at: 1189 1689 3292 1189
Size: 500 1603 2033
Fragments arranged by size:
2033 1603 500
PciI A'CATG_T
Cuts at: 3288 3288
Size: 4136
PflMI CCAn_nnn'nTGG
Cuts at: 785 785
Size: 4136
PfoI T'CCnGG_A
Cuts at: 378 378
Size: 4136
PmeI GTTT'AAAC
Cuts at: 1135 1135
Size: 4136
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 106 138 2582 2614 106
Size: 32 2444 32 1628
Fragments arranged by size:
2444 1628 32 32
PpuMI rG'GwC_Cy
Cuts at: 510 1050 510
Size: 540 3596
PstI C_TGCA'G
Cuts at: 233 890 1183 1868 233
Size: 657 293 685 2501
Fragments arranged by size:
2501 685 657 293
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4136
PvuII CAG'CTG
Cuts at: 657 1561 1921 657
Size: 904 360 2872
Fragments arranged by size:
2872 904 360
RsrII CG'GwC_CG
Cuts at: 2331 2331
Size: 4136
SacI G_AGCT'C
Cuts at: 1150 1150
Size: 4136
SalI G'TCGA_C
Cuts at: 1173 1173
Size: 4136
SanDI GG'GwC_CC
Cuts at: 510 510
Size: 4136
SapI GCTCTTCn'nnn_
Cuts at: 2159 2369 2159
Size: 210 3926
SbfI CC_TGCA'GG
Cuts at: 1183 1183
Size: 4136
ScaI AGT'ACT
Cuts at: 3653 3653
Size: 4136
SfcI C'TryA_G
Cuts at: 33 229 886 1179 1864 2832 3023 33
Size: 196 657 293 685 968 191 1146
Fragments arranged by size:
1146 968 685 657 293 196 191
SfoI GGC'GCC
Cuts at: 1568 1816 1568
Size: 248 3888
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4136
SmaI CCC'GGG
Cuts at: 1158 1158
Size: 4136
SmlI C'TyrA_G
Cuts at: 454 1012 1291 2206 2920 3182 454
Size: 558 279 915 714 262 1408
Fragments arranged by size:
1408 915 714 558 279 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4136
SphI G_CATG'C
Cuts at: 1189 1689 1189
Size: 500 3636
SspI AAT'ATT
Cuts at: 311 311
Size: 4136
StyI C'CwwG_G
Cuts at: 107 779 977 1270 1624 107
Size: 672 198 293 354 2619
Fragments arranged by size:
2619 672 354 293 198
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1719 1852 2311 2520 1719
Size: 133 459 209 3335
Fragments arranged by size:
3335 459 209 133
TatI w'GTAC_w
Cuts at: 1043 3651 1043
Size: 2608 1528
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3653 3685 3653
Size: 32 4104
Tth111I GACn'n_nGTC
Cuts at: 1933 1933
Size: 4136
XbaI T'CTAG_A
Cuts at: 1167 1167
Size: 4136
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 681 1631 681
Size: 950 3186
XmaI C'CCGG_G
Cuts at: 1156 1156
Size: 4136
XmnI GAAnn'nnTTC
Cuts at: 551 551
Size: 4136
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI BstZ17I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PflMI PfoI
PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AlfI ApaI AscI AvrII
BaeI BbsI BbvCI BcgI BfrBI BglI BmtI BsaXI
BseRI BsiWI BsmBI BsrGI BssHII BstEII BstXI Bsu36I
ClaI CspCI DraIII FseI FspAI HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PmlI PshAI
PsiI PspOMI PspXI PsrI SacII SexAI SfiI SgrAI
SnaBI SrfI StuI SwaI XhoI ZraI