Restriction map for
pF1KB8635
(Circular) (Six-base) MAPSORT of: pF1KB8635.seq Check: 3857 from: 1 to: 3836
pF1KB8635 3836 bp
With 182 enzymes: SgfI *
March 2, 2010 14:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 330 330
Size: 3836
AccI GT'mk_AC
Cuts at: 874 874
Size: 3836
Acc65I G'GTAC_C
Cuts at: 852 852
Size: 3836
AclI AA'CG_TT
Cuts at: 3676 3676
Size: 3836
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1661 2093 2840 3108 1661
Size: 432 747 268 2389
Fragments arranged by size:
2389 747 432 268
AflIII A'CryG_T
Cuts at: 212 1188 2292 3269 212
Size: 976 1104 977 779
Fragments arranged by size:
1104 977 976 779
AgeI A'CCGG_T
Cuts at: 2224 2224
Size: 3836
AleI CACnn'nnGTG
Cuts at: 528 528
Size: 3836
AlwNI CAG_nnn'CTG
Cuts at: 194 1141 2708 194
Size: 947 1567 1322
Fragments arranged by size:
1567 1322 947
ApaI G_GGCC'C
Cuts at: 295 295
Size: 3836
ApaLI G'TGCA_C
Cuts at: 2606 2606
Size: 3836
ApoI r'AATT_y
Cuts at: 840 3189 3445 840
Size: 2349 256 1231
Fragments arranged by size:
2349 1231 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3836
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3836
AvaI C'yCGr_G
Cuts at: 492 570 856 492
Size: 78 286 3472
Fragments arranged by size:
3472 286 78
BamHI G'GATC_C
Cuts at: 861 861
Size: 3836
BanI G'GyrC_C
Cuts at: 477 852 1266 1514 1549 477
Size: 375 414 248 35 2764
Fragments arranged by size:
2764 414 375 248 35
BanII G_rGCy'C
Cuts at: 295 690 850 1880 295
Size: 395 160 1030 2251
Fragments arranged by size:
2251 1030 395 160
BbeI G_GCGC'C
Cuts at: 481 1270 1518 481
Size: 789 248 2799
Fragments arranged by size:
2799 789 248
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 473 507 90
Size: 34 349 34 3419
Fragments arranged by size:
3419 349 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1734 2495 3836 1734
Size: 761 1341 1734
Fragments arranged by size:
1734 1341 761
BclI T'GATC_A
Cuts at: 1356 1356
Size: 3836
BglII A'GATC_T
Cuts at: 1351 3388 1351
Size: 2037 1799
BlpI GC'TnA_GC
Cuts at: 948 948
Size: 3836
Bme1580I G_kGCm'C
Cuts at: 295 1461 1554 2610 295
Size: 1166 93 1056 1521
Fragments arranged by size:
1521 1166 1056 93
BmgBI CAC'GTC
Cuts at: 213 213
Size: 3836
BmrI ACTGGGnnnn_n'
Cuts at: 1048 1129 1464 3608 1048
Size: 81 335 2144 1276
Fragments arranged by size:
2144 1276 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2203 2203
Size: 3836
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 253 312 665 253
Size: 59 353 3424
Fragments arranged by size:
3424 353 59
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 349 1012 1891 2383 2681 349
Size: 663 879 492 298 1504
Fragments arranged by size:
1504 879 663 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 634 3540 634
Size: 2906 930
BsaAI yAC'GTr
Cuts at: 1189 1189
Size: 3836
BsaBI GATnn'nnATC
Cuts at: 6 1350 3387 6
Size: 1344 2037 455
Fragments arranged by size:
2037 1344 455
BsaHI Gr'CG_yC
Cuts at: 478 1267 1515 2217 3713 478
Size: 789 248 702 1496 601
Fragments arranged by size:
1496 789 702 601 248
BsaWI w'CCGG_w
Cuts at: 1025 1546 2224 2498 2645 3056 1025
Size: 521 678 274 147 411 1805
Fragments arranged by size:
1805 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 136 166 677 707 136
Size: 30 511 30 3265
Fragments arranged by size:
3265 511 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 415 730 752 415
Size: 315 22 3499
Fragments arranged by size:
3499 315 22
BseYI C'CCAG_C
Cuts at: 119 200 634 1261 2019 2596 3092 119
Size: 81 434 627 758 577 496 863
Fragments arranged by size:
863 758 627 577 496 434 81
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 127 229 358 366 467 127
Size: 102 129 8 101 3496
Fragments arranged by size:
3496 129 102 101 8
BsiEI CG_ry'CG
Cuts at: 104 506 1378 1424 2632 104
Size: 402 872 46 1208 1308
Fragments arranged by size:
1308 1208 872 402 46
BsiHKAI G_wGCw'C
Cuts at: 850 1628 1818 2610 850
Size: 778 190 792 2076
Fragments arranged by size:
2076 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 295 690 850 1461 1554 1628 1818 1880 2610
Size: 395 160 611 93 74 190 62 730
Cuts at: 2610 295
Size: 1521
Fragments arranged by size:
1521 730 611 395 190 160 93 74
62
BspEI T'CCGG_A
Cuts at: 3056 3056
Size: 3836
BspHI T'CATG_A
Cuts at: 3012 3012
Size: 3836
BspMI ACCTGCnnnn'nnnn_
Cuts at: 202 330 819 886 1100 1402 1783 202
Size: 128 489 67 214 302 381 2255
Fragments arranged by size:
2255 489 381 302 214 128 67
BsrBI CCG'CTC
Cuts at: 50 760 2128 2182 3666 3831 50
Size: 710 1368 54 1484 165 55
Fragments arranged by size:
1484 1368 710 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1748 1748
Size: 3836
BsrFI r'CCGG_y
Cuts at: 395 648 1834 2015 2224 395
Size: 253 1186 181 209 2007
Fragments arranged by size:
2007 1186 253 209 181
BssHII G'CGCG_C
Cuts at: 598 598
Size: 3836
BssSI C'ACGA_G
Cuts at: 400 2107 2465 400
Size: 1707 358 1771
Fragments arranged by size:
1771 1707 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1213 1213
Size: 3836
BstBI TT'CG_AA
Cuts at: 2197 2197
Size: 3836
BstYI r'GATC_y
Cuts at: 1 861 1328 1351 1686 1932 2944 3030 3042
Size: 860 467 23 335 246 1012 86 12
Cuts at: 3042 3388 1
Size: 346 449
Fragments arranged by size:
1012 860 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 463 463
Size: 3836
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1786 1967 2130 1786
Size: 181 163 3492
Fragments arranged by size:
3492 181 163
BtsI GCAGTG_nn'
Cuts at: 1079 1079
Size: 3836
DraI TTT'AAA
Cuts at: 835 835
Size: 3836
DraIII CAC_nnn'GTG
Cuts at: 213 213
Size: 3836
DrdI GACnn_nn'nnGTC
Cuts at: 1542 2400 1542
Size: 858 2978
EaeI y'GGCC_r
Cuts at: 1421 1595 1986 2013 3358 1421
Size: 174 391 27 1345 1899
Fragments arranged by size:
1899 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1421 1421
Size: 3836
EarI CTCTTCn'nnn_
Cuts at: 154 172 1859 2069 154
Size: 18 1687 210 1921
Fragments arranged by size:
1921 1687 210 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 640 829 2044 2354 2500 3646 640
Size: 189 1215 310 146 1146 830
Fragments arranged by size:
1215 1146 830 310 189 146
EcoICRI GAG'CTC
Cuts at: 848 848
Size: 3836
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 253 312 665 1661 2093 2840 3108 253
Size: 59 353 996 432 747 268 981
Fragments arranged by size:
996 981 747 432 353 268 59
EcoNI CCTnn'n_nnAGG
Cuts at: 163 163
Size: 3836
EcoO109I rG'GnC_Cy
Cuts at: 191 222 538 975 191
Size: 31 316 437 3052
Fragments arranged by size:
3052 437 316 31
EcoRI G'AATT_C
Cuts at: 840 840
Size: 3836
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 297 390 1855 297
Size: 93 1465 2278
Fragments arranged by size:
2278 1465 93
FspI TGC'GCA
Cuts at: 1617 1617
Size: 3836
HaeII r_GCGC'y
Cuts at: 481 1270 1518 2540 3519 481
Size: 789 248 1022 979 798
Fragments arranged by size:
1022 979 798 789 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 553 585 1678 1708 1710 1740 553
Size: 32 1093 30 2 30 2649
Fragments arranged by size:
2649 1093 32 30 30 2
HincII GTy'rAC
Cuts at: 875 875
Size: 3836
KasI G'GCGC_C
Cuts at: 477 1266 1514 477
Size: 789 248 2799
Fragments arranged by size:
2799 789 248
KpnI G_GTAC'C
Cuts at: 856 856
Size: 3836
MluI A'CGCG_T
Cuts at: 3269 3269
Size: 3836
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 492 2507 2691 492
Size: 2015 184 1637
Fragments arranged by size:
2015 1637 184
MscI TGG'CCA
Cuts at: 1597 1597
Size: 3836
MslI CAynn'nnrTG
Cuts at: 528 1825 1952 528
Size: 1297 127 2412
Fragments arranged by size:
2412 1297 127
MspA1I CmG'CkG
Cuts at: 947 1261 1621 2634 2879 947
Size: 314 360 1013 245 1904
Fragments arranged by size:
1904 1013 360 314 245
NaeI GCC'GGC
Cuts at: 397 650 2017 397
Size: 253 1367 2216
Fragments arranged by size:
2216 1367 253
NarI GG'CG_CC
Cuts at: 478 1267 1515 478
Size: 789 248 2799
Fragments arranged by size:
2799 789 248
NgoMIV G'CCGG_C
Cuts at: 395 648 2015 395
Size: 253 1367 2216
Fragments arranged by size:
2216 1367 253
NspI r_CATG'y
Cuts at: 889 1389 2296 889
Size: 500 907 2429
Fragments arranged by size:
2429 907 500
PciI A'CATG_T
Cuts at: 2292 2292
Size: 3836
PflMI CCAn_nnn'nTGG
Cuts at: 320 320
Size: 3836
PfoI T'CCnGG_A
Cuts at: 654 681 654
Size: 27 3809
PmeI GTTT'AAAC
Cuts at: 835 835
Size: 3836
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2975 3007 2975
Size: 32 3804
PpuMI rG'GwC_Cy
Cuts at: 191 222 538 191
Size: 31 316 3489
Fragments arranged by size:
3489 316 31
PspOMI G'GGCC_C
Cuts at: 291 291
Size: 3836
PstI C_TGCA'G
Cuts at: 394 883 1568 394
Size: 489 685 2662
Fragments arranged by size:
2662 685 489
PvuI CG_AT'CG
Cuts at: 104 506 104
Size: 402 3434
PvuII CAG'CTG
Cuts at: 1261 1621 1261
Size: 360 3476
RsrII CG'GwC_CG
Cuts at: 2031 2031
Size: 3836
SacI G_AGCT'C
Cuts at: 850 850
Size: 3836
SalI G'TCGA_C
Cuts at: 873 873
Size: 3836
SapI GCTCTTCn'nnn_
Cuts at: 172 1859 2069 172
Size: 1687 210 1939
Fragments arranged by size:
1939 1687 210
SbfI CC_TGCA'GG
Cuts at: 883 883
Size: 3836
ScaI AGT'ACT
Cuts at: 3353 3353
Size: 3836
SfcI C'TryA_G
Cuts at: 33 390 879 1564 2557 2748 33
Size: 357 489 685 993 191 1121
Fragments arranged by size:
1121 993 685 489 357 191
SfoI GGC'GCC
Cuts at: 479 1268 1516 479
Size: 789 248 2799
Fragments arranged by size:
2799 789 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3836
SmaI CCC'GGG
Cuts at: 858 858
Size: 3836
SmlI C'TyrA_G
Cuts at: 364 991 1906 2398 2660 364
Size: 627 915 492 262 1540
Fragments arranged by size:
1540 915 627 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3836
SphI G_CATG'C
Cuts at: 889 1389 889
Size: 500 3336
StyI C'CwwG_G
Cuts at: 970 1324 970
Size: 354 3482
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 531 1419 1552 2011 2220 531
Size: 888 133 459 209 2147
Fragments arranged by size:
2147 888 459 209 133
TatI w'GTAC_w
Cuts at: 3351 3351
Size: 3836
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 136 168 238 270 677 709 3353 3385 136
Size: 32 70 32 407 32 2644 32 587
Fragments arranged by size:
2644 587 407 70 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1633 1633
Size: 3836
XbaI T'CTAG_A
Cuts at: 867 867
Size: 3836
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1331 1331
Size: 3836
XmaI C'CCGG_G
Cuts at: 856 856
Size: 3836
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BcgI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PciI PflMI PfoI PmeI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI AscI AvrII
BaeI BbsI BbvCI BfrBI BglI BmtI Bpu10I BsiWI
BsmI BsmBI BsrGI BstEII BstXI BstZ17I Bsu36I ClaI
CspCI EcoRV FseI FspAI HindIII HpaI MfeI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PmlI
PshAI PsiI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI