Restriction map for
pF1KB8641
(Circular) (Six-base) MAPSORT of: pF1KB8641.seq Check: 4065 from: 1 to: 5690
pF1KB8641 5690 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 789 2498 789
Size: 1709 3981
AccI GT'mk_AC
Cuts at: 2728 2728
Size: 5690
Acc65I G'GTAC_C
Cuts at: 2706 2706
Size: 5690
AclI AA'CG_TT
Cuts at: 5530 5530
Size: 5690
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 455 674 1001 2491 3515 3947 4300 4962 455
Size: 219 327 1490 1024 432 353 662 1183
Fragments arranged by size:
1490 1183 1024 662 432 353 327 219
AflIII A'CryG_T
Cuts at: 2405 3042 4842 5123 2405
Size: 637 1800 281 2972
Fragments arranged by size:
2972 1800 637 281
AleI CACnn'nnGTG
Cuts at: 1642 2129 1642
Size: 487 5203
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1346 1346
Size: 5690
AlwNI CAG_nnn'CTG
Cuts at: 2377 2505 2995 4433 2377
Size: 128 490 1438 3634
Fragments arranged by size:
3634 1438 490 128
ApaLI G'TGCA_C
Cuts at: 4528 4528
Size: 5690
ApoI r'AATT_y
Cuts at: 157 457 2694 5043 5299 157
Size: 300 2237 2349 256 548
Fragments arranged by size:
2349 2237 548 300 256
AseI AT'TA_AT
Cuts at: 20 1459 20
Size: 1439 4251
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5690
AvaI C'yCGr_G
Cuts at: 1295 2051 2144 2710 1295
Size: 756 93 566 4275
Fragments arranged by size:
4275 756 566 93
BamHI G'GATC_C
Cuts at: 2715 2715
Size: 5690
BanI G'GyrC_C
Cuts at: 624 777 1505 2706 3120 3368 3403 624
Size: 153 728 1201 414 248 35 2911
Fragments arranged by size:
2911 1201 728 414 248 153 35
BanII G_rGCy'C
Cuts at: 1562 1925 2704 3734 1562
Size: 363 779 1030 3518
Fragments arranged by size:
3518 1030 779 363
BbeI G_GCGC'C
Cuts at: 3124 3372 3124
Size: 248 5442
BbsI GAAGACnn'nnnn_
Cuts at: 646 2024 2408 2632 646
Size: 1378 384 224 3704
Fragments arranged by size:
3704 1378 384 224
BciVI GTATCCnnnnn_n'
Cuts at: 1874 2543 3588 4644 5690 1874
Size: 669 1045 1056 1046 1874
Fragments arranged by size:
1874 1056 1046 1045 669
BclI T'GATC_A
Cuts at: 663 1139 2195 3210 663
Size: 476 1056 1015 3143
Fragments arranged by size:
3143 1056 1015 476
BfrBI ATG'CAT
Cuts at: 997 997
Size: 5690
BglII A'GATC_T
Cuts at: 3205 5242 3205
Size: 2037 3653
BlpI GC'TnA_GC
Cuts at: 1556 2802 1556
Size: 1246 4444
Bme1580I G_kGCm'C
Cuts at: 629 3315 3408 4532 629
Size: 2686 93 1124 1787
Fragments arranged by size:
2686 1787 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1031 2902 2983 3318 5462 1031
Size: 1871 81 335 2144 1259
Fragments arranged by size:
2144 1871 1259 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4057 4057
Size: 5690
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 288 502 950 1091 1424 2017 2404 288
Size: 214 448 141 333 593 387 3574
Fragments arranged by size:
3574 593 448 387 333 214 141
Bpu10I CC'TnA_GC
Cuts at: 476 1501 476
Size: 1025 4665
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2209 2866 3745 4459 4757 2209
Size: 657 879 714 298 3142
Fragments arranged by size:
3142 879 714 657 298
BsaI GGTCTCn'nnnn_
Cuts at: 288 928 1795 5394 288
Size: 640 867 3599 584
Fragments arranged by size:
3599 867 640 584
BsaAI yAC'GTr
Cuts at: 2104 3043 2104
Size: 939 4751
BsaBI GATnn'nnATC
Cuts at: 6 3204 5241 6
Size: 3198 2037 455
Fragments arranged by size:
3198 2037 455
BsaHI Gr'CG_yC
Cuts at: 3121 3369 4071 5567 3121
Size: 248 702 1496 3244
Fragments arranged by size:
3244 1496 702 248
BsaWI w'CCGG_w
Cuts at: 516 1529 2879 3400 4078 4489 4636 4910 516
Size: 1013 1350 521 678 411 147 274 1296
Fragments arranged by size:
1350 1296 1013 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 364 394 1064 1094 1700 1730 1882 1912 364
Size: 30 670 30 606 30 152 30 4142
Fragments arranged by size:
4142 670 606 152 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 376 379 382 385 893 1192 1915 376
Size: 3 3 3 508 299 723 4151
Fragments arranged by size:
4151 723 508 299 3 3 3
BseYI C'CCAG_C
Cuts at: 523 2498 3115 3873 4538 4946 523
Size: 1975 617 758 665 408 1267
Fragments arranged by size:
1975 1267 758 665 617 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 731 2278 2332 731
Size: 1547 54 4089
Fragments arranged by size:
4089 1547 54
BsiEI CG_ry'CG
Cuts at: 104 3232 3278 4508 104
Size: 3128 46 1230 1286
Fragments arranged by size:
3128 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 613 726 1562 2704 3482 3672 4532 613
Size: 113 836 1142 778 190 860 1771
Fragments arranged by size:
1771 1142 860 836 778 190 113
BsmI GAATG_Cn'
Cuts at: 897 897
Size: 5690
BsmBI CGTCTCn'nnnn_
Cuts at: 1783 1783
Size: 5690
Bsp1286I G_dGCh'C
Cuts at: 613 629 726 1562 1925 2704 3315 3408 3482
Size: 16 97 836 363 779 611 93 74
Cuts at: 3482 3672 3734 4532 613
Size: 190 62 798 1771
Fragments arranged by size:
1771 836 798 779 611 363 190 97
93 74 62 16
BspEI T'CCGG_A
Cuts at: 516 516
Size: 5690
BspHI T'CATG_A
Cuts at: 660 4122 660
Size: 3462 2228
BspMI ACCTGCnnnn'nnnn_
Cuts at: 789 2498 2740 2954 3256 3637 789
Size: 1709 242 214 302 381 2842
Fragments arranged by size:
2842 1709 381 302 242 214
BsrBI CCG'CTC
Cuts at: 50 3982 4036 5520 5685 50
Size: 3932 54 1484 165 55
Fragments arranged by size:
3932 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 997 3602 997
Size: 2605 3085
BsrFI r'CCGG_y
Cuts at: 711 1445 3688 3869 711
Size: 734 2243 181 2532
Fragments arranged by size:
2532 2243 734 181
BssSI C'ACGA_G
Cuts at: 3961 4669 3961
Size: 708 4982
BstAPI GCAn_nnn'nTGC
Cuts at: 1404 2377 3067 1404
Size: 973 690 4027
Fragments arranged by size:
4027 973 690
BstBI TT'CG_AA
Cuts at: 4051 4051
Size: 5690
BstXI CCAn_nnnn'nTGG
Cuts at: 1692 2131 1692
Size: 439 5251
BstYI r'GATC_y
Cuts at: 1 471 1808 2715 3182 3205 3540 3786 4092
Size: 470 1337 907 467 23 335 246 306
Cuts at: 4092 4104 4190 5242 1
Size: 12 86 1052 449
Fragments arranged by size:
1337 1052 907 470 467 449 335 306
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2186 2186
Size: 5690
BtgI C'CryG_G
Cuts at: 1781 1781
Size: 5690
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3640 3821 3984 3640
Size: 181 163 5346
Fragments arranged by size:
5346 181 163
BtsI GCAGTG_nn'
Cuts at: 611 883 1030 2933 611
Size: 272 147 1903 3368
Fragments arranged by size:
3368 1903 272 147
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2024 2059 2024
Size: 35 5655
DraI TTT'AAA
Cuts at: 2689 2689
Size: 5690
DrdI GACnn_nn'nnGTC
Cuts at: 3396 4740 3396
Size: 1344 4346
EaeI y'GGCC_r
Cuts at: 1304 1337 3275 3449 3840 3867 5212 1304
Size: 33 1938 174 391 27 1345 1782
Fragments arranged by size:
1938 1782 1345 391 174 33 27
EagI C'GGCC_G
Cuts at: 3275 3275
Size: 5690
EarI CTCTTCn'nnn_
Cuts at: 316 353 880 3713 3923 316
Size: 37 527 2833 210 2083
Fragments arranged by size:
2833 2083 527 210 37
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3898 4640 4786 5500 3898
Size: 742 146 714 4088
Fragments arranged by size:
4088 742 714 146
EcoICRI GAG'CTC
Cuts at: 1560 2702 1560
Size: 1142 4548
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 288 455 502 674 950 1001 1091 1424 2017
Size: 167 47 172 276 51 90 333 593
Cuts at: 2017 2404 2491 3515 3947 4300 4962 288
Size: 387 87 1024 432 353 662 1016
Fragments arranged by size:
1024 1016 662 593 432 387 353 333
276 172 167 90 87 51 47
EcoO109I rG'GnC_Cy
Cuts at: 693 1126 2097 2829 693
Size: 433 971 732 3554
Fragments arranged by size:
3554 971 732 433
EcoRI G'AATT_C
Cuts at: 2694 2694
Size: 5690
EcoRV GAT'ATC
Cuts at: 1941 2241 1941
Size: 300 5390
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3709 3709
Size: 5690
FspI TGC'GCA
Cuts at: 3471 3471
Size: 5690
HaeII r_GCGC'y
Cuts at: 1241 3124 3372 4602 5373 1241
Size: 1883 248 1230 771 1558
Fragments arranged by size:
1883 1558 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 112 144 364 396 520 552 949 981 1064
Size: 32 220 32 124 32 397 32 83
Cuts at: 1064 1096 1194 1226 1278 1310 1848 1880 3532
Size: 32 98 32 52 32 538 32 1652
Cuts at: 3532 3562 3564 3594 112
Size: 30 2 30 2208
Fragments arranged by size:
2208 1652 538 397 220 124 98 83
52 32 32 32 32 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 2729 2729
Size: 5690
KasI G'GCGC_C
Cuts at: 3120 3368 3120
Size: 248 5442
KpnI G_GTAC'C
Cuts at: 2710 2710
Size: 5690
MluI A'CGCG_T
Cuts at: 5123 5123
Size: 5690
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 575 1205 2425 4449 4633 575
Size: 630 1220 2024 184 1632
Fragments arranged by size:
2024 1632 1220 630 184
MscI TGG'CCA
Cuts at: 1339 3451 1339
Size: 2112 3578
MslI CAynn'nnrTG
Cuts at: 856 1328 1642 2129 2275 3679 3806 856
Size: 472 314 487 146 1404 127 2740
Fragments arranged by size:
2740 1404 487 472 314 146 127
MspA1I CmG'CkG
Cuts at: 1569 2377 2801 3115 3475 4259 4504 1569
Size: 808 424 314 360 784 245 2755
Fragments arranged by size:
2755 808 784 424 360 314 245
NaeI GCC'GGC
Cuts at: 1447 3871 1447
Size: 2424 3266
NarI GG'CG_CC
Cuts at: 3121 3369 3121
Size: 248 5442
NgoMIV G'CCGG_C
Cuts at: 1445 3869 1445
Size: 2424 3266
NsiI A_TGCA'T
Cuts at: 999 999
Size: 5690
NspI r_CATG'y
Cuts at: 1963 2083 2409 2743 3243 4846 1963
Size: 120 326 334 500 1603 2807
Fragments arranged by size:
2807 1603 500 334 326 120
PacI TTA_AT'TAA
Cuts at: 1463 1463
Size: 5690
PasI CC'CwG_GG
Cuts at: 489 489
Size: 5690
PciI A'CATG_T
Cuts at: 2405 4842 2405
Size: 2437 3253
PmeI GTTT'AAAC
Cuts at: 2689 2689
Size: 5690
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4136 4168 4136
Size: 32 5658
PpuMI rG'GwC_Cy
Cuts at: 693 2097 693
Size: 1404 4286
PspXI vC'TCGA_Gb
Cuts at: 2144 2144
Size: 5690
PstI C_TGCA'G
Cuts at: 2737 3422 2737
Size: 685 5005
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5690
PvuII CAG'CTG
Cuts at: 1569 2377 3115 3475 1569
Size: 808 738 360 3784
Fragments arranged by size:
3784 808 738 360
RsrII CG'GwC_CG
Cuts at: 3885 3885
Size: 5690
SacI G_AGCT'C
Cuts at: 1562 2704 1562
Size: 1142 4548
SalI G'TCGA_C
Cuts at: 2727 2727
Size: 5690
SapI GCTCTTCn'nnn_
Cuts at: 3713 3923 3713
Size: 210 5480
SbfI CC_TGCA'GG
Cuts at: 2737 2737
Size: 5690
ScaI AGT'ACT
Cuts at: 5207 5207
Size: 5690
SexAI A'CCwGG_T
Cuts at: 545 2093 545
Size: 1548 4142
SfcI C'TryA_G
Cuts at: 33 2733 3418 4386 4577 33
Size: 2700 685 968 191 1146
Fragments arranged by size:
2700 1146 968 685 191
SfoI GGC'GCC
Cuts at: 3122 3370 3122
Size: 248 5442
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5690
SmaI CCC'GGG
Cuts at: 2712 2712
Size: 5690
SmlI C'TyrA_G
Cuts at: 1295 2144 2224 2845 3760 4474 4736 1295
Size: 849 80 621 915 714 262 2249
Fragments arranged by size:
2249 915 849 714 621 262 80
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5690
SphI G_CATG'C
Cuts at: 1963 2743 3243 1963
Size: 780 500 4410
Fragments arranged by size:
4410 780 500
StuI AGG'CCT
Cuts at: 2162 2162
Size: 5690
StyI C'CwwG_G
Cuts at: 620 773 807 1751 2824 3178 620
Size: 153 34 944 1073 354 3132
Fragments arranged by size:
3132 1073 944 354 153 34
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 342 765 3273 3406 3865 4074 342
Size: 423 2508 133 459 209 1958
Fragments arranged by size:
2508 1958 459 423 209 133
TatI w'GTAC_w
Cuts at: 1844 2671 5205 1844
Size: 827 2534 2329
Fragments arranged by size:
2534 2329 827
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 770 802 2479 2511 5207 5239 770
Size: 32 1677 32 2696 32 1221
Fragments arranged by size:
2696 1677 1221 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3487 3487
Size: 5690
XbaI T'CTAG_A
Cuts at: 2721 2721
Size: 5690
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1299 1448 1638 3185 1299
Size: 149 190 1547 3804
Fragments arranged by size:
3804 1547 190 149
XhoI C'TCGA_G
Cuts at: 1295 2144 1295
Size: 849 4841
XmaI C'CCGG_G
Cuts at: 2710 2710
Size: 5690
XmnI GAAnn'nnTTC
Cuts at: 1834 1834
Size: 5690
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AleI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NsiI NspI
PacI PasI PciI PmeI PpiI PpuMI PspXI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BglI BmgBI BmtI BsiWI
BsrGI BssHII BstEII BstZ17I ClaI DraIII EcoNI FseI
FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI
NruI PflMI PfoI PmlI PshAI PsiI PspOMI PsrI
SacII SanDI SfiI SgrAI SnaBI SrfI SspI SwaI
ZraI