Restriction map for
pF1KB8642
(Circular) (Six-base) MAPSORT of: pF1KB8642.seq Check: 2481 from: 1 to: 6386
pF1KB8642 6386 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AccI GT'mk_AC
Cuts at: 3164 3424 3164
Size: 260 6126
Acc65I G'GTAC_C
Cuts at: 3402 3402
Size: 6386
AclI AA'CG_TT
Cuts at: 876 3081 6226 876
Size: 2205 3145 1036
Fragments arranged by size:
3145 2205 1036
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1726 2146 2546 3229 4211 4643 4996 5658 1726
Size: 420 400 683 982 432 353 662 2454
Fragments arranged by size:
2454 982 683 662 432 420 400 353
AflII C'TTAA_G
Cuts at: 2497 2497
Size: 6386
AflIII A'CryG_T
Cuts at: 3738 5538 5819 3738
Size: 1800 281 4305
Fragments arranged by size:
4305 1800 281
AlwNI CAG_nnn'CTG
Cuts at: 667 3691 5129 667
Size: 3024 1438 1924
Fragments arranged by size:
3024 1924 1438
ApaLI G'TGCA_C
Cuts at: 5224 5224
Size: 6386
ApoI r'AATT_y
Cuts at: 129 730 1380 2276 2458 2982 3154 3296 3390
Size: 601 650 896 182 524 172 142 94
Cuts at: 3390 5739 5995 129
Size: 2349 256 520
Fragments arranged by size:
2349 896 650 601 524 520 256 182
172 142 94
AseI AT'TA_AT
Cuts at: 20 2468 2720 20
Size: 2448 252 3686
Fragments arranged by size:
3686 2448 252
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6386
AvaI C'yCGr_G
Cuts at: 3406 3406
Size: 6386
BamHI G'GATC_C
Cuts at: 415 3411 415
Size: 2996 3390
BanI G'GyrC_C
Cuts at: 2222 3402 3816 4064 4099 2222
Size: 1180 414 248 35 4509
Fragments arranged by size:
4509 1180 414 248 35
BanII G_rGCy'C
Cuts at: 3400 4430 3400
Size: 1030 5356
BbeI G_GCGC'C
Cuts at: 3820 4068 3820
Size: 248 6138
BbsI GAAGACnn'nnnn_
Cuts at: 209 916 209
Size: 707 5679
BbvCI CC'TCA_GC
Cuts at: 1196 1196
Size: 6386
BciVI GTATCCnnnnn_n'
Cuts at: 2580 4284 5340 6386 2580
Size: 1704 1056 1046 2580
Fragments arranged by size:
2580 1704 1056 1046
BclI T'GATC_A
Cuts at: 633 2031 3906 633
Size: 1398 1875 3113
Fragments arranged by size:
3113 1875 1398
BglII A'GATC_T
Cuts at: 3901 5938 3901
Size: 2037 4349
BlpI GC'TnA_GC
Cuts at: 3498 3498
Size: 6386
Bme1580I G_kGCm'C
Cuts at: 4011 4104 5228 4011
Size: 93 1124 5169
Fragments arranged by size:
5169 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 2872 3598 3679 4014 6158 2872
Size: 726 81 335 2144 3100
Fragments arranged by size:
3100 2144 726 335 81
BmtI G_CTAG'C
Cuts at: 2488 2488
Size: 6386
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4753 4753
Size: 6386
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3047 3092 3047
Size: 45 6341
Bpu10I CC'TnA_GC
Cuts at: 1196 2086 2342 1196
Size: 890 256 5240
Fragments arranged by size:
5240 890 256
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2308 3341 3562 4441 5155 5453 2308
Size: 1033 221 879 714 298 3241
Fragments arranged by size:
3241 1033 879 714 298 221
BsaI GGTCTCn'nnnn_
Cuts at: 2800 6090 2800
Size: 3290 3096
BsaAI yAC'GTr
Cuts at: 3739 3739
Size: 6386
BsaBI GATnn'nnATC
Cuts at: 6 2030 3900 5937 6
Size: 2024 1870 2037 455
Fragments arranged by size:
2037 2024 1870 455
BsaHI Gr'CG_yC
Cuts at: 3817 4065 4767 6263 3817
Size: 248 702 1496 3940
Fragments arranged by size:
3940 1496 702 248
BsaWI w'CCGG_w
Cuts at: 3575 4096 4774 5185 5332 5606 3575
Size: 521 678 411 147 274 4355
Fragments arranged by size:
4355 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2632 2662 2632
Size: 30 6356
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 500 2653 500
Size: 2153 4233
BseYI C'CCAG_C
Cuts at: 3224 3811 4569 5234 5642 3224
Size: 587 758 665 408 3968
Fragments arranged by size:
3968 758 665 587 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 377 1991 377
Size: 1614 4772
BsiEI CG_ry'CG
Cuts at: 104 3928 3974 5204 104
Size: 3824 46 1230 1286
Fragments arranged by size:
3824 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1137 2882 3400 4178 4368 5228 1137
Size: 1745 518 778 190 860 2295
Fragments arranged by size:
2295 1745 860 778 518 190
BsmI GAATG_Cn'
Cuts at: 984 1640 984
Size: 656 5730
Bsp1286I G_dGCh'C
Cuts at: 1137 2882 3400 4011 4104 4178 4368 4430 5228
Size: 1745 518 611 93 74 190 62 798
Cuts at: 5228 1137
Size: 2295
Fragments arranged by size:
2295 1745 798 611 518 190 93 74
62
BspHI T'CATG_A
Cuts at: 737 1751 4818 737
Size: 1014 3067 2305
Fragments arranged by size:
3067 2305 1014
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1041 3436 3650 3952 4333 1041
Size: 2395 214 302 381 3094
Fragments arranged by size:
3094 2395 381 302 214
BsrBI CCG'CTC
Cuts at: 50 834 4678 4732 6216 6381 50
Size: 784 3844 54 1484 165 55
Fragments arranged by size:
3844 1484 784 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3334 4298 3334
Size: 964 5422
BsrFI r'CCGG_y
Cuts at: 4384 4565 4384
Size: 181 6205
BssSI C'ACGA_G
Cuts at: 603 1130 1967 4657 5365 603
Size: 527 837 2690 708 1624
Fragments arranged by size:
2690 1624 837 708 527
BstAPI GCAn_nnn'nTGC
Cuts at: 2752 3763 2752
Size: 1011 5375
BstBI TT'CG_AA
Cuts at: 4747 4747
Size: 6386
BstYI r'GATC_y
Cuts at: 1 415 3411 3878 3901 4236 4482 4788 4800
Size: 414 2996 467 23 335 246 306 12
Cuts at: 4800 4886 5938 1
Size: 86 1052 449
Fragments arranged by size:
2996 1052 467 449 414 335 306 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 593 3194 107
Size: 486 2601 3299
Fragments arranged by size:
3299 2601 486
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3108 4336 4517 4680 3108
Size: 1228 181 163 4814
Fragments arranged by size:
4814 1228 181 163
BtsI GCAGTG_nn'
Cuts at: 956 3629 956
Size: 2673 3713
ClaI AT'CG_AT
Cuts at: 2724 2724
Size: 6386
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 327 362 327
Size: 35 6351
DraI TTT'AAA
Cuts at: 425 501 2843 3385 425
Size: 76 2342 542 3426
Fragments arranged by size:
3426 2342 542 76
DraIII CAC_nnn'GTG
Cuts at: 3098 3098
Size: 6386
DrdI GACnn_nn'nnGTC
Cuts at: 4092 5436 4092
Size: 1344 5042
EaeI y'GGCC_r
Cuts at: 110 1783 3971 4145 4536 4563 5908 110
Size: 1673 2188 174 391 27 1345 588
Fragments arranged by size:
2188 1673 1345 588 391 174 27
EagI C'GGCC_G
Cuts at: 3971 3971
Size: 6386
EarI CTCTTCn'nnn_
Cuts at: 346 387 608 824 1112 1268 1571 4409 4619
Size: 41 221 216 288 156 303 2838 210
Cuts at: 4619 346
Size: 2113
Fragments arranged by size:
2838 2113 303 288 221 216 210 156
41
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4594 5336 5482 6196 4594
Size: 742 146 714 4784
Fragments arranged by size:
4784 742 714 146
EcoICRI GAG'CTC
Cuts at: 3398 3398
Size: 6386
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1726 2146 2546 3047 3092 3229 4211 4643 4996
Size: 420 400 501 45 137 982 432 353
Cuts at: 4996 5658 1726
Size: 662 2454
Fragments arranged by size:
2454 982 662 501 432 420 400 353
137 45
EcoNI CCTnn'n_nnAGG
Cuts at: 3029 3320 3029
Size: 291 6095
EcoO109I rG'GnC_Cy
Cuts at: 1181 1624 1653 1737 1775 3525 1181
Size: 443 29 84 38 1750 4042
Fragments arranged by size:
4042 1750 443 84 38 29
EcoRI G'AATT_C
Cuts at: 3296 3390 3296
Size: 94 6292
EcoRV GAT'ATC
Cuts at: 462 462
Size: 6386
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 581 714 853 4405 581
Size: 133 139 3552 2562
Fragments arranged by size:
3552 2562 139 133
FspI TGC'GCA
Cuts at: 4167 4167
Size: 6386
HaeII r_GCGC'y
Cuts at: 855 3820 4068 5298 6069 855
Size: 2965 248 1230 771 1172
Fragments arranged by size:
2965 1230 1172 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 980 1012 1476 1508 2142 2174 2632 2664 3227
Size: 32 464 32 634 32 458 32 563
Cuts at: 3227 3259 4228 4258 4260 4290 980
Size: 32 969 30 2 30 3076
Fragments arranged by size:
3076 969 634 563 464 458 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 321 3425 321
Size: 3104 3282
HindIII A'AGCT_T
Cuts at: 2012 2012
Size: 6386
KasI G'GCGC_C
Cuts at: 3816 4064 3816
Size: 248 6138
KpnI G_GTAC'C
Cuts at: 3406 3406
Size: 6386
MfeI C'AATT_G
Cuts at: 973 973
Size: 6386
MluI A'CGCG_T
Cuts at: 5819 5819
Size: 6386
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 222 1298 1885 5145 5329 222
Size: 1076 587 3260 184 1279
Fragments arranged by size:
3260 1279 1076 587 184
MscI TGG'CCA
Cuts at: 112 4147 112
Size: 4035 2351
MslI CAynn'nnrTG
Cuts at: 649 1140 1721 1727 2996 3172 4375 4502 649
Size: 491 581 6 1269 176 1203 127 2533
Fragments arranged by size:
2533 1269 1203 581 491 176 127 6
MspA1I CmG'CkG
Cuts at: 2961 3497 3811 4171 4955 5200 2961
Size: 536 314 360 784 245 4147
Fragments arranged by size:
4147 784 536 360 314 245
NaeI GCC'GGC
Cuts at: 4567 4567
Size: 6386
NarI GG'CG_CC
Cuts at: 3817 4065 3817
Size: 248 6138
NcoI C'CATG_G
Cuts at: 107 3194 107
Size: 3087 3299
NdeI CA'TA_TG
Cuts at: 2381 2381
Size: 6386
NgoMIV G'CCGG_C
Cuts at: 4565 4565
Size: 6386
NheI G'CTAG_C
Cuts at: 2484 2484
Size: 6386
NspI r_CATG'y
Cuts at: 1480 2755 3439 3939 5542 1480
Size: 1275 684 500 1603 2324
Fragments arranged by size:
2324 1603 1275 684 500
PciI A'CATG_T
Cuts at: 5538 5538
Size: 6386
PflMI CCAn_nnn'nTGG
Cuts at: 1302 1302
Size: 6386
PmeI GTTT'AAAC
Cuts at: 3385 3385
Size: 6386
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4832 4864 4832
Size: 32 6354
PpuMI rG'GwC_Cy
Cuts at: 1653 1737 1775 1653
Size: 84 38 6264
Fragments arranged by size:
6264 84 38
PsiI TTA'TAA
Cuts at: 569 2237 569
Size: 1668 4718
PstI C_TGCA'G
Cuts at: 234 459 1703 3433 4118 234
Size: 225 1244 1730 685 2502
Fragments arranged by size:
2502 1730 1244 685 225
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6386
PvuII CAG'CTG
Cuts at: 3811 4171 3811
Size: 360 6026
RsrII CG'GwC_CG
Cuts at: 4581 4581
Size: 6386
SacI G_AGCT'C
Cuts at: 3400 3400
Size: 6386
SalI G'TCGA_C
Cuts at: 3423 3423
Size: 6386
SanDI GG'GwC_CC
Cuts at: 1653 1653
Size: 6386
SapI GCTCTTCn'nnn_
Cuts at: 387 608 824 1268 1571 4409 4619 387
Size: 221 216 444 303 2838 210 2154
Fragments arranged by size:
2838 2154 444 303 221 216 210
SbfI CC_TGCA'GG
Cuts at: 3433 3433
Size: 6386
ScaI AGT'ACT
Cuts at: 362 1290 5903 362
Size: 928 4613 845
Fragments arranged by size:
4613 928 845
SfcI C'TryA_G
Cuts at: 33 230 455 1619 1699 1961 2312 3429 4114
Size: 197 225 1164 80 262 351 1117 685
Cuts at: 4114 5082 5273 33
Size: 968 191 1146
Fragments arranged by size:
1164 1146 1117 968 685 351 262 225
197 191 80
SfoI GGC'GCC
Cuts at: 3818 4066 3818
Size: 248 6138
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6386
SmaI CCC'GGG
Cuts at: 3408 3408
Size: 6386
SmlI C'TyrA_G
Cuts at: 2287 2497 3356 3541 4456 5170 5432 2287
Size: 210 859 185 915 714 262 3241
Fragments arranged by size:
3241 915 859 714 262 210 185
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6386
SphI G_CATG'C
Cuts at: 1480 3439 3939 1480
Size: 1959 500 3927
Fragments arranged by size:
3927 1959 500
SspI AAT'ATT
Cuts at: 2268 2268
Size: 6386
StuI AGG'CCT
Cuts at: 1363 1705 1363
Size: 342 6044
StyI C'CwwG_G
Cuts at: 107 3194 3520 3874 107
Size: 3087 326 354 2619
Fragments arranged by size:
3087 2619 354 326
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 370 2010 3969 4102 4561 4770 370
Size: 1640 1959 133 459 209 1986
Fragments arranged by size:
1986 1959 1640 459 209 133
TatI w'GTAC_w
Cuts at: 360 901 1079 1288 5901 360
Size: 541 178 209 4613 845
Fragments arranged by size:
4613 845 541 209 178
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5903 5935 5903
Size: 32 6354
Tth111I GACn'n_nGTC
Cuts at: 4183 4183
Size: 6386
XbaI T'CTAG_A
Cuts at: 3417 3417
Size: 6386
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3881 3881
Size: 6386
XmaI C'CCGG_G
Cuts at: 3406 3406
Size: 6386
XmnI GAAnn'nnTTC
Cuts at: 878 1406 878
Size: 528 5858
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BciVI BclI BglII BlpI Bme1580I BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NspI PciI PflMI PmeI PpiI PpuMI PsiI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AgeI AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BcgI BfrBI BglI BmgBI
BsiWI BsmBI BspEI BsrGI BssHII BstEII BstXI BstZ17I
Bsu36I FseI FspAI HpaI NotI NruI NsiI PacI
PasI PfoI PmlI PshAI PspOMI PspXI PsrI SacII
SexAI SfiI SgrAI SnaBI SrfI SwaI XhoI ZraI