Restriction map for
pF1KB8643
(Circular) (Six-base) MAPSORT of: pF1KB8643.seq Check: 1811 from: 1 to: 4664
pF1KB8643 4664 bp
With 182 enzymes: SgfI *
March 2, 2010 14:29 ..
AatII G_ACGT'C
Cuts at: 1151 1151
Size: 4664
AccI GT'mk_AC
Cuts at: 836 1702 836
Size: 866 3798
Acc65I G'GTAC_C
Cuts at: 1680 1680
Size: 4664
AclI AA'CG_TT
Cuts at: 4504 4504
Size: 4664
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 451 995 2489 2921 3668 3936 451
Size: 544 1494 432 747 268 1179
Fragments arranged by size:
1494 1179 747 544 432 268
AfeI AGC'GCT
Cuts at: 674 674
Size: 4664
AflIII A'CryG_T
Cuts at: 179 551 1472 2016 3120 4097 179
Size: 372 921 544 1104 977 746
Fragments arranged by size:
1104 977 921 746 544 372
AgeI A'CCGG_T
Cuts at: 3052 3052
Size: 4664
AleI CACnn'nnGTG
Cuts at: 262 262
Size: 4664
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 737 737
Size: 4664
AlwNI CAG_nnn'CTG
Cuts at: 1557 1969 3536 1557
Size: 412 1567 2685
Fragments arranged by size:
2685 1567 412
ApaLI G'TGCA_C
Cuts at: 1315 3434 1315
Size: 2119 2545
ApoI r'AATT_y
Cuts at: 1668 4017 4273 1668
Size: 2349 256 2059
Fragments arranged by size:
2349 2059 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4664
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4664
AvaI C'yCGr_G
Cuts at: 162 484 1684 162
Size: 322 1200 3142
Fragments arranged by size:
3142 1200 322
BamHI G'GATC_C
Cuts at: 1689 1689
Size: 4664
BanI G'GyrC_C
Cuts at: 187 1322 1395 1680 2094 2342 2377 187
Size: 1135 73 285 414 248 35 2474
Fragments arranged by size:
2474 1135 414 285 248 73 35
BanII G_rGCy'C
Cuts at: 350 973 1678 2708 350
Size: 623 705 1030 2306
Fragments arranged by size:
2306 1030 705 623
BbeI G_GCGC'C
Cuts at: 2098 2346 2098
Size: 248 4416
BbsI GAAGACnn'nnnn_
Cuts at: 1095 1095
Size: 4664
BciVI GTATCCnnnnn_n'
Cuts at: 1051 2562 3323 4664 1051
Size: 1511 761 1341 1051
Fragments arranged by size:
1511 1341 1051 761
BclI T'GATC_A
Cuts at: 2184 2184
Size: 4664
BglI GCCn_nnn'nGGC
Cuts at: 361 361
Size: 4664
BglII A'GATC_T
Cuts at: 2179 4216 2179
Size: 2037 2627
BlpI GC'TnA_GC
Cuts at: 669 676 1776 669
Size: 7 1100 3557
Fragments arranged by size:
3557 1100 7
Bme1580I G_kGCm'C
Cuts at: 192 1163 1319 1400 2289 2382 3438 192
Size: 971 156 81 889 93 1056 1418
Fragments arranged by size:
1418 1056 971 889 156 93 81
BmgBI CAC'GTC
Cuts at: 554 800 554
Size: 246 4418
BmrI ACTGGGnnnn_n'
Cuts at: 597 1581 1876 1957 2292 4436 597
Size: 984 295 81 335 2144 825
Fragments arranged by size:
2144 984 825 335 295 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 516 3031 516
Size: 2515 2149
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 841 1534 841
Size: 693 3971
Bpu10I CC'TnA_GC
Cuts at: 232 779 232
Size: 547 4117
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 763 1603 1840 2719 3211 3509 763
Size: 840 237 879 492 298 1918
Fragments arranged by size:
1918 879 840 492 298 237
BsaI GGTCTCn'nnnn_
Cuts at: 837 4368 837
Size: 3531 1133
BsaAI yAC'GTr
Cuts at: 180 1473 2017 180
Size: 1293 544 2827
Fragments arranged by size:
2827 1293 544
BsaBI GATnn'nnATC
Cuts at: 6 2178 4215 6
Size: 2172 2037 455
Fragments arranged by size:
2172 2037 455
BsaHI Gr'CG_yC
Cuts at: 1148 1637 2095 2343 3045 4541 1148
Size: 489 458 248 702 1496 1271
Fragments arranged by size:
1496 1271 702 489 458 248
BsaWI w'CCGG_w
Cuts at: 769 839 1443 1853 2374 3052 3326 3473 3884
Size: 70 604 410 521 678 274 147 411
Cuts at: 3884 769
Size: 1549
Fragments arranged by size:
1549 678 604 521 411 410 274 147
70
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 799 1144 799
Size: 345 4319
BseYI C'CCAG_C
Cuts at: 191 328 508 633 652 1047 2089 2847 3424
Size: 137 180 125 19 395 1042 758 577
Cuts at: 3424 3920 191
Size: 496 935
Fragments arranged by size:
1042 935 758 577 496 395 180 137
125 19
BsiEI CG_ry'CG
Cuts at: 104 2206 2252 3460 104
Size: 2102 46 1208 1308
Fragments arranged by size:
2102 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 350 896 1319 1678 2456 2646 3438 350
Size: 546 423 359 778 190 792 1576
Fragments arranged by size:
1576 792 778 546 423 359 190
BsmI GAATG_Cn'
Cuts at: 1226 1226
Size: 4664
BsmBI CGTCTCn'nnnn_
Cuts at: 388 1155 388
Size: 767 3897
Bsp1286I G_dGCh'C
Cuts at: 192 350 896 973 1163 1319 1400 1678 2289
Size: 158 546 77 190 156 81 278 611
Cuts at: 2289 2382 2456 2646 2708 3438 192
Size: 93 74 190 62 730 1418
Fragments arranged by size:
1418 730 611 546 278 190 190 158
156 93 81 77 74 62
BspEI T'CCGG_A
Cuts at: 769 3884 769
Size: 3115 1549
BspHI T'CATG_A
Cuts at: 3840 3840
Size: 4664
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1358 1388 1565 1714 1928 2230 2611 1358
Size: 30 177 149 214 302 381 3411
Fragments arranged by size:
3411 381 302 214 177 149 30
BsrBI CCG'CTC
Cuts at: 50 2956 3010 4494 4659 50
Size: 2906 54 1484 165 55
Fragments arranged by size:
2906 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2576 2576
Size: 4664
BsrFI r'CCGG_y
Cuts at: 724 1319 2662 2843 3052 724
Size: 595 1343 181 209 2336
Fragments arranged by size:
2336 1343 595 209 181
BssSI C'ACGA_G
Cuts at: 1291 1489 2935 3293 1291
Size: 198 1446 358 2662
Fragments arranged by size:
2662 1446 358 198
BstAPI GCAn_nnn'nTGC
Cuts at: 571 2041 571
Size: 1470 3194
BstBI TT'CG_AA
Cuts at: 3025 3025
Size: 4664
BstEII G'GTnAC_C
Cuts at: 817 817
Size: 4664
BstXI CCAn_nnnn'nTGG
Cuts at: 800 800
Size: 4664
BstYI r'GATC_y
Cuts at: 1 944 1019 1689 2156 2179 2514 2760 3772
Size: 943 75 670 467 23 335 246 1012
Cuts at: 3772 3858 3870 4216 1
Size: 86 12 346 449
Fragments arranged by size:
1012 943 670 467 449 346 335 246
86 75 23 12
BtgI C'CryG_G
Cuts at: 107 239 356 107
Size: 132 117 4415
Fragments arranged by size:
4415 132 117
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 329 593 2614 2795 2958 329
Size: 264 2021 181 163 2035
Fragments arranged by size:
2035 2021 264 181 163
BtsI GCAGTG_nn'
Cuts at: 1907 1907
Size: 4664
DraI TTT'AAA
Cuts at: 1663 1663
Size: 4664
DraIII CAC_nnn'GTG
Cuts at: 1341 1341
Size: 4664
DrdI GACnn_nn'nnGTC
Cuts at: 2370 3228 2370
Size: 858 3806
EaeI y'GGCC_r
Cuts at: 110 242 353 398 620 1601 2249 2423 2814
Size: 132 111 45 222 981 648 174 391
Cuts at: 2814 2841 4186 110
Size: 27 1345 588
Fragments arranged by size:
1345 981 648 588 391 222 174 132
111 45 27
EagI C'GGCC_G
Cuts at: 2249 2249
Size: 4664
EarI CTCTTCn'nnn_
Cuts at: 1549 2687 2897 1549
Size: 1138 210 3316
Fragments arranged by size:
3316 1138 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 379 1030 1405 2872 3182 3328 4474 379
Size: 651 375 1467 310 146 1146 569
Fragments arranged by size:
1467 1146 651 569 375 310 146
EcoICRI GAG'CTC
Cuts at: 348 1676 348
Size: 1328 3336
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 451 841 995 1534 2489 2921 3668 3936 451
Size: 390 154 539 955 432 747 268 1179
Fragments arranged by size:
1179 955 747 539 432 390 268 154
EcoO109I rG'GnC_Cy
Cuts at: 599 1803 599
Size: 1204 3460
EcoRI G'AATT_C
Cuts at: 1668 1668
Size: 4664
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2683 2683
Size: 4664
FspI TGC'GCA
Cuts at: 2445 2445
Size: 4664
HaeII r_GCGC'y
Cuts at: 676 2098 2346 3368 4347 676
Size: 1422 248 1022 979 993
Fragments arranged by size:
1422 1022 993 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2506 2536 2538 2568 2506
Size: 30 2 30 4602
Fragments arranged by size:
4602 30 30 2
HincII GTy'rAC
Cuts at: 1703 1703
Size: 4664
KasI G'GCGC_C
Cuts at: 2094 2342 2094
Size: 248 4416
KpnI G_GTAC'C
Cuts at: 1684 1684
Size: 4664
MluI A'CGCG_T
Cuts at: 4097 4097
Size: 4664
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3335 3519 3335
Size: 184 4480
MscI TGG'CCA
Cuts at: 112 355 2425 112
Size: 243 2070 2351
Fragments arranged by size:
2351 2070 243
MslI CAynn'nnrTG
Cuts at: 182 262 1574 2653 2780 182
Size: 80 1312 1079 127 2066
Fragments arranged by size:
2066 1312 1079 127 80
MspA1I CmG'CkG
Cuts at: 429 512 717 1367 1775 2089 2449 3462 3707
Size: 83 205 650 408 314 360 1013 245
Cuts at: 3707 429
Size: 1386
Fragments arranged by size:
1386 1013 650 408 360 314 245 205
83
NaeI GCC'GGC
Cuts at: 726 2845 726
Size: 2119 2545
NarI GG'CG_CC
Cuts at: 2095 2343 2095
Size: 248 4416
NcoI C'CATG_G
Cuts at: 107 356 107
Size: 249 4415
NdeI CA'TA_TG
Cuts at: 1030 1030
Size: 4664
NgoMIV G'CCGG_C
Cuts at: 724 2843 724
Size: 2119 2545
NspI r_CATG'y
Cuts at: 1717 2217 3124 1717
Size: 500 907 3257
Fragments arranged by size:
3257 907 500
PciI A'CATG_T
Cuts at: 3120 3120
Size: 4664
PflMI CCAn_nnn'nTGG
Cuts at: 1054 1054
Size: 4664
PfoI T'CCnGG_A
Cuts at: 290 290
Size: 4664
PmeI GTTT'AAAC
Cuts at: 1663 1663
Size: 4664
PmlI CAC'GTG
Cuts at: 180 180
Size: 4664
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3803 3835 3803
Size: 32 4632
PpuMI rG'GwC_Cy
Cuts at: 599 599
Size: 4664
PstI C_TGCA'G
Cuts at: 452 567 596 992 1355 1711 2396 452
Size: 115 29 396 363 356 685 2720
Fragments arranged by size:
2720 685 396 363 356 115 29
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4664
PvuII CAG'CTG
Cuts at: 717 2089 2449 717
Size: 1372 360 2932
Fragments arranged by size:
2932 1372 360
RsrII CG'GwC_CG
Cuts at: 2859 2859
Size: 4664
SacI G_AGCT'C
Cuts at: 350 1678 350
Size: 1328 3336
SalI G'TCGA_C
Cuts at: 1701 1701
Size: 4664
SapI GCTCTTCn'nnn_
Cuts at: 1549 2687 2897 1549
Size: 1138 210 3316
Fragments arranged by size:
3316 1138 210
SbfI CC_TGCA'GG
Cuts at: 1711 1711
Size: 4664
ScaI AGT'ACT
Cuts at: 4181 4181
Size: 4664
SfcI C'TryA_G
Cuts at: 33 448 563 592 988 1351 1707 2392 3385
Size: 415 115 29 396 363 356 685 993
Cuts at: 3385 3576 33
Size: 191 1121
Fragments arranged by size:
1121 993 685 415 396 363 356 191
115 29
SfoI GGC'GCC
Cuts at: 2096 2344 2096
Size: 248 4416
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4664
SmaI CCC'GGG
Cuts at: 164 1686 164
Size: 1522 3142
SmlI C'TyrA_G
Cuts at: 742 1618 1819 2734 3226 3488 742
Size: 876 201 915 492 262 1918
Fragments arranged by size:
1918 915 876 492 262 201
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4664
SphI G_CATG'C
Cuts at: 1717 2217 1717
Size: 500 4164
SspI AAT'ATT
Cuts at: 877 877
Size: 4664
StuI AGG'CCT
Cuts at: 691 1609 691
Size: 918 3746
StyI C'CwwG_G
Cuts at: 107 356 995 1798 2152 107
Size: 249 639 803 354 2619
Fragments arranged by size:
2619 803 639 354 249
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 525 625 1061 2247 2380 2839 3048 525
Size: 100 436 1186 133 459 209 2141
Fragments arranged by size:
2141 1186 459 436 209 133 100
TatI w'GTAC_w
Cuts at: 4179 4179
Size: 4664
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 543 575 4181 4213 543
Size: 32 3606 32 994
Fragments arranged by size:
3606 994 32 32
Tth111I GACn'n_nGTC
Cuts at: 2461 2461
Size: 4664
XbaI T'CTAG_A
Cuts at: 1695 1695
Size: 4664
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 181 630 1051 2159 181
Size: 449 421 1108 2686
Fragments arranged by size:
2686 1108 449 421
XmaI C'CCGG_G
Cuts at: 162 1684 162
Size: 1522 3142
XmnI GAAnn'nnTTC
Cuts at: 133 133
Size: 4664
ZraI GAC'GTC
Cuts at: 1149 1149
Size: 4664
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NspI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AflII AhdI AloI ApaI AscI AvrII BaeI
BbvCI BcgI BfrBI BmtI BsaXI BsgI BsiWI BsrGI
BssHII BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NheI NotI NruI NsiI
PacI PasI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SwaI XhoI