Restriction map for
pF1KB8647
(Circular) (Six-base) MAPSORT of: pF1KB8647.seq Check: 564 from: 1 to: 5585
pF1KB8647 5585 bp
With 182 enzymes: SgfI *
March 2, 2010 14:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 535 833 535
Size: 298 5287
AccI GT'mk_AC
Cuts at: 1875 2531 2623 1875
Size: 656 92 4837
Fragments arranged by size:
4837 656 92
Acc65I G'GTAC_C
Cuts at: 2601 2601
Size: 5585
AclI AA'CG_TT
Cuts at: 5425 5425
Size: 5585
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 805 3410 3842 4589 4857 805
Size: 2605 432 747 268 1533
Fragments arranged by size:
2605 1533 747 432 268
AflIII A'CryG_T
Cuts at: 2278 2937 4041 5018 2278
Size: 659 1104 977 2845
Fragments arranged by size:
2845 1104 977 659
AgeI A'CCGG_T
Cuts at: 3973 3973
Size: 5585
AhdI GACnn_n'nnGTC
Cuts at: 1489 1489
Size: 5585
AleI CACnn'nnGTG
Cuts at: 943 1883 2548 943
Size: 940 665 3980
Fragments arranged by size:
3980 940 665
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1612 2320 1612
Size: 708 4877
AlwNI CAG_nnn'CTG
Cuts at: 600 2340 2890 4457 600
Size: 1740 550 1567 1728
Fragments arranged by size:
1740 1728 1567 550
ApaI G_GGCC'C
Cuts at: 835 920 1409 1798 1896 835
Size: 85 489 389 98 4524
Fragments arranged by size:
4524 489 389 98 85
ApaLI G'TGCA_C
Cuts at: 1269 1420 4355 1269
Size: 151 2935 2499
Fragments arranged by size:
2935 2499 151
ApoI r'AATT_y
Cuts at: 726 2589 4938 5194 726
Size: 1863 2349 256 1117
Fragments arranged by size:
2349 1863 1117 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5585
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5585
AvaI C'yCGr_G
Cuts at: 221 556 583 676 2605 221
Size: 335 27 93 1929 3201
Fragments arranged by size:
3201 1929 335 93 27
AvrII C'CTAG_G
Cuts at: 306 306
Size: 5585
BamHI G'GATC_C
Cuts at: 1343 2561 2610 1343
Size: 1218 49 4318
Fragments arranged by size:
4318 1218 49
BanI G'GyrC_C
Cuts at: 240 821 1184 1194 1665 2203 2218 2601 3015
Size: 581 363 10 471 538 15 383 414
Cuts at: 3015 3263 3298 240
Size: 248 35 2527
Fragments arranged by size:
2527 581 538 471 414 383 363 248
35 15 10
BanII G_rGCy'C
Cuts at: 455 583 835 920 971 1409 1798 1896 2174
Size: 128 252 85 51 438 389 98 278
Cuts at: 2174 2599 3629 455
Size: 425 1030 2411
Fragments arranged by size:
2411 1030 438 425 389 278 252 128
98 85 51
BbeI G_GCGC'C
Cuts at: 2207 2222 3019 3267 2207
Size: 15 797 248 4525
Fragments arranged by size:
4525 797 248 15
BbsI GAAGACnn'nnnn_
Cuts at: 1065 1065
Size: 5585
BbvCI CC'TCA_GC
Cuts at: 1132 1132
Size: 5585
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 5551
BciVI GTATCCnnnnn_n'
Cuts at: 3483 4244 5585 3483
Size: 761 1341 3483
Fragments arranged by size:
3483 1341 761
BclI T'GATC_A
Cuts at: 2552 3105 2552
Size: 553 5032
BfrBI ATG'CAT
Cuts at: 1589 1589
Size: 5585
BglI GCCn_nnn'nGGC
Cuts at: 1003 1526 1793 2227 1003
Size: 523 267 434 4361
Fragments arranged by size:
4361 523 434 267
BglII A'GATC_T
Cuts at: 1391 3100 5137 1391
Size: 1709 2037 1839
Fragments arranged by size:
2037 1839 1709
BlpI GC'TnA_GC
Cuts at: 577 2697 577
Size: 2120 3465
Bme1580I G_kGCm'C
Cuts at: 245 835 920 1199 1273 1409 1424 1798 1896
Size: 590 85 279 74 136 15 374 98
Cuts at: 1896 3210 3303 4359 245
Size: 1314 93 1056 1471
Fragments arranged by size:
1471 1314 1056 590 374 279 136 98
93 85 74 15
BmgBI CAC'GTC
Cuts at: 909 2085 909
Size: 1176 4409
BmrI ACTGGGnnnn_n'
Cuts at: 2797 2878 3213 5357 2797
Size: 81 335 2144 3025
Fragments arranged by size:
3025 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1483 3952 1483
Size: 2469 3116
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 474 792 883 943 1478 1774 1785 1846 2033
Size: 318 91 60 535 296 11 61 187
Cuts at: 2033 474
Size: 4026
Fragments arranged by size:
4026 535 318 296 187 91 61 60
11
Bpu10I CC'TnA_GC
Cuts at: 1132 1132
Size: 5585
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 430 2761 3640 4132 4430 430
Size: 2331 879 492 298 1585
Fragments arranged by size:
2331 1585 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 5289 5289
Size: 5585
BsaAI yAC'GTr
Cuts at: 759 2938 759
Size: 2179 3406
BsaBI GATnn'nnATC
Cuts at: 6 1372 3099 5136 6
Size: 1366 1727 2037 455
Fragments arranged by size:
2037 1727 1366 455
BsaHI Gr'CG_yC
Cuts at: 988 1127 2204 2219 2371 2528 3016 3264 3966
Size: 139 1077 15 152 157 488 248 702
Cuts at: 3966 5462 988
Size: 1496 1111
Fragments arranged by size:
1496 1111 1077 702 488 248 157 152
139 15
BsaWI w'CCGG_w
Cuts at: 1700 2774 3295 3973 4247 4394 4805 1700
Size: 1074 521 678 274 147 411 2480
Fragments arranged by size:
2480 1074 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 472 478 502 508 604 634 790 820 1293
Size: 6 24 6 96 30 156 30 473
Cuts at: 1293 1323 1369 1399 472
Size: 30 46 30 4658
Fragments arranged by size:
4658 473 156 96 46 30 30 30
30 24 6 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 625 1260 1783 625
Size: 635 523 4427
Fragments arranged by size:
4427 635 523
BseYI C'CCAG_C
Cuts at: 735 1150 1910 2198 2492 3010 3768 4345 4841
Size: 415 760 288 294 518 758 577 496
Cuts at: 4841 735
Size: 1479
Fragments arranged by size:
1479 760 758 577 518 496 415 294
288
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 613 1012 1081 2032 2164 2341 2364 613
Size: 399 69 951 132 177 23 3834
Fragments arranged by size:
3834 951 399 177 132 69 23
BsiEI CG_ry'CG
Cuts at: 104 1010 1364 2126 3127 3173 4381 104
Size: 906 354 762 1001 46 1208 1308
Fragments arranged by size:
1308 1208 1001 906 762 354 46
BsiHKAI G_wGCw'C
Cuts at: 455 563 1055 1273 1424 1775 2599 3377 3567
Size: 108 492 218 151 351 824 778 190
Cuts at: 3567 4359 455
Size: 792 1681
Fragments arranged by size:
1681 824 792 778 492 351 218 190
151 108
Bsp1286I G_dGCh'C
Cuts at: 245 455 563 583 835 920 971 1055 1199
Size: 210 108 20 252 85 51 84 144
Cuts at: 1199 1273 1409 1424 1775 1798 1896 2174 2599
Size: 74 136 15 351 23 98 278 425
Cuts at: 2599 3210 3303 3377 3567 3629 4359 245
Size: 611 93 74 190 62 730 1471
Fragments arranged by size:
1471 730 611 425 351 278 252 210
190 144 136 108 98 93 85 84
74 74 62 51 23 20 15
BspEI T'CCGG_A
Cuts at: 4805 4805
Size: 5585
BspHI T'CATG_A
Cuts at: 4761 4761
Size: 5585
BspMI ACCTGCnnnn'nnnn_
Cuts at: 535 833 2635 2849 3151 3532 535
Size: 298 1802 214 302 381 2588
Fragments arranged by size:
2588 1802 381 302 298 214
BsrBI CCG'CTC
Cuts at: 50 3877 3931 5415 5580 50
Size: 3827 54 1484 165 55
Fragments arranged by size:
3827 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3497 3497
Size: 5585
BsrFI r'CCGG_y
Cuts at: 566 1065 2389 2510 3583 3764 3973 566
Size: 499 1324 121 1073 181 209 2178
Fragments arranged by size:
2178 1324 1073 499 209 181 121
BsrGI T'GTAC_A
Cuts at: 1433 1433
Size: 5585
BssHII G'CGCG_C
Cuts at: 2152 2152
Size: 5585
BssSI C'ACGA_G
Cuts at: 268 3856 4214 268
Size: 3588 358 1639
Fragments arranged by size:
3588 1639 358
BstAPI GCAn_nnn'nTGC
Cuts at: 600 2962 600
Size: 2362 3223
BstBI TT'CG_AA
Cuts at: 3946 3946
Size: 5585
BstEII G'GTnAC_C
Cuts at: 2574 2574
Size: 5585
BstXI CCAn_nnnn'nTGG
Cuts at: 114 136 1530 114
Size: 22 1394 4169
Fragments arranged by size:
4169 1394 22
BstYI r'GATC_y
Cuts at: 1 384 1343 1391 2561 2610 3077 3100 3435
Size: 383 959 48 1170 49 467 23 335
Cuts at: 3435 3681 4693 4779 4791 5137 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1170 1012 959 467 449 383 346 335
246 86 49 48 23 12
BtgI C'CryG_G
Cuts at: 113 971 1349 1358 1846 1958 113
Size: 858 378 9 488 112 3740
Fragments arranged by size:
3740 858 488 378 112 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1884 3535 3716 3879 1884
Size: 1651 181 163 3590
Fragments arranged by size:
3590 1651 181 163
BtsI GCAGTG_nn'
Cuts at: 423 598 1173 1594 1657 1735 1929 2181 2828
Size: 175 575 421 63 78 194 252 647
Cuts at: 2828 423
Size: 3180
Fragments arranged by size:
3180 647 575 421 252 194 175 78
63
DraI TTT'AAA
Cuts at: 2584 2584
Size: 5585
DraIII CAC_nnn'GTG
Cuts at: 2112 2461 2112
Size: 349 5236
DrdI GACnn_nn'nnGTC
Cuts at: 3291 4149 3291
Size: 858 4727
EaeI y'GGCC_r
Cuts at: 116 896 911 1355 1361 1843 2123 2463 2508
Size: 780 15 444 6 482 280 340 45
Cuts at: 2508 3170 3344 3735 3762 5107 116
Size: 662 174 391 27 1345 594
Fragments arranged by size:
1345 780 662 594 482 444 391 340
280 174 45 27 15 6
EagI C'GGCC_G
Cuts at: 1361 2123 3170 1361
Size: 762 1047 3776
Fragments arranged by size:
3776 1047 762
EarI CTCTTCn'nnn_
Cuts at: 922 1640 2057 3608 3818 922
Size: 718 417 1551 210 2689
Fragments arranged by size:
2689 1551 718 417 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 223 334 2245 3793 4103 4249 5395 223
Size: 111 1911 1548 310 146 1146 413
Fragments arranged by size:
1911 1548 1146 413 310 146 111
EcoICRI GAG'CTC
Cuts at: 453 2597 453
Size: 2144 3441
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 474 792 805 883 943 1478 1774 1785 1846
Size: 318 13 78 60 535 296 11 61
Cuts at: 1846 2033 3410 3842 4589 4857 474
Size: 187 1377 432 747 268 1202
Fragments arranged by size:
1377 1202 747 535 432 318 296 268
187 78 61 60 13 11
EcoNI CCTnn'n_nnAGG
Cuts at: 1984 1984
Size: 5585
EcoO109I rG'GnC_Cy
Cuts at: 123 517 681 774 790 831 832 840 917
Size: 394 164 93 16 41 1 8 77
Cuts at: 917 1023 1794 1795 1892 1893 2337 2496 2724
Size: 106 771 1 97 1 444 159 228
Cuts at: 2724 123
Size: 2984
Fragments arranged by size:
2984 771 444 394 228 164 159 106
97 93 77 41 16 8 1 1
1
EcoRI G'AATT_C
Cuts at: 726 2589 726
Size: 1863 3722
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3604 3604
Size: 5585
FseI GG_CCGG'CC
Cuts at: 2514 2514
Size: 5585
FspI TGC'GCA
Cuts at: 1318 3366 1318
Size: 2048 3537
HaeII r_GCGC'y
Cuts at: 2092 2207 2222 3019 3267 4289 5268 2092
Size: 115 15 797 248 1022 979 2409
Fragments arranged by size:
2409 1022 979 797 248 115 15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 604 636 1475 1507 3427 3457 3459 3489 604
Size: 32 839 32 1920 30 2 30 2700
Fragments arranged by size:
2700 1920 839 32 32 30 30 2
HincII GTy'rAC
Cuts at: 630 1876 2624 630
Size: 1246 748 3591
Fragments arranged by size:
3591 1246 748
KasI G'GCGC_C
Cuts at: 2203 2218 3015 3263 2203
Size: 15 797 248 4525
Fragments arranged by size:
4525 797 248 15
KpnI G_GTAC'C
Cuts at: 2605 2605
Size: 5585
MluI A'CGCG_T
Cuts at: 5018 5018
Size: 5585
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1424 4256 4440 1424
Size: 2832 184 2569
Fragments arranged by size:
2832 2569 184
MscI TGG'CCA
Cuts at: 898 1357 1845 3346 898
Size: 459 488 1501 3137
Fragments arranged by size:
3137 1501 488 459
MslI CAynn'nnrTG
Cuts at: 112 943 1566 1883 2548 3574 3701 112
Size: 831 623 317 665 1026 127 1996
Fragments arranged by size:
1996 1026 831 665 623 317 127
MspA1I CmG'CkG
Cuts at: 812 1910 1924 2442 2468 2492 2558 2696 3010
Size: 1098 14 518 26 24 66 138 314
Cuts at: 3010 3370 4383 4628 812
Size: 360 1013 245 1769
Fragments arranged by size:
1769 1098 1013 518 360 314 245 138
66 26 24 14
NaeI GCC'GGC
Cuts at: 568 2391 2512 3766 568
Size: 1823 121 1254 2387
Fragments arranged by size:
2387 1823 1254 121
NarI GG'CG_CC
Cuts at: 2204 2219 3016 3264 2204
Size: 15 797 248 4525
Fragments arranged by size:
4525 797 248 15
NcoI C'CATG_G
Cuts at: 1846 1846
Size: 5585
NgoMIV G'CCGG_C
Cuts at: 566 2389 2510 3764 566
Size: 1823 121 1254 2387
Fragments arranged by size:
2387 1823 1254 121
NsiI A_TGCA'T
Cuts at: 1591 1591
Size: 5585
NspI r_CATG'y
Cuts at: 1589 2282 2382 2638 3138 4045 1589
Size: 693 100 256 500 907 3129
Fragments arranged by size:
3129 907 693 500 256 100
PasI CC'CwG_GG
Cuts at: 1122 1275 1122
Size: 153 5432
PciI A'CATG_T
Cuts at: 2278 4041 2278
Size: 1763 3822
PflMI CCAn_nnn'nTGG
Cuts at: 2112 2571 2112
Size: 459 5126
PfoI T'CCnGG_A
Cuts at: 692 1937 692
Size: 1245 4340
PmeI GTTT'AAAC
Cuts at: 2584 2584
Size: 5585
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 172 204 4724 4756 172
Size: 32 4520 32 1001
Fragments arranged by size:
4520 1001 32 32
PpuMI rG'GwC_Cy
Cuts at: 517 774 840 2337 517
Size: 257 66 1497 3765
Fragments arranged by size:
3765 1497 257 66
PspOMI G'GGCC_C
Cuts at: 831 916 1405 1794 1892 831
Size: 85 489 389 98 4524
Fragments arranged by size:
4524 489 389 98 85
PstI C_TGCA'G
Cuts at: 830 958 1328 1753 2099 2309 2632 3317 830
Size: 128 370 425 346 210 323 685 3098
Fragments arranged by size:
3098 685 425 370 346 323 210 128
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5585
PvuII CAG'CTG
Cuts at: 812 1924 2492 2558 3010 3370 812
Size: 1112 568 66 452 360 3027
Fragments arranged by size:
3027 1112 568 452 360 66
RsrII CG'GwC_CG
Cuts at: 3780 3780
Size: 5585
SacI G_AGCT'C
Cuts at: 455 2599 455
Size: 2144 3441
SalI G'TCGA_C
Cuts at: 1874 2622 1874
Size: 748 4837
SanDI GG'GwC_CC
Cuts at: 517 517
Size: 5585
SapI GCTCTTCn'nnn_
Cuts at: 1640 3608 3818 1640
Size: 1968 210 3407
Fragments arranged by size:
3407 1968 210
SbfI CC_TGCA'GG
Cuts at: 830 2632 830
Size: 1802 3783
ScaI AGT'ACT
Cuts at: 5102 5102
Size: 5585
SexAI A'CCwGG_T
Cuts at: 2363 2363
Size: 5585
SfcI C'TryA_G
Cuts at: 33 651 750 826 954 1324 1749 2095 2305
Size: 618 99 76 128 370 425 346 210
Cuts at: 2305 2628 3313 4306 4497 33
Size: 323 685 993 191 1121
Fragments arranged by size:
1121 993 685 618 425 370 346 323
210 191 128 99 76
SfiI GGCCn_nnn'nGGCC
Cuts at: 1793 1793
Size: 5585
SfoI GGC'GCC
Cuts at: 2205 2220 3017 3265 2205
Size: 15 797 248 4525
Fragments arranged by size:
4525 797 248 15
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5585
SmaI CCC'GGG
Cuts at: 223 2607 223
Size: 2384 3201
SmlI C'TyrA_G
Cuts at: 445 2740 3655 4147 4409 445
Size: 2295 915 492 262 1621
Fragments arranged by size:
2295 1621 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5585
SphI G_CATG'C
Cuts at: 2382 2638 3138 2382
Size: 256 500 4829
Fragments arranged by size:
4829 500 256
StuI AGG'CCT
Cuts at: 196 1081 196
Size: 885 4700
StyI C'CwwG_G
Cuts at: 306 836 1601 1846 2265 2719 3073 306
Size: 530 765 245 419 454 354 2818
Fragments arranged by size:
2818 765 530 454 419 354 245
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 905 1288 1902 3168 3301 3760 3969 905
Size: 383 614 1266 133 459 209 2521
Fragments arranged by size:
2521 1266 614 459 383 209 133
TatI w'GTAC_w
Cuts at: 341 360 1433 5100 341
Size: 19 1073 3667 826
Fragments arranged by size:
3667 1073 826 19
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 790 822 1293 1325 5102 5134 790
Size: 32 471 32 3777 32 1241
Fragments arranged by size:
3777 1241 471 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3382 3382
Size: 5585
XbaI T'CTAG_A
Cuts at: 2616 2616
Size: 5585
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 468 906 3080 468
Size: 438 2174 2973
Fragments arranged by size:
2973 2174 438
XmaI C'CCGG_G
Cuts at: 221 2605 221
Size: 2384 3201
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FseI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AloI AscI BaeI BmtI BsiWI
BsmI BsmBI BstZ17I Bsu36I ClaI CspCI EcoRV FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI PacI
PmlI PshAI PsiI PspXI PsrI SacII SgrAI SnaBI
SrfI SspI SwaI XhoI XmnI ZraI