Restriction map for
pF1KB8648
(Circular) (Six-base) MAPSORT of: pF1KB8648.seq Check: 5703 from: 1 to: 4133
pF1KB8648 4133 bp
With 182 enzymes: SgfI *
March 2, 2010 14:29 ..
AccI GT'mk_AC
Cuts at: 258 317 644 1171 258
Size: 59 327 527 3220
Fragments arranged by size:
3220 527 327 59
Acc65I G'GTAC_C
Cuts at: 1149 1149
Size: 4133
AclI AA'CG_TT
Cuts at: 3973 3973
Size: 4133
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1958 2390 3137 3405 1958
Size: 432 747 268 2686
Fragments arranged by size:
2686 747 432 268
AflIII A'CryG_T
Cuts at: 1485 2589 3566 1485
Size: 1104 977 2052
Fragments arranged by size:
2052 1104 977
AgeI A'CCGG_T
Cuts at: 2521 2521
Size: 4133
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 128 160 128
Size: 32 4101
AlwNI CAG_nnn'CTG
Cuts at: 550 1438 3005 550
Size: 888 1567 1678
Fragments arranged by size:
1678 1567 888
ApaLI G'TGCA_C
Cuts at: 2903 2903
Size: 4133
ApoI r'AATT_y
Cuts at: 427 487 1137 3486 3742 427
Size: 60 650 2349 256 818
Fragments arranged by size:
2349 818 650 256 60
AseI AT'TA_AT
Cuts at: 20 20
Size: 4133
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4133
AvaI C'yCGr_G
Cuts at: 1153 1153
Size: 4133
BamHI G'GATC_C
Cuts at: 1158 1158
Size: 4133
BanI G'GyrC_C
Cuts at: 1149 1563 1811 1846 1149
Size: 414 248 35 3436
Fragments arranged by size:
3436 414 248 35
BanII G_rGCy'C
Cuts at: 282 1037 1147 2177 282
Size: 755 110 1030 2238
Fragments arranged by size:
2238 1030 755 110
BbeI G_GCGC'C
Cuts at: 1567 1815 1567
Size: 248 3885
BciVI GTATCCnnnnn_n'
Cuts at: 2031 2792 4133 2031
Size: 761 1341 2031
Fragments arranged by size:
2031 1341 761
BclI T'GATC_A
Cuts at: 1653 1653
Size: 4133
BglII A'GATC_T
Cuts at: 1648 3685 1648
Size: 2037 2096
BlpI GC'TnA_GC
Cuts at: 1245 1245
Size: 4133
Bme1580I G_kGCm'C
Cuts at: 1758 1851 2907 1758
Size: 93 1056 2984
Fragments arranged by size:
2984 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1345 1426 1761 3905 1345
Size: 81 335 2144 1573
Fragments arranged by size:
2144 1573 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1051 2500 1051
Size: 1449 2684
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1124 1124
Size: 4133
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1309 2188 2680 2978 1309
Size: 879 492 298 2464
Fragments arranged by size:
2464 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3837 3837
Size: 4133
BsaAI yAC'GTr
Cuts at: 682 1486 682
Size: 804 3329
BsaBI GATnn'nnATC
Cuts at: 6 1647 3684 6
Size: 1641 2037 455
Fragments arranged by size:
2037 1641 455
BsaHI Gr'CG_yC
Cuts at: 1564 1812 2514 4010 1564
Size: 248 702 1496 1687
Fragments arranged by size:
1687 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1322 1843 2521 2795 2942 3353 1322
Size: 521 678 274 147 411 2102
Fragments arranged by size:
2102 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1043 1043
Size: 4133
BseYI C'CCAG_C
Cuts at: 506 1558 2316 2893 3389 506
Size: 1052 758 577 496 1250
Fragments arranged by size:
1250 1052 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1675 1721 2929 104
Size: 1571 46 1208 1308
Fragments arranged by size:
1571 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 282 1037 1147 1925 2115 2907 282
Size: 755 110 778 190 792 1508
Fragments arranged by size:
1508 792 778 755 190 110
Bsp1286I G_dGCh'C
Cuts at: 282 1037 1147 1758 1851 1925 2115 2177 2907
Size: 755 110 611 93 74 190 62 730
Cuts at: 2907 282
Size: 1508
Fragments arranged by size:
1508 755 730 611 190 110 93 74
62
BspEI T'CCGG_A
Cuts at: 3353 3353
Size: 4133
BspHI T'CATG_A
Cuts at: 3309 3309
Size: 4133
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1183 1397 1699 2080 1183
Size: 214 302 381 3236
Fragments arranged by size:
3236 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2425 2479 3963 4128 50
Size: 2375 54 1484 165 55
Fragments arranged by size:
2375 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 294 623 2045 294
Size: 329 1422 2382
Fragments arranged by size:
2382 1422 329
BsrFI r'CCGG_y
Cuts at: 2131 2312 2521 2131
Size: 181 209 3743
Fragments arranged by size:
3743 209 181
BssSI C'ACGA_G
Cuts at: 2404 2762 2404
Size: 358 3775
BstAPI GCAn_nnn'nTGC
Cuts at: 1099 1510 1099
Size: 411 3722
BstBI TT'CG_AA
Cuts at: 2494 2494
Size: 4133
BstYI r'GATC_y
Cuts at: 1 954 1158 1625 1648 1983 2229 3241 3327
Size: 953 204 467 23 335 246 1012 86
Cuts at: 3327 3339 3685 1
Size: 12 346 449
Fragments arranged by size:
1012 953 467 449 346 335 246 204
86 23 12
BstZ17I GTA'TAC
Cuts at: 259 259
Size: 4133
Bsu36I CC'TnA_GG
Cuts at: 616 616
Size: 4133
BtgI C'CryG_G
Cuts at: 813 813
Size: 4133
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2083 2264 2427 2083
Size: 181 163 3789
Fragments arranged by size:
3789 181 163
BtsI GCAGTG_nn'
Cuts at: 1376 1376
Size: 4133
ClaI AT'CG_AT
Cuts at: 773 773
Size: 4133
DraI TTT'AAA
Cuts at: 1132 1132
Size: 4133
DrdI GACnn_nn'nnGTC
Cuts at: 1839 2697 1839
Size: 858 3275
EaeI y'GGCC_r
Cuts at: 810 824 1718 1892 2283 2310 3655 810
Size: 14 894 174 391 27 1345 1288
Fragments arranged by size:
1345 1288 894 391 174 27 14
EagI C'GGCC_G
Cuts at: 1718 1718
Size: 4133
EarI CTCTTCn'nnn_
Cuts at: 149 270 1020 2156 2366 149
Size: 121 750 1136 210 1916
Fragments arranged by size:
1916 1136 750 210 121
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 145 2341 2651 2797 3943 145
Size: 2196 310 146 1146 335
Fragments arranged by size:
2196 1146 335 310 146
EcoICRI GAG'CTC
Cuts at: 280 1035 1145 280
Size: 755 110 3268
Fragments arranged by size:
3268 755 110
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1124 1958 2390 3137 3405 1124
Size: 834 432 747 268 1852
Fragments arranged by size:
1852 834 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1272 1272
Size: 4133
EcoRI G'AATT_C
Cuts at: 1137 1137
Size: 4133
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 594 2152 594
Size: 1558 2575
FspI TGC'GCA
Cuts at: 1914 1914
Size: 4133
HaeII r_GCGC'y
Cuts at: 1567 1815 2837 3816 1567
Size: 248 1022 979 1884
Fragments arranged by size:
1884 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 909 941 1975 2005 2007 2037 909
Size: 32 1034 30 2 30 3005
Fragments arranged by size:
3005 1034 32 30 30 2
HincII GTy'rAC
Cuts at: 1172 1172
Size: 4133
HindIII A'AGCT_T
Cuts at: 476 1011 476
Size: 535 3598
KasI G'GCGC_C
Cuts at: 1563 1811 1563
Size: 248 3885
KpnI G_GTAC'C
Cuts at: 1153 1153
Size: 4133
MluI A'CGCG_T
Cuts at: 3566 3566
Size: 4133
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2804 2988 2804
Size: 184 3949
MscI TGG'CCA
Cuts at: 1894 1894
Size: 4133
MslI CAynn'nnrTG
Cuts at: 2122 2249 2122
Size: 127 4006
MspA1I CmG'CkG
Cuts at: 1244 1558 1918 2931 3176 1244
Size: 314 360 1013 245 2201
Fragments arranged by size:
2201 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2314 2314
Size: 4133
NarI GG'CG_CC
Cuts at: 1564 1812 1564
Size: 248 3885
NgoMIV G'CCGG_C
Cuts at: 2312 2312
Size: 4133
NspI r_CATG'y
Cuts at: 1186 1686 2593 1186
Size: 500 907 2726
Fragments arranged by size:
2726 907 500
PciI A'CATG_T
Cuts at: 2589 2589
Size: 4133
PflMI CCAn_nnn'nTGG
Cuts at: 550 550
Size: 4133
PfoI T'CCnGG_A
Cuts at: 543 927 543
Size: 384 3749
PmeI GTTT'AAAC
Cuts at: 1132 1132
Size: 4133
PmlI CAC'GTG
Cuts at: 682 682
Size: 4133
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3272 3304 3272
Size: 32 4101
PstI C_TGCA'G
Cuts at: 1180 1865 1180
Size: 685 3448
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4133
PvuII CAG'CTG
Cuts at: 1558 1918 1558
Size: 360 3773
RsrII CG'GwC_CG
Cuts at: 2328 2328
Size: 4133
SacI G_AGCT'C
Cuts at: 282 1037 1147 282
Size: 755 110 3268
Fragments arranged by size:
3268 755 110
SalI G'TCGA_C
Cuts at: 1170 1170
Size: 4133
SapI GCTCTTCn'nnn_
Cuts at: 270 2156 2366 270
Size: 1886 210 2037
Fragments arranged by size:
2037 1886 210
SbfI CC_TGCA'GG
Cuts at: 1180 1180
Size: 4133
ScaI AGT'ACT
Cuts at: 659 3650 659
Size: 2991 1142
SfcI C'TryA_G
Cuts at: 33 1176 1861 2854 3045 33
Size: 1143 685 993 191 1121
Fragments arranged by size:
1143 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1565 1813 1565
Size: 248 3885
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4133
SmaI CCC'GGG
Cuts at: 1155 1155
Size: 4133
SmlI C'TyrA_G
Cuts at: 1288 2203 2695 2957 1288
Size: 915 492 262 2464
Fragments arranged by size:
2464 915 492 262
SpeI A'CTAG_T
Cuts at: 67 691 67
Size: 624 3509
SphI G_CATG'C
Cuts at: 1186 1686 1186
Size: 500 3633
StyI C'CwwG_G
Cuts at: 1121 1267 1621 1121
Size: 146 354 3633
Fragments arranged by size:
3633 354 146
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1716 1849 2308 2517 1716
Size: 133 459 209 3332
Fragments arranged by size:
3332 459 209 133
TatI w'GTAC_w
Cuts at: 657 3648 657
Size: 2991 1142
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 191 223 3650 3682 191
Size: 32 3427 32 642
Fragments arranged by size:
3427 642 32 32
Tth111I GACn'n_nGTC
Cuts at: 1930 1930
Size: 4133
XbaI T'CTAG_A
Cuts at: 1164 1164
Size: 4133
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 745 1628 745
Size: 883 3250
XmaI C'CCGG_G
Cuts at: 1153 1153
Size: 4133
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI
PflMI PfoI PmeI PmlI PpiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BsaXI BsgI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstXI CspCI DraIII EcoNI EcoRV
FseI FspAI HpaI MfeI NcoI NdeI NheI NotI
NruI NsiI PacI PasI PpuMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI