Restriction map for
pF1KB8649
(Circular) (Six-base) MAPSORT of: pF1KB8649.seq Check: 5698 from: 1 to: 4169
pF1KB8649 4169 bp
With 182 enzymes: SgfI *
March 2, 2010 14:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 753 807 1015 753
Size: 54 208 3907
Fragments arranged by size:
3907 208 54
AccI GT'mk_AC
Cuts at: 455 1207 455
Size: 752 3417
Acc65I G'GTAC_C
Cuts at: 1185 1185
Size: 4169
AclI AA'CG_TT
Cuts at: 4009 4009
Size: 4169
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 523 854 925 1994 2426 3173 3441 523
Size: 331 71 1069 432 747 268 1251
Fragments arranged by size:
1251 1069 747 432 331 268 71
AfeI AGC'GCT
Cuts at: 967 967
Size: 4169
AflIII A'CryG_T
Cuts at: 1521 2625 3602 1521
Size: 1104 977 2088
Fragments arranged by size:
2088 1104 977
AgeI A'CCGG_T
Cuts at: 2557 2557
Size: 4169
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 947 947
Size: 4169
AlwNI CAG_nnn'CTG
Cuts at: 369 627 1474 3041 369
Size: 258 847 1567 1497
Fragments arranged by size:
1567 1497 847 258
ApaLI G'TGCA_C
Cuts at: 2939 2939
Size: 4169
ApoI r'AATT_y
Cuts at: 507 634 1173 3522 3778 507
Size: 127 539 2349 256 898
Fragments arranged by size:
2349 898 539 256 127
AseI AT'TA_AT
Cuts at: 20 20
Size: 4169
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4169
AvaI C'yCGr_G
Cuts at: 198 840 1189 198
Size: 642 349 3178
Fragments arranged by size:
3178 642 349
BamHI G'GATC_C
Cuts at: 1194 1194
Size: 4169
BanI G'GyrC_C
Cuts at: 735 984 1185 1599 1847 1882 735
Size: 249 201 414 248 35 3022
Fragments arranged by size:
3022 414 249 248 201 35
BanII G_rGCy'C
Cuts at: 528 1183 2213 528
Size: 655 1030 2484
Fragments arranged by size:
2484 1030 655
BbeI G_GCGC'C
Cuts at: 1603 1851 1603
Size: 248 3921
BbsI GAAGACnn'nnnn_
Cuts at: 913 913
Size: 4169
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 363 397 90
Size: 34 239 34 3862
Fragments arranged by size:
3862 239 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2067 2828 4169 2067
Size: 761 1341 2067
Fragments arranged by size:
2067 1341 761
BclI T'GATC_A
Cuts at: 1689 1689
Size: 4169
BfrBI ATG'CAT
Cuts at: 1062 1062
Size: 4169
BglII A'GATC_T
Cuts at: 1684 3721 1684
Size: 2037 2132
BlpI GC'TnA_GC
Cuts at: 418 1075 1281 418
Size: 657 206 3306
Fragments arranged by size:
3306 657 206
Bme1580I G_kGCm'C
Cuts at: 740 1794 1887 2943 740
Size: 1054 93 1056 1966
Fragments arranged by size:
1966 1056 1054 93
BmrI ACTGGGnnnn_n'
Cuts at: 1381 1462 1797 3941 1381
Size: 81 335 2144 1609
Fragments arranged by size:
2144 1609 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2536 2536
Size: 4169
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 997 997
Size: 4169
Bpu10I CC'TnA_GC
Cuts at: 853 853
Size: 4169
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1120 1345 2224 2716 3014 1120
Size: 225 879 492 298 2275
Fragments arranged by size:
2275 879 492 298 225
BsaI GGTCTCn'nnnn_
Cuts at: 280 490 1140 3873 280
Size: 210 650 2733 576
Fragments arranged by size:
2733 650 576 210
BsaAI yAC'GTr
Cuts at: 1522 1522
Size: 4169
BsaBI GATnn'nnATC
Cuts at: 6 1683 3720 6
Size: 1677 2037 455
Fragments arranged by size:
2037 1677 455
BsaHI Gr'CG_yC
Cuts at: 1022 1600 1848 2550 4046 1022
Size: 578 248 702 1496 1145
Fragments arranged by size:
1496 1145 702 578 248
BsaWI w'CCGG_w
Cuts at: 131 308 1358 1879 2557 2831 2978 3389 131
Size: 177 1050 521 678 274 147 411 911
Fragments arranged by size:
1050 911 678 521 411 274 177 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 592 798 1132 592
Size: 206 334 3629
Fragments arranged by size:
3629 334 206
BseYI C'CCAG_C
Cuts at: 369 958 1594 2352 2929 3425 369
Size: 589 636 758 577 496 1113
Fragments arranged by size:
1113 758 636 589 577 496
BsiEI CG_ry'CG
Cuts at: 104 1711 1757 2965 104
Size: 1607 46 1208 1308
Fragments arranged by size:
1607 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 785 854 1183 1961 2151 2943 785
Size: 69 329 778 190 792 2011
Fragments arranged by size:
2011 792 778 329 190 69
BsmBI CGTCTCn'nnnn_
Cuts at: 1114 1114
Size: 4169
Bsp1286I G_dGCh'C
Cuts at: 528 740 785 854 1183 1794 1887 1961 2151
Size: 212 45 69 329 611 93 74 190
Cuts at: 2151 2213 2943 528
Size: 62 730 1754
Fragments arranged by size:
1754 730 611 329 212 190 93 74
69 62 45
BspEI T'CCGG_A
Cuts at: 131 308 3389 131
Size: 177 3081 911
Fragments arranged by size:
3081 911 177
BspHI T'CATG_A
Cuts at: 569 3345 569
Size: 2776 1393
BspMI ACCTGCnnnn'nnnn_
Cuts at: 753 807 1015 1219 1433 1735 2116 753
Size: 54 208 204 214 302 381 2806
Fragments arranged by size:
2806 381 302 214 208 204 54
BsrBI CCG'CTC
Cuts at: 50 2461 2515 3999 4164 50
Size: 2411 54 1484 165 55
Fragments arranged by size:
2411 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2081 2081
Size: 4169
BsrFI r'CCGG_y
Cuts at: 2167 2348 2557 2167
Size: 181 209 3779
Fragments arranged by size:
3779 209 181
BssSI C'ACGA_G
Cuts at: 786 2440 2798 786
Size: 1654 358 2157
Fragments arranged by size:
2157 1654 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1546 1546
Size: 4169
BstBI TT'CG_AA
Cuts at: 2530 2530
Size: 4169
BstYI r'GATC_y
Cuts at: 1 830 1194 1661 1684 2019 2265 3277 3363
Size: 829 364 467 23 335 246 1012 86
Cuts at: 3363 3375 3721 1
Size: 12 346 449
Fragments arranged by size:
1012 829 467 449 364 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 176 989 176
Size: 813 3356
BtgI C'CryG_G
Cuts at: 542 794 801 542
Size: 252 7 3910
Fragments arranged by size:
3910 252 7
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1156 2119 2300 2463 1156
Size: 963 181 163 2862
Fragments arranged by size:
2862 963 181 163
BtsI GCAGTG_nn'
Cuts at: 1412 1412
Size: 4169
DraI TTT'AAA
Cuts at: 1168 1168
Size: 4169
DraIII CAC_nnn'GTG
Cuts at: 984 984
Size: 4169
DrdI GACnn_nn'nnGTC
Cuts at: 157 1875 2733 157
Size: 1718 858 1593
Fragments arranged by size:
1718 1593 858
EaeI y'GGCC_r
Cuts at: 1754 1928 2319 2346 3691 1754
Size: 174 391 27 1345 2232
Fragments arranged by size:
2232 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1754 1754
Size: 4169
EarI CTCTTCn'nnn_
Cuts at: 2192 2402 2192
Size: 210 3959
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2377 2687 2833 3979 2377
Size: 310 146 1146 2567
Fragments arranged by size:
2567 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1181 1181
Size: 4169
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 523 854 925 997 1994 2426 3173 3441 523
Size: 331 71 72 997 432 747 268 1251
Fragments arranged by size:
1251 997 747 432 331 268 72 71
EcoO109I rG'GnC_Cy
Cuts at: 257 1308 257
Size: 1051 3118
EcoRI G'AATT_C
Cuts at: 1173 1173
Size: 4169
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2188 2188
Size: 4169
FspI TGC'GCA
Cuts at: 1950 1950
Size: 4169
HaeII r_GCGC'y
Cuts at: 394 969 1603 1851 2873 3852 394
Size: 575 634 248 1022 979 711
Fragments arranged by size:
1022 979 711 634 575 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1096 1128 2011 2041 2043 2073 1096
Size: 32 883 30 2 30 3192
Fragments arranged by size:
3192 883 32 30 30 2
HincII GTy'rAC
Cuts at: 1208 1208
Size: 4169
KasI G'GCGC_C
Cuts at: 1599 1847 1599
Size: 248 3921
KpnI G_GTAC'C
Cuts at: 1189 1189
Size: 4169
MluI A'CGCG_T
Cuts at: 3602 3602
Size: 4169
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2840 3024 2840
Size: 184 3985
MscI TGG'CCA
Cuts at: 1930 1930
Size: 4169
MslI CAynn'nnrTG
Cuts at: 347 2158 2285 347
Size: 1811 127 2231
Fragments arranged by size:
2231 1811 127
MspA1I CmG'CkG
Cuts at: 189 445 654 803 1280 1594 1954 2967 3212
Size: 256 209 149 477 314 360 1013 245
Cuts at: 3212 189
Size: 1146
Fragments arranged by size:
1146 1013 477 360 314 256 245 209
149
NaeI GCC'GGC
Cuts at: 2350 2350
Size: 4169
NarI GG'CG_CC
Cuts at: 1600 1848 1600
Size: 248 3921
NgoMIV G'CCGG_C
Cuts at: 2348 2348
Size: 4169
NsiI A_TGCA'T
Cuts at: 1064 1064
Size: 4169
NspI r_CATG'y
Cuts at: 1066 1222 1722 2629 1066
Size: 156 500 907 2606
Fragments arranged by size:
2606 907 500 156
PciI A'CATG_T
Cuts at: 2625 2625
Size: 4169
PfoI T'CCnGG_A
Cuts at: 476 476
Size: 4169
PmeI GTTT'AAAC
Cuts at: 1168 1168
Size: 4169
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 820 852 3308 3340 820
Size: 32 2456 32 1649
Fragments arranged by size:
2456 1649 32 32
PpuMI rG'GwC_Cy
Cuts at: 257 257
Size: 4169
PstI C_TGCA'G
Cuts at: 776 818 1130 1216 1901 776
Size: 42 312 86 685 3044
Fragments arranged by size:
3044 685 312 86 42
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4169
PvuII CAG'CTG
Cuts at: 189 445 654 1594 1954 189
Size: 256 209 940 360 2404
Fragments arranged by size:
2404 940 360 256 209
RsrII CG'GwC_CG
Cuts at: 2364 2364
Size: 4169
SacI G_AGCT'C
Cuts at: 1183 1183
Size: 4169
SacII CC_GC'GG
Cuts at: 804 804
Size: 4169
SalI G'TCGA_C
Cuts at: 1206 1206
Size: 4169
SanDI GG'GwC_CC
Cuts at: 257 257
Size: 4169
SapI GCTCTTCn'nnn_
Cuts at: 2192 2402 2192
Size: 210 3959
SbfI CC_TGCA'GG
Cuts at: 818 1216 818
Size: 398 3771
ScaI AGT'ACT
Cuts at: 3686 3686
Size: 4169
SfcI C'TryA_G
Cuts at: 33 772 814 1050 1126 1212 1897 2890 3081
Size: 739 42 236 76 86 685 993 191
Cuts at: 3081 33
Size: 1121
Fragments arranged by size:
1121 993 739 685 236 191 86 76
42
SfoI GGC'GCC
Cuts at: 1601 1849 1601
Size: 248 3921
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4169
SmaI CCC'GGG
Cuts at: 842 1191 842
Size: 349 3820
SmlI C'TyrA_G
Cuts at: 1135 1324 2239 2731 2993 1135
Size: 189 915 492 262 2311
Fragments arranged by size:
2311 915 492 262 189
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4169
SphI G_CATG'C
Cuts at: 1066 1222 1722 1066
Size: 156 500 3513
Fragments arranged by size:
3513 500 156
StuI AGG'CCT
Cuts at: 1111 1111
Size: 4169
StyI C'CwwG_G
Cuts at: 269 1303 1657 269
Size: 1034 354 2781
Fragments arranged by size:
2781 1034 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1752 1885 2344 2553 1752
Size: 133 459 209 3368
Fragments arranged by size:
3368 459 209 133
TatI w'GTAC_w
Cuts at: 239 3684 239
Size: 3445 724
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 775 807 3686 3718 775
Size: 32 2879 32 1226
Fragments arranged by size:
2879 1226 32 32
Tth111I GACn'n_nGTC
Cuts at: 1966 1966
Size: 4169
XbaI T'CTAG_A
Cuts at: 1200 1200
Size: 4169
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1664 1664
Size: 4169
XmaI C'CCGG_G
Cuts at: 840 1189 840
Size: 349 3820
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NsiI NspI PciI PfoI PmeI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AhdI AleI AloI ApaI AscI AvrII
BaeI BbvCI BglI BmgBI BmtI BsaXI BsgI BsiWI
BsmI BsrGI BssHII BstEII BstXI BstZ17I ClaI CspCI
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NcoI
NdeI NheI NotI NruI PacI PasI PflMI PmlI
PshAI PsiI PspOMI PspXI PsrI SexAI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI XmnI ZraI