Restriction map for
pF1KB8650
(Circular) (Six-base) MAPSORT of: pF1KB8650.seq Check: 9401 from: 1 to: 4103
pF1KB8650 4103 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AccI GT'mk_AC
Cuts at: 494 1141 494
Size: 647 3456
Acc65I G'GTAC_C
Cuts at: 1119 1119
Size: 4103
AclI AA'CG_TT
Cuts at: 3943 3943
Size: 4103
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 125 1928 2360 2713 3375 125
Size: 1803 432 353 662 853
Fragments arranged by size:
1803 853 662 432 353
AflIII A'CryG_T
Cuts at: 1455 3255 3536 1455
Size: 1800 281 2022
Fragments arranged by size:
2022 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 594 594
Size: 4103
AleI CACnn'nnGTG
Cuts at: 617 617
Size: 4103
AlwNI CAG_nnn'CTG
Cuts at: 747 1408 2846 747
Size: 661 1438 2004
Fragments arranged by size:
2004 1438 661
ApaI G_GGCC'C
Cuts at: 936 936
Size: 4103
ApaLI G'TGCA_C
Cuts at: 2941 2941
Size: 4103
ApoI r'AATT_y
Cuts at: 252 510 1107 3456 3712 252
Size: 258 597 2349 256 643
Fragments arranged by size:
2349 643 597 258 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4103
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4103
AvaI C'yCGr_G
Cuts at: 554 935 1094 1123 554
Size: 381 159 29 3534
Fragments arranged by size:
3534 381 159 29
BamHI G'GATC_C
Cuts at: 1128 1128
Size: 4103
BanI G'GyrC_C
Cuts at: 841 1083 1119 1533 1781 1816 841
Size: 242 36 414 248 35 3128
Fragments arranged by size:
3128 414 248 242 36 35
BanII G_rGCy'C
Cuts at: 370 936 1117 2147 370
Size: 566 181 1030 2326
Fragments arranged by size:
2326 1030 566 181
BbeI G_GCGC'C
Cuts at: 1537 1785 1537
Size: 248 3855
BbsI GAAGACnn'nnnn_
Cuts at: 566 566
Size: 4103
BciVI GTATCCnnnnn_n'
Cuts at: 2001 3057 4103 2001
Size: 1056 1046 2001
Fragments arranged by size:
2001 1056 1046
BclI T'GATC_A
Cuts at: 1623 1623
Size: 4103
BfrBI ATG'CAT
Cuts at: 879 879
Size: 4103
BglI GCCn_nnn'nGGC
Cuts at: 809 809
Size: 4103
BglII A'GATC_T
Cuts at: 533 1618 3655 533
Size: 1085 2037 981
Fragments arranged by size:
2037 1085 981
BlpI GC'TnA_GC
Cuts at: 1215 1215
Size: 4103
Bme1580I G_kGCm'C
Cuts at: 248 936 1728 1821 2945 248
Size: 688 792 93 1124 1406
Fragments arranged by size:
1406 1124 792 688 93
BmrI ACTGGGnnnn_n'
Cuts at: 892 1315 1396 1731 3875 892
Size: 423 81 335 2144 1120
Fragments arranged by size:
2144 1120 423 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2470 2470
Size: 4103
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 191 504 892 982 191
Size: 313 388 90 3312
Fragments arranged by size:
3312 388 313 90
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1279 2158 2872 3170 1279
Size: 879 714 298 2212
Fragments arranged by size:
2212 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 3807 3807
Size: 4103
BsaAI yAC'GTr
Cuts at: 1080 1456 1080
Size: 376 3727
BsaBI GATnn'nnATC
Cuts at: 6 1617 3654 6
Size: 1611 2037 455
Fragments arranged by size:
2037 1611 455
BsaHI Gr'CG_yC
Cuts at: 1534 1782 2484 3980 1534
Size: 248 702 1496 1657
Fragments arranged by size:
1657 1496 702 248
BsaWI w'CCGG_w
Cuts at: 590 656 1292 1813 2491 2902 3049 3323 590
Size: 66 636 521 678 411 147 274 1370
Fragments arranged by size:
1370 678 636 521 411 274 147 66
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 217 821 217
Size: 604 3499
BseYI C'CCAG_C
Cuts at: 732 740 975 1056 1528 2286 2951 3359 732
Size: 8 235 81 472 758 665 408 1476
Fragments arranged by size:
1476 758 665 472 408 235 81 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 922 1030 922
Size: 108 3995
BsiEI CG_ry'CG
Cuts at: 104 1041 1645 1691 2921 104
Size: 937 604 46 1230 1286
Fragments arranged by size:
1286 1230 937 604 46
BsiHKAI G_wGCw'C
Cuts at: 370 1117 1895 2085 2945 370
Size: 747 778 190 860 1528
Fragments arranged by size:
1528 860 778 747 190
BsmI GAATG_Cn'
Cuts at: 870 870
Size: 4103
Bsp1286I G_dGCh'C
Cuts at: 248 370 936 1117 1728 1821 1895 2085 2147
Size: 122 566 181 611 93 74 190 62
Cuts at: 2147 2945 248
Size: 798 1406
Fragments arranged by size:
1406 798 611 566 190 181 122 93
74 62
BspHI T'CATG_A
Cuts at: 2535 2535
Size: 4103
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1153 1367 1669 2050 1153
Size: 214 302 381 3206
Fragments arranged by size:
3206 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2395 2449 3933 4098 50
Size: 2345 54 1484 165 55
Fragments arranged by size:
2345 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2015 2015
Size: 4103
BsrFI r'CCGG_y
Cuts at: 844 1036 2101 2282 844
Size: 192 1065 181 2665
Fragments arranged by size:
2665 1065 192 181
BssSI C'ACGA_G
Cuts at: 2374 3082 2374
Size: 708 3395
BstAPI GCAn_nnn'nTGC
Cuts at: 1480 1480
Size: 4103
BstBI TT'CG_AA
Cuts at: 2464 2464
Size: 4103
BstEII G'GTnAC_C
Cuts at: 568 568
Size: 4103
BstXI CCAn_nnnn'nTGG
Cuts at: 1031 1080 1031
Size: 49 4054
BstYI r'GATC_y
Cuts at: 1 533 1128 1595 1618 1953 2199 2505 2517
Size: 532 595 467 23 335 246 306 12
Cuts at: 2517 2603 3655 1
Size: 86 1052 449
Fragments arranged by size:
1052 595 532 467 449 335 306 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4103
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2053 2234 2397 2053
Size: 181 163 3759
Fragments arranged by size:
3759 181 163
BtsI GCAGTG_nn'
Cuts at: 134 861 1346 134
Size: 727 485 2891
Fragments arranged by size:
2891 727 485
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 187 222 187
Size: 35 4068
DraI TTT'AAA
Cuts at: 1102 1102
Size: 4103
DrdI GACnn_nn'nnGTC
Cuts at: 1809 3153 1809
Size: 1344 2759
EaeI y'GGCC_r
Cuts at: 1038 1688 1862 2253 2280 3625 1038
Size: 650 174 391 27 1345 1516
Fragments arranged by size:
1516 1345 650 391 174 27
EagI C'GGCC_G
Cuts at: 1038 1688 1038
Size: 650 3453
EarI CTCTTCn'nnn_
Cuts at: 1069 2126 2336 1069
Size: 1057 210 2836
Fragments arranged by size:
2836 1057 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 232 2311 3053 3199 3913 232
Size: 2079 742 146 714 422
Fragments arranged by size:
2079 742 714 422 146
EcoICRI GAG'CTC
Cuts at: 368 1115 368
Size: 747 3356
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 125 191 504 892 982 1928 2360 2713 3375
Size: 66 313 388 90 946 432 353 662
Cuts at: 3375 125
Size: 853
Fragments arranged by size:
946 853 662 432 388 353 313 90
66
EcoO109I rG'GnC_Cy
Cuts at: 596 957 1005 1242 596
Size: 361 48 237 3457
Fragments arranged by size:
3457 361 237 48
EcoRI G'AATT_C
Cuts at: 1107 1107
Size: 4103
EcoRV GAT'ATC
Cuts at: 772 772
Size: 4103
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 552 2122 552
Size: 1570 2533
FspI TGC'GCA
Cuts at: 1884 1884
Size: 4103
HaeII r_GCGC'y
Cuts at: 739 943 1537 1785 3015 3786 739
Size: 204 594 248 1230 771 1056
Fragments arranged by size:
1230 1056 771 594 248 204
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 580 612 623 655 1945 1975 1977 2007 580
Size: 32 11 32 1290 30 2 30 2676
Fragments arranged by size:
2676 1290 32 32 30 30 11 2
HincII GTy'rAC
Cuts at: 1142 1142
Size: 4103
KasI G'GCGC_C
Cuts at: 1533 1781 1533
Size: 248 3855
KpnI G_GTAC'C
Cuts at: 1123 1123
Size: 4103
MluI A'CGCG_T
Cuts at: 3536 3536
Size: 4103
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 647 666 1043 2862 3046 647
Size: 19 377 1819 184 1704
Fragments arranged by size:
1819 1704 377 184 19
MscI TGG'CCA
Cuts at: 1864 1864
Size: 4103
MslI CAynn'nnrTG
Cuts at: 404 617 1029 2092 2219 404
Size: 213 412 1063 127 2288
Fragments arranged by size:
2288 1063 412 213 127
MspA1I CmG'CkG
Cuts at: 747 989 1214 1528 1888 2672 2917 747
Size: 242 225 314 360 784 245 1933
Fragments arranged by size:
1933 784 360 314 245 242 225
NaeI GCC'GGC
Cuts at: 2284 2284
Size: 4103
NarI GG'CG_CC
Cuts at: 1534 1782 1534
Size: 248 3855
NcoI C'CATG_G
Cuts at: 107 107
Size: 4103
NgoMIV G'CCGG_C
Cuts at: 2282 2282
Size: 4103
NsiI A_TGCA'T
Cuts at: 881 881
Size: 4103
NspI r_CATG'y
Cuts at: 1156 1656 3259 1156
Size: 500 1603 2000
Fragments arranged by size:
2000 1603 500
PasI CC'CwG_GG
Cuts at: 373 600 373
Size: 227 3876
PciI A'CATG_T
Cuts at: 3255 3255
Size: 4103
PfoI T'CCnGG_A
Cuts at: 1007 1007
Size: 4103
PmeI GTTT'AAAC
Cuts at: 1102 1102
Size: 4103
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 964 996 2549 2581 964
Size: 32 1553 32 2486
Fragments arranged by size:
2486 1553 32 32
PpuMI rG'GwC_Cy
Cuts at: 596 1005 596
Size: 409 3694
PshAI GACnn'nnGTC
Cuts at: 566 566
Size: 4103
PspOMI G'GGCC_C
Cuts at: 932 932
Size: 4103
PstI C_TGCA'G
Cuts at: 695 752 779 1150 1835 695
Size: 57 27 371 685 2963
Fragments arranged by size:
2963 685 371 57 27
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4103
PvuII CAG'CTG
Cuts at: 747 1528 1888 747
Size: 781 360 2962
Fragments arranged by size:
2962 781 360
RsrII CG'GwC_CG
Cuts at: 2298 2298
Size: 4103
SacI G_AGCT'C
Cuts at: 370 1117 370
Size: 747 3356
SalI G'TCGA_C
Cuts at: 1140 1140
Size: 4103
SapI GCTCTTCn'nnn_
Cuts at: 2126 2336 2126
Size: 210 3893
SbfI CC_TGCA'GG
Cuts at: 1150 1150
Size: 4103
ScaI AGT'ACT
Cuts at: 3620 3620
Size: 4103
SfcI C'TryA_G
Cuts at: 33 501 691 748 775 1146 1831 2799 2990
Size: 468 190 57 27 371 685 968 191
Cuts at: 2990 33
Size: 1146
Fragments arranged by size:
1146 968 685 468 371 191 190 57
27
SfoI GGC'GCC
Cuts at: 1535 1783 1535
Size: 248 3855
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4103
SmaI CCC'GGG
Cuts at: 937 1125 937
Size: 188 3915
SmlI C'TyrA_G
Cuts at: 1258 2173 2887 3149 1258
Size: 915 714 262 2212
Fragments arranged by size:
2212 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4103
SphI G_CATG'C
Cuts at: 1156 1656 1156
Size: 500 3603
SrfI GCCC'GGGC
Cuts at: 937 937
Size: 4103
StuI AGG'CCT
Cuts at: 293 293
Size: 4103
StyI C'CwwG_G
Cuts at: 107 1237 1591 107
Size: 1130 354 2619
Fragments arranged by size:
2619 1130 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1101 1686 1819 2278 2487 1101
Size: 585 133 459 209 2717
Fragments arranged by size:
2717 585 459 209 133
TatI w'GTAC_w
Cuts at: 3618 3618
Size: 4103
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3620 3652 3620
Size: 32 4071
Tth111I GACn'n_nGTC
Cuts at: 1900 1900
Size: 4103
XbaI T'CTAG_A
Cuts at: 1134 1134
Size: 4103
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1598 1598
Size: 4103
XmaI C'CCGG_G
Cuts at: 935 1123 935
Size: 188 3915
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AhdI AleI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NsiI NspI PasI PciI
PfoI PmeI PpiI PpuMI PshAI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SrfI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AlfI AloI AscI
AvrII BaeI BbvCI BcgI BmgBI BmtI Bpu10I BsaXI
BsiWI BsmBI BspEI BsrGI BssHII BstZ17I Bsu36I ClaI
DraIII EcoNI FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI PacI PflMI PmlI PsiI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SspI
SwaI XhoI XmnI ZraI