Restriction map for
pF1KB8651
(Circular) (Six-base) MAPSORT of: pF1KB8651.seq Check: 2330 from: 1 to: 4571
pF1KB8651 4571 bp
With 182 enzymes: SgfI *
March 2, 2010 14:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 780 780
Size: 4571
AccI GT'mk_AC
Cuts at: 1609 1609
Size: 4571
Acc65I G'GTAC_C
Cuts at: 1587 1587
Size: 4571
AclI AA'CG_TT
Cuts at: 4411 4411
Size: 4571
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 763 2396 2828 3575 3843 763
Size: 1633 432 747 268 1491
Fragments arranged by size:
1633 1491 747 432 268
AflII C'TTAA_G
Cuts at: 712 712
Size: 4571
AflIII A'CryG_T
Cuts at: 1923 3027 4004 1923
Size: 1104 977 2490
Fragments arranged by size:
2490 1104 977
AgeI A'CCGG_T
Cuts at: 2959 2959
Size: 4571
AhdI GACnn_n'nnGTC
Cuts at: 1311 1311
Size: 4571
AlwNI CAG_nnn'CTG
Cuts at: 161 1116 1876 3443 161
Size: 955 760 1567 1289
Fragments arranged by size:
1567 1289 955 760
ApaI G_GGCC'C
Cuts at: 297 329 1407 297
Size: 32 1078 3461
Fragments arranged by size:
3461 1078 32
ApaLI G'TGCA_C
Cuts at: 3341 3341
Size: 4571
ApoI r'AATT_y
Cuts at: 1575 3924 4180 1575
Size: 2349 256 1966
Fragments arranged by size:
2349 1966 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4571
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4571
AvaI C'yCGr_G
Cuts at: 400 1149 1380 1591 400
Size: 749 231 211 3380
Fragments arranged by size:
3380 749 231 211
BamHI G'GATC_C
Cuts at: 1596 1596
Size: 4571
BanI G'GyrC_C
Cuts at: 1587 2001 2249 2284 1587
Size: 414 248 35 3874
Fragments arranged by size:
3874 414 248 35
BanII G_rGCy'C
Cuts at: 297 329 1407 1585 2615 297
Size: 32 1078 178 1030 2253
Fragments arranged by size:
2253 1078 1030 178 32
BbeI G_GCGC'C
Cuts at: 2005 2253 2005
Size: 248 4323
BbsI GAAGACnn'nnnn_
Cuts at: 1282 1282
Size: 4571
BbvCI CC'TCA_GC
Cuts at: 509 1042 509
Size: 533 4038
BciVI GTATCCnnnnn_n'
Cuts at: 2469 3230 4571 2469
Size: 761 1341 2469
Fragments arranged by size:
2469 1341 761
BclI T'GATC_A
Cuts at: 2091 2091
Size: 4571
BglI GCCn_nnn'nGGC
Cuts at: 358 358
Size: 4571
BglII A'GATC_T
Cuts at: 2086 4123 2086
Size: 2037 2534
BlpI GC'TnA_GC
Cuts at: 1441 1683 1441
Size: 242 4329
Bme1580I G_kGCm'C
Cuts at: 297 329 763 1407 2196 2289 3345 297
Size: 32 434 644 789 93 1056 1523
Fragments arranged by size:
1523 1056 789 644 434 93 32
BmrI ACTGGGnnnn_n'
Cuts at: 324 762 1158 1783 1864 2199 4343 324
Size: 438 396 625 81 335 2144 552
Fragments arranged by size:
2144 625 552 438 396 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2938 2938
Size: 4571
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 324 874 937 324
Size: 550 63 3958
Fragments arranged by size:
3958 550 63
Bpu10I CC'TnA_GC
Cuts at: 509 1042 509
Size: 533 4038
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 652 1747 2626 3118 3416 652
Size: 1095 879 492 298 1807
Fragments arranged by size:
1807 1095 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 490 4275 490
Size: 3785 786
BsaAI yAC'GTr
Cuts at: 1924 1924
Size: 4571
BsaBI GATnn'nnATC
Cuts at: 6 2085 4122 6
Size: 2079 2037 455
Fragments arranged by size:
2079 2037 455
BsaHI Gr'CG_yC
Cuts at: 1076 1373 2002 2250 2952 4448 1076
Size: 297 629 248 702 1496 1199
Fragments arranged by size:
1496 1199 702 629 297 248
BsaWI w'CCGG_w
Cuts at: 1760 2281 2959 3233 3380 3791 1760
Size: 521 678 274 147 411 2540
Fragments arranged by size:
2540 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 128 158 778 808 1312 1342 128
Size: 30 620 30 504 30 3357
Fragments arranged by size:
3357 620 504 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 616 832 1027 1320 616
Size: 216 195 293 3867
Fragments arranged by size:
3867 293 216 195
BseYI C'CCAG_C
Cuts at: 1129 1453 1996 2754 3331 3827 1129
Size: 324 543 758 577 496 1873
Fragments arranged by size:
1873 758 577 543 496 324
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 240 379 240
Size: 139 4432
BsiEI CG_ry'CG
Cuts at: 104 2113 2159 3367 104
Size: 2009 46 1208 1308
Fragments arranged by size:
2009 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1585 2363 2553 3345 1585
Size: 778 190 792 2811
Fragments arranged by size:
2811 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 297 329 763 1407 1585 2196 2289 2363 2553
Size: 32 434 644 178 611 93 74 190
Cuts at: 2553 2615 3345 297
Size: 62 730 1523
Fragments arranged by size:
1523 730 644 611 434 190 178 93
74 62 32
BspEI T'CCGG_A
Cuts at: 3791 3791
Size: 4571
BspHI T'CATG_A
Cuts at: 230 3747 230
Size: 3517 1054
BspMI ACCTGCnnnn'nnnn_
Cuts at: 780 1621 1835 2137 2518 780
Size: 841 214 302 381 2833
Fragments arranged by size:
2833 841 381 302 214
BsrBI CCG'CTC
Cuts at: 50 249 2863 2917 4401 4566 50
Size: 199 2614 54 1484 165 55
Fragments arranged by size:
2614 1484 199 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2483 2483
Size: 4571
BsrFI r'CCGG_y
Cuts at: 2569 2750 2959 2569
Size: 181 209 4181
Fragments arranged by size:
4181 209 181
BssHII G'CGCG_C
Cuts at: 253 253
Size: 4571
BssSI C'ACGA_G
Cuts at: 313 620 2842 3200 313
Size: 307 2222 358 1684
Fragments arranged by size:
2222 1684 358 307
BstAPI GCAn_nnn'nTGC
Cuts at: 940 1948 940
Size: 1008 3563
BstBI TT'CG_AA
Cuts at: 2932 2932
Size: 4571
BstYI r'GATC_y
Cuts at: 1 1596 2063 2086 2421 2667 3679 3765 3777
Size: 1595 467 23 335 246 1012 86 12
Cuts at: 3777 4123 1
Size: 346 449
Fragments arranged by size:
1595 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 955 955
Size: 4571
BtgI C'CryG_G
Cuts at: 341 1334 341
Size: 993 3578
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2521 2702 2865 2521
Size: 181 163 4227
Fragments arranged by size:
4227 181 163
BtsI GCAGTG_nn'
Cuts at: 808 950 1814 808
Size: 142 864 3565
Fragments arranged by size:
3565 864 142
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1532 1567 1532
Size: 35 4536
DraI TTT'AAA
Cuts at: 1570 1570
Size: 4571
DraIII CAC_nnn'GTG
Cuts at: 290 290
Size: 4571
DrdI GACnn_nn'nnGTC
Cuts at: 2277 3135 2277
Size: 858 3713
EaeI y'GGCC_r
Cuts at: 207 989 1000 2156 2330 2721 2748 4093 207
Size: 782 11 1156 174 391 27 1345 685
Fragments arranged by size:
1345 1156 782 685 391 174 27 11
EagI C'GGCC_G
Cuts at: 2156 2156
Size: 4571
EarI CTCTTCn'nnn_
Cuts at: 2594 2804 2594
Size: 210 4361
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2779 3089 3235 4381 2779
Size: 310 146 1146 2969
Fragments arranged by size:
2969 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1583 1583
Size: 4571
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 324 763 874 937 2396 2828 3575 3843 324
Size: 439 111 63 1459 432 747 268 1052
Fragments arranged by size:
1459 1052 747 439 432 268 111 63
EcoO109I rG'GnC_Cy
Cuts at: 325 326 951 1404 1710 325
Size: 1 625 453 306 3186
Fragments arranged by size:
3186 625 453 306 1
EcoRI G'AATT_C
Cuts at: 1575 1575
Size: 4571
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 480 727 1527 2590 480
Size: 247 800 1063 2461
Fragments arranged by size:
2461 1063 800 247
FspI TGC'GCA
Cuts at: 275 1172 2352 275
Size: 897 1180 2494
Fragments arranged by size:
2494 1180 897
HaeII r_GCGC'y
Cuts at: 303 2005 2253 3275 4254 303
Size: 1702 248 1022 979 620
Fragments arranged by size:
1702 1022 979 620 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1003 1035 1297 1329 1363 1395 2413 2443 2445
Size: 32 262 32 34 32 1018 30 2
Cuts at: 2445 2475 1003
Size: 30 3099
Fragments arranged by size:
3099 1018 262 34 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1610 1610
Size: 4571
KasI G'GCGC_C
Cuts at: 2001 2249 2001
Size: 248 4323
KpnI G_GTAC'C
Cuts at: 1591 1591
Size: 4571
MluI A'CGCG_T
Cuts at: 4004 4004
Size: 4571
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 114 3242 3426 114
Size: 3128 184 1259
Fragments arranged by size:
3128 1259 184
MscI TGG'CCA
Cuts at: 209 991 2332 209
Size: 782 1341 2448
Fragments arranged by size:
2448 1341 782
MslI CAynn'nnrTG
Cuts at: 991 2560 2687 991
Size: 1569 127 2875
Fragments arranged by size:
2875 1569 127
MspA1I CmG'CkG
Cuts at: 937 1682 1996 2356 3369 3614 937
Size: 745 314 360 1013 245 1894
Fragments arranged by size:
1894 1013 745 360 314 245
NaeI GCC'GGC
Cuts at: 2752 2752
Size: 4571
NarI GG'CG_CC
Cuts at: 2002 2250 2002
Size: 248 4323
NdeI CA'TA_TG
Cuts at: 120 120
Size: 4571
NgoMIV G'CCGG_C
Cuts at: 2750 2750
Size: 4571
NspI r_CATG'y
Cuts at: 1624 2124 3031 1624
Size: 500 907 3164
Fragments arranged by size:
3164 907 500
PasI CC'CwG_GG
Cuts at: 539 539
Size: 4571
PciI A'CATG_T
Cuts at: 3027 3027
Size: 4571
PflMI CCAn_nnn'nTGG
Cuts at: 774 915 998 1298 774
Size: 141 83 300 4047
Fragments arranged by size:
4047 300 141 83
PfoI T'CCnGG_A
Cuts at: 839 839
Size: 4571
PmeI GTTT'AAAC
Cuts at: 1570 1570
Size: 4571
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3710 3742 3710
Size: 32 4539
PpuMI rG'GwC_Cy
Cuts at: 951 951
Size: 4571
PspOMI G'GGCC_C
Cuts at: 293 325 1403 293
Size: 32 1078 3461
Fragments arranged by size:
3461 1078 32
PspXI vC'TCGA_Gb
Cuts at: 400 400
Size: 4571
PstI C_TGCA'G
Cuts at: 1618 2303 1618
Size: 685 3886
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4571
PvuII CAG'CTG
Cuts at: 937 1996 2356 937
Size: 1059 360 3152
Fragments arranged by size:
3152 1059 360
RsrII CG'GwC_CG
Cuts at: 2766 2766
Size: 4571
SacI G_AGCT'C
Cuts at: 1585 1585
Size: 4571
SalI G'TCGA_C
Cuts at: 1608 1608
Size: 4571
SapI GCTCTTCn'nnn_
Cuts at: 2594 2804 2594
Size: 210 4361
SbfI CC_TGCA'GG
Cuts at: 1618 1618
Size: 4571
ScaI AGT'ACT
Cuts at: 4088 4088
Size: 4571
SfcI C'TryA_G
Cuts at: 33 1614 2299 3292 3483 33
Size: 1581 685 993 191 1121
Fragments arranged by size:
1581 1121 993 685 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 358 358
Size: 4571
SfoI GGC'GCC
Cuts at: 2003 2251 2003
Size: 248 4323
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4571
SmaI CCC'GGG
Cuts at: 1593 1593
Size: 4571
SmlI C'TyrA_G
Cuts at: 400 667 712 1726 2641 3133 3395 400
Size: 267 45 1014 915 492 262 1576
Fragments arranged by size:
1576 1014 915 492 267 262 45
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4571
SphI G_CATG'C
Cuts at: 1624 2124 1624
Size: 500 4071
StuI AGG'CCT
Cuts at: 352 412 352
Size: 60 4511
StyI C'CwwG_G
Cuts at: 500 1705 2059 500
Size: 1205 354 3012
Fragments arranged by size:
3012 1205 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2154 2287 2746 2955 2154
Size: 133 459 209 3770
Fragments arranged by size:
3770 459 209 133
TatI w'GTAC_w
Cuts at: 770 4086 770
Size: 3316 1255
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 128 160 943 975 4088 4120 128
Size: 32 783 32 3113 32 579
Fragments arranged by size:
3113 783 579 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1075 2368 1075
Size: 1293 3278
XbaI T'CTAG_A
Cuts at: 1602 1602
Size: 4571
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2066 2066
Size: 4571
XhoI C'TCGA_G
Cuts at: 400 400
Size: 4571
XmaI C'CCGG_G
Cuts at: 1591 1591
Size: 4571
XmnI GAAnn'nnTTC
Cuts at: 464 1360 464
Size: 896 3675
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstYI
Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NdeI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PspOMI PspXI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AleI AlfI AloI AscI AvrII BaeI
BcgI BfrBI BmgBI BmtI BsiWI BsmI BsmBI BsrGI
BstEII BstXI BstZ17I ClaI EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NcoI NheI NotI NruI NsiI
PacI PmlI PshAI PsiI PsrI SacII SanDI SexAI
SgrAI SnaBI SrfI SspI SwaI ZraI