Restriction map for
pF1KB8654
(Circular) (Six-base) MAPSORT of: pF1KB8654.seq Check: 2562 from: 1 to: 5699
pF1KB8654 5699 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 405 1438 2425 405
Size: 1033 987 3679
Fragments arranged by size:
3679 1033 987
AatII G_ACGT'C
Cuts at: 274 274
Size: 5699
AccI GT'mk_AC
Cuts at: 1898 2737 1898
Size: 839 4860
Acc65I G'GTAC_C
Cuts at: 2715 2715
Size: 5699
AclI AA'CG_TT
Cuts at: 5539 5539
Size: 5699
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1177 1389 2566 3524 3956 4309 4971 1177
Size: 212 1177 958 432 353 662 1905
Fragments arranged by size:
1905 1177 958 662 432 353 212
AflIII A'CryG_T
Cuts at: 1319 1868 3051 4851 5132 1319
Size: 549 1183 1800 281 1886
Fragments arranged by size:
1886 1800 1183 549 281
AhdI GACnn_n'nnGTC
Cuts at: 2667 2667
Size: 5699
AleI CACnn'nnGTG
Cuts at: 2579 2579
Size: 5699
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1402 1669 1402
Size: 267 5432
AlwNI CAG_nnn'CTG
Cuts at: 431 657 1851 2426 2535 3004 4442 431
Size: 226 1194 575 109 469 1438 1688
Fragments arranged by size:
1688 1438 1194 575 469 226 109
ApaI G_GGCC'C
Cuts at: 1418 2332 1418
Size: 914 4785
ApaLI G'TGCA_C
Cuts at: 1741 4537 1741
Size: 2796 2903
ApoI r'AATT_y
Cuts at: 2703 5052 5308 2703
Size: 2349 256 3094
Fragments arranged by size:
3094 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5699
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5699
AvaI C'yCGr_G
Cuts at: 1417 2147 2325 2478 2719 1417
Size: 730 178 153 241 4397
Fragments arranged by size:
4397 730 241 178 153
AvrII C'CTAG_G
Cuts at: 606 606
Size: 5699
BamHI G'GATC_C
Cuts at: 2724 2724
Size: 5699
BanI G'GyrC_C
Cuts at: 1703 2074 2715 3129 3377 3412 1703
Size: 371 641 414 248 35 3990
Fragments arranged by size:
3990 641 414 371 248 35
BanII G_rGCy'C
Cuts at: 1165 1418 1424 1604 2099 2332 2713 3743 1165
Size: 253 6 180 495 233 381 1030 3121
Fragments arranged by size:
3121 1030 495 381 253 233 180 6
BbeI G_GCGC'C
Cuts at: 1707 3133 3381 1707
Size: 1426 248 4025
Fragments arranged by size:
4025 1426 248
BbsI GAAGACnn'nnnn_
Cuts at: 118 547 1447 2517 118
Size: 429 900 1070 3300
Fragments arranged by size:
3300 1070 900 429
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1888 1922 1888
Size: 34 5665
BciVI GTATCCnnnnn_n'
Cuts at: 3597 4653 5699 3597
Size: 1056 1046 3597
Fragments arranged by size:
3597 1056 1046
BclI T'GATC_A
Cuts at: 3219 3219
Size: 5699
BglI GCCn_nnn'nGGC
Cuts at: 1300 1300
Size: 5699
BglII A'GATC_T
Cuts at: 865 3214 5251 865
Size: 2349 2037 1313
Fragments arranged by size:
2349 2037 1313
BlpI GC'TnA_GC
Cuts at: 1082 2260 2811 1082
Size: 1178 551 3970
Fragments arranged by size:
3970 1178 551
Bme1580I G_kGCm'C
Cuts at: 1418 1493 1745 2173 2332 3324 3417 4541 1418
Size: 75 252 428 159 992 93 1124 2576
Fragments arranged by size:
2576 1124 992 428 252 159 93 75
BmgBI CAC'GTC
Cuts at: 1869 1869
Size: 5699
BmrI ACTGGGnnnn_n'
Cuts at: 242 837 891 1203 2911 2992 3327 5471 242
Size: 595 54 312 1708 81 335 2144 470
Fragments arranged by size:
2144 1708 595 470 335 312 81 54
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 651 4066 651
Size: 3415 2284
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 964 1516 1579 964
Size: 552 63 5084
Fragments arranged by size:
5084 552 63
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1696 2875 3754 4468 4766 1696
Size: 1179 879 714 298 2629
Fragments arranged by size:
2629 1179 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 814 1120 2044 5403 814
Size: 306 924 3359 1110
Fragments arranged by size:
3359 1110 924 306
BsaAI yAC'GTr
Cuts at: 288 3052 288
Size: 2764 2935
BsaBI GATnn'nnATC
Cuts at: 6 3213 5250 6
Size: 3207 2037 455
Fragments arranged by size:
3207 2037 455
BsaHI Gr'CG_yC
Cuts at: 271 1256 1704 3130 3378 4080 5576 271
Size: 985 448 1426 248 702 1496 394
Fragments arranged by size:
1496 1426 985 702 448 394 248
BsaWI w'CCGG_w
Cuts at: 2888 3409 4087 4498 4645 4919 2888
Size: 521 678 411 147 274 3668
Fragments arranged by size:
3668 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1513 1513
Size: 5699
BseYI C'CCAG_C
Cuts at: 2268 3124 3882 4547 4955 2268
Size: 856 758 665 408 3012
Fragments arranged by size:
3012 856 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1814 2014 1814
Size: 200 5499
BsiEI CG_ry'CG
Cuts at: 104 3241 3287 4517 104
Size: 3137 46 1230 1286
Fragments arranged by size:
3137 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 122 1396 1424 1604 1745 1778 2099 2713 3491
Size: 1274 28 180 141 33 321 614 778
Cuts at: 3491 3681 4541 122
Size: 190 860 1280
Fragments arranged by size:
1280 1274 860 778 614 321 190 180
141 33 28
BsmI GAATG_Cn'
Cuts at: 679 679
Size: 5699
BsmBI CGTCTCn'nnnn_
Cuts at: 2482 2482
Size: 5699
Bsp1286I G_dGCh'C
Cuts at: 122 1165 1396 1418 1424 1493 1604 1745 1778
Size: 1043 231 22 6 69 111 141 33
Cuts at: 1778 2099 2173 2332 2713 3324 3417 3491 3681
Size: 321 74 159 381 611 93 74 190
Cuts at: 3681 3743 4541 122
Size: 62 798 1280
Fragments arranged by size:
1280 1043 798 611 381 321 231 190
159 141 111 93 74 74 69 62
33 22 6
BspHI T'CATG_A
Cuts at: 515 1997 4131 515
Size: 1482 2134 2083
Fragments arranged by size:
2134 2083 1482
BspMI ACCTGCnnnn'nnnn_
Cuts at: 405 671 1394 1438 2425 2749 2963 3265 3646
Size: 266 723 44 987 324 214 302 381
Cuts at: 3646 405
Size: 2458
Fragments arranged by size:
2458 987 723 381 324 302 266 214
44
BsrBI CCG'CTC
Cuts at: 50 1086 1700 2300 3991 4045 5529 5694 50
Size: 1036 614 600 1691 54 1484 165 55
Fragments arranged by size:
1691 1484 1036 614 600 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1904 3611 1904
Size: 1707 3992
BsrFI r'CCGG_y
Cuts at: 1258 1706 3697 3878 1258
Size: 448 1991 181 3079
Fragments arranged by size:
3079 1991 448 181
BssSI C'ACGA_G
Cuts at: 223 3970 4678 223
Size: 3747 708 1244
Fragments arranged by size:
3747 1244 708
BstAPI GCAn_nnn'nTGC
Cuts at: 431 1071 1488 2222 2357 3076 431
Size: 640 417 734 135 719 3054
Fragments arranged by size:
3054 734 719 640 417 135
BstBI TT'CG_AA
Cuts at: 4060 4060
Size: 5699
BstYI r'GATC_y
Cuts at: 1 865 2724 3191 3214 3549 3795 4101 4113
Size: 864 1859 467 23 335 246 306 12
Cuts at: 4113 4199 5251 1
Size: 86 1052 449
Fragments arranged by size:
1859 1052 864 467 449 335 306 246
86 23 12
BtgI C'CryG_G
Cuts at: 365 528 596 1117 1240 1361 2577 365
Size: 163 68 521 123 121 1216 3487
Fragments arranged by size:
3487 1216 521 163 123 121 68
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1341 2060 2498 3649 3830 3993 1341
Size: 719 438 1151 181 163 3047
Fragments arranged by size:
3047 1151 719 438 181 163
BtsI GCAGTG_nn'
Cuts at: 1797 1802 2031 2040 2373 2427 2942 1797
Size: 5 229 9 333 54 515 4554
Fragments arranged by size:
4554 515 333 229 54 9 5
DraI TTT'AAA
Cuts at: 929 2698 929
Size: 1769 3930
DraIII CAC_nnn'GTG
Cuts at: 1978 2397 1978
Size: 419 5280
DrdI GACnn_nn'nnGTC
Cuts at: 3405 4749 3405
Size: 1344 4355
EaeI y'GGCC_r
Cuts at: 246 1034 1952 2277 2678 3284 3458 3849 3876
Size: 788 918 325 401 606 174 391 27
Cuts at: 3876 5221 246
Size: 1345 724
Fragments arranged by size:
1345 918 788 724 606 401 391 325
174 27
EagI C'GGCC_G
Cuts at: 3284 3284
Size: 5699
EarI CTCTTCn'nnn_
Cuts at: 1047 1429 3722 3932 1047
Size: 382 2293 210 2814
Fragments arranged by size:
2814 2293 382 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2397 3907 4649 4795 5509 2397
Size: 1510 742 146 714 2587
Fragments arranged by size:
2587 1510 742 714 146
EcoICRI GAG'CTC
Cuts at: 1422 1602 2097 2711 1422
Size: 180 495 614 4410
Fragments arranged by size:
4410 614 495 180
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 964 1177 1389 1516 1579 2566 3524 3956 4309
Size: 213 212 127 63 987 958 432 353
Cuts at: 4309 4971 964
Size: 662 1692
Fragments arranged by size:
1692 987 958 662 432 353 213 212
127 63
EcoNI CCTnn'n_nnAGG
Cuts at: 1591 1591
Size: 5699
EcoO109I rG'GnC_Cy
Cuts at: 209 236 998 1848 2329 2627 2838 209
Size: 27 762 850 481 298 211 3070
Fragments arranged by size:
3070 850 762 481 298 211 27
EcoRI G'AATT_C
Cuts at: 2703 2703
Size: 5699
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 159 3718 159
Size: 3559 2140
FspI TGC'GCA
Cuts at: 3480 3480
Size: 5699
HaeII r_GCGC'y
Cuts at: 634 1707 3133 3381 4611 5382 634
Size: 1073 1426 248 1230 771 951
Fragments arranged by size:
1426 1230 1073 951 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1340 1372 2308 2340 2653 2685 3541 3571 3573
Size: 32 936 32 313 32 856 30 2
Cuts at: 3573 3603 1340
Size: 30 3436
Fragments arranged by size:
3436 936 856 313 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 849 1899 2738 849
Size: 1050 839 3810
Fragments arranged by size:
3810 1050 839
HpaI GTT'AAC
Cuts at: 849 849
Size: 5699
KasI G'GCGC_C
Cuts at: 1703 3129 3377 1703
Size: 1426 248 4025
Fragments arranged by size:
4025 1426 248
KpnI G_GTAC'C
Cuts at: 2719 2719
Size: 5699
MfeI C'AATT_G
Cuts at: 155 155
Size: 5699
MluI A'CGCG_T
Cuts at: 1319 5132 1319
Size: 3813 1886
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 790 4458 4642 790
Size: 3668 184 1847
Fragments arranged by size:
3668 1847 184
MscI TGG'CCA
Cuts at: 248 1036 3460 248
Size: 788 2424 2487
Fragments arranged by size:
2487 2424 788
MslI CAynn'nnrTG
Cuts at: 1282 1612 1651 2045 2579 3688 3815 1282
Size: 330 39 394 534 1109 127 3166
Fragments arranged by size:
3166 1109 534 394 330 127 39
MspA1I CmG'CkG
Cuts at: 1242 1521 1809 2102 2166 2579 2810 3124 3484
Size: 279 288 293 64 413 231 314 360
Cuts at: 3484 4268 4513 1242
Size: 784 245 2428
Fragments arranged by size:
2428 784 413 360 314 293 288 279
245 231 64
NaeI GCC'GGC
Cuts at: 1260 1708 3880 1260
Size: 448 2172 3079
Fragments arranged by size:
3079 2172 448
NarI GG'CG_CC
Cuts at: 1704 3130 3378 1704
Size: 1426 248 4025
Fragments arranged by size:
4025 1426 248
NcoI C'CATG_G
Cuts at: 528 596 528
Size: 68 5631
NgoMIV G'CCGG_C
Cuts at: 1258 1706 3878 1258
Size: 448 2172 3079
Fragments arranged by size:
3079 2172 448
NspI r_CATG'y
Cuts at: 2268 2554 2752 3252 4855 2268
Size: 286 198 500 1603 3112
Fragments arranged by size:
3112 1603 500 286 198
PasI CC'CwG_GG
Cuts at: 771 1824 2632 771
Size: 1053 808 3838
Fragments arranged by size:
3838 1053 808
PciI A'CATG_T
Cuts at: 4851 4851
Size: 5699
PflMI CCAn_nnn'nTGG
Cuts at: 657 1851 1863 2286 2535 657
Size: 1194 12 423 249 3821
Fragments arranged by size:
3821 1194 423 249 12
PfoI T'CCnGG_A
Cuts at: 1435 2528 1435
Size: 1093 4606
PmeI GTTT'AAAC
Cuts at: 2698 2698
Size: 5699
PmlI CAC'GTG
Cuts at: 288 288
Size: 5699
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4145 4177 4145
Size: 32 5667
PpuMI rG'GwC_Cy
Cuts at: 236 1848 236
Size: 1612 4087
PshAI GACnn'nnGTC
Cuts at: 274 274
Size: 5699
PspOMI G'GGCC_C
Cuts at: 1414 2328 1414
Size: 914 4785
PstI C_TGCA'G
Cuts at: 416 839 1391 2422 2746 3431 416
Size: 423 552 1031 324 685 2684
Fragments arranged by size:
2684 1031 685 552 423 324
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5699
PvuII CAG'CTG
Cuts at: 1809 3124 3484 1809
Size: 1315 360 4024
Fragments arranged by size:
4024 1315 360
RsrII CG'GwC_CG
Cuts at: 3894 3894
Size: 5699
SacI G_AGCT'C
Cuts at: 1424 1604 2099 2713 1424
Size: 180 495 614 4410
Fragments arranged by size:
4410 614 495 180
SacII CC_GC'GG
Cuts at: 1243 2580 1243
Size: 1337 4362
SalI G'TCGA_C
Cuts at: 1897 2736 1897
Size: 839 4860
SapI GCTCTTCn'nnn_
Cuts at: 1047 1429 3722 3932 1047
Size: 382 2293 210 2814
Fragments arranged by size:
2814 2293 382 210
SbfI CC_TGCA'GG
Cuts at: 416 2746 416
Size: 2330 3369
ScaI AGT'ACT
Cuts at: 5216 5216
Size: 5699
SexAI A'CCwGG_T
Cuts at: 685 685
Size: 5699
SfcI C'TryA_G
Cuts at: 33 412 835 1387 2418 2742 3427 4395 4586
Size: 379 423 552 1031 324 685 968 191
Cuts at: 4586 33
Size: 1146
Fragments arranged by size:
1146 1031 968 685 552 423 379 324
191
SfoI GGC'GCC
Cuts at: 1705 3131 3379 1705
Size: 1426 248 4025
Fragments arranged by size:
4025 1426 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5699
SgrAI Cr'CCGG_yG
Cuts at: 1258 1258
Size: 5699
SmaI CCC'GGG
Cuts at: 2480 2721 2480
Size: 241 5458
SmlI C'TyrA_G
Cuts at: 1711 2854 3769 4483 4745 1711
Size: 1143 915 714 262 2665
Fragments arranged by size:
2665 1143 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5699
SphI G_CATG'C
Cuts at: 2268 2554 2752 3252 2268
Size: 286 198 500 4715
Fragments arranged by size:
4715 500 286 198
SspI AAT'ATT
Cuts at: 703 703
Size: 5699
StyI C'CwwG_G
Cuts at: 143 528 596 606 842 2657 2833 3187 143
Size: 385 68 10 236 1815 176 354 2655
Fragments arranged by size:
2655 1815 385 354 236 176 68 10
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 819 902 1147 2204 3282 3415 3874 4083 819
Size: 83 245 1057 1078 133 459 209 2435
Fragments arranged by size:
2435 1078 1057 459 245 209 133 83
TatI w'GTAC_w
Cuts at: 227 1337 2003 5214 227
Size: 1110 666 3211 712
Fragments arranged by size:
3211 1110 712 666
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 705 737 767 799 5216 5248 705
Size: 32 30 32 4417 32 1156
Fragments arranged by size:
4417 1156 32 32 32 30
Tth111I GACn'n_nGTC
Cuts at: 2521 3496 2521
Size: 975 4724
XbaI T'CTAG_A
Cuts at: 172 2730 172
Size: 2558 3141
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 229 396 824 1044 1911 2340 3194 229
Size: 167 428 220 867 429 854 2734
Fragments arranged by size:
2734 867 854 429 428 220 167
XmaI C'CCGG_G
Cuts at: 2478 2719 2478
Size: 241 5458
ZraI GAC'GTC
Cuts at: 272 272
Size: 5699
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AhdI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PspOMI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI ZraI
Enzymes that do not cut:
AfeI AflII AgeI AloI AscI BaeI BbvCI BfrBI
BmtI Bpu10I BsaXI BsiWI BspEI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI EcoRV FseI FspAI
HindIII NdeI NheI NotI NruI NsiI PacI PsiI
PspXI PsrI SanDI SfiI SnaBI SrfI StuI SwaI
XhoI XmnI