Restriction map for
pF1KB8656
(Circular) (Six-base) MAPSORT of: pF1KB8656.seq Check: 5428 from: 1 to: 4814
pF1KB8656 4814 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 801 1250 801
Size: 449 4365
AccI GT'mk_AC
Cuts at: 209 492 1262 1852 209
Size: 283 770 590 3171
Fragments arranged by size:
3171 770 590 283
Acc65I G'GTAC_C
Cuts at: 1830 1830
Size: 4814
AclI AA'CG_TT
Cuts at: 4654 4654
Size: 4814
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 455 1006 1081 2639 3071 3424 4086 455
Size: 551 75 1558 432 353 662 1183
Fragments arranged by size:
1558 1183 662 551 432 353 75
AflIII A'CryG_T
Cuts at: 413 2166 3966 4247 413
Size: 1753 1800 281 980
Fragments arranged by size:
1800 1753 980 281
AgeI A'CCGG_T
Cuts at: 644 644
Size: 4814
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 228 228
Size: 4814
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 526 558 526
Size: 32 4782
AlwNI CAG_nnn'CTG
Cuts at: 2119 3557 2119
Size: 1438 3376
ApaI G_GGCC'C
Cuts at: 581 581
Size: 4814
ApaLI G'TGCA_C
Cuts at: 3652 3652
Size: 4814
ApoI r'AATT_y
Cuts at: 636 1142 1347 1480 1818 4167 4423 636
Size: 506 205 133 338 2349 256 1027
Fragments arranged by size:
2349 1027 506 338 256 205 133
AseI AT'TA_AT
Cuts at: 20 20
Size: 4814
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4814
AvaI C'yCGr_G
Cuts at: 1834 1834
Size: 4814
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1093 1126 1093
Size: 33 4781
BamHI G'GATC_C
Cuts at: 1839 1839
Size: 4814
BanI G'GyrC_C
Cuts at: 1440 1830 2244 2492 2527 1440
Size: 390 414 248 35 3727
Fragments arranged by size:
3727 414 390 248 35
BanII G_rGCy'C
Cuts at: 386 581 595 603 1828 2858 386
Size: 195 14 8 1225 1030 2342
Fragments arranged by size:
2342 1225 1030 195 14 8
BbeI G_GCGC'C
Cuts at: 2248 2496 2248
Size: 248 4566
BciVI GTATCCnnnnn_n'
Cuts at: 2712 3768 4814 2712
Size: 1056 1046 2712
Fragments arranged by size:
2712 1056 1046
BclI T'GATC_A
Cuts at: 2334 2334
Size: 4814
BglII A'GATC_T
Cuts at: 2329 4366 2329
Size: 2037 2777
BlpI GC'TnA_GC
Cuts at: 1926 1926
Size: 4814
Bme1580I G_kGCm'C
Cuts at: 581 2439 2532 3656 581
Size: 1858 93 1124 1739
Fragments arranged by size:
1858 1739 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 668 2026 2107 2442 4586 668
Size: 1358 81 335 2144 896
Fragments arranged by size:
2144 1358 896 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 292 552 1305 3181 292
Size: 260 753 1876 1925
Fragments arranged by size:
1925 1876 753 260
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 522 617 1704 522
Size: 95 1087 3632
Fragments arranged by size:
3632 1087 95
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 204 1990 2869 3583 3881 204
Size: 1786 879 714 298 1137
Fragments arranged by size:
1786 1137 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4518 4518
Size: 4814
BsaAI yAC'GTr
Cuts at: 2167 2167
Size: 4814
BsaBI GATnn'nnATC
Cuts at: 6 441 1577 2328 4365 6
Size: 435 1136 751 2037 455
Fragments arranged by size:
2037 1136 751 455 435
BsaHI Gr'CG_yC
Cuts at: 2245 2493 3195 4691 2245
Size: 248 702 1496 2368
Fragments arranged by size:
2368 1496 702 248
BsaWI w'CCGG_w
Cuts at: 644 2003 2524 3202 3613 3760 4034 644
Size: 1359 521 678 411 147 274 1424
Fragments arranged by size:
1424 1359 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 725 725
Size: 4814
BseYI C'CCAG_C
Cuts at: 1342 2239 2997 3662 4070 1342
Size: 897 758 665 408 2086
Fragments arranged by size:
2086 897 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1257 1257
Size: 4814
BsiEI CG_ry'CG
Cuts at: 104 2356 2402 3632 104
Size: 2252 46 1230 1286
Fragments arranged by size:
2252 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 190 595 1828 2606 2796 3656 190
Size: 405 1233 778 190 860 1348
Fragments arranged by size:
1348 1233 860 778 405 190
BsmI GAATG_Cn'
Cuts at: 1026 1509 1026
Size: 483 4331
Bsp1286I G_dGCh'C
Cuts at: 190 386 581 595 603 1828 2439 2532 2606
Size: 196 195 14 8 1225 611 93 74
Cuts at: 2606 2796 2858 3656 190
Size: 190 62 798 1348
Fragments arranged by size:
1348 1225 798 611 196 195 190 93
74 62 14 8
BspHI T'CATG_A
Cuts at: 1743 3246 1743
Size: 1503 3311
BspMI ACCTGCnnnn'nnnn_
Cuts at: 801 1250 1864 2078 2380 2761 801
Size: 449 614 214 302 381 2854
Fragments arranged by size:
2854 614 449 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3106 3160 4644 4809 50
Size: 3056 54 1484 165 55
Fragments arranged by size:
3056 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 335 1049 2726 335
Size: 714 1677 2423
Fragments arranged by size:
2423 1677 714
BsrFI r'CCGG_y
Cuts at: 644 2812 2993 644
Size: 2168 181 2465
Fragments arranged by size:
2465 2168 181
BssSI C'ACGA_G
Cuts at: 3085 3793 3085
Size: 708 4106
BstAPI GCAn_nnn'nTGC
Cuts at: 2191 2191
Size: 4814
BstBI TT'CG_AA
Cuts at: 664 3175 664
Size: 2511 2303
BstYI r'GATC_y
Cuts at: 1 681 1839 2306 2329 2664 2910 3216 3228
Size: 680 1158 467 23 335 246 306 12
Cuts at: 3228 3314 4366 1
Size: 86 1052 449
Fragments arranged by size:
1158 1052 680 467 449 335 306 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 387 387
Size: 4814
BtgI C'CryG_G
Cuts at: 107 107
Size: 4814
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2764 2945 3108 2764
Size: 181 163 4470
Fragments arranged by size:
4470 181 163
BtsI GCAGTG_nn'
Cuts at: 2057 2057
Size: 4814
DraI TTT'AAA
Cuts at: 174 746 861 1813 174
Size: 572 115 952 3175
Fragments arranged by size:
3175 952 572 115
DraIII CAC_nnn'GTG
Cuts at: 298 676 298
Size: 378 4436
DrdI GACnn_nn'nnGTC
Cuts at: 2520 3864 2520
Size: 1344 3470
EaeI y'GGCC_r
Cuts at: 2399 2573 2964 2991 4336 2399
Size: 174 391 27 1345 2877
Fragments arranged by size:
2877 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2399 2399
Size: 4814
EarI CTCTTCn'nnn_
Cuts at: 261 474 1025 2837 3047 261
Size: 213 551 1812 210 2028
Fragments arranged by size:
2028 1812 551 213 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3022 3764 3910 4624 3022
Size: 742 146 714 3212
Fragments arranged by size:
3212 742 714 146
EcoICRI GAG'CTC
Cuts at: 593 1826 593
Size: 1233 3581
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 455 522 617 1006 1081 1704 2639 3071 3424
Size: 67 95 389 75 623 935 432 353
Cuts at: 3424 4086 455
Size: 662 1183
Fragments arranged by size:
1183 935 662 623 432 389 353 95
75 67
EcoNI CCTnn'n_nnAGG
Cuts at: 450 977 1035 450
Size: 527 58 4229
Fragments arranged by size:
4229 527 58
EcoO109I rG'GnC_Cy
Cuts at: 578 1953 578
Size: 1375 3439
EcoRI G'AATT_C
Cuts at: 1347 1818 1347
Size: 471 4343
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2833 2833
Size: 4814
FspI TGC'GCA
Cuts at: 2595 2595
Size: 4814
HaeII r_GCGC'y
Cuts at: 2248 2496 3726 4497 2248
Size: 248 1230 771 2565
Fragments arranged by size:
2565 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2656 2686 2688 2718 2656
Size: 30 2 30 4752
Fragments arranged by size:
4752 30 30 2
HincII GTy'rAC
Cuts at: 1405 1853 1405
Size: 448 4366
HindIII A'AGCT_T
Cuts at: 855 855
Size: 4814
KasI G'GCGC_C
Cuts at: 2244 2492 2244
Size: 248 4566
KpnI G_GTAC'C
Cuts at: 1834 1834
Size: 4814
MfeI C'AATT_G
Cuts at: 721 721
Size: 4814
MluI A'CGCG_T
Cuts at: 4247 4247
Size: 4814
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3573 3757 3573
Size: 184 4630
MscI TGG'CCA
Cuts at: 2575 2575
Size: 4814
MslI CAynn'nnrTG
Cuts at: 1777 2803 2930 1777
Size: 1026 127 3661
Fragments arranged by size:
3661 1026 127
MspA1I CmG'CkG
Cuts at: 880 1925 2239 2599 3383 3628 880
Size: 1045 314 360 784 245 2066
Fragments arranged by size:
2066 1045 784 360 314 245
NaeI GCC'GGC
Cuts at: 2995 2995
Size: 4814
NarI GG'CG_CC
Cuts at: 2245 2493 2245
Size: 248 4566
NcoI C'CATG_G
Cuts at: 107 107
Size: 4814
NdeI CA'TA_TG
Cuts at: 942 942
Size: 4814
NgoMIV G'CCGG_C
Cuts at: 2993 2993
Size: 4814
NspI r_CATG'y
Cuts at: 417 1252 1867 2367 3970 417
Size: 835 615 500 1603 1261
Fragments arranged by size:
1603 1261 835 615 500
PciI A'CATG_T
Cuts at: 413 3966 413
Size: 3553 1261
PmeI GTTT'AAAC
Cuts at: 1813 1813
Size: 4814
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3260 3292 3260
Size: 32 4782
PsiI TTA'TAA
Cuts at: 344 1499 1525 344
Size: 1155 26 3633
Fragments arranged by size:
3633 1155 26
PspOMI G'GGCC_C
Cuts at: 577 577
Size: 4814
PstI C_TGCA'G
Cuts at: 207 1861 2546 207
Size: 1654 685 2475
Fragments arranged by size:
2475 1654 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4814
PvuII CAG'CTG
Cuts at: 880 2239 2599 880
Size: 1359 360 3095
Fragments arranged by size:
3095 1359 360
RsrII CG'GwC_CG
Cuts at: 3009 3009
Size: 4814
SacI G_AGCT'C
Cuts at: 595 1828 595
Size: 1233 3581
SalI G'TCGA_C
Cuts at: 1851 1851
Size: 4814
SapI GCTCTTCn'nnn_
Cuts at: 1025 2837 3047 1025
Size: 1812 210 2792
Fragments arranged by size:
2792 1812 210
SbfI CC_TGCA'GG
Cuts at: 1861 1861
Size: 4814
ScaI AGT'ACT
Cuts at: 4331 4331
Size: 4814
SfcI C'TryA_G
Cuts at: 33 203 1857 2542 3510 3701 33
Size: 170 1654 685 968 191 1146
Fragments arranged by size:
1654 1146 968 685 191 170
SfoI GGC'GCC
Cuts at: 2246 2494 2246
Size: 248 4566
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4814
SmaI CCC'GGG
Cuts at: 1836 1836
Size: 4814
SmlI C'TyrA_G
Cuts at: 183 1969 2884 3598 3860 183
Size: 1786 915 714 262 1137
Fragments arranged by size:
1786 1137 915 714 262
SpeI A'CTAG_T
Cuts at: 67 459 67
Size: 392 4422
SphI G_CATG'C
Cuts at: 1867 2367 1867
Size: 500 4314
StyI C'CwwG_G
Cuts at: 107 1036 1948 2302 107
Size: 929 912 354 2619
Fragments arranged by size:
2619 929 912 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 687 2397 2530 2989 3198 687
Size: 1710 133 459 209 2303
Fragments arranged by size:
2303 1710 459 209 133
TatI w'GTAC_w
Cuts at: 4329 4329
Size: 4814
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 507 539 4331 4363 507
Size: 32 3792 32 958
Fragments arranged by size:
3792 958 32 32
Tth111I GACn'n_nGTC
Cuts at: 2611 2611
Size: 4814
XbaI T'CTAG_A
Cuts at: 1845 1845
Size: 4814
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2309 2309
Size: 4814
XmaI C'CCGG_G
Cuts at: 1834 1834
Size: 4814
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PciI
PmeI PpiI PsiI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AscI AvrII BbsI
BbvCI BcgI BfrBI BglI BmgBI BmtI Bpu10I BsaXI
BsiWI BsmBI BspEI BsrGI BssHII BstEII BstXI BstZ17I
ClaI CspCI EcoRV FseI FspAI HpaI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmlI PpuMI
PshAI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI