Restriction map for
pF1KB8657
(Circular) (Six-base) MAPSORT of: pF1KB8657.seq Check: 6879 from: 1 to: 4475
pF1KB8657 4475 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AccI GT'mk_AC
Cuts at: 1513 1513
Size: 4475
Acc65I G'GTAC_C
Cuts at: 1163 1491 1163
Size: 328 4147
AclI AA'CG_TT
Cuts at: 4315 4315
Size: 4475
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 858 2300 2732 3085 3747 858
Size: 1442 432 353 662 1586
Fragments arranged by size:
1586 1442 662 432 353
AflIII A'CryG_T
Cuts at: 1172 1827 3627 3908 1172
Size: 655 1800 281 1739
Fragments arranged by size:
1800 1739 655 281
AleI CACnn'nnGTG
Cuts at: 184 184
Size: 4475
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 874 906 874
Size: 32 4443
AlwNI CAG_nnn'CTG
Cuts at: 492 1780 3218 492
Size: 1288 1438 1749
Fragments arranged by size:
1749 1438 1288
ApaLI G'TGCA_C
Cuts at: 922 3313 922
Size: 2391 2084
ApoI r'AATT_y
Cuts at: 268 1479 3828 4084 268
Size: 1211 2349 256 659
Fragments arranged by size:
2349 1211 659 256
AseI AT'TA_AT
Cuts at: 20 677 20
Size: 657 3818
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4475
AvaI C'yCGr_G
Cuts at: 937 1495 937
Size: 558 3917
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1319 1352 1319
Size: 33 4442
BamHI G'GATC_C
Cuts at: 1500 1500
Size: 4475
BanI G'GyrC_C
Cuts at: 139 1150 1163 1390 1491 1905 2153 2188 139
Size: 1011 13 227 101 414 248 35 2426
Fragments arranged by size:
2426 1011 414 248 227 101 35 13
BanII G_rGCy'C
Cuts at: 971 1489 2519 971
Size: 518 1030 2927
Fragments arranged by size:
2927 1030 518
BbeI G_GCGC'C
Cuts at: 143 1394 1909 2157 143
Size: 1251 515 248 2461
Fragments arranged by size:
2461 1251 515 248
BbsI GAAGACnn'nnnn_
Cuts at: 657 657
Size: 4475
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1047 1081 1360 1394 1047
Size: 34 279 34 4128
Fragments arranged by size:
4128 279 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2373 3429 4475 2373
Size: 1056 1046 2373
Fragments arranged by size:
2373 1056 1046
BclI T'GATC_A
Cuts at: 1995 1995
Size: 4475
BglII A'GATC_T
Cuts at: 305 434 1259 1990 4027 305
Size: 129 825 731 2037 753
Fragments arranged by size:
2037 825 753 731 129
BlpI GC'TnA_GC
Cuts at: 1587 1587
Size: 4475
Bme1580I G_kGCm'C
Cuts at: 926 1212 2100 2193 3317 926
Size: 286 888 93 1124 2084
Fragments arranged by size:
2084 1124 888 286 93
BmrI ACTGGGnnnn_n'
Cuts at: 1687 1768 2103 4247 1687
Size: 81 335 2144 1915
Fragments arranged by size:
2144 1915 335 81
BmtI G_CTAG'C
Cuts at: 1117 1117
Size: 4475
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2842 2842
Size: 4475
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 342 526 888 1420 342
Size: 184 362 532 3397
Fragments arranged by size:
3397 532 362 184
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1651 2530 3244 3542 1651
Size: 879 714 298 2584
Fragments arranged by size:
2584 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 910 4179 910
Size: 3269 1206
BsaAI yAC'GTr
Cuts at: 1335 1828 1335
Size: 493 3982
BsaBI GATnn'nnATC
Cuts at: 6 1989 4026 6
Size: 1983 2037 455
Fragments arranged by size:
2037 1983 455
BsaHI Gr'CG_yC
Cuts at: 140 568 1391 1906 2154 2856 4352 140
Size: 428 823 515 248 702 1496 263
Fragments arranged by size:
1496 823 702 515 428 263 248
BsaWI w'CCGG_w
Cuts at: 259 1664 2185 2863 3274 3421 3695 259
Size: 1405 521 678 411 147 274 1039
Fragments arranged by size:
1405 1039 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 436 466 502 532 436
Size: 30 36 30 4379
Fragments arranged by size:
4379 36 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 523 851 979 523
Size: 328 128 4019
Fragments arranged by size:
4019 328 128
BseYI C'CCAG_C
Cuts at: 1900 2658 3323 3731 1900
Size: 758 665 408 2644
Fragments arranged by size:
2644 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 679 679
Size: 4475
BsiEI CG_ry'CG
Cuts at: 104 2017 2063 3293 104
Size: 1913 46 1230 1286
Fragments arranged by size:
1913 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 926 1004 1445 1489 2267 2457 3317 926
Size: 78 441 44 778 190 860 2084
Fragments arranged by size:
2084 860 778 441 190 78 44
BsmI GAATG_Cn'
Cuts at: 319 319
Size: 4475
BsmBI CGTCTCn'nnnn_
Cuts at: 559 559
Size: 4475
Bsp1286I G_dGCh'C
Cuts at: 926 971 1004 1212 1445 1489 2100 2193 2267
Size: 45 33 208 233 44 611 93 74
Cuts at: 2267 2457 2519 3317 926
Size: 190 62 798 2084
Fragments arranged by size:
2084 798 611 233 208 190 93 74
62 45 44 33
BspHI T'CATG_A
Cuts at: 2907 2907
Size: 4475
BspMI ACCTGCnnnn'nnnn_
Cuts at: 651 1031 1525 1739 2041 2422 651
Size: 380 494 214 302 381 2704
Fragments arranged by size:
2704 494 381 380 302 214
BsrBI CCG'CTC
Cuts at: 50 128 2767 2821 4305 4470 50
Size: 78 2639 54 1484 165 55
Fragments arranged by size:
2639 1484 165 78 55 54
BsrDI GCAATG_nn'
Cuts at: 2387 2387
Size: 4475
BsrFI r'CCGG_y
Cuts at: 136 142 1226 2473 2654 136
Size: 6 1084 1247 181 1957
Fragments arranged by size:
1957 1247 1084 181 6
BsrGI T'GTAC_A
Cuts at: 518 1169 1175 518
Size: 651 6 3818
Fragments arranged by size:
3818 651 6
BssSI C'ACGA_G
Cuts at: 2746 3454 2746
Size: 708 3767
BstAPI GCAn_nnn'nTGC
Cuts at: 1852 1852
Size: 4475
BstBI TT'CG_AA
Cuts at: 2836 2836
Size: 4475
BstXI CCAn_nnnn'nTGG
Cuts at: 114 114
Size: 4475
BstYI r'GATC_y
Cuts at: 1 305 434 1130 1259 1500 1967 1990 2325
Size: 304 129 696 129 241 467 23 335
Cuts at: 2325 2571 2877 2889 2975 4027 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1052 696 467 449 335 306 304 246
241 129 129 86 23 12
BtgI C'CryG_G
Cuts at: 761 1070 1135 761
Size: 309 65 4101
Fragments arranged by size:
4101 309 65
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2425 2606 2769 2425
Size: 181 163 4131
Fragments arranged by size:
4131 181 163
BtsI GCAGTG_nn'
Cuts at: 1718 1718
Size: 4475
DraI TTT'AAA
Cuts at: 1474 1474
Size: 4475
DrdI GACnn_nn'nnGTC
Cuts at: 2181 3525 2181
Size: 1344 3131
EaeI y'GGCC_r
Cuts at: 144 216 371 1092 2060 2234 2625 2652 3997
Size: 72 155 721 968 174 391 27 1345
Cuts at: 3997 144
Size: 622
Fragments arranged by size:
1345 968 721 622 391 174 155 72
27
EagI C'GGCC_G
Cuts at: 2060 2060
Size: 4475
EarI CTCTTCn'nnn_
Cuts at: 160 2498 2708 160
Size: 2338 210 1927
Fragments arranged by size:
2338 1927 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 888 1066 2683 3425 3571 4285 888
Size: 178 1617 742 146 714 1078
Fragments arranged by size:
1617 1078 742 714 178 146
EcoICRI GAG'CTC
Cuts at: 1487 1487
Size: 4475
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 342 526 858 888 1420 2300 2732 3085 3747
Size: 184 332 30 532 880 432 353 662
Cuts at: 3747 342
Size: 1070
Fragments arranged by size:
1070 880 662 532 432 353 332 184
30
EcoNI CCTnn'n_nnAGG
Cuts at: 1429 1429
Size: 4475
EcoO109I rG'GnC_Cy
Cuts at: 1614 1614
Size: 4475
EcoRI G'AATT_C
Cuts at: 1479 1479
Size: 4475
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2494 2494
Size: 4475
FspI TGC'GCA
Cuts at: 2256 2256
Size: 4475
HaeII r_GCGC'y
Cuts at: 143 1394 1418 1909 2157 3387 4158 143
Size: 1251 24 491 248 1230 771 460
Fragments arranged by size:
1251 1230 771 491 460 248 24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 690 722 803 835 1258 1290 2317 2347 2349
Size: 32 81 32 423 32 1027 30 2
Cuts at: 2349 2379 690
Size: 30 2786
Fragments arranged by size:
2786 1027 423 81 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1514 1514
Size: 4475
KasI G'GCGC_C
Cuts at: 139 1390 1905 2153 139
Size: 1251 515 248 2461
Fragments arranged by size:
2461 1251 515 248
KpnI G_GTAC'C
Cuts at: 1167 1495 1167
Size: 328 4147
MluI A'CGCG_T
Cuts at: 3908 3908
Size: 4475
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 479 3234 3418 479
Size: 2755 184 1536
Fragments arranged by size:
2755 1536 184
MscI TGG'CCA
Cuts at: 373 2236 373
Size: 1863 2612
MslI CAynn'nnrTG
Cuts at: 184 544 2464 2591 184
Size: 360 1920 127 2068
Fragments arranged by size:
2068 1920 360 127
MspA1I CmG'CkG
Cuts at: 150 208 1137 1315 1586 1900 2260 3044 3289
Size: 58 929 178 271 314 360 784 245
Cuts at: 3289 150
Size: 1336
Fragments arranged by size:
1336 929 784 360 314 271 245 178
58
NaeI GCC'GGC
Cuts at: 138 144 2656 138
Size: 6 2512 1957
Fragments arranged by size:
2512 1957 6
NarI GG'CG_CC
Cuts at: 140 1391 1906 2154 140
Size: 1251 515 248 2461
Fragments arranged by size:
2461 1251 515 248
NgoMIV G'CCGG_C
Cuts at: 136 142 2654 136
Size: 6 2512 1957
Fragments arranged by size:
2512 1957 6
NheI G'CTAG_C
Cuts at: 1113 1113
Size: 4475
NspI r_CATG'y
Cuts at: 891 1176 1528 2028 3631 891
Size: 285 352 500 1603 1735
Fragments arranged by size:
1735 1603 500 352 285
PasI CC'CwG_GG
Cuts at: 634 634
Size: 4475
PciI A'CATG_T
Cuts at: 1172 3627 1172
Size: 2455 2020
PflMI CCAn_nnn'nTGG
Cuts at: 977 977
Size: 4475
PmeI GTTT'AAAC
Cuts at: 1474 1474
Size: 4475
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 337 369 2921 2953 337
Size: 32 2552 32 1859
Fragments arranged by size:
2552 1859 32 32
PshAI GACnn'nnGTC
Cuts at: 1238 1238
Size: 4475
PsiI TTA'TAA
Cuts at: 266 266
Size: 4475
PstI C_TGCA'G
Cuts at: 585 1320 1522 2207 585
Size: 735 202 685 2853
Fragments arranged by size:
2853 735 685 202
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4475
PvuII CAG'CTG
Cuts at: 150 208 1315 1900 2260 150
Size: 58 1107 585 360 2365
Fragments arranged by size:
2365 1107 585 360 58
RsrII CG'GwC_CG
Cuts at: 1074 2670 1074
Size: 1596 2879
SacI G_AGCT'C
Cuts at: 1489 1489
Size: 4475
SacII CC_GC'GG
Cuts at: 1138 1138
Size: 4475
SalI G'TCGA_C
Cuts at: 1512 1512
Size: 4475
SapI GCTCTTCn'nnn_
Cuts at: 160 2498 2708 160
Size: 2338 210 1927
Fragments arranged by size:
2338 1927 210
SbfI CC_TGCA'GG
Cuts at: 585 1522 585
Size: 937 3538
ScaI AGT'ACT
Cuts at: 3992 3992
Size: 4475
SfcI C'TryA_G
Cuts at: 33 444 581 1316 1518 2203 3171 3362 33
Size: 411 137 735 202 685 968 191 1146
Fragments arranged by size:
1146 968 735 685 411 202 191 137
SfoI GGC'GCC
Cuts at: 141 1392 1907 2155 141
Size: 1251 515 248 2461
Fragments arranged by size:
2461 1251 515 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4475
SmaI CCC'GGG
Cuts at: 1497 1497
Size: 4475
SmlI C'TyrA_G
Cuts at: 937 1630 2545 3259 3521 937
Size: 693 915 714 262 1891
Fragments arranged by size:
1891 915 714 693 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4475
SphI G_CATG'C
Cuts at: 1528 2028 1528
Size: 500 3975
StuI AGG'CCT
Cuts at: 587 587
Size: 4475
StyI C'CwwG_G
Cuts at: 394 1609 1963 394
Size: 1215 354 2906
Fragments arranged by size:
2906 1215 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1138 1366 1375 2058 2191 2650 2859 1138
Size: 228 9 683 133 459 209 2754
Fragments arranged by size:
2754 683 459 228 209 133 9
TatI w'GTAC_w
Cuts at: 518 1169 1175 3990 518
Size: 651 6 2815 1003
Fragments arranged by size:
2815 1003 651 6
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 319 351 3992 4024 319
Size: 32 3641 32 770
Fragments arranged by size:
3641 770 32 32
Tth111I GACn'n_nGTC
Cuts at: 2272 2272
Size: 4475
XbaI T'CTAG_A
Cuts at: 1506 1506
Size: 4475
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 366 462 1260 1970 366
Size: 96 798 710 2871
Fragments arranged by size:
2871 798 710 96
XhoI C'TCGA_G
Cuts at: 937 937
Size: 4475
XmaI C'CCGG_G
Cuts at: 1495 1495
Size: 4475
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AleI AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglII BlpI
Bme1580I BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NheI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AlfI ApaI
AscI AvrII BbvCI BfrBI BglI BmgBI Bpu10I BsiWI
BspEI BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII
EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI
NotI NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI XmnI ZraI