Restriction map for
pF1KB8658
(Circular) (Six-base) MAPSORT of: pF1KB8658.seq Check: 5705 from: 1 to: 4673
pF1KB8658 4673 bp
With 182 enzymes: SgfI *
September 18, 2009 17:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1668 1668
Size: 4673
AatII G_ACGT'C
Cuts at: 1536 1536
Size: 4673
AccI GT'mk_AC
Cuts at: 479 932 1530 1711 479
Size: 453 598 181 3441
Fragments arranged by size:
3441 598 453 181
Acc65I G'GTAC_C
Cuts at: 1689 1689
Size: 4673
AclI AA'CG_TT
Cuts at: 4513 4513
Size: 4673
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 947 2498 2930 3677 3945 947
Size: 1551 432 747 268 1675
Fragments arranged by size:
1675 1551 747 432 268
AflIII A'CryG_T
Cuts at: 2025 3129 4106 2025
Size: 1104 977 2592
Fragments arranged by size:
2592 1104 977
AgeI A'CCGG_T
Cuts at: 3061 3061
Size: 4673
AlwNI CAG_nnn'CTG
Cuts at: 412 1978 3545 412
Size: 1566 1567 1540
Fragments arranged by size:
1567 1566 1540
ApaLI G'TGCA_C
Cuts at: 3443 3443
Size: 4673
ApoI r'AATT_y
Cuts at: 1677 4026 4282 1677
Size: 2349 256 2068
Fragments arranged by size:
2349 2068 256
AseI AT'TA_AT
Cuts at: 20 503 20
Size: 483 4190
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4673
AvaI C'yCGr_G
Cuts at: 1693 1693
Size: 4673
BamHI G'GATC_C
Cuts at: 1698 1698
Size: 4673
BanI G'GyrC_C
Cuts at: 1689 2103 2351 2386 1689
Size: 414 248 35 3976
Fragments arranged by size:
3976 414 248 35
BanII G_rGCy'C
Cuts at: 978 1687 2717 978
Size: 709 1030 2934
Fragments arranged by size:
2934 1030 709
BbeI G_GCGC'C
Cuts at: 2107 2355 2107
Size: 248 4425
BbsI GAAGACnn'nnnn_
Cuts at: 1505 1505
Size: 4673
BciVI GTATCCnnnnn_n'
Cuts at: 638 2571 3332 4673 638
Size: 1933 761 1341 638
Fragments arranged by size:
1933 1341 761 638
BclI T'GATC_A
Cuts at: 1353 2193 1353
Size: 840 3833
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 4673
BglII A'GATC_T
Cuts at: 916 2188 4225 916
Size: 1272 2037 1364
Fragments arranged by size:
2037 1364 1272
BlpI GC'TnA_GC
Cuts at: 145 1785 145
Size: 1640 3033
Bme1580I G_kGCm'C
Cuts at: 2298 2391 3447 2298
Size: 93 1056 3524
Fragments arranged by size:
3524 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 800 1885 1966 2301 4445 800
Size: 1085 81 335 2144 1028
Fragments arranged by size:
2144 1085 1028 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 532 3040 532
Size: 2508 2165
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 953 953
Size: 4673
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1849 2728 3220 3518 1849
Size: 879 492 298 3004
Fragments arranged by size:
3004 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 295 1292 4377 295
Size: 997 3085 591
Fragments arranged by size:
3085 997 591
BsaAI yAC'GTr
Cuts at: 1150 2026 1150
Size: 876 3797
BsaBI GATnn'nnATC
Cuts at: 6 2187 4224 6
Size: 2181 2037 455
Fragments arranged by size:
2181 2037 455
BsaHI Gr'CG_yC
Cuts at: 1533 2104 2352 3054 4550 1533
Size: 571 248 702 1496 1656
Fragments arranged by size:
1656 1496 702 571 248
BsaWI w'CCGG_w
Cuts at: 157 1862 2383 3061 3335 3482 3893 157
Size: 1705 521 678 274 147 411 937
Fragments arranged by size:
1705 937 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 163 193 387 417 756 786 163
Size: 30 194 30 339 30 4050
Fragments arranged by size:
4050 339 194 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 184 761 968 989 1007 1058 184
Size: 577 207 21 18 51 3799
Fragments arranged by size:
3799 577 207 51 21 18
BseYI C'CCAG_C
Cuts at: 2098 2856 3433 3929 2098
Size: 758 577 496 2842
Fragments arranged by size:
2842 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1130 1130
Size: 4673
BsiEI CG_ry'CG
Cuts at: 104 116 2215 2261 3469 104
Size: 12 2099 46 1208 1308
Fragments arranged by size:
2099 1308 1208 46 12
BsiHKAI G_wGCw'C
Cuts at: 384 978 1648 1687 2465 2655 3447 384
Size: 594 670 39 778 190 792 1610
Fragments arranged by size:
1610 792 778 670 594 190 39
Bsp1286I G_dGCh'C
Cuts at: 384 978 1648 1687 2298 2391 2465 2655 2717
Size: 594 670 39 611 93 74 190 62
Cuts at: 2717 3447 384
Size: 730 1610
Fragments arranged by size:
1610 730 670 611 594 190 93 74
62 39
BspEI T'CCGG_A
Cuts at: 3893 3893
Size: 4673
BspHI T'CATG_A
Cuts at: 3849 3849
Size: 4673
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1668 1723 1937 2239 2620 1668
Size: 55 214 302 381 3721
Fragments arranged by size:
3721 381 302 214 55
BsrBI CCG'CTC
Cuts at: 50 149 2965 3019 4503 4668 50
Size: 99 2816 54 1484 165 55
Fragments arranged by size:
2816 1484 165 99 55 54
BsrDI GCAATG_nn'
Cuts at: 1173 2585 1173
Size: 1412 3261
BsrFI r'CCGG_y
Cuts at: 115 2671 2852 3061 115
Size: 2556 181 209 1727
Fragments arranged by size:
2556 1727 209 181
BssSI C'ACGA_G
Cuts at: 2944 3302 2944
Size: 358 4315
BstAPI GCAn_nnn'nTGC
Cuts at: 2050 2050
Size: 4673
BstBI TT'CG_AA
Cuts at: 3034 3034
Size: 4673
BstXI CCAn_nnnn'nTGG
Cuts at: 1189 1189
Size: 4673
BstYI r'GATC_y
Cuts at: 1 916 1698 2165 2188 2523 2769 3781 3867
Size: 915 782 467 23 335 246 1012 86
Cuts at: 3867 3879 4225 1
Size: 12 346 449
Fragments arranged by size:
1012 915 782 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 553 553
Size: 4673
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 206 344 1400 2623 2804 2967 206
Size: 138 1056 1223 181 163 1912
Fragments arranged by size:
1912 1223 1056 181 163 138
BtsI GCAGTG_nn'
Cuts at: 1031 1240 1626 1916 1031
Size: 209 386 290 3788
Fragments arranged by size:
3788 386 290 209
DraI TTT'AAA
Cuts at: 1672 1672
Size: 4673
DraIII CAC_nnn'GTG
Cuts at: 1372 1372
Size: 4673
DrdI GACnn_nn'nnGTC
Cuts at: 2379 3237 2379
Size: 858 3815
EaeI y'GGCC_r
Cuts at: 113 2258 2432 2823 2850 4195 113
Size: 2145 174 391 27 1345 591
Fragments arranged by size:
2145 1345 591 391 174 27
EagI C'GGCC_G
Cuts at: 113 2258 113
Size: 2145 2528
EarI CTCTTCn'nnn_
Cuts at: 170 435 1248 2696 2906 170
Size: 265 813 1448 210 1937
Fragments arranged by size:
1937 1448 813 265 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2881 3191 3337 4483 2881
Size: 310 146 1146 3071
Fragments arranged by size:
3071 1146 310 146
EcoICRI GAG'CTC
Cuts at: 976 1685 976
Size: 709 3964
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 947 953 2498 2930 3677 3945 947
Size: 6 1545 432 747 268 1675
Fragments arranged by size:
1675 1545 747 432 268 6
EcoNI CCTnn'n_nnAGG
Cuts at: 1073 1073
Size: 4673
EcoO109I rG'GnC_Cy
Cuts at: 903 1812 903
Size: 909 3764
EcoRI G'AATT_C
Cuts at: 1677 1677
Size: 4673
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2692 2692
Size: 4673
FspI TGC'GCA
Cuts at: 2454 2454
Size: 4673
HaeII r_GCGC'y
Cuts at: 132 1082 2107 2355 3377 4356 132
Size: 950 1025 248 1022 979 449
Fragments arranged by size:
1025 1022 979 950 449 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2515 2545 2547 2577 2515
Size: 30 2 30 4611
Fragments arranged by size:
4611 30 30 2
HincII GTy'rAC
Cuts at: 1712 1712
Size: 4673
HindIII A'AGCT_T
Cuts at: 620 620
Size: 4673
KasI G'GCGC_C
Cuts at: 2103 2351 2103
Size: 248 4425
KpnI G_GTAC'C
Cuts at: 1693 1693
Size: 4673
MfeI C'AATT_G
Cuts at: 824 824
Size: 4673
MluI A'CGCG_T
Cuts at: 4106 4106
Size: 4673
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 119 1045 3344 3528 119
Size: 926 2299 184 1264
Fragments arranged by size:
2299 1264 926 184
MscI TGG'CCA
Cuts at: 2434 2434
Size: 4673
MslI CAynn'nnrTG
Cuts at: 1544 2662 2789 1544
Size: 1118 127 3428
Fragments arranged by size:
3428 1118 127
MspA1I CmG'CkG
Cuts at: 1784 2098 2458 3471 3716 1784
Size: 314 360 1013 245 2741
Fragments arranged by size:
2741 1013 360 314 245
NaeI GCC'GGC
Cuts at: 117 2854 117
Size: 2737 1936
NarI GG'CG_CC
Cuts at: 2104 2352 2104
Size: 248 4425
NcoI C'CATG_G
Cuts at: 553 553
Size: 4673
NdeI CA'TA_TG
Cuts at: 1161 1161
Size: 4673
NgoMIV G'CCGG_C
Cuts at: 115 2852 115
Size: 2737 1936
NspI r_CATG'y
Cuts at: 1726 2226 3133 1726
Size: 500 907 3266
Fragments arranged by size:
3266 907 500
PciI A'CATG_T
Cuts at: 3129 3129
Size: 4673
PflMI CCAn_nnn'nTGG
Cuts at: 391 391
Size: 4673
PfoI T'CCnGG_A
Cuts at: 969 969
Size: 4673
PmeI GTTT'AAAC
Cuts at: 1672 1672
Size: 4673
PmlI CAC'GTG
Cuts at: 1150 1150
Size: 4673
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3812 3844 3812
Size: 32 4641
PpuMI rG'GwC_Cy
Cuts at: 903 903
Size: 4673
PshAI GACnn'nnGTC
Cuts at: 466 1509 466
Size: 1043 3630
PstI C_TGCA'G
Cuts at: 1665 1720 2405 1665
Size: 55 685 3933
Fragments arranged by size:
3933 685 55
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4673
PvuII CAG'CTG
Cuts at: 2098 2458 2098
Size: 360 4313
RsrII CG'GwC_CG
Cuts at: 2868 2868
Size: 4673
SacI G_AGCT'C
Cuts at: 978 1687 978
Size: 709 3964
SalI G'TCGA_C
Cuts at: 1710 1710
Size: 4673
SapI GCTCTTCn'nnn_
Cuts at: 2696 2906 2696
Size: 210 4463
SbfI CC_TGCA'GG
Cuts at: 1720 1720
Size: 4673
ScaI AGT'ACT
Cuts at: 4190 4190
Size: 4673
SexAI A'CCwGG_T
Cuts at: 564 564
Size: 4673
SfcI C'TryA_G
Cuts at: 33 359 455 1661 1716 2401 3394 3585 33
Size: 326 96 1206 55 685 993 191 1121
Fragments arranged by size:
1206 1121 993 685 326 191 96 55
SfoI GGC'GCC
Cuts at: 2105 2353 2105
Size: 248 4425
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4673
SmaI CCC'GGG
Cuts at: 1695 1695
Size: 4673
SmlI C'TyrA_G
Cuts at: 1828 2743 3235 3497 1828
Size: 915 492 262 3004
Fragments arranged by size:
3004 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4673
SphI G_CATG'C
Cuts at: 1726 2226 1726
Size: 500 4173
StyI C'CwwG_G
Cuts at: 553 1807 2161 553
Size: 1254 354 3065
Fragments arranged by size:
3065 1254 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 319 826 1280 2256 2389 2848 3057 319
Size: 507 454 976 133 459 209 1935
Fragments arranged by size:
1935 976 507 459 454 209 133
TatI w'GTAC_w
Cuts at: 4188 4188
Size: 4673
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 163 195 975 1007 4190 4222 163
Size: 32 780 32 3183 32 614
Fragments arranged by size:
3183 780 614 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2470 2470
Size: 4673
XbaI T'CTAG_A
Cuts at: 1704 1704
Size: 4673
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2168 2168
Size: 4673
XmaI C'CCGG_G
Cuts at: 1693 1693
Size: 4673
ZraI GAC'GTC
Cuts at: 1534 1534
Size: 4673
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
ZraI
Enzymes that do not cut:
AfeI AflII AhdI AleI AlfI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI Bpu10I
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I
ClaI CspCI EcoRV FseI FspAI HpaI NheI NotI
NruI NsiI PacI PasI PsiI PspOMI PspXI PsrI
SacII SanDI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI