Restriction map for
pF1KB8660
(Circular) (Six-base) MAPSORT of: pF1KB8660.seq Check: 1368 from: 1 to: 4760
pF1KB8660 4760 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AatII G_ACGT'C
Cuts at: 1054 1054
Size: 4760
AccI GT'mk_AC
Cuts at: 1798 1798
Size: 4760
Acc65I G'GTAC_C
Cuts at: 1776 1776
Size: 4760
AclI AA'CG_TT
Cuts at: 4600 4600
Size: 4760
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 567 1647 2585 3017 3370 4032 567
Size: 1080 938 432 353 662 1295
Fragments arranged by size:
1295 1080 938 662 432 353
AflIII A'CryG_T
Cuts at: 968 2112 3912 4193 968
Size: 1144 1800 281 1535
Fragments arranged by size:
1800 1535 1144 281
AgeI A'CCGG_T
Cuts at: 1085 1085
Size: 4760
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1095 1095
Size: 4760
AlwNI CAG_nnn'CTG
Cuts at: 747 996 2065 3503 747
Size: 249 1069 1438 2004
Fragments arranged by size:
2004 1438 1069 249
ApaI G_GGCC'C
Cuts at: 433 1507 433
Size: 1074 3686
ApaLI G'TGCA_C
Cuts at: 3598 3598
Size: 4760
ApoI r'AATT_y
Cuts at: 308 1764 4113 4369 308
Size: 1456 2349 256 699
Fragments arranged by size:
2349 1456 699 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4760
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4760
AvaI C'yCGr_G
Cuts at: 943 1195 1780 943
Size: 252 585 3923
Fragments arranged by size:
3923 585 252
BamHI G'GATC_C
Cuts at: 1785 1785
Size: 4760
BanI G'GyrC_C
Cuts at: 1332 1654 1776 2190 2438 2473 1332
Size: 322 122 414 248 35 3619
Fragments arranged by size:
3619 414 322 248 122 35
BanII G_rGCy'C
Cuts at: 433 812 950 1507 1774 2804 433
Size: 379 138 557 267 1030 2389
Fragments arranged by size:
2389 1030 557 379 267 138
BbeI G_GCGC'C
Cuts at: 2194 2442 2194
Size: 248 4512
BbsI GAAGACnn'nnnn_
Cuts at: 1054 1252 1382 1054
Size: 198 130 4432
Fragments arranged by size:
4432 198 130
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1071 1105 1071
Size: 34 4726
BciVI GTATCCnnnnn_n'
Cuts at: 2658 3714 4760 2658
Size: 1056 1046 2658
Fragments arranged by size:
2658 1056 1046
BclI T'GATC_A
Cuts at: 2280 2280
Size: 4760
BfrBI ATG'CAT
Cuts at: 724 724
Size: 4760
BglI GCCn_nnn'nGGC
Cuts at: 791 1715 791
Size: 924 3836
BglII A'GATC_T
Cuts at: 879 2275 4312 879
Size: 1396 2037 1327
Fragments arranged by size:
2037 1396 1327
BlpI GC'TnA_GC
Cuts at: 1872 1872
Size: 4760
Bme1580I G_kGCm'C
Cuts at: 144 433 735 1074 1335 1396 1409 1507 1657
Size: 289 302 339 261 61 13 98 150
Cuts at: 1657 2385 2478 3602 144
Size: 728 93 1124 1302
Fragments arranged by size:
1302 1124 728 339 302 289 261 150
98 93 61 13
BmgBI CAC'GTC
Cuts at: 114 842 969 114
Size: 728 127 3905
Fragments arranged by size:
3905 728 127
BmrI ACTGGGnnnn_n'
Cuts at: 369 1972 2053 2388 4532 369
Size: 1603 81 335 2144 597
Fragments arranged by size:
2144 1603 597 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 780 3127 780
Size: 2347 2413
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1262 1467 1730 1262
Size: 205 263 4292
Fragments arranged by size:
4292 263 205
Bpu10I CC'TnA_GC
Cuts at: 215 676 1424 215
Size: 461 748 3551
Fragments arranged by size:
3551 748 461
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1111 1936 2815 3529 3827 1111
Size: 825 879 714 298 2044
Fragments arranged by size:
2044 879 825 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4464 4464
Size: 4760
BsaAI yAC'GTr
Cuts at: 1237 2113 1237
Size: 876 3884
BsaBI GATnn'nnATC
Cuts at: 6 2274 4311 6
Size: 2268 2037 455
Fragments arranged by size:
2268 2037 455
BsaHI Gr'CG_yC
Cuts at: 1051 2191 2439 3141 4637 1051
Size: 1140 248 702 1496 1174
Fragments arranged by size:
1496 1174 1140 702 248
BsaWI w'CCGG_w
Cuts at: 917 1085 1949 2470 3148 3559 3706 3980 917
Size: 168 864 521 678 411 147 274 1697
Fragments arranged by size:
1697 864 678 521 411 274 168 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 272 655 691 730 272
Size: 383 36 39 4302
Fragments arranged by size:
4302 383 39 36
BseYI C'CCAG_C
Cuts at: 190 351 1185 1408 1541 1633 2185 2943 3608
Size: 161 834 223 133 92 552 758 665
Cuts at: 3608 4016 190
Size: 408 934
Fragments arranged by size:
934 834 758 665 552 408 223 161
133 92
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 760 1613 760
Size: 853 3907
BsiEI CG_ry'CG
Cuts at: 104 546 2302 2348 3578 104
Size: 442 1756 46 1230 1286
Fragments arranged by size:
1756 1286 1230 442 46
BsiHKAI G_wGCw'C
Cuts at: 530 1127 1692 1774 2552 2742 3602 530
Size: 597 565 82 778 190 860 1688
Fragments arranged by size:
1688 860 778 597 565 190 82
BsmI GAATG_Cn'
Cuts at: 416 724 416
Size: 308 4452
BsmBI CGTCTCn'nnnn_
Cuts at: 262 262
Size: 4760
Bsp1286I G_dGCh'C
Cuts at: 144 433 530 735 812 950 1074 1127 1335
Size: 289 97 205 77 138 124 53 208
Cuts at: 1335 1396 1409 1507 1657 1692 1774 2385 2478
Size: 61 13 98 150 35 82 611 93
Cuts at: 2478 2552 2742 2804 3602 144
Size: 74 190 62 798 1302
Fragments arranged by size:
1302 798 611 289 208 205 190 150
138 124 98 97 93 82 77 74
62 61 53 35 13
BspEI T'CCGG_A
Cuts at: 917 917
Size: 4760
BspHI T'CATG_A
Cuts at: 497 3192 497
Size: 2695 2065
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1184 1810 2024 2326 2707 1184
Size: 626 214 302 381 3237
Fragments arranged by size:
3237 626 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1608 3052 3106 4590 4755 50
Size: 1558 1444 54 1484 165 55
Fragments arranged by size:
1558 1484 1444 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2672 2672
Size: 4760
BsrFI r'CCGG_y
Cuts at: 1085 1227 2758 2939 1085
Size: 142 1531 181 2906
Fragments arranged by size:
2906 1531 181 142
BsrGI T'GTAC_A
Cuts at: 261 261
Size: 4760
BssSI C'ACGA_G
Cuts at: 3031 3739 3031
Size: 708 4052
BstAPI GCAn_nnn'nTGC
Cuts at: 768 2137 768
Size: 1369 3391
BstBI TT'CG_AA
Cuts at: 225 3121 225
Size: 2896 1864
BstXI CCAn_nnnn'nTGG
Cuts at: 114 858 114
Size: 744 4016
BstYI r'GATC_y
Cuts at: 1 879 935 1785 2252 2275 2610 2856 3162
Size: 878 56 850 467 23 335 246 306
Cuts at: 3162 3174 3260 4312 1
Size: 12 86 1052 449
Fragments arranged by size:
1052 878 850 467 449 335 306 246
86 56 23 12
Bsu36I CC'TnA_GG
Cuts at: 713 1436 1698 713
Size: 723 262 3775
Fragments arranged by size:
3775 723 262
BtgI C'CryG_G
Cuts at: 107 857 1066 1300 1327 107
Size: 750 209 234 27 3540
Fragments arranged by size:
3540 750 234 209 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 669 2710 2891 3054 669
Size: 2041 181 163 2375
Fragments arranged by size:
2375 2041 181 163
BtsI GCAGTG_nn'
Cuts at: 898 1465 1690 2003 898
Size: 567 225 313 3655
Fragments arranged by size:
3655 567 313 225
ClaI AT'CG_AT
Cuts at: 591 591
Size: 4760
DraI TTT'AAA
Cuts at: 1759 1759
Size: 4760
DrdI GACnn_nn'nnGTC
Cuts at: 2466 3810 2466
Size: 1344 3416
EaeI y'GGCC_r
Cuts at: 749 2345 2519 2910 2937 4282 749
Size: 1596 174 391 27 1345 1227
Fragments arranged by size:
1596 1345 1227 391 174 27
EagI C'GGCC_G
Cuts at: 2345 2345
Size: 4760
EarI CTCTTCn'nnn_
Cuts at: 2783 2993 2783
Size: 210 4550
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1504 2968 3710 3856 4570 1504
Size: 1464 742 146 714 1694
Fragments arranged by size:
1694 1464 742 714 146
EcoICRI GAG'CTC
Cuts at: 1772 1772
Size: 4760
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 567 1262 1467 1647 1730 2585 3017 3370 4032
Size: 695 205 180 83 855 432 353 662
Cuts at: 4032 567
Size: 1295
Fragments arranged by size:
1295 855 695 662 432 353 205 180
83
EcoNI CCTnn'n_nnAGG
Cuts at: 1104 1255 1423 1104
Size: 151 168 4441
Fragments arranged by size:
4441 168 151
EcoO109I rG'GnC_Cy
Cuts at: 211 429 672 1139 1173 1272 1432 1503 1504
Size: 218 243 467 34 99 160 71 1
Cuts at: 1504 1702 1899 211
Size: 198 197 3072
Fragments arranged by size:
3072 467 243 218 198 197 160 99
71 34 1
EcoRI G'AATT_C
Cuts at: 1764 1764
Size: 4760
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2779 2779
Size: 4760
FspI TGC'GCA
Cuts at: 2541 2541
Size: 4760
HaeII r_GCGC'y
Cuts at: 997 1041 2194 2442 3672 4443 997
Size: 44 1153 248 1230 771 1314
Fragments arranged by size:
1314 1230 1153 771 248 44
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 143 175 479 511 922 954 1189 1221 2602
Size: 32 304 32 411 32 235 32 1381
Cuts at: 2602 2632 2634 2664 143
Size: 30 2 30 2239
Fragments arranged by size:
2239 1381 411 304 235 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1799 1799
Size: 4760
KasI G'GCGC_C
Cuts at: 2190 2438 2190
Size: 248 4512
KpnI G_GTAC'C
Cuts at: 1780 1780
Size: 4760
MluI A'CGCG_T
Cuts at: 4193 4193
Size: 4760
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1670 3519 3703 1670
Size: 1849 184 2727
Fragments arranged by size:
2727 1849 184
MscI TGG'CCA
Cuts at: 751 2521 751
Size: 1770 2990
MslI CAynn'nnrTG
Cuts at: 112 163 2749 2876 112
Size: 51 2586 127 1996
Fragments arranged by size:
2586 1996 127 51
MspA1I CmG'CkG
Cuts at: 609 744 1302 1572 1871 2185 2545 3329 3574
Size: 135 558 270 299 314 360 784 245
Cuts at: 3574 609
Size: 1795
Fragments arranged by size:
1795 784 558 360 314 299 270 245
135
NaeI GCC'GGC
Cuts at: 1229 2941 1229
Size: 1712 3048
NarI GG'CG_CC
Cuts at: 2191 2439 2191
Size: 248 4512
NcoI C'CATG_G
Cuts at: 107 857 107
Size: 750 4010
NdeI CA'TA_TG
Cuts at: 636 636
Size: 4760
NgoMIV G'CCGG_C
Cuts at: 1227 2939 1227
Size: 1712 3048
NsiI A_TGCA'T
Cuts at: 726 726
Size: 4760
NspI r_CATG'y
Cuts at: 832 1813 2313 3916 832
Size: 981 500 1603 1676
Fragments arranged by size:
1676 1603 981 500
PciI A'CATG_T
Cuts at: 3912 3912
Size: 4760
PfoI T'CCnGG_A
Cuts at: 1370 1444 1370
Size: 74 4686
PmeI GTTT'AAAC
Cuts at: 1759 1759
Size: 4760
PmlI CAC'GTG
Cuts at: 1237 1237
Size: 4760
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3206 3238 3206
Size: 32 4728
PpuMI rG'GwC_Cy
Cuts at: 672 1173 1432 672
Size: 501 259 4000
Fragments arranged by size:
4000 501 259
PspOMI G'GGCC_C
Cuts at: 429 1503 429
Size: 1074 3686
PstI C_TGCA'G
Cuts at: 670 773 1460 1807 2492 670
Size: 103 687 347 685 2938
Fragments arranged by size:
2938 687 685 347 103
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4760
PvuII CAG'CTG
Cuts at: 744 1572 2185 2545 744
Size: 828 613 360 2959
Fragments arranged by size:
2959 828 613 360
RsrII CG'GwC_CG
Cuts at: 2955 2955
Size: 4760
SacI G_AGCT'C
Cuts at: 1774 1774
Size: 4760
SacII CC_GC'GG
Cuts at: 1303 1303
Size: 4760
SalI G'TCGA_C
Cuts at: 1797 1797
Size: 4760
SapI GCTCTTCn'nnn_
Cuts at: 2783 2993 2783
Size: 210 4550
SbfI CC_TGCA'GG
Cuts at: 773 1807 773
Size: 1034 3726
ScaI AGT'ACT
Cuts at: 4277 4277
Size: 4760
SfcI C'TryA_G
Cuts at: 33 666 769 1456 1803 2488 3456 3647 33
Size: 633 103 687 347 685 968 191 1146
Fragments arranged by size:
1146 968 687 685 633 347 191 103
SfoI GGC'GCC
Cuts at: 2192 2440 2192
Size: 248 4512
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4760
SmaI CCC'GGG
Cuts at: 1782 1782
Size: 4760
SmlI C'TyrA_G
Cuts at: 1126 1915 2830 3544 3806 1126
Size: 789 915 714 262 2080
Fragments arranged by size:
2080 915 789 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4760
SphI G_CATG'C
Cuts at: 1813 2313 1813
Size: 500 4260
StuI AGG'CCT
Cuts at: 1221 1221
Size: 4760
StyI C'CwwG_G
Cuts at: 107 857 1507 1894 2248 107
Size: 750 650 387 354 2619
Fragments arranged by size:
2619 750 650 387 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 330 1647 2343 2476 2935 3144 330
Size: 1317 696 133 459 209 1946
Fragments arranged by size:
1946 1317 696 459 209 133
TatI w'GTAC_w
Cuts at: 261 644 4275 261
Size: 383 3631 746
Fragments arranged by size:
3631 746 383
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1338 1370 4277 4309 1338
Size: 32 2907 32 1789
Fragments arranged by size:
2907 1789 32 32
Tth111I GACn'n_nGTC
Cuts at: 2557 2557
Size: 4760
XbaI T'CTAG_A
Cuts at: 1791 1791
Size: 4760
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1416 2255 1416
Size: 839 3921
XmaI C'CCGG_G
Cuts at: 1780 1780
Size: 4760
ZraI GAC'GTC
Cuts at: 1052 1052
Size: 4760
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NsiI NspI PciI PfoI
PmeI PmlI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AloI AscI AvrII
BaeI BbvCI BmtI BsaXI BsiWI BssHII BstEII BstZ17I
CspCI DraIII EcoRV FseI FspAI HindIII HpaI MfeI
NheI NotI NruI PacI PasI PflMI PshAI PsiI
PspXI PsrI SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI XmnI