Restriction map for
pF1KB8662
(Circular) (Six-base) MAPSORT of: pF1KB8662.seq Check: 2 from: 1 to: 4244
pF1KB8662 4244 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AccI GT'mk_AC
Cuts at: 1282 1282
Size: 4244
Acc65I G'GTAC_C
Cuts at: 1260 1260
Size: 4244
AclI AA'CG_TT
Cuts at: 4084 4084
Size: 4244
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2069 2501 2854 3516 2069
Size: 432 353 662 2797
Fragments arranged by size:
2797 662 432 353
AflIII A'CryG_T
Cuts at: 1596 3396 3677 1596
Size: 1800 281 2163
Fragments arranged by size:
2163 1800 281
AlwNI CAG_nnn'CTG
Cuts at: 1549 2987 1549
Size: 1438 2806
ApaLI G'TGCA_C
Cuts at: 3082 3082
Size: 4244
ApoI r'AATT_y
Cuts at: 518 1248 3597 3853 518
Size: 730 2349 256 909
Fragments arranged by size:
2349 909 730 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4244
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4244
AvaI C'yCGr_G
Cuts at: 1264 1264
Size: 4244
BamHI G'GATC_C
Cuts at: 1269 1269
Size: 4244
BanI G'GyrC_C
Cuts at: 1059 1260 1674 1922 1957 1059
Size: 201 414 248 35 3346
Fragments arranged by size:
3346 414 248 201 35
BanII G_rGCy'C
Cuts at: 206 275 1258 2288 206
Size: 69 983 1030 2162
Fragments arranged by size:
2162 1030 983 69
BbeI G_GCGC'C
Cuts at: 1678 1926 1678
Size: 248 3996
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 528 562 528
Size: 34 4210
BciVI GTATCCnnnnn_n'
Cuts at: 2142 3198 4244 2142
Size: 1056 1046 2142
Fragments arranged by size:
2142 1056 1046
BclI T'GATC_A
Cuts at: 1764 1764
Size: 4244
BglII A'GATC_T
Cuts at: 938 1091 1759 3796 938
Size: 153 668 2037 1386
Fragments arranged by size:
2037 1386 668 153
BlpI GC'TnA_GC
Cuts at: 205 1356 205
Size: 1151 3093
Bme1580I G_kGCm'C
Cuts at: 1062 1869 1962 3086 1062
Size: 807 93 1124 2220
Fragments arranged by size:
2220 1124 807 93
BmrI ACTGGGnnnn_n'
Cuts at: 1456 1537 1872 4016 1456
Size: 81 335 2144 1684
Fragments arranged by size:
2144 1684 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2611 2611
Size: 4244
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 184 289 1129 184
Size: 105 840 3299
Fragments arranged by size:
3299 840 105
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 430 826 1420 2299 3013 3311 430
Size: 396 594 879 714 298 1363
Fragments arranged by size:
1363 879 714 594 396 298
BsaI GGTCTCn'nnnn_
Cuts at: 3948 3948
Size: 4244
BsaAI yAC'GTr
Cuts at: 1597 1597
Size: 4244
BsaBI GATnn'nnATC
Cuts at: 6 357 1758 3795 6
Size: 351 1401 2037 455
Fragments arranged by size:
2037 1401 455 351
BsaHI Gr'CG_yC
Cuts at: 1675 1923 2625 4121 1675
Size: 248 702 1496 1798
Fragments arranged by size:
1798 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1034 1433 1954 2632 3043 3190 3464 1034
Size: 399 521 678 411 147 274 1814
Fragments arranged by size:
1814 678 521 411 399 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 214 523 1009 214
Size: 309 486 3449
Fragments arranged by size:
3449 486 309
BseYI C'CCAG_C
Cuts at: 839 1231 1669 2427 3092 3500 839
Size: 392 438 758 665 408 1583
Fragments arranged by size:
1583 758 665 438 408 392
BsiEI CG_ry'CG
Cuts at: 104 1786 1832 3062 104
Size: 1682 46 1230 1286
Fragments arranged by size:
1682 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 206 1258 2036 2226 3086 206
Size: 1052 778 190 860 1364
Fragments arranged by size:
1364 1052 860 778 190
Bsp1286I G_dGCh'C
Cuts at: 206 275 1062 1258 1869 1962 2036 2226 2288
Size: 69 787 196 611 93 74 190 62
Cuts at: 2288 3086 206
Size: 798 1364
Fragments arranged by size:
1364 798 787 611 196 190 93 74
69 62
BspEI T'CCGG_A
Cuts at: 1034 1034
Size: 4244
BspHI T'CATG_A
Cuts at: 2676 2676
Size: 4244
BspMI ACCTGCnnnn'nnnn_
Cuts at: 491 861 1294 1508 1810 2191 491
Size: 370 433 214 302 381 2544
Fragments arranged by size:
2544 433 381 370 302 214
BsrBI CCG'CTC
Cuts at: 50 2536 2590 4074 4239 50
Size: 2486 54 1484 165 55
Fragments arranged by size:
2486 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 914 2156 914
Size: 1242 3002
BsrFI r'CCGG_y
Cuts at: 2242 2423 2242
Size: 181 4063
BssSI C'ACGA_G
Cuts at: 493 2515 3223 493
Size: 2022 708 1514
Fragments arranged by size:
2022 1514 708
BstAPI GCAn_nnn'nTGC
Cuts at: 328 1621 328
Size: 1293 2951
BstBI TT'CG_AA
Cuts at: 2605 2605
Size: 4244
BstYI r'GATC_y
Cuts at: 1 352 938 1091 1269 1736 1759 2094 2340
Size: 351 586 153 178 467 23 335 246
Cuts at: 2340 2646 2658 2744 3796 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1052 586 467 449 351 335 306 246
178 153 86 23 12
BtgI C'CryG_G
Cuts at: 378 378
Size: 4244
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2194 2375 2538 2194
Size: 181 163 3900
Fragments arranged by size:
3900 181 163
BtsI GCAGTG_nn'
Cuts at: 1025 1487 1025
Size: 462 3782
DraI TTT'AAA
Cuts at: 1243 1243
Size: 4244
DrdI GACnn_nn'nnGTC
Cuts at: 1950 3294 1950
Size: 1344 2900
EaeI y'GGCC_r
Cuts at: 605 1046 1829 2003 2394 2421 3766 605
Size: 441 783 174 391 27 1345 1083
Fragments arranged by size:
1345 1083 783 441 391 174 27
EagI C'GGCC_G
Cuts at: 1829 1829
Size: 4244
EarI CTCTTCn'nnn_
Cuts at: 734 1108 2267 2477 734
Size: 374 1159 210 2501
Fragments arranged by size:
2501 1159 374 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2452 3194 3340 4054 2452
Size: 742 146 714 2642
Fragments arranged by size:
2642 742 714 146
EcoICRI GAG'CTC
Cuts at: 204 1256 204
Size: 1052 3192
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 184 289 1129 2069 2501 2854 3516 184
Size: 105 840 940 432 353 662 912
Fragments arranged by size:
940 912 840 662 432 353 105
EcoO109I rG'GnC_Cy
Cuts at: 753 1383 753
Size: 630 3614
EcoRI G'AATT_C
Cuts at: 1248 1248
Size: 4244
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 465 957 976 2263 465
Size: 492 19 1287 2446
Fragments arranged by size:
2446 1287 492 19
FspI TGC'GCA
Cuts at: 2025 2025
Size: 4244
HaeII r_GCGC'y
Cuts at: 1678 1926 3156 3927 1678
Size: 248 1230 771 1995
Fragments arranged by size:
1995 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 106 138 2086 2116 2118 2148 106
Size: 32 1948 30 2 30 2202
Fragments arranged by size:
2202 1948 32 30 30 2
HincII GTy'rAC
Cuts at: 1283 1283
Size: 4244
HindIII A'AGCT_T
Cuts at: 895 895
Size: 4244
KasI G'GCGC_C
Cuts at: 1674 1922 1674
Size: 248 3996
KpnI G_GTAC'C
Cuts at: 1264 1264
Size: 4244
MfeI C'AATT_G
Cuts at: 724 812 724
Size: 88 4156
MluI A'CGCG_T
Cuts at: 3677 3677
Size: 4244
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 137 164 3003 3187 137
Size: 27 2839 184 1194
Fragments arranged by size:
2839 1194 184 27
MscI TGG'CCA
Cuts at: 607 2005 607
Size: 1398 2846
MslI CAynn'nnrTG
Cuts at: 1123 2233 2360 1123
Size: 1110 127 3007
Fragments arranged by size:
3007 1110 127
MspA1I CmG'CkG
Cuts at: 183 1044 1355 1669 2029 2813 3058 183
Size: 861 311 314 360 784 245 1369
Fragments arranged by size:
1369 861 784 360 314 311 245
NaeI GCC'GGC
Cuts at: 2425 2425
Size: 4244
NarI GG'CG_CC
Cuts at: 1675 1923 1675
Size: 248 3996
NgoMIV G'CCGG_C
Cuts at: 2423 2423
Size: 4244
NspI r_CATG'y
Cuts at: 1297 1797 3400 1297
Size: 500 1603 2141
Fragments arranged by size:
2141 1603 500
PasI CC'CwG_GG
Cuts at: 275 275
Size: 4244
PciI A'CATG_T
Cuts at: 3396 3396
Size: 4244
PfoI T'CCnGG_A
Cuts at: 361 1082 361
Size: 721 3523
PmeI GTTT'AAAC
Cuts at: 1243 1243
Size: 4244
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2690 2722 2690
Size: 32 4212
PshAI GACnn'nnGTC
Cuts at: 1078 1078
Size: 4244
PstI C_TGCA'G
Cuts at: 488 872 1291 1976 488
Size: 384 419 685 2756
Fragments arranged by size:
2756 685 419 384
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4244
PvuII CAG'CTG
Cuts at: 183 1044 1669 2029 183
Size: 861 625 360 2398
Fragments arranged by size:
2398 861 625 360
RsrII CG'GwC_CG
Cuts at: 2439 2439
Size: 4244
SacI G_AGCT'C
Cuts at: 206 1258 206
Size: 1052 3192
SalI G'TCGA_C
Cuts at: 1281 1281
Size: 4244
SapI GCTCTTCn'nnn_
Cuts at: 1108 2267 2477 1108
Size: 1159 210 2875
Fragments arranged by size:
2875 1159 210
SbfI CC_TGCA'GG
Cuts at: 1291 1291
Size: 4244
ScaI AGT'ACT
Cuts at: 3761 3761
Size: 4244
SfcI C'TryA_G
Cuts at: 33 484 868 1287 1972 2940 3131 33
Size: 451 384 419 685 968 191 1146
Fragments arranged by size:
1146 968 685 451 419 384 191
SfoI GGC'GCC
Cuts at: 1676 1924 1676
Size: 248 3996
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4244
SmaI CCC'GGG
Cuts at: 1266 1266
Size: 4244
SmlI C'TyrA_G
Cuts at: 409 805 1399 2314 3028 3290 409
Size: 396 594 915 714 262 1363
Fragments arranged by size:
1363 915 714 594 396 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4244
SphI G_CATG'C
Cuts at: 1297 1797 1297
Size: 500 3744
SspI AAT'ATT
Cuts at: 256 256
Size: 4244
StuI AGG'CCT
Cuts at: 389 389
Size: 4244
StyI C'CwwG_G
Cuts at: 1378 1732 1378
Size: 354 3890
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1827 1960 2419 2628 1827
Size: 133 459 209 3443
Fragments arranged by size:
3443 459 209 133
TatI w'GTAC_w
Cuts at: 3759 3759
Size: 4244
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3761 3793 3761
Size: 32 4212
Tth111I GACn'n_nGTC
Cuts at: 2041 2041
Size: 4244
XbaI T'CTAG_A
Cuts at: 1275 1275
Size: 4244
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1739 1739
Size: 4244
XmaI C'CCGG_G
Cuts at: 1264 1264
Size: 4244
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BcgI
BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PasI PciI PfoI PmeI
PpiI PshAI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
AloI ApaI AscI AvrII BaeI BbsI BbvCI BfrBI
BglI BmgBI BmtI Bpu10I BsaXI BsgI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HpaI NcoI
NdeI NheI NotI NruI NsiI PacI PflMI PmlI
PpuMI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SwaI XhoI XmnI ZraI