Restriction map for
pF1KB8663
(Circular) (Six-base) MAPSORT of: pF1KB8663.seq Check: 8382 from: 1 to: 4787
pF1KB8663 4787 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AccI GT'mk_AC
Cuts at: 1825 1825
Size: 4787
Acc65I G'GTAC_C
Cuts at: 1803 1803
Size: 4787
AclI AA'CG_TT
Cuts at: 4627 4627
Size: 4787
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 226 596 1430 2612 3044 3397 4059 226
Size: 370 834 1182 432 353 662 954
Fragments arranged by size:
1182 954 834 662 432 370 353
AflIII A'CryG_T
Cuts at: 2139 3939 4220 2139
Size: 1800 281 2706
Fragments arranged by size:
2706 1800 281
AlwNI CAG_nnn'CTG
Cuts at: 2092 3530 2092
Size: 1438 3349
ApaLI G'TGCA_C
Cuts at: 3625 3625
Size: 4787
ApoI r'AATT_y
Cuts at: 1482 1501 1791 4140 4396 1482
Size: 19 290 2349 256 1873
Fragments arranged by size:
2349 1873 290 256 19
AseI AT'TA_AT
Cuts at: 20 20
Size: 4787
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4787
AvaI C'yCGr_G
Cuts at: 1807 1807
Size: 4787
BamHI G'GATC_C
Cuts at: 1812 1812
Size: 4787
BanI G'GyrC_C
Cuts at: 662 1010 1803 2217 2465 2500 662
Size: 348 793 414 248 35 2949
Fragments arranged by size:
2949 793 414 348 248 35
BanII G_rGCy'C
Cuts at: 1801 2831 1801
Size: 1030 3757
BbeI G_GCGC'C
Cuts at: 2221 2469 2221
Size: 248 4539
BbsI GAAGACnn'nnnn_
Cuts at: 1436 1436
Size: 4787
BciVI GTATCCnnnnn_n'
Cuts at: 2685 3741 4787 2685
Size: 1056 1046 2685
Fragments arranged by size:
2685 1056 1046
BclI T'GATC_A
Cuts at: 1305 2307 1305
Size: 1002 3785
BfrBI ATG'CAT
Cuts at: 803 803
Size: 4787
BglII A'GATC_T
Cuts at: 642 724 2302 4339 642
Size: 82 1578 2037 1090
Fragments arranged by size:
2037 1578 1090 82
BlpI GC'TnA_GC
Cuts at: 1899 1899
Size: 4787
Bme1580I G_kGCm'C
Cuts at: 1001 2412 2505 3629 1001
Size: 1411 93 1124 2159
Fragments arranged by size:
2159 1411 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1313 1603 1999 2080 2415 4559 1313
Size: 290 396 81 335 2144 1541
Fragments arranged by size:
2144 1541 396 335 290 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1590 3154 1590
Size: 1564 3223
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1963 2842 3556 3854 1963
Size: 879 714 298 2896
Fragments arranged by size:
2896 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 214 922 1079 1644 4491 214
Size: 708 157 565 2847 510
Fragments arranged by size:
2847 708 565 510 157
BsaAI yAC'GTr
Cuts at: 2140 2140
Size: 4787
BsaBI GATnn'nnATC
Cuts at: 6 2301 4338 6
Size: 2295 2037 455
Fragments arranged by size:
2295 2037 455
BsaHI Gr'CG_yC
Cuts at: 2218 2466 3168 4664 2218
Size: 248 702 1496 2341
Fragments arranged by size:
2341 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1118 1976 2497 3175 3586 3733 4007 1118
Size: 858 521 678 411 147 274 1898
Fragments arranged by size:
1898 858 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 2212 2970 3635 4043 2212
Size: 758 665 408 2956
Fragments arranged by size:
2956 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 590 590
Size: 4787
BsiEI CG_ry'CG
Cuts at: 104 2329 2375 3605 104
Size: 2225 46 1230 1286
Fragments arranged by size:
2225 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1801 2579 2769 3629 1801
Size: 778 190 860 2959
Fragments arranged by size:
2959 860 778 190
BsmI GAATG_Cn'
Cuts at: 803 803
Size: 4787
Bsp1286I G_dGCh'C
Cuts at: 1001 1801 2412 2505 2579 2769 2831 3629 1001
Size: 800 611 93 74 190 62 798 2159
Fragments arranged by size:
2159 800 798 611 190 93 74 62
BspHI T'CATG_A
Cuts at: 3219 3219
Size: 4787
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1837 2051 2353 2734 1837
Size: 214 302 381 3890
Fragments arranged by size:
3890 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3079 3133 4617 4782 50
Size: 3029 54 1484 165 55
Fragments arranged by size:
3029 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2699 2699
Size: 4787
BsrFI r'CCGG_y
Cuts at: 2785 2966 2785
Size: 181 4606
BssSI C'ACGA_G
Cuts at: 3058 3766 3058
Size: 708 4079
BstAPI GCAn_nnn'nTGC
Cuts at: 313 2164 313
Size: 1851 2936
BstBI TT'CG_AA
Cuts at: 3148 3148
Size: 4787
BstEII G'GTnAC_C
Cuts at: 977 977
Size: 4787
BstXI CCAn_nnnn'nTGG
Cuts at: 1737 1737
Size: 4787
BstYI r'GATC_y
Cuts at: 1 642 724 1519 1812 2279 2302 2637 2883
Size: 641 82 795 293 467 23 335 246
Cuts at: 2883 3189 3201 3287 4339 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1052 795 641 467 449 335 306 293
246 86 82 23 12
Bsu36I CC'TnA_GG
Cuts at: 1658 1658
Size: 4787
BtgI C'CryG_G
Cuts at: 107 107
Size: 4787
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2737 2918 3081 2737
Size: 181 163 4443
Fragments arranged by size:
4443 181 163
BtsI GCAGTG_nn'
Cuts at: 876 1670 2030 876
Size: 794 360 3633
Fragments arranged by size:
3633 794 360
DraI TTT'AAA
Cuts at: 1786 1786
Size: 4787
DraIII CAC_nnn'GTG
Cuts at: 985 985
Size: 4787
DrdI GACnn_nn'nnGTC
Cuts at: 2493 3837 2493
Size: 1344 3443
EaeI y'GGCC_r
Cuts at: 1727 2372 2546 2937 2964 4309 1727
Size: 645 174 391 27 1345 2205
Fragments arranged by size:
2205 1345 645 391 174 27
EagI C'GGCC_G
Cuts at: 2372 2372
Size: 4787
EarI CTCTTCn'nnn_
Cuts at: 683 1171 1334 1355 2810 3020 683
Size: 488 163 21 1455 210 2450
Fragments arranged by size:
2450 1455 488 210 163 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2995 3737 3883 4597 2995
Size: 742 146 714 3185
Fragments arranged by size:
3185 742 714 146
EcoICRI GAG'CTC
Cuts at: 1799 1799
Size: 4787
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 226 596 1430 2612 3044 3397 4059 226
Size: 370 834 1182 432 353 662 954
Fragments arranged by size:
1182 954 834 662 432 370 353
EcoO109I rG'GnC_Cy
Cuts at: 300 1926 300
Size: 1626 3161
EcoRI G'AATT_C
Cuts at: 1791 1791
Size: 4787
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2806 2806
Size: 4787
FspI TGC'GCA
Cuts at: 2568 2568
Size: 4787
HaeII r_GCGC'y
Cuts at: 2221 2469 3699 4470 2221
Size: 248 1230 771 2538
Fragments arranged by size:
2538 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 208 240 2629 2659 2661 2691 208
Size: 32 2389 30 2 30 2304
Fragments arranged by size:
2389 2304 32 30 30 2
HincII GTy'rAC
Cuts at: 1826 1826
Size: 4787
KasI G'GCGC_C
Cuts at: 2217 2465 2217
Size: 248 4539
KpnI G_GTAC'C
Cuts at: 1807 1807
Size: 4787
MluI A'CGCG_T
Cuts at: 4220 4220
Size: 4787
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1547 3546 3730 1547
Size: 1999 184 2604
Fragments arranged by size:
2604 1999 184
MscI TGG'CCA
Cuts at: 1729 2548 1729
Size: 819 3968
MslI CAynn'nnrTG
Cuts at: 2776 2903 2776
Size: 127 4660
MspA1I CmG'CkG
Cuts at: 1898 2212 2572 3356 3601 1898
Size: 314 360 784 245 3084
Fragments arranged by size:
3084 784 360 314 245
NaeI GCC'GGC
Cuts at: 2968 2968
Size: 4787
NarI GG'CG_CC
Cuts at: 2218 2466 2218
Size: 248 4539
NcoI C'CATG_G
Cuts at: 107 107
Size: 4787
NdeI CA'TA_TG
Cuts at: 1367 1367
Size: 4787
NgoMIV G'CCGG_C
Cuts at: 2966 2966
Size: 4787
NsiI A_TGCA'T
Cuts at: 805 805
Size: 4787
NspI r_CATG'y
Cuts at: 620 1840 2340 3943 620
Size: 1220 500 1603 1464
Fragments arranged by size:
1603 1464 1220 500
PciI A'CATG_T
Cuts at: 3939 3939
Size: 4787
PmeI GTTT'AAAC
Cuts at: 1786 1786
Size: 4787
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3233 3265 3233
Size: 32 4755
PstI C_TGCA'G
Cuts at: 910 1834 2519 910
Size: 924 685 3178
Fragments arranged by size:
3178 924 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4787
PvuII CAG'CTG
Cuts at: 2212 2572 2212
Size: 360 4427
RsrII CG'GwC_CG
Cuts at: 2982 2982
Size: 4787
SacI G_AGCT'C
Cuts at: 1801 1801
Size: 4787
SalI G'TCGA_C
Cuts at: 1824 1824
Size: 4787
SapI GCTCTTCn'nnn_
Cuts at: 1355 2810 3020 1355
Size: 1455 210 3122
Fragments arranged by size:
3122 1455 210
SbfI CC_TGCA'GG
Cuts at: 1834 1834
Size: 4787
ScaI AGT'ACT
Cuts at: 4304 4304
Size: 4787
SfcI C'TryA_G
Cuts at: 33 906 1830 2515 3483 3674 33
Size: 873 924 685 968 191 1146
Fragments arranged by size:
1146 968 924 873 685 191
SfoI GGC'GCC
Cuts at: 2219 2467 2219
Size: 248 4539
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4787
SmaI CCC'GGG
Cuts at: 1809 1809
Size: 4787
SmlI C'TyrA_G
Cuts at: 1942 2857 3571 3833 1942
Size: 915 714 262 2896
Fragments arranged by size:
2896 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4787
SphI G_CATG'C
Cuts at: 1840 2340 1840
Size: 500 4287
SspI AAT'ATT
Cuts at: 1267 1267
Size: 4787
StyI C'CwwG_G
Cuts at: 107 1201 1921 2275 107
Size: 1094 720 354 2619
Fragments arranged by size:
2619 1094 720 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 965 2370 2503 2962 3171 965
Size: 1405 133 459 209 2581
Fragments arranged by size:
2581 1405 459 209 133
TatI w'GTAC_w
Cuts at: 4302 4302
Size: 4787
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4304 4336 4304
Size: 32 4755
Tth111I GACn'n_nGTC
Cuts at: 2584 2584
Size: 4787
XbaI T'CTAG_A
Cuts at: 1818 1818
Size: 4787
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 387 2282 387
Size: 1895 2892
XmaI C'CCGG_G
Cuts at: 1807 1807
Size: 4787
XmnI GAAnn'nnTTC
Cuts at: 239 943 239
Size: 704 4083
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BciVI BclI BfrBI BglII BlpI Bme1580I BmrI BplI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI
NspI PciI PmeI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
AloI ApaI AscI AvrII BaeI BbvCI BcgI BglI
BmgBI BmtI BpmI Bpu10I BsaXI BseRI BsiWI BsmBI
BspEI BsrGI BssHII BstZ17I ClaI CspCI EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NheI NotI NruI
PacI PasI PflMI PfoI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI StuI SwaI XhoI ZraI