Restriction map for
pF1KB8664
(Circular) (Six-base) MAPSORT of: pF1KB8664.seq Check: 5495 from: 1 to: 4340
pF1KB8664 4340 bp
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AatII G_ACGT'C
Cuts at: 270 270
Size: 4340
AccI GT'mk_AC
Cuts at: 1172 1256 1378 1172
Size: 84 122 4134
Fragments arranged by size:
4134 122 84
Acc65I G'GTAC_C
Cuts at: 1356 1356
Size: 4340
AclI AA'CG_TT
Cuts at: 4180 4180
Size: 4340
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 960 1057 2165 2597 2950 3612 960
Size: 97 1108 432 353 662 1688
Fragments arranged by size:
1688 1108 662 432 353 97
AflIII A'CryG_T
Cuts at: 1692 3492 3773 1692
Size: 1800 281 2259
Fragments arranged by size:
2259 1800 281
AlwNI CAG_nnn'CTG
Cuts at: 1645 3083 1645
Size: 1438 2902
ApaI G_GGCC'C
Cuts at: 1294 1294
Size: 4340
ApaLI G'TGCA_C
Cuts at: 3178 3178
Size: 4340
ApoI r'AATT_y
Cuts at: 427 613 1344 3693 3949 427
Size: 186 731 2349 256 818
Fragments arranged by size:
2349 818 731 256 186
AseI AT'TA_AT
Cuts at: 20 20
Size: 4340
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4340
AvaI C'yCGr_G
Cuts at: 1072 1360 1072
Size: 288 4052
BamHI G'GATC_C
Cuts at: 1365 1365
Size: 4340
BanI G'GyrC_C
Cuts at: 472 1356 1770 2018 2053 472
Size: 884 414 248 35 2759
Fragments arranged by size:
2759 884 414 248 35
BanII G_rGCy'C
Cuts at: 544 904 1080 1241 1294 1354 2384 544
Size: 360 176 161 53 60 1030 2500
Fragments arranged by size:
2500 1030 360 176 161 60 53
BbeI G_GCGC'C
Cuts at: 1774 2022 1774
Size: 248 4092
BbsI GAAGACnn'nnnn_
Cuts at: 789 1099 789
Size: 310 4030
BbvCI CC'TCA_GC
Cuts at: 200 994 200
Size: 794 3546
BciVI GTATCCnnnnn_n'
Cuts at: 2238 3294 4340 2238
Size: 1056 1046 2238
Fragments arranged by size:
2238 1056 1046
BclI T'GATC_A
Cuts at: 1860 1860
Size: 4340
BglII A'GATC_T
Cuts at: 1855 3892 1855
Size: 2037 2303
BlpI GC'TnA_GC
Cuts at: 1452 1452
Size: 4340
Bme1580I G_kGCm'C
Cuts at: 1294 1965 2058 3182 1294
Size: 671 93 1124 2452
Fragments arranged by size:
2452 1124 671 93
BmrI ACTGGGnnnn_n'
Cuts at: 1172 1552 1633 1968 4112 1172
Size: 380 81 335 2144 1400
Fragments arranged by size:
2144 1400 380 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2707 2707
Size: 4340
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 208 208
Size: 4340
Bpu10I CC'TnA_GC
Cuts at: 200 232 994 200
Size: 32 762 3546
Fragments arranged by size:
3546 762 32
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 262 1516 2395 3109 3407 262
Size: 1254 879 714 298 1195
Fragments arranged by size:
1254 1195 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1035 4044 1035
Size: 3009 1331
BsaAI yAC'GTr
Cuts at: 1693 1693
Size: 4340
BsaBI GATnn'nnATC
Cuts at: 6 919 1854 3891 6
Size: 913 935 2037 455
Fragments arranged by size:
2037 935 913 455
BsaHI Gr'CG_yC
Cuts at: 267 1771 2019 2721 4217 267
Size: 1504 248 702 1496 390
Fragments arranged by size:
1504 1496 702 390 248
BsaWI w'CCGG_w
Cuts at: 1262 1529 2050 2728 3139 3286 3560 1262
Size: 267 521 678 411 147 274 2042
Fragments arranged by size:
2042 678 521 411 274 267 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1315 1345 1315
Size: 30 4310
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 127 586 807 951 1336 127
Size: 459 221 144 385 3131
Fragments arranged by size:
3131 459 385 221 144
BseYI C'CCAG_C
Cuts at: 904 1124 1765 2523 3188 3596 904
Size: 220 641 758 665 408 1648
Fragments arranged by size:
1648 758 665 641 408 220
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 223 223
Size: 4340
BsiEI CG_ry'CG
Cuts at: 104 1882 1928 3158 104
Size: 1778 46 1230 1286
Fragments arranged by size:
1778 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1016 1354 2132 2322 3182 1016
Size: 338 778 190 860 2174
Fragments arranged by size:
2174 860 778 338 190
BsmBI CGTCTCn'nnnn_
Cuts at: 349 1230 349
Size: 881 3459
Bsp1286I G_dGCh'C
Cuts at: 544 904 1016 1080 1241 1294 1354 1965 2058
Size: 360 112 64 161 53 60 611 93
Cuts at: 2058 2132 2322 2384 3182 544
Size: 74 190 62 798 1702
Fragments arranged by size:
1702 798 611 360 190 161 112 93
74 64 62 60 53
BspHI T'CATG_A
Cuts at: 831 2772 831
Size: 1941 2399
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1390 1604 1906 2287 1390
Size: 214 302 381 3443
Fragments arranged by size:
3443 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2632 2686 4170 4335 50
Size: 2582 54 1484 165 55
Fragments arranged by size:
2582 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2252 2252
Size: 4340
BsrFI r'CCGG_y
Cuts at: 680 2338 2519 680
Size: 1658 181 2501
Fragments arranged by size:
2501 1658 181
BssSI C'ACGA_G
Cuts at: 651 2611 3319 651
Size: 1960 708 1672
Fragments arranged by size:
1960 1672 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1717 1717
Size: 4340
BstBI TT'CG_AA
Cuts at: 2701 2701
Size: 4340
BstEII G'GTnAC_C
Cuts at: 210 210
Size: 4340
BstYI r'GATC_y
Cuts at: 1 1365 1832 1855 2190 2436 2742 2754 2840
Size: 1364 467 23 335 246 306 12 86
Cuts at: 2840 3892 1
Size: 1052 449
Fragments arranged by size:
1364 1052 467 449 335 306 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 1294 107
Size: 1187 3153
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2290 2471 2634 2290
Size: 181 163 3996
Fragments arranged by size:
3996 181 163
BtsI GCAGTG_nn'
Cuts at: 138 147 938 1583 138
Size: 9 791 645 2895
Fragments arranged by size:
2895 791 645 9
DraI TTT'AAA
Cuts at: 1339 1339
Size: 4340
DraIII CAC_nnn'GTG
Cuts at: 408 408
Size: 4340
DrdI GACnn_nn'nnGTC
Cuts at: 2046 3390 2046
Size: 1344 2996
EaeI y'GGCC_r
Cuts at: 1925 2099 2490 2517 3862 1925
Size: 174 391 27 1345 2403
Fragments arranged by size:
2403 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1925 1925
Size: 4340
EarI CTCTTCn'nnn_
Cuts at: 451 1033 2363 2573 451
Size: 582 1330 210 2218
Fragments arranged by size:
2218 1330 582 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 789 2548 3290 3436 4150 789
Size: 1759 742 146 714 979
Fragments arranged by size:
1759 979 742 714 146
EcoICRI GAG'CTC
Cuts at: 1352 1352
Size: 4340
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 208 960 1057 2165 2597 2950 3612 208
Size: 752 97 1108 432 353 662 936
Fragments arranged by size:
1108 936 752 662 432 353 97
EcoO109I rG'GnC_Cy
Cuts at: 1479 1479
Size: 4340
EcoRI G'AATT_C
Cuts at: 427 613 1344 427
Size: 186 731 3423
Fragments arranged by size:
3423 731 186
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2359 2359
Size: 4340
FspI TGC'GCA
Cuts at: 2121 2121
Size: 4340
HaeII r_GCGC'y
Cuts at: 1774 2022 3252 4023 1774
Size: 248 1230 771 2091
Fragments arranged by size:
2091 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2182 2212 2214 2244 2182
Size: 30 2 30 4278
Fragments arranged by size:
4278 30 30 2
HincII GTy'rAC
Cuts at: 1233 1379 1233
Size: 146 4194
KasI G'GCGC_C
Cuts at: 1770 2018 1770
Size: 248 4092
KpnI G_GTAC'C
Cuts at: 1360 1360
Size: 4340
MluI A'CGCG_T
Cuts at: 3773 3773
Size: 4340
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3099 3283 3099
Size: 184 4156
MscI TGG'CCA
Cuts at: 2101 2101
Size: 4340
MslI CAynn'nnrTG
Cuts at: 410 601 2329 2456 410
Size: 191 1728 127 2294
Fragments arranged by size:
2294 1728 191 127
MspA1I CmG'CkG
Cuts at: 158 956 968 1451 1765 2125 2909 3154 158
Size: 798 12 483 314 360 784 245 1344
Fragments arranged by size:
1344 798 784 483 360 314 245 12
NaeI GCC'GGC
Cuts at: 2521 2521
Size: 4340
NarI GG'CG_CC
Cuts at: 1771 2019 1771
Size: 248 4092
NcoI C'CATG_G
Cuts at: 107 107
Size: 4340
NgoMIV G'CCGG_C
Cuts at: 2519 2519
Size: 4340
NspI r_CATG'y
Cuts at: 712 1255 1393 1893 3496 712
Size: 543 138 500 1603 1556
Fragments arranged by size:
1603 1556 543 500 138
PasI CC'CwG_GG
Cuts at: 566 566
Size: 4340
PciI A'CATG_T
Cuts at: 3492 3492
Size: 4340
PfoI T'CCnGG_A
Cuts at: 617 617
Size: 4340
PmeI GTTT'AAAC
Cuts at: 1339 1339
Size: 4340
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2786 2818 2786
Size: 32 4308
PshAI GACnn'nnGTC
Cuts at: 1303 1303
Size: 4340
PspOMI G'GGCC_C
Cuts at: 1290 1290
Size: 4340
PstI C_TGCA'G
Cuts at: 563 933 1191 1387 2072 563
Size: 370 258 196 685 2831
Fragments arranged by size:
2831 685 370 258 196
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4340
PvuII CAG'CTG
Cuts at: 956 1765 2125 956
Size: 809 360 3171
Fragments arranged by size:
3171 809 360
RsrII CG'GwC_CG
Cuts at: 1265 2535 1265
Size: 1270 3070
SacI G_AGCT'C
Cuts at: 1354 1354
Size: 4340
SalI G'TCGA_C
Cuts at: 1377 1377
Size: 4340
SapI GCTCTTCn'nnn_
Cuts at: 2363 2573 2363
Size: 210 4130
SbfI CC_TGCA'GG
Cuts at: 1387 1387
Size: 4340
ScaI AGT'ACT
Cuts at: 3857 3857
Size: 4340
SfcI C'TryA_G
Cuts at: 33 559 929 1169 1187 1383 2068 3036 3227
Size: 526 370 240 18 196 685 968 191
Cuts at: 3227 33
Size: 1146
Fragments arranged by size:
1146 968 685 526 370 240 196 191
18
SfoI GGC'GCC
Cuts at: 1772 2020 1772
Size: 248 4092
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4340
SmaI CCC'GGG
Cuts at: 1074 1362 1074
Size: 288 4052
SmlI C'TyrA_G
Cuts at: 277 1495 2410 3124 3386 277
Size: 1218 915 714 262 1231
Fragments arranged by size:
1231 1218 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4340
SphI G_CATG'C
Cuts at: 1393 1893 1393
Size: 500 3840
StuI AGG'CCT
Cuts at: 502 502
Size: 4340
StyI C'CwwG_G
Cuts at: 107 1474 1828 107
Size: 1367 354 2619
Fragments arranged by size:
2619 1367 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 740 1923 2056 2515 2724 740
Size: 1183 133 459 209 2356
Fragments arranged by size:
2356 1183 459 209 133
TatI w'GTAC_w
Cuts at: 3855 3855
Size: 4340
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1315 1347 3857 3889 1315
Size: 32 2510 32 1766
Fragments arranged by size:
2510 1766 32 32
Tth111I GACn'n_nGTC
Cuts at: 2137 2137
Size: 4340
XbaI T'CTAG_A
Cuts at: 1371 1371
Size: 4340
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1835 1835
Size: 4340
XmaI C'CCGG_G
Cuts at: 1072 1360 1072
Size: 288 4052
XmnI GAAnn'nnTTC
Cuts at: 1096 1096
Size: 4340
ZraI GAC'GTC
Cuts at: 268 268
Size: 4340
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PfoI PmeI PpiI
PshAI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AgeI AhdI AleI AlfI AloI
AscI AvrII BaeI BcgI BfrBI BglI BmgBI BmtI
BsiWI BsmI BspEI BsrGI BssHII BstXI BstZ17I Bsu36I
ClaI CspCI EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PflMI
PmlI PpuMI PsiI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI