Restriction map for
pF1KB8665
(Circular) (Six-base) MAPSORT of: pF1KB8665.seq Check: 456 from: 1 to: 4610
pF1KB8665 4610 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1113 1113
Size: 4610
AccI GT'mk_AC
Cuts at: 898 1648 898
Size: 750 3860
Acc65I G'GTAC_C
Cuts at: 1181 1626 1181
Size: 445 4165
AclI AA'CG_TT
Cuts at: 4450 4450
Size: 4610
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 323 1087 2435 2867 3220 3882 323
Size: 764 1348 432 353 662 1051
Fragments arranged by size:
1348 1051 764 662 432 353
AflIII A'CryG_T
Cuts at: 1962 3762 4043 1962
Size: 1800 281 2529
Fragments arranged by size:
2529 1800 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 871 871
Size: 4610
AlwNI CAG_nnn'CTG
Cuts at: 642 954 1485 1915 3353 642
Size: 312 531 430 1438 1899
Fragments arranged by size:
1899 1438 531 430 312
ApaI G_GGCC'C
Cuts at: 870 870
Size: 4610
ApaLI G'TGCA_C
Cuts at: 1002 3448 1002
Size: 2446 2164
ApoI r'AATT_y
Cuts at: 1087 1614 3963 4219 1087
Size: 527 2349 256 1478
Fragments arranged by size:
2349 1478 527 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4610
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4610
AvaI C'yCGr_G
Cuts at: 862 1443 1630 862
Size: 581 187 3842
Fragments arranged by size:
3842 581 187
BamHI G'GATC_C
Cuts at: 1635 1635
Size: 4610
BanI G'GyrC_C
Cuts at: 837 1181 1626 2040 2288 2323 837
Size: 344 445 414 248 35 3124
Fragments arranged by size:
3124 445 414 344 248 35
BanII G_rGCy'C
Cuts at: 128 870 1624 2654 128
Size: 742 754 1030 2084
Fragments arranged by size:
2084 1030 754 742
BbeI G_GCGC'C
Cuts at: 2044 2292 2044
Size: 248 4362
BbvCI CC'TCA_GC
Cuts at: 1526 1526
Size: 4610
BciVI GTATCCnnnnn_n'
Cuts at: 2508 3564 4610 2508
Size: 1056 1046 2508
Fragments arranged by size:
2508 1056 1046
BclI T'GATC_A
Cuts at: 2130 2130
Size: 4610
BglI GCCn_nnn'nGGC
Cuts at: 1304 1357 1304
Size: 53 4557
BglII A'GATC_T
Cuts at: 2125 4162 2125
Size: 2037 2573
BlpI GC'TnA_GC
Cuts at: 1722 1722
Size: 4610
Bme1580I G_kGCm'C
Cuts at: 842 870 1006 1450 2235 2328 3452 842
Size: 28 136 444 785 93 1124 2000
Fragments arranged by size:
2000 1124 785 444 136 93 28
BmrI ACTGGGnnnn_n'
Cuts at: 1822 1903 2238 4382 1822
Size: 81 335 2144 2050
Fragments arranged by size:
2144 2050 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2977 2977
Size: 4610
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 502 679 688 712 1440 1462 1507 1533 502
Size: 177 9 24 728 22 45 26 3579
Fragments arranged by size:
3579 728 177 45 26 24 22 9
Bpu10I CC'TnA_GC
Cuts at: 1526 1526
Size: 4610
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1552 1786 2665 3379 3677 1552
Size: 234 879 714 298 2485
Fragments arranged by size:
2485 879 714 298 234
BsaI GGTCTCn'nnnn_
Cuts at: 4314 4314
Size: 4610
BsaAI yAC'GTr
Cuts at: 1963 1963
Size: 4610
BsaBI GATnn'nnATC
Cuts at: 6 2124 4161 6
Size: 2118 2037 455
Fragments arranged by size:
2118 2037 455
BsaHI Gr'CG_yC
Cuts at: 1265 2041 2289 2991 4487 1265
Size: 776 248 702 1496 1388
Fragments arranged by size:
1496 1388 776 702 248
BsaWI w'CCGG_w
Cuts at: 960 1220 1799 2320 2998 3409 3556 3830 960
Size: 260 579 521 678 411 147 274 1740
Fragments arranged by size:
1740 678 579 521 411 274 260 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 172 205 232 325 664 667 685 1273 1431
Size: 33 27 93 339 3 18 588 158
Cuts at: 1431 172
Size: 3351
Fragments arranged by size:
3351 588 339 158 93 33 27 18
3
BseYI C'CCAG_C
Cuts at: 531 869 1203 2035 2793 3458 3866 531
Size: 338 334 832 758 665 408 1275
Fragments arranged by size:
1275 832 758 665 408 338 334
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 232 967 232
Size: 735 3875
BsiEI CG_ry'CG
Cuts at: 104 203 791 1176 1437 2152 2198 3428 104
Size: 99 588 385 261 715 46 1230 1286
Fragments arranged by size:
1286 1230 715 588 385 261 99 46
BsiHKAI G_wGCw'C
Cuts at: 128 1006 1042 1624 2402 2592 3452 128
Size: 878 36 582 778 190 860 1286
Fragments arranged by size:
1286 878 860 778 582 190 36
Bsp1286I G_dGCh'C
Cuts at: 128 842 870 1006 1042 1450 1624 2235 2328
Size: 714 28 136 36 408 174 611 93
Cuts at: 2328 2402 2592 2654 3452 128
Size: 74 190 62 798 1286
Fragments arranged by size:
1286 798 714 611 408 190 174 136
93 74 62 36 28
BspEI T'CCGG_A
Cuts at: 1220 1220
Size: 4610
BspHI T'CATG_A
Cuts at: 3042 3042
Size: 4610
BspMI ACCTGCnnnn'nnnn_
Cuts at: 578 1113 1169 1660 1874 2176 2557 578
Size: 535 56 491 214 302 381 2631
Fragments arranged by size:
2631 535 491 381 302 214 56
BsrBI CCG'CTC
Cuts at: 50 663 1200 1439 2902 2956 4440 4605 50
Size: 613 537 239 1463 54 1484 165 55
Fragments arranged by size:
1484 1463 613 537 239 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2522 2522
Size: 4610
BsrFI r'CCGG_y
Cuts at: 811 919 2608 2789 811
Size: 108 1689 181 2632
Fragments arranged by size:
2632 1689 181 108
BssSI C'ACGA_G
Cuts at: 1033 2881 3589 1033
Size: 1848 708 2054
Fragments arranged by size:
2054 1848 708
BstAPI GCAn_nnn'nTGC
Cuts at: 954 1987 954
Size: 1033 3577
BstBI TT'CG_AA
Cuts at: 2971 2971
Size: 4610
BstXI CCAn_nnnn'nTGG
Cuts at: 114 504 600 801 114
Size: 390 96 201 3923
Fragments arranged by size:
3923 390 201 96
BstYI r'GATC_y
Cuts at: 1 231 578 963 1217 1364 1635 2102 2125
Size: 230 347 385 254 147 271 467 23
Cuts at: 2125 2460 2706 3012 3024 3110 4162 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
1052 467 449 385 347 335 306 271
254 246 230 147 86 23 12
BtgI C'CryG_G
Cuts at: 407 785 407
Size: 378 4232
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 243 735 2560 2741 2904 243
Size: 492 1825 181 163 1949
Fragments arranged by size:
1949 1825 492 181 163
BtsI GCAGTG_nn'
Cuts at: 1323 1853 1323
Size: 530 4080
DraI TTT'AAA
Cuts at: 1609 1609
Size: 4610
DrdI GACnn_nn'nnGTC
Cuts at: 2316 3660 2316
Size: 1344 3266
EaeI y'GGCC_r
Cuts at: 788 809 992 1173 2195 2369 2760 2787 4132
Size: 21 183 181 1022 174 391 27 1345
Cuts at: 4132 788
Size: 1266
Fragments arranged by size:
1345 1266 1022 391 183 181 174 27
21
EagI C'GGCC_G
Cuts at: 788 1173 2195 788
Size: 385 1022 3203
Fragments arranged by size:
3203 1022 385
EarI CTCTTCn'nnn_
Cuts at: 137 149 182 1579 2633 2843 137
Size: 12 33 1397 1054 210 1904
Fragments arranged by size:
1904 1397 1054 210 33 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 987 2818 3560 3706 4420 987
Size: 1831 742 146 714 1177
Fragments arranged by size:
1831 1177 742 714 146
EcoICRI GAG'CTC
Cuts at: 126 1622 126
Size: 1496 3114
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 323 502 679 688 712 1087 1440 1462 1507
Size: 179 177 9 24 375 353 22 45
Cuts at: 1507 1533 2435 2867 3220 3882 323
Size: 26 902 432 353 662 1051
Fragments arranged by size:
1051 902 662 432 375 353 353 179
177 45 26 24 22 9
EcoNI CCTnn'n_nnAGG
Cuts at: 832 832
Size: 4610
EcoO109I rG'GnC_Cy
Cuts at: 866 1278 1482 1749 866
Size: 412 204 267 3727
Fragments arranged by size:
3727 412 267 204
EcoRI G'AATT_C
Cuts at: 1614 1614
Size: 4610
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2629 2629
Size: 4610
FspI TGC'GCA
Cuts at: 2391 2391
Size: 4610
HaeII r_GCGC'y
Cuts at: 691 2044 2292 3522 4293 691
Size: 1353 248 1230 771 1008
Fragments arranged by size:
1353 1230 1008 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 445 477 1426 1458 2452 2482 2484 2514 445
Size: 32 949 32 994 30 2 30 2541
Fragments arranged by size:
2541 994 949 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1649 1649
Size: 4610
HindIII A'AGCT_T
Cuts at: 335 335
Size: 4610
KasI G'GCGC_C
Cuts at: 2040 2288 2040
Size: 248 4362
KpnI G_GTAC'C
Cuts at: 1185 1630 1185
Size: 445 4165
MluI A'CGCG_T
Cuts at: 4043 4043
Size: 4610
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 626 3369 3553 626
Size: 2743 184 1683
Fragments arranged by size:
2743 1683 184
MscI TGG'CCA
Cuts at: 2371 2371
Size: 4610
MslI CAynn'nnrTG
Cuts at: 598 805 2599 2726 598
Size: 207 1794 127 2482
Fragments arranged by size:
2482 1794 207 127
MspA1I CmG'CkG
Cuts at: 228 642 1134 1158 1383 1721 2035 2395 3179
Size: 414 492 24 225 338 314 360 784
Cuts at: 3179 3424 228
Size: 245 1414
Fragments arranged by size:
1414 784 492 414 360 338 314 245
225 24
NaeI GCC'GGC
Cuts at: 813 2791 813
Size: 1978 2632
NarI GG'CG_CC
Cuts at: 2041 2289 2041
Size: 248 4362
NgoMIV G'CCGG_C
Cuts at: 811 2789 811
Size: 1978 2632
NotI GC'GGCC_GC
Cuts at: 1173 1173
Size: 4610
NspI r_CATG'y
Cuts at: 1663 2163 3766 1663
Size: 500 1603 2507
Fragments arranged by size:
2507 1603 500
PasI CC'CwG_GG
Cuts at: 630 630
Size: 4610
PciI A'CATG_T
Cuts at: 3762 3762
Size: 4610
PflMI CCAn_nnn'nTGG
Cuts at: 269 1485 269
Size: 1216 3394
PfoI T'CCnGG_A
Cuts at: 449 736 449
Size: 287 4323
PmeI GTTT'AAAC
Cuts at: 1609 1609
Size: 4610
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3056 3088 3056
Size: 32 4578
PpuMI rG'GwC_Cy
Cuts at: 1278 1278
Size: 4610
PspOMI G'GGCC_C
Cuts at: 866 866
Size: 4610
PstI C_TGCA'G
Cuts at: 575 1382 1394 1657 2342 575
Size: 807 12 263 685 2843
Fragments arranged by size:
2843 807 685 263 12
PvuI CG_AT'CG
Cuts at: 104 203 104
Size: 99 4511
PvuII CAG'CTG
Cuts at: 228 642 2035 2395 228
Size: 414 1393 360 2443
Fragments arranged by size:
2443 1393 414 360
RsrII CG'GwC_CG
Cuts at: 2805 2805
Size: 4610
SacI G_AGCT'C
Cuts at: 128 1624 128
Size: 1496 3114
SalI G'TCGA_C
Cuts at: 1647 1647
Size: 4610
SapI GCTCTTCn'nnn_
Cuts at: 2633 2843 2633
Size: 210 4400
SbfI CC_TGCA'GG
Cuts at: 1657 1657
Size: 4610
ScaI AGT'ACT
Cuts at: 460 4127 460
Size: 3667 943
SfcI C'TryA_G
Cuts at: 33 571 1378 1390 1653 2338 3306 3497 33
Size: 538 807 12 263 685 968 191 1146
Fragments arranged by size:
1146 968 807 685 538 263 191 12
SfiI GGCCn_nnn'nGGCC
Cuts at: 1357 1357
Size: 4610
SfoI GGC'GCC
Cuts at: 2042 2290 2042
Size: 248 4362
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4610
SmaI CCC'GGG
Cuts at: 1632 1632
Size: 4610
SmlI C'TyrA_G
Cuts at: 1567 1765 2680 3394 3656 1567
Size: 198 915 714 262 2521
Fragments arranged by size:
2521 915 714 262 198
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4610
SphI G_CATG'C
Cuts at: 1663 2163 1663
Size: 500 4110
StuI AGG'CCT
Cuts at: 676 676
Size: 4610
StyI C'CwwG_G
Cuts at: 488 833 1355 1744 2098 488
Size: 345 522 389 354 3000
Fragments arranged by size:
3000 522 389 354 345
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 643 771 793 2193 2326 2785 2994 643
Size: 128 22 1400 133 459 209 2259
Fragments arranged by size:
2259 1400 459 209 133 128 22
TatI w'GTAC_w
Cuts at: 458 4125 458
Size: 3667 943
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4127 4159 4127
Size: 32 4578
Tth111I GACn'n_nGTC
Cuts at: 882 2407 882
Size: 1525 3085
XbaI T'CTAG_A
Cuts at: 1641 1641
Size: 4610
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1005 2105 1005
Size: 1100 3510
XmaI C'CCGG_G
Cuts at: 1630 1630
Size: 4610
XmnI GAAnn'nnTTC
Cuts at: 1543 1543
Size: 4610
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbvCI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfiI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AleI AloI AscI
AvrII BaeI BbsI BcgI BfrBI BmgBI BmtI BsaXI
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I
ClaI CspCI DraIII EcoRV FseI FspAI HpaI MfeI
NcoI NdeI NheI NruI NsiI PacI PmlI PshAI
PsiI PspXI PsrI SacII SanDI SexAI SgrAI SnaBI
SrfI SspI SwaI XhoI ZraI