Restriction map for
pF1KB8666
(Circular) (Six-base) MAPSORT of: pF1KB8666.seq Check: 5077 from: 1 to: 4199
pF1KB8666 4199 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AccI GT'mk_AC
Cuts at: 1237 1237
Size: 4199
Acc65I G'GTAC_C
Cuts at: 1215 1215
Size: 4199
AclI AA'CG_TT
Cuts at: 4039 4039
Size: 4199
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 550 877 2024 2456 2809 3471 550
Size: 327 1147 432 353 662 1278
Fragments arranged by size:
1278 1147 662 432 353 327
AfeI AGC'GCT
Cuts at: 454 454
Size: 4199
AflIII A'CryG_T
Cuts at: 1077 1551 3351 3632 1077
Size: 474 1800 281 1644
Fragments arranged by size:
1800 1644 474 281
AlwNI CAG_nnn'CTG
Cuts at: 528 963 1504 2942 528
Size: 435 541 1438 1785
Fragments arranged by size:
1785 1438 541 435
ApaI G_GGCC'C
Cuts at: 654 654
Size: 4199
ApaLI G'TGCA_C
Cuts at: 3037 3037
Size: 4199
ApoI r'AATT_y
Cuts at: 505 775 1203 3552 3808 505
Size: 270 428 2349 256 896
Fragments arranged by size:
2349 896 428 270 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4199
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4199
AvaI C'yCGr_G
Cuts at: 632 1219 632
Size: 587 3612
BamHI G'GATC_C
Cuts at: 1224 1224
Size: 4199
BanI G'GyrC_C
Cuts at: 1215 1629 1877 1912 1215
Size: 414 248 35 3502
Fragments arranged by size:
3502 414 248 35
BanII G_rGCy'C
Cuts at: 654 848 1213 2243 654
Size: 194 365 1030 2610
Fragments arranged by size:
2610 1030 365 194
BbeI G_GCGC'C
Cuts at: 1633 1881 1633
Size: 248 3951
BbsI GAAGACnn'nnnn_
Cuts at: 335 556 335
Size: 221 3978
BciVI GTATCCnnnnn_n'
Cuts at: 2097 3153 4199 2097
Size: 1056 1046 2097
Fragments arranged by size:
2097 1056 1046
BclI T'GATC_A
Cuts at: 1719 1719
Size: 4199
BglII A'GATC_T
Cuts at: 1714 3751 1714
Size: 2037 2162
BlpI GC'TnA_GC
Cuts at: 1311 1311
Size: 4199
Bme1580I G_kGCm'C
Cuts at: 319 654 1824 1917 3041 319
Size: 335 1170 93 1124 1477
Fragments arranged by size:
1477 1170 1124 335 93
BmrI ACTGGGnnnn_n'
Cuts at: 1411 1492 1827 3971 1411
Size: 81 335 2144 1639
Fragments arranged by size:
2144 1639 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1134 2566 1134
Size: 1432 2767
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 717 717
Size: 4199
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1375 2254 2968 3266 1375
Size: 879 714 298 2308
Fragments arranged by size:
2308 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 967 3903 967
Size: 2936 1263
BsaAI yAC'GTr
Cuts at: 1552 1552
Size: 4199
BsaBI GATnn'nnATC
Cuts at: 6 1713 3750 6
Size: 1707 2037 455
Fragments arranged by size:
2037 1707 455
BsaHI Gr'CG_yC
Cuts at: 1630 1878 2580 4076 1630
Size: 248 702 1496 1753
Fragments arranged by size:
1753 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1388 1909 2587 2998 3145 3419 1388
Size: 521 678 411 147 274 2168
Fragments arranged by size:
2168 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 690 720 732 762 970 1000 690
Size: 30 12 30 208 30 3889
Fragments arranged by size:
3889 208 30 30 30 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1126 1126
Size: 4199
BseYI C'CCAG_C
Cuts at: 1624 2382 3047 3455 1624
Size: 758 665 408 2368
Fragments arranged by size:
2368 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 976 1055 976
Size: 79 4120
BsiEI CG_ry'CG
Cuts at: 104 1741 1787 3017 104
Size: 1637 46 1230 1286
Fragments arranged by size:
1637 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 823 848 988 1213 1991 2181 3041 823
Size: 25 140 225 778 190 860 1981
Fragments arranged by size:
1981 860 778 225 190 140 25
Bsp1286I G_dGCh'C
Cuts at: 319 654 823 848 988 1213 1824 1917 1991
Size: 335 169 25 140 225 611 93 74
Cuts at: 1991 2181 2243 3041 319
Size: 190 62 798 1477
Fragments arranged by size:
1477 798 611 335 225 190 169 140
93 74 62 25
BspHI T'CATG_A
Cuts at: 2631 2631
Size: 4199
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1249 1463 1765 2146 1249
Size: 214 302 381 3302
Fragments arranged by size:
3302 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1163 2491 2545 4029 4194 50
Size: 1113 1328 54 1484 165 55
Fragments arranged by size:
1484 1328 1113 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2111 2111
Size: 4199
BsrFI r'CCGG_y
Cuts at: 2197 2378 2197
Size: 181 4018
BssSI C'ACGA_G
Cuts at: 979 2470 3178 979
Size: 1491 708 2000
Fragments arranged by size:
2000 1491 708
BstAPI GCAn_nnn'nTGC
Cuts at: 157 818 963 1576 157
Size: 661 145 613 2780
Fragments arranged by size:
2780 661 613 145
BstBI TT'CG_AA
Cuts at: 201 2560 201
Size: 2359 1840
BstXI CCAn_nnnn'nTGG
Cuts at: 996 1056 996
Size: 60 4139
BstYI r'GATC_y
Cuts at: 1 1224 1691 1714 2049 2295 2601 2613 2699
Size: 1223 467 23 335 246 306 12 86
Cuts at: 2699 3751 1
Size: 1052 449
Fragments arranged by size:
1223 1052 467 449 335 306 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 521 733 521
Size: 212 3987
BtgI C'CryG_G
Cuts at: 1121 1121
Size: 4199
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2149 2330 2493 2149
Size: 181 163 3855
Fragments arranged by size:
3855 181 163
BtsI GCAGTG_nn'
Cuts at: 1442 1442
Size: 4199
ClaI AT'CG_AT
Cuts at: 860 860
Size: 4199
DraI TTT'AAA
Cuts at: 1198 1198
Size: 4199
DrdI GACnn_nn'nnGTC
Cuts at: 1905 3249 1905
Size: 1344 2855
EaeI y'GGCC_r
Cuts at: 872 915 1784 1958 2349 2376 3721 872
Size: 43 869 174 391 27 1345 1350
Fragments arranged by size:
1350 1345 869 391 174 43 27
EagI C'GGCC_G
Cuts at: 1784 1784
Size: 4199
EarI CTCTTCn'nnn_
Cuts at: 836 926 2222 2432 836
Size: 90 1296 210 2603
Fragments arranged by size:
2603 1296 210 90
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2407 3149 3295 4009 2407
Size: 742 146 714 2597
Fragments arranged by size:
2597 742 714 146
EcoICRI GAG'CTC
Cuts at: 846 1211 846
Size: 365 3834
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 550 717 877 2024 2456 2809 3471 550
Size: 167 160 1147 432 353 662 1278
Fragments arranged by size:
1278 1147 662 432 353 167 160
EcoO109I rG'GnC_Cy
Cuts at: 650 1338 650
Size: 688 3511
EcoRI G'AATT_C
Cuts at: 505 1203 505
Size: 698 3501
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 580 2218 580
Size: 1638 2561
FspI TGC'GCA
Cuts at: 1980 1980
Size: 4199
HaeII r_GCGC'y
Cuts at: 456 1633 1881 3111 3882 456
Size: 1177 248 1230 771 773
Fragments arranged by size:
1230 1177 773 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 241 273 2041 2071 2073 2103 241
Size: 32 1768 30 2 30 2337
Fragments arranged by size:
2337 1768 32 30 30 2
HincII GTy'rAC
Cuts at: 374 1238 374
Size: 864 3335
KasI G'GCGC_C
Cuts at: 1629 1877 1629
Size: 248 3951
KpnI G_GTAC'C
Cuts at: 1219 1219
Size: 4199
MluI A'CGCG_T
Cuts at: 3632 3632
Size: 4199
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 881 2958 3142 881
Size: 2077 184 1938
Fragments arranged by size:
2077 1938 184
MscI TGG'CCA
Cuts at: 874 917 1960 874
Size: 43 1043 3113
Fragments arranged by size:
3113 1043 43
MslI CAynn'nnrTG
Cuts at: 2188 2315 2188
Size: 127 4072
MspA1I CmG'CkG
Cuts at: 1012 1310 1624 1984 2768 3013 1012
Size: 298 314 360 784 245 2198
Fragments arranged by size:
2198 784 360 314 298 245
NaeI GCC'GGC
Cuts at: 2380 2380
Size: 4199
NarI GG'CG_CC
Cuts at: 1630 1878 1630
Size: 248 3951
NgoMIV G'CCGG_C
Cuts at: 2378 2378
Size: 4199
NspI r_CATG'y
Cuts at: 563 1081 1252 1752 3355 563
Size: 518 171 500 1603 1407
Fragments arranged by size:
1603 1407 518 500 171
PciI A'CATG_T
Cuts at: 1077 3351 1077
Size: 2274 1925
PmeI GTTT'AAAC
Cuts at: 1198 1198
Size: 4199
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2645 2677 2645
Size: 32 4167
PspOMI G'GGCC_C
Cuts at: 650 650
Size: 4199
PstI C_TGCA'G
Cuts at: 1246 1931 1246
Size: 685 3514
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4199
PvuII CAG'CTG
Cuts at: 1624 1984 1624
Size: 360 3839
RsrII CG'GwC_CG
Cuts at: 2394 2394
Size: 4199
SacI G_AGCT'C
Cuts at: 848 1213 848
Size: 365 3834
SalI G'TCGA_C
Cuts at: 1236 1236
Size: 4199
SapI GCTCTTCn'nnn_
Cuts at: 836 2222 2432 836
Size: 1386 210 2603
Fragments arranged by size:
2603 1386 210
SbfI CC_TGCA'GG
Cuts at: 1246 1246
Size: 4199
ScaI AGT'ACT
Cuts at: 3716 3716
Size: 4199
SfcI C'TryA_G
Cuts at: 33 609 1242 1927 2895 3086 33
Size: 576 633 685 968 191 1146
Fragments arranged by size:
1146 968 685 633 576 191
SfoI GGC'GCC
Cuts at: 1631 1879 1631
Size: 248 3951
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4199
SmaI CCC'GGG
Cuts at: 1221 1221
Size: 4199
SmlI C'TyrA_G
Cuts at: 1354 2269 2983 3245 1354
Size: 915 714 262 2308
Fragments arranged by size:
2308 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4199
SphI G_CATG'C
Cuts at: 1252 1752 1252
Size: 500 3699
StuI AGG'CCT
Cuts at: 1134 1134
Size: 4199
StyI C'CwwG_G
Cuts at: 1333 1687 1333
Size: 354 3845
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1021 1782 1915 2374 2583 1021
Size: 761 133 459 209 2637
Fragments arranged by size:
2637 761 459 209 133
TatI w'GTAC_w
Cuts at: 237 3714 237
Size: 3477 722
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 690 722 970 1002 1067 1099 3716 3748 690
Size: 32 248 32 65 32 2617 32 1141
Fragments arranged by size:
2617 1141 248 65 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1996 1996
Size: 4199
XbaI T'CTAG_A
Cuts at: 1230 1230
Size: 4199
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1694 1694
Size: 4199
XmaI C'CCGG_G
Cuts at: 1219 1219
Size: 4199
XmnI GAAnn'nnTTC
Cuts at: 207 347 207
Size: 140 4059
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PciI PmeI PpiI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AgeI AhdI AleI AlfI AloI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BsiWI BsmI BsmBI BspEI BsrGI BssHII
BstEII BstZ17I CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NcoI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PfoI PmlI PpuMI PshAI
PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI ZraI