Restriction map for
pF1KB8667
(Circular) (Six-base) MAPSORT of: pF1KB8667.seq Check: 2807 from: 1 to: 4385
pF1KB8667 4385 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AccI GT'mk_AC
Cuts at: 545 1423 545
Size: 878 3507
Acc65I G'GTAC_C
Cuts at: 1401 1401
Size: 4385
AclI AA'CG_TT
Cuts at: 4225 4225
Size: 4385
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 775 2210 2642 2995 3657 775
Size: 1435 432 353 662 1503
Fragments arranged by size:
1503 1435 662 432 353
AflIII A'CryG_T
Cuts at: 1737 3537 3818 1737
Size: 1800 281 2304
Fragments arranged by size:
2304 1800 281
AlwNI CAG_nnn'CTG
Cuts at: 291 1690 3128 291
Size: 1399 1438 1548
Fragments arranged by size:
1548 1438 1399
ApaI G_GGCC'C
Cuts at: 1336 1336
Size: 4385
ApaLI G'TGCA_C
Cuts at: 3223 3223
Size: 4385
ApoI r'AATT_y
Cuts at: 529 875 1389 3738 3994 529
Size: 346 514 2349 256 920
Fragments arranged by size:
2349 920 514 346 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4385
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4385
AvaI C'yCGr_G
Cuts at: 1405 1405
Size: 4385
BamHI G'GATC_C
Cuts at: 468 832 1410 468
Size: 364 578 3443
Fragments arranged by size:
3443 578 364
BanI G'GyrC_C
Cuts at: 1401 1815 2063 2098 1401
Size: 414 248 35 3688
Fragments arranged by size:
3688 414 248 35
BanII G_rGCy'C
Cuts at: 308 1336 1399 2429 308
Size: 1028 63 1030 2264
Fragments arranged by size:
2264 1030 1028 63
BbeI G_GCGC'C
Cuts at: 1819 2067 1819
Size: 248 4137
BbsI GAAGACnn'nnnn_
Cuts at: 970 970
Size: 4385
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1150 1184 1150
Size: 34 4351
BciVI GTATCCnnnnn_n'
Cuts at: 2283 3339 4385 2283
Size: 1056 1046 2283
Fragments arranged by size:
2283 1056 1046
BclI T'GATC_A
Cuts at: 1905 1905
Size: 4385
BfrBI ATG'CAT
Cuts at: 664 664
Size: 4385
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 4385
BglII A'GATC_T
Cuts at: 1900 3937 1900
Size: 2037 2348
BlpI GC'TnA_GC
Cuts at: 1497 1497
Size: 4385
Bme1580I G_kGCm'C
Cuts at: 262 1336 2010 2103 3227 262
Size: 1074 674 93 1124 1420
Fragments arranged by size:
1420 1124 1074 674 93
BmrI ACTGGGnnnn_n'
Cuts at: 158 1597 1678 2013 4157 158
Size: 1439 81 335 2144 386
Fragments arranged by size:
2144 1439 386 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1007 2752 1007
Size: 1745 2640
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1250 1250
Size: 4385
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1278 1561 2440 3154 3452 1278
Size: 283 879 714 298 2211
Fragments arranged by size:
2211 879 714 298 283
BsaI GGTCTCn'nnnn_
Cuts at: 4089 4089
Size: 4385
BsaAI yAC'GTr
Cuts at: 1738 1738
Size: 4385
BsaBI GATnn'nnATC
Cuts at: 6 1899 3936 6
Size: 1893 2037 455
Fragments arranged by size:
2037 1893 455
BsaHI Gr'CG_yC
Cuts at: 1816 2064 2766 4262 1816
Size: 248 702 1496 1939
Fragments arranged by size:
1939 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1574 2095 2773 3184 3331 3605 1574
Size: 521 678 411 147 274 2354
Fragments arranged by size:
2354 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 213 382 213
Size: 169 4216
BseYI C'CCAG_C
Cuts at: 309 1329 1810 2568 3233 3641 309
Size: 1020 481 758 665 408 1053
Fragments arranged by size:
1053 1020 758 665 481 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 941 941
Size: 4385
BsiEI CG_ry'CG
Cuts at: 104 268 1927 1973 3203 104
Size: 164 1659 46 1230 1286
Fragments arranged by size:
1659 1286 1230 164 46
BsiHKAI G_wGCw'C
Cuts at: 1399 2177 2367 3227 1399
Size: 778 190 860 2557
Fragments arranged by size:
2557 860 778 190
BsmI GAATG_Cn'
Cuts at: 680 680
Size: 4385
BsmBI CGTCTCn'nnnn_
Cuts at: 899 899
Size: 4385
Bsp1286I G_dGCh'C
Cuts at: 262 308 1336 1399 2010 2103 2177 2367 2429
Size: 46 1028 63 611 93 74 190 62
Cuts at: 2429 3227 262
Size: 798 1420
Fragments arranged by size:
1420 1028 798 611 190 93 74 63
62 46
BspHI T'CATG_A
Cuts at: 2817 2817
Size: 4385
BspMI ACCTGCnnnn'nnnn_
Cuts at: 133 1435 1649 1951 2332 133
Size: 1302 214 302 381 2186
Fragments arranged by size:
2186 1302 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1292 2677 2731 4215 4380 50
Size: 1242 1385 54 1484 165 55
Fragments arranged by size:
1484 1385 1242 165 55 54
BsrDI GCAATG_nn'
Cuts at: 251 2297 251
Size: 2046 2339
BsrFI r'CCGG_y
Cuts at: 2383 2564 2383
Size: 181 4204
BssSI C'ACGA_G
Cuts at: 2656 3364 2656
Size: 708 3677
BstAPI GCAn_nnn'nTGC
Cuts at: 1762 1762
Size: 4385
BstBI TT'CG_AA
Cuts at: 2746 2746
Size: 4385
BstXI CCAn_nnnn'nTGG
Cuts at: 1329 1329
Size: 4385
BstYI r'GATC_y
Cuts at: 1 468 832 1305 1410 1877 1900 2235 2481
Size: 467 364 473 105 467 23 335 246
Cuts at: 2481 2787 2799 2885 3937 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1052 473 467 467 449 364 335 306
246 105 86 23 12
BstZ17I GTA'TAC
Cuts at: 546 546
Size: 4385
BtgI C'CryG_G
Cuts at: 107 972 107
Size: 865 3520
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2335 2516 2679 2335
Size: 181 163 4041
Fragments arranged by size:
4041 181 163
BtsI GCAGTG_nn'
Cuts at: 122 164 1628 122
Size: 42 1464 2879
Fragments arranged by size:
2879 1464 42
DraI TTT'AAA
Cuts at: 1221 1384 1221
Size: 163 4222
DrdI GACnn_nn'nnGTC
Cuts at: 2091 3435 2091
Size: 1344 3041
EaeI y'GGCC_r
Cuts at: 1352 1970 2144 2535 2562 3907 1352
Size: 618 174 391 27 1345 1830
Fragments arranged by size:
1830 1345 618 391 174 27
EagI C'GGCC_G
Cuts at: 1970 1970
Size: 4385
EarI CTCTTCn'nnn_
Cuts at: 406 2408 2618 406
Size: 2002 210 2173
Fragments arranged by size:
2173 2002 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1308 2593 3335 3481 4195 1308
Size: 1285 742 146 714 1498
Fragments arranged by size:
1498 1285 742 714 146
EcoICRI GAG'CTC
Cuts at: 1397 1397
Size: 4385
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 775 1250 2210 2642 2995 3657 775
Size: 475 960 432 353 662 1503
Fragments arranged by size:
1503 960 662 475 432 353
EcoO109I rG'GnC_Cy
Cuts at: 205 1524 205
Size: 1319 3066
EcoRI G'AATT_C
Cuts at: 875 1389 875
Size: 514 3871
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2404 2404
Size: 4385
FspI TGC'GCA
Cuts at: 2166 2166
Size: 4385
HaeII r_GCGC'y
Cuts at: 1819 2067 3297 4068 1819
Size: 248 1230 771 2136
Fragments arranged by size:
2136 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 189 221 718 750 2227 2257 2259 2289 189
Size: 32 497 32 1477 30 2 30 2285
Fragments arranged by size:
2285 1477 497 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1424 1424
Size: 4385
KasI G'GCGC_C
Cuts at: 1815 2063 1815
Size: 248 4137
KpnI G_GTAC'C
Cuts at: 1405 1405
Size: 4385
MluI A'CGCG_T
Cuts at: 3818 3818
Size: 4385
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 327 3144 3328 327
Size: 2817 184 1384
Fragments arranged by size:
2817 1384 184
MscI TGG'CCA
Cuts at: 2146 2146
Size: 4385
MslI CAynn'nnrTG
Cuts at: 656 897 1012 2374 2501 656
Size: 241 115 1362 127 2540
Fragments arranged by size:
2540 1362 241 127 115
MspA1I CmG'CkG
Cuts at: 233 288 1496 1810 2170 2954 3199 233
Size: 55 1208 314 360 784 245 1419
Fragments arranged by size:
1419 1208 784 360 314 245 55
NaeI GCC'GGC
Cuts at: 2566 2566
Size: 4385
NarI GG'CG_CC
Cuts at: 1816 2064 1816
Size: 248 4137
NcoI C'CATG_G
Cuts at: 107 107
Size: 4385
NgoMIV G'CCGG_C
Cuts at: 2564 2564
Size: 4385
NsiI A_TGCA'T
Cuts at: 666 666
Size: 4385
NspI r_CATG'y
Cuts at: 1438 1938 3541 1438
Size: 500 1603 2282
Fragments arranged by size:
2282 1603 500
PciI A'CATG_T
Cuts at: 3537 3537
Size: 4385
PflMI CCAn_nnn'nTGG
Cuts at: 1342 1342
Size: 4385
PmeI GTTT'AAAC
Cuts at: 1384 1384
Size: 4385
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2831 2863 2831
Size: 32 4353
PpuMI rG'GwC_Cy
Cuts at: 205 205
Size: 4385
PshAI GACnn'nnGTC
Cuts at: 246 246
Size: 4385
PspOMI G'GGCC_C
Cuts at: 1332 1332
Size: 4385
PstI C_TGCA'G
Cuts at: 159 1432 2117 159
Size: 1273 685 2427
Fragments arranged by size:
2427 1273 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4385
PvuII CAG'CTG
Cuts at: 288 1810 2170 288
Size: 1522 360 2503
Fragments arranged by size:
2503 1522 360
RsrII CG'GwC_CG
Cuts at: 2580 2580
Size: 4385
SacI G_AGCT'C
Cuts at: 1399 1399
Size: 4385
SalI G'TCGA_C
Cuts at: 1422 1422
Size: 4385
SapI GCTCTTCn'nnn_
Cuts at: 406 2408 2618 406
Size: 2002 210 2173
Fragments arranged by size:
2173 2002 210
SbfI CC_TGCA'GG
Cuts at: 1432 1432
Size: 4385
ScaI AGT'ACT
Cuts at: 147 3902 147
Size: 3755 630
SfcI C'TryA_G
Cuts at: 33 155 1428 2113 3081 3272 33
Size: 122 1273 685 968 191 1146
Fragments arranged by size:
1273 1146 968 685 191 122
SfoI GGC'GCC
Cuts at: 1817 2065 1817
Size: 248 4137
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4385
SmaI CCC'GGG
Cuts at: 1407 1407
Size: 4385
SmlI C'TyrA_G
Cuts at: 1293 1540 2455 3169 3431 1293
Size: 247 915 714 262 2247
Fragments arranged by size:
2247 915 714 262 247
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4385
SphI G_CATG'C
Cuts at: 1438 1938 1438
Size: 500 3885
SspI AAT'ATT
Cuts at: 384 573 384
Size: 189 4196
StyI C'CwwG_G
Cuts at: 107 1519 1873 107
Size: 1412 354 2619
Fragments arranged by size:
2619 1412 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1968 2101 2560 2769 1968
Size: 133 459 209 3584
Fragments arranged by size:
3584 459 209 133
TatI w'GTAC_w
Cuts at: 145 3900 145
Size: 3755 630
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1013 1045 3902 3934 1013
Size: 32 2857 32 1464
Fragments arranged by size:
2857 1464 32 32
Tth111I GACn'n_nGTC
Cuts at: 2182 2182
Size: 4385
XbaI T'CTAG_A
Cuts at: 1416 1416
Size: 4385
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 328 1880 114
Size: 214 1552 2619
Fragments arranged by size:
2619 1552 214
XmaI C'CCGG_G
Cuts at: 1405 1405
Size: 4385
XmnI GAAnn'nnTTC
Cuts at: 1237 1237
Size: 4385
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI BstZ17I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NsiI NspI PciI PflMI PmeI PpiI
PpuMI PshAI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
AloI AscI AvrII BaeI BbvCI BmgBI BmtI Bpu10I
BsaXI BsiWI BspEI BsrGI BssHII BstEII Bsu36I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI PacI PasI PfoI
PmlI PsiI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI StuI SwaI XhoI ZraI