Restriction map for
pF1KB8668
(Circular) (Six-base) MAPSORT of: pF1KB8668.seq Check: 9383 from: 1 to: 4502
pF1KB8668 4502 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AccI GT'mk_AC
Cuts at: 1162 1540 1162
Size: 378 4124
Acc65I G'GTAC_C
Cuts at: 1518 1518
Size: 4502
AclI AA'CG_TT
Cuts at: 4342 4342
Size: 4502
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 503 2327 2759 3112 3774 503
Size: 1824 432 353 662 1231
Fragments arranged by size:
1824 1231 662 432 353
AflIII A'CryG_T
Cuts at: 313 1854 3654 3935 313
Size: 1541 1800 281 880
Fragments arranged by size:
1800 1541 880 281
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 893 925 893
Size: 32 4470
AlwNI CAG_nnn'CTG
Cuts at: 1807 3245 1807
Size: 1438 3064
ApaLI G'TGCA_C
Cuts at: 816 3340 816
Size: 2524 1978
ApoI r'AATT_y
Cuts at: 496 1506 3855 4111 496
Size: 1010 2349 256 887
Fragments arranged by size:
2349 1010 887 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4502
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4502
AvaI C'yCGr_G
Cuts at: 241 737 1522 241
Size: 496 785 3221
Fragments arranged by size:
3221 785 496
BamHI G'GATC_C
Cuts at: 1527 1527
Size: 4502
BanI G'GyrC_C
Cuts at: 1518 1932 2180 2215 1518
Size: 414 248 35 3805
Fragments arranged by size:
3805 414 248 35
BanII G_rGCy'C
Cuts at: 359 1516 2546 359
Size: 1157 1030 2315
Fragments arranged by size:
2315 1157 1030
BbeI G_GCGC'C
Cuts at: 1936 2184 1936
Size: 248 4254
BbsI GAAGACnn'nnnn_
Cuts at: 477 477
Size: 4502
BciVI GTATCCnnnnn_n'
Cuts at: 2400 3456 4502 2400
Size: 1056 1046 2400
Fragments arranged by size:
2400 1056 1046
BclI T'GATC_A
Cuts at: 2022 2022
Size: 4502
BglII A'GATC_T
Cuts at: 2017 4054 2017
Size: 2037 2465
BlpI GC'TnA_GC
Cuts at: 1614 1614
Size: 4502
Bme1580I G_kGCm'C
Cuts at: 820 2127 2220 3344 820
Size: 1307 93 1124 1978
Fragments arranged by size:
1978 1307 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 216 1714 1795 2130 4274 216
Size: 1498 81 335 2144 444
Fragments arranged by size:
2144 1498 444 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2869 2869
Size: 4502
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 221 280 1289 221
Size: 59 1009 3434
Fragments arranged by size:
3434 1009 59
Bpu10I CC'TnA_GC
Cuts at: 345 345
Size: 4502
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 998 1678 2557 3271 3569 998
Size: 680 879 714 298 1931
Fragments arranged by size:
1931 879 714 680 298
BsaI GGTCTCn'nnnn_
Cuts at: 4206 4206
Size: 4502
BsaAI yAC'GTr
Cuts at: 1001 1855 1001
Size: 854 3648
BsaBI GATnn'nnATC
Cuts at: 6 2016 4053 6
Size: 2010 2037 455
Fragments arranged by size:
2037 2010 455
BsaHI Gr'CG_yC
Cuts at: 1933 2181 2883 4379 1933
Size: 248 702 1496 2056
Fragments arranged by size:
2056 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1691 2212 2890 3301 3448 3722 1691
Size: 521 678 411 147 274 2471
Fragments arranged by size:
2471 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 893 923 893
Size: 30 4472
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 136 166 136
Size: 30 4472
BseYI C'CCAG_C
Cuts at: 1200 1927 2685 3350 3758 1200
Size: 727 758 665 408 1944
Fragments arranged by size:
1944 758 727 665 408
BsiEI CG_ry'CG
Cuts at: 104 2044 2090 3320 104
Size: 1940 46 1230 1286
Fragments arranged by size:
1940 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 286 359 820 1516 2294 2484 3344 286
Size: 73 461 696 778 190 860 1444
Fragments arranged by size:
1444 860 778 696 461 190 73
BsmI GAATG_Cn'
Cuts at: 778 778
Size: 4502
Bsp1286I G_dGCh'C
Cuts at: 286 359 820 1516 2127 2220 2294 2484 2546
Size: 73 461 696 611 93 74 190 62
Cuts at: 2546 3344 286
Size: 798 1444
Fragments arranged by size:
1444 798 696 611 461 190 93 74
73 62
BspHI T'CATG_A
Cuts at: 2934 2934
Size: 4502
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1552 1766 2068 2449 1552
Size: 214 302 381 3605
Fragments arranged by size:
3605 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2794 2848 4332 4497 50
Size: 2744 54 1484 165 55
Fragments arranged by size:
2744 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 531 821 1064 2414 531
Size: 290 243 1350 2619
Fragments arranged by size:
2619 1350 290 243
BsrFI r'CCGG_y
Cuts at: 2500 2681 2500
Size: 181 4321
BssSI C'ACGA_G
Cuts at: 2773 3481 2773
Size: 708 3794
BstAPI GCAn_nnn'nTGC
Cuts at: 1066 1879 1066
Size: 813 3689
BstBI TT'CG_AA
Cuts at: 275 2863 275
Size: 2588 1914
BstEII G'GTnAC_C
Cuts at: 1359 1359
Size: 4502
BstYI r'GATC_y
Cuts at: 1 678 1527 1994 2017 2352 2598 2904 2916
Size: 677 849 467 23 335 246 306 12
Cuts at: 2916 3002 4054 1
Size: 86 1052 449
Fragments arranged by size:
1052 849 677 467 449 335 306 246
86 23 12
BstZ17I GTA'TAC
Cuts at: 1163 1163
Size: 4502
Bsu36I CC'TnA_GG
Cuts at: 476 884 476
Size: 408 4094
BtgI C'CryG_G
Cuts at: 107 107
Size: 4502
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 600 2452 2633 2796 600
Size: 1852 181 163 2306
Fragments arranged by size:
2306 1852 181 163
BtsI GCAGTG_nn'
Cuts at: 883 1745 883
Size: 862 3640
DraI TTT'AAA
Cuts at: 726 1095 1501 726
Size: 369 406 3727
Fragments arranged by size:
3727 406 369
DrdI GACnn_nn'nnGTC
Cuts at: 2208 3552 2208
Size: 1344 3158
EaeI y'GGCC_r
Cuts at: 2087 2261 2652 2679 4024 2087
Size: 174 391 27 1345 2565
Fragments arranged by size:
2565 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2087 2087
Size: 4502
EarI CTCTTCn'nnn_
Cuts at: 656 2525 2735 656
Size: 1869 210 2423
Fragments arranged by size:
2423 1869 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 127 2710 3452 3598 4312 127
Size: 2583 742 146 714 317
Fragments arranged by size:
2583 742 714 317 146
EcoICRI GAG'CTC
Cuts at: 357 1514 357
Size: 1157 3345
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 221 280 503 1289 2327 2759 3112 3774 221
Size: 59 223 786 1038 432 353 662 949
Fragments arranged by size:
1038 949 786 662 432 353 223 59
EcoO109I rG'GnC_Cy
Cuts at: 1641 1641
Size: 4502
EcoRI G'AATT_C
Cuts at: 1506 1506
Size: 4502
EcoRV GAT'ATC
Cuts at: 647 647
Size: 4502
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2521 2521
Size: 4502
FspI TGC'GCA
Cuts at: 2283 2283
Size: 4502
HaeII r_GCGC'y
Cuts at: 1936 2184 3414 4185 1936
Size: 248 1230 771 2253
Fragments arranged by size:
2253 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 341 373 670 702 958 990 2344 2374 2376
Size: 32 297 32 256 32 1354 30 2
Cuts at: 2376 2406 341
Size: 30 2437
Fragments arranged by size:
2437 1354 297 256 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1541 1541
Size: 4502
KasI G'GCGC_C
Cuts at: 1932 2180 1932
Size: 248 4254
KpnI G_GTAC'C
Cuts at: 1522 1522
Size: 4502
MluI A'CGCG_T
Cuts at: 3935 3935
Size: 4502
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3261 3445 3261
Size: 184 4318
MscI TGG'CCA
Cuts at: 2263 2263
Size: 4502
MslI CAynn'nnrTG
Cuts at: 2491 2618 2491
Size: 127 4375
MspA1I CmG'CkG
Cuts at: 1072 1613 1927 2287 3071 3316 1072
Size: 541 314 360 784 245 2258
Fragments arranged by size:
2258 784 541 360 314 245
NaeI GCC'GGC
Cuts at: 2683 2683
Size: 4502
NarI GG'CG_CC
Cuts at: 1933 2181 1933
Size: 248 4254
NcoI C'CATG_G
Cuts at: 107 107
Size: 4502
NgoMIV G'CCGG_C
Cuts at: 2681 2681
Size: 4502
NspI r_CATG'y
Cuts at: 317 642 1555 2055 3658 317
Size: 325 913 500 1603 1161
Fragments arranged by size:
1603 1161 913 500 325
PciI A'CATG_T
Cuts at: 313 3654 313
Size: 3341 1161
PflMI CCAn_nnn'nTGG
Cuts at: 626 919 626
Size: 293 4209
PmeI GTTT'AAAC
Cuts at: 1501 1501
Size: 4502
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 893 925 2948 2980 893
Size: 32 2023 32 2415
Fragments arranged by size:
2415 2023 32 32
PspXI vC'TCGA_Gb
Cuts at: 241 241
Size: 4502
PstI C_TGCA'G
Cuts at: 495 1044 1549 2234 495
Size: 549 505 685 2763
Fragments arranged by size:
2763 685 549 505
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4502
PvuII CAG'CTG
Cuts at: 1072 1927 2287 1072
Size: 855 360 3287
Fragments arranged by size:
3287 855 360
RsrII CG'GwC_CG
Cuts at: 2697 2697
Size: 4502
SacI G_AGCT'C
Cuts at: 359 1516 359
Size: 1157 3345
SalI G'TCGA_C
Cuts at: 1539 1539
Size: 4502
SapI GCTCTTCn'nnn_
Cuts at: 2525 2735 2525
Size: 210 4292
SbfI CC_TGCA'GG
Cuts at: 1549 1549
Size: 4502
ScaI AGT'ACT
Cuts at: 4019 4019
Size: 4502
SfcI C'TryA_G
Cuts at: 33 416 491 1040 1252 1545 2230 3198 3389
Size: 383 75 549 212 293 685 968 191
Cuts at: 3389 33
Size: 1146
Fragments arranged by size:
1146 968 685 549 383 293 212 191
75
SfoI GGC'GCC
Cuts at: 1934 2182 1934
Size: 248 4254
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4502
SmaI CCC'GGG
Cuts at: 739 1524 739
Size: 785 3717
SmlI C'TyrA_G
Cuts at: 241 1013 1657 2572 3286 3548 241
Size: 772 644 915 714 262 1195
Fragments arranged by size:
1195 915 772 714 644 262
SnaBI TAC'GTA
Cuts at: 1001 1001
Size: 4502
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4502
SphI G_CATG'C
Cuts at: 642 1555 2055 642
Size: 913 500 3089
Fragments arranged by size:
3089 913 500
SspI AAT'ATT
Cuts at: 987 1227 987
Size: 240 4262
StuI AGG'CCT
Cuts at: 456 456
Size: 4502
StyI C'CwwG_G
Cuts at: 107 1636 1990 107
Size: 1529 354 2619
Fragments arranged by size:
2619 1529 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 176 2085 2218 2677 2886 176
Size: 1909 133 459 209 1792
Fragments arranged by size:
1909 1792 459 209 133
TatI w'GTAC_w
Cuts at: 1470 4017 1470
Size: 2547 1955
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4019 4051 4019
Size: 32 4470
Tth111I GACn'n_nGTC
Cuts at: 2299 2299
Size: 4502
XbaI T'CTAG_A
Cuts at: 1533 1533
Size: 4502
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1997 1997
Size: 4502
XhoI C'TCGA_G
Cuts at: 241 241
Size: 4502
XmaI C'CCGG_G
Cuts at: 737 1522 737
Size: 785 3717
XmnI GAAnn'nnTTC
Cuts at: 908 908
Size: 4502
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AloI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PflMI PmeI PpiI PspXI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmgBI BmtI BsgI BsiWI BsmBI BspEI BsrGI BssHII
BstXI ClaI CspCI DraIII EcoNI FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PfoI PmlI PpuMI PshAI PsiI PspOMI PsrI
SacII SanDI SexAI SfiI SgrAI SrfI SwaI ZraI