Restriction map for
pF1KB8670
(Circular) (Six-base) MAPSORT of: pF1KB8670.seq Check: 9205 from: 1 to: 4505
pF1KB8670 4505 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AccI GT'mk_AC
Cuts at: 1543 1543
Size: 4505
Acc65I G'GTAC_C
Cuts at: 1521 1521
Size: 4505
AclI AA'CG_TT
Cuts at: 959 4345 959
Size: 3386 1119
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 288 678 2330 2762 3115 3777 288
Size: 390 1652 432 353 662 1016
Fragments arranged by size:
1652 1016 662 432 390 353
AflIII A'CryG_T
Cuts at: 1173 1857 3657 3938 1173
Size: 684 1800 281 1740
Fragments arranged by size:
1800 1740 684 281
AleI CACnn'nnGTG
Cuts at: 1075 1075
Size: 4505
AlwNI CAG_nnn'CTG
Cuts at: 1810 3248 1810
Size: 1438 3067
ApaI G_GGCC'C
Cuts at: 359 365 359
Size: 6 4499
ApaLI G'TGCA_C
Cuts at: 3343 3343
Size: 4505
ApoI r'AATT_y
Cuts at: 749 1196 1509 3858 4114 749
Size: 447 313 2349 256 1140
Fragments arranged by size:
2349 1140 447 313 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4505
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4505
AvaI C'yCGr_G
Cuts at: 312 358 1525 312
Size: 46 1167 3292
Fragments arranged by size:
3292 1167 46
BamHI G'GATC_C
Cuts at: 392 1530 392
Size: 1138 3367
BanI G'GyrC_C
Cuts at: 240 342 373 1379 1521 1935 2183 2218 240
Size: 102 31 1006 142 414 248 35 2527
Fragments arranged by size:
2527 1006 414 248 142 102 35 31
BanII G_rGCy'C
Cuts at: 138 359 365 590 660 1519 2549 138
Size: 221 6 225 70 859 1030 2094
Fragments arranged by size:
2094 1030 859 225 221 70 6
BbeI G_GCGC'C
Cuts at: 377 1939 2187 377
Size: 1562 248 2695
Fragments arranged by size:
2695 1562 248
BbsI GAAGACnn'nnnn_
Cuts at: 282 684 282
Size: 402 4103
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 253 287 253
Size: 34 4471
BciVI GTATCCnnnnn_n'
Cuts at: 2403 3459 4505 2403
Size: 1056 1046 2403
Fragments arranged by size:
2403 1056 1046
BclI T'GATC_A
Cuts at: 2025 2025
Size: 4505
BglII A'GATC_T
Cuts at: 2020 4057 2020
Size: 2037 2468
BlpI GC'TnA_GC
Cuts at: 154 1617 154
Size: 1463 3042
Bme1580I G_kGCm'C
Cuts at: 182 245 347 359 365 407 1088 2130 2223
Size: 63 102 12 6 42 681 1042 93
Cuts at: 2223 3347 182
Size: 1124 1340
Fragments arranged by size:
1340 1124 1042 681 102 93 63 42
12 6
BmgBI CAC'GTC
Cuts at: 1452 1452
Size: 4505
BmrI ACTGGGnnnn_n'
Cuts at: 1717 1798 2133 4277 1717
Size: 81 335 2144 1945
Fragments arranged by size:
2144 1945 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2872 2872
Size: 4505
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 436 1420 436
Size: 984 3521
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1681 2560 3274 3572 1681
Size: 879 714 298 2614
Fragments arranged by size:
2614 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 648 935 4209 648
Size: 287 3274 944
Fragments arranged by size:
3274 944 287
BsaAI yAC'GTr
Cuts at: 258 1858 258
Size: 1600 2905
BsaBI GATnn'nnATC
Cuts at: 6 2019 4056 6
Size: 2013 2037 455
Fragments arranged by size:
2037 2013 455
BsaHI Gr'CG_yC
Cuts at: 374 1936 2184 2886 4382 374
Size: 1562 248 702 1496 497
Fragments arranged by size:
1562 1496 702 497 248
BsaWI w'CCGG_w
Cuts at: 1694 2215 2893 3304 3451 3725 1694
Size: 521 678 411 147 274 2474
Fragments arranged by size:
2474 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 944 974 1393 1423 944
Size: 30 419 30 4026
Fragments arranged by size:
4026 419 30 30
BseYI C'CCAG_C
Cuts at: 130 141 168 321 346 933 1930 2688 3353
Size: 11 27 153 25 587 997 758 665
Cuts at: 3353 3761 130
Size: 408 874
Fragments arranged by size:
997 874 758 665 587 408 153 27
25 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1172 1313 1172
Size: 141 4364
BsiEI CG_ry'CG
Cuts at: 104 285 382 654 1395 2047 2093 3323 104
Size: 181 97 272 741 652 46 1230 1286
Fragments arranged by size:
1286 1230 741 652 272 181 97 46
BsiHKAI G_wGCw'C
Cuts at: 660 1519 2297 2487 3347 660
Size: 859 778 190 860 1818
Fragments arranged by size:
1818 860 859 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 603 603
Size: 4505
Bsp1286I G_dGCh'C
Cuts at: 138 182 245 347 359 365 407 590 660
Size: 44 63 102 12 6 42 183 70
Cuts at: 660 1088 1519 2130 2223 2297 2487 2549 3347
Size: 428 431 611 93 74 190 62 798
Cuts at: 3347 138
Size: 1296
Fragments arranged by size:
1296 798 611 431 428 190 183 102
93 74 70 63 62 44 42 12
6
BspHI T'CATG_A
Cuts at: 2937 2937
Size: 4505
BspMI ACCTGCnnnn'nnnn_
Cuts at: 630 1052 1555 1769 2071 2452 630
Size: 422 503 214 302 381 2683
Fragments arranged by size:
2683 503 422 381 302 214
BsrBI CCG'CTC
Cuts at: 50 158 2797 2851 4335 4500 50
Size: 108 2639 54 1484 165 55
Fragments arranged by size:
2639 1484 165 108 55 54
BsrDI GCAATG_nn'
Cuts at: 727 2417 727
Size: 1690 2815
BsrFI r'CCGG_y
Cuts at: 209 370 2503 2684 209
Size: 161 2133 181 2030
Fragments arranged by size:
2133 2030 181 161
BssHII G'CGCG_C
Cuts at: 267 267
Size: 4505
BssSI C'ACGA_G
Cuts at: 2776 3484 2776
Size: 708 3797
BstAPI GCAn_nnn'nTGC
Cuts at: 1882 1882
Size: 4505
BstBI TT'CG_AA
Cuts at: 2866 2866
Size: 4505
BstEII G'GTnAC_C
Cuts at: 1476 1476
Size: 4505
BstXI CCAn_nnnn'nTGG
Cuts at: 114 933 114
Size: 819 3686
BstYI r'GATC_y
Cuts at: 1 392 1530 1997 2020 2355 2601 2907 2919
Size: 391 1138 467 23 335 246 306 12
Cuts at: 2919 3005 4057 1
Size: 86 1052 449
Fragments arranged by size:
1138 1052 467 449 391 335 306 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 620 620
Size: 4505
BtgI C'CryG_G
Cuts at: 107 245 774 1417 107
Size: 138 529 643 3195
Fragments arranged by size:
3195 643 529 138
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2455 2636 2799 2455
Size: 181 163 4161
Fragments arranged by size:
4161 181 163
BtsI GCAGTG_nn'
Cuts at: 463 1160 1748 463
Size: 697 588 3220
Fragments arranged by size:
3220 697 588
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 918 953 1202 1237 1242 1277 918
Size: 35 249 35 5 35 4146
Fragments arranged by size:
4146 249 35 35 35 5
DraI TTT'AAA
Cuts at: 1504 1504
Size: 4505
DraIII CAC_nnn'GTG
Cuts at: 1476 1476
Size: 4505
DrdI GACnn_nn'nnGTC
Cuts at: 2211 3555 2211
Size: 1344 3161
EaeI y'GGCC_r
Cuts at: 191 651 795 995 1484 2090 2264 2655 2682
Size: 460 144 200 489 606 174 391 27
Cuts at: 2682 4027 191
Size: 1345 669
Fragments arranged by size:
1345 669 606 489 460 391 200 174
144 27
EagI C'GGCC_G
Cuts at: 651 2090 651
Size: 1439 3066
EarI CTCTTCn'nnn_
Cuts at: 2528 2738 2528
Size: 210 4295
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 250 993 2713 3455 3601 4315 250
Size: 743 1720 742 146 714 440
Fragments arranged by size:
1720 743 742 714 440 146
EcoICRI GAG'CTC
Cuts at: 658 1517 658
Size: 859 3646
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 288 436 678 1420 2330 2762 3115 3777 288
Size: 148 242 742 910 432 353 662 1016
Fragments arranged by size:
1016 910 742 662 432 353 242 148
EcoO109I rG'GnC_Cy
Cuts at: 362 1644 362
Size: 1282 3223
EcoRI G'AATT_C
Cuts at: 1196 1509 1196
Size: 313 4192
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2524 2524
Size: 4505
FspI TGC'GCA
Cuts at: 2286 2286
Size: 4505
HaeII r_GCGC'y
Cuts at: 115 201 322 377 1939 2187 3417 4188 115
Size: 86 121 55 1562 248 1230 771 432
Fragments arranged by size:
1562 1230 771 432 248 121 86 55
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1298 1330 2347 2377 2379 2409 1298
Size: 32 1017 30 2 30 3394
Fragments arranged by size:
3394 1017 32 30 30 2
HincII GTy'rAC
Cuts at: 674 1544 674
Size: 870 3635
KasI G'GCGC_C
Cuts at: 373 1935 2183 373
Size: 1562 248 2695
Fragments arranged by size:
2695 1562 248
KpnI G_GTAC'C
Cuts at: 1525 1525
Size: 4505
MluI A'CGCG_T
Cuts at: 3938 3938
Size: 4505
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 563 3264 3448 563
Size: 2701 184 1620
Fragments arranged by size:
2701 1620 184
MscI TGG'CCA
Cuts at: 797 1486 2266 797
Size: 689 780 3036
Fragments arranged by size:
3036 780 689
MslI CAynn'nnrTG
Cuts at: 1075 2494 2621 1075
Size: 1419 127 2959
Fragments arranged by size:
2959 1419 127
MspA1I CmG'CkG
Cuts at: 1050 1091 1616 1930 2290 3074 3319 1050
Size: 41 525 314 360 784 245 2236
Fragments arranged by size:
2236 784 525 360 314 245 41
NaeI GCC'GGC
Cuts at: 211 372 2686 211
Size: 161 2314 2030
Fragments arranged by size:
2314 2030 161
NarI GG'CG_CC
Cuts at: 374 1936 2184 374
Size: 1562 248 2695
Fragments arranged by size:
2695 1562 248
NcoI C'CATG_G
Cuts at: 107 1417 107
Size: 1310 3195
NgoMIV G'CCGG_C
Cuts at: 209 370 2684 209
Size: 161 2314 2030
Fragments arranged by size:
2314 2030 161
NspI r_CATG'y
Cuts at: 1136 1177 1460 1558 2058 3661 1136
Size: 41 283 98 500 1603 1980
Fragments arranged by size:
1980 1603 500 283 98 41
PciI A'CATG_T
Cuts at: 1173 3657 1173
Size: 2484 2021
PflMI CCAn_nnn'nTGG
Cuts at: 258 258
Size: 4505
PfoI T'CCnGG_A
Cuts at: 202 202
Size: 4505
PmeI GTTT'AAAC
Cuts at: 1504 1504
Size: 4505
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2951 2983 2951
Size: 32 4473
PspOMI G'GGCC_C
Cuts at: 355 361 355
Size: 6 4499
PstI C_TGCA'G
Cuts at: 641 1049 1552 2237 641
Size: 408 503 685 2909
Fragments arranged by size:
2909 685 503 408
PvuI CG_AT'CG
Cuts at: 104 285 104
Size: 181 4324
PvuII CAG'CTG
Cuts at: 1050 1930 2290 1050
Size: 880 360 3265
Fragments arranged by size:
3265 880 360
RsrII CG'GwC_CG
Cuts at: 2700 2700
Size: 4505
SacI G_AGCT'C
Cuts at: 660 1519 660
Size: 859 3646
SalI G'TCGA_C
Cuts at: 1542 1542
Size: 4505
SapI GCTCTTCn'nnn_
Cuts at: 2528 2738 2528
Size: 210 4295
SbfI CC_TGCA'GG
Cuts at: 1552 1552
Size: 4505
ScaI AGT'ACT
Cuts at: 4022 4022
Size: 4505
SfcI C'TryA_G
Cuts at: 33 637 1045 1548 2233 3201 3392 33
Size: 604 408 503 685 968 191 1146
Fragments arranged by size:
1146 968 685 604 503 408 191
SfoI GGC'GCC
Cuts at: 375 1937 2185 375
Size: 1562 248 2695
Fragments arranged by size:
2695 1562 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4505
SgrAI Cr'CCGG_yG
Cuts at: 370 370
Size: 4505
SmaI CCC'GGG
Cuts at: 360 1527 360
Size: 1167 3338
SmlI C'TyrA_G
Cuts at: 1660 2575 3289 3551 1660
Size: 915 714 262 2614
Fragments arranged by size:
2614 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4505
SphI G_CATG'C
Cuts at: 1136 1558 2058 1136
Size: 422 500 3583
Fragments arranged by size:
3583 500 422
SrfI GCCC'GGGC
Cuts at: 360 360
Size: 4505
StyI C'CwwG_G
Cuts at: 107 1417 1639 1993 107
Size: 1310 222 354 2619
Fragments arranged by size:
2619 1310 354 222
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 925 1266 1381 2088 2221 2680 2889 925
Size: 341 115 707 133 459 209 2541
Fragments arranged by size:
2541 707 459 341 209 133 115
TatI w'GTAC_w
Cuts at: 4020 4020
Size: 4505
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 719 751 4022 4054 719
Size: 32 3271 32 1170
Fragments arranged by size:
3271 1170 32 32
Tth111I GACn'n_nGTC
Cuts at: 2302 2302
Size: 4505
XbaI T'CTAG_A
Cuts at: 1536 1536
Size: 4505
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 814 2000 114
Size: 700 1186 2619
Fragments arranged by size:
2619 1186 700
XmaI C'CCGG_G
Cuts at: 358 1525 358
Size: 1167 3338
XmnI GAAnn'nnTTC
Cuts at: 708 1244 708
Size: 536 3969
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AleI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI
PfoI PmeI PpiI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SpeI SphI SrfI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AlfI AloI
AscI AvrII BaeI BbvCI BfrBI BglI BmtI Bpu10I
BseRI BsiWI BsmI BspEI BsrGI BstZ17I ClaI EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PmlI PpuMI PshAI
PsiI PspXI PsrI SacII SanDI SexAI SfiI SnaBI
SspI StuI SwaI XhoI ZraI