Restriction map for
pF1KB8672
(Circular) (Six-base) MAPSORT of: pF1KB8672.seq Check: 5622 from: 1 to: 4826
pF1KB8672 4826 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AccI GT'mk_AC
Cuts at: 296 1864 296
Size: 1568 3258
Acc65I G'GTAC_C
Cuts at: 1842 1842
Size: 4826
AclI AA'CG_TT
Cuts at: 4666 4666
Size: 4826
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1679 2651 3083 3436 4098 1679
Size: 972 432 353 662 2407
Fragments arranged by size:
2407 972 662 432 353
AflIII A'CryG_T
Cuts at: 1400 2178 3978 4259 1400
Size: 778 1800 281 1967
Fragments arranged by size:
1967 1800 778 281
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 733 765 733
Size: 32 4794
AlwNI CAG_nnn'CTG
Cuts at: 319 1116 1545 2131 3569 319
Size: 797 429 586 1438 1576
Fragments arranged by size:
1576 1438 797 586 429
ApaLI G'TGCA_C
Cuts at: 3664 3664
Size: 4826
ApoI r'AATT_y
Cuts at: 181 537 855 1830 4179 4435 181
Size: 356 318 975 2349 256 572
Fragments arranged by size:
2349 975 572 356 318 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4826
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4826
AvaI C'yCGr_G
Cuts at: 1846 1846
Size: 4826
BamHI G'GATC_C
Cuts at: 151 1851 151
Size: 1700 3126
BanI G'GyrC_C
Cuts at: 1842 2256 2504 2539 1842
Size: 414 248 35 4129
Fragments arranged by size:
4129 414 248 35
BanII G_rGCy'C
Cuts at: 266 980 1840 2870 266
Size: 714 860 1030 2222
Fragments arranged by size:
2222 1030 860 714
BbeI G_GCGC'C
Cuts at: 2260 2508 2260
Size: 248 4578
BbsI GAAGACnn'nnnn_
Cuts at: 608 608
Size: 4826
BciVI GTATCCnnnnn_n'
Cuts at: 759 2724 3780 4826 759
Size: 1965 1056 1046 759
Fragments arranged by size:
1965 1056 1046 759
BclI T'GATC_A
Cuts at: 512 2346 512
Size: 1834 2992
BglI GCCn_nnn'nGGC
Cuts at: 676 676
Size: 4826
BglII A'GATC_T
Cuts at: 2341 4378 2341
Size: 2037 2789
BlpI GC'TnA_GC
Cuts at: 974 1938 974
Size: 964 3862
Bme1580I G_kGCm'C
Cuts at: 1460 2451 2544 3668 1460
Size: 991 93 1124 2618
Fragments arranged by size:
2618 1124 991 93
BmrI ACTGGGnnnn_n'
Cuts at: 360 703 2038 2119 2454 4598 360
Size: 343 1335 81 335 2144 588
Fragments arranged by size:
2144 1335 588 343 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3193 3193
Size: 4826
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 907 907
Size: 4826
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2002 2881 3595 3893 2002
Size: 879 714 298 2935
Fragments arranged by size:
2935 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1011 4530 1011
Size: 3519 1307
BsaAI yAC'GTr
Cuts at: 2179 2179
Size: 4826
BsaBI GATnn'nnATC
Cuts at: 6 2340 4377 6
Size: 2334 2037 455
Fragments arranged by size:
2334 2037 455
BsaHI Gr'CG_yC
Cuts at: 1490 2257 2505 3207 4703 1490
Size: 767 248 702 1496 1613
Fragments arranged by size:
1613 1496 767 702 248
BsaWI w'CCGG_w
Cuts at: 2015 2536 3214 3625 3772 4046 2015
Size: 521 678 411 147 274 2795
Fragments arranged by size:
2795 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 798 2251 3009 3674 4082 798
Size: 1453 758 665 408 1542
Fragments arranged by size:
1542 1453 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 2368 2414 3644 104
Size: 2264 46 1230 1286
Fragments arranged by size:
2264 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 266 1840 2618 2808 3668 266
Size: 1574 778 190 860 1424
Fragments arranged by size:
1574 1424 860 778 190
BsmI GAATG_Cn'
Cuts at: 369 633 369
Size: 264 4562
Bsp1286I G_dGCh'C
Cuts at: 266 980 1460 1840 2451 2544 2618 2808 2870
Size: 714 480 380 611 93 74 190 62
Cuts at: 2870 3668 266
Size: 798 1424
Fragments arranged by size:
1424 798 714 611 480 380 190 93
74 62
BspHI T'CATG_A
Cuts at: 3258 3258
Size: 4826
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1876 2090 2392 2773 1876
Size: 214 302 381 3929
Fragments arranged by size:
3929 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3118 3172 4656 4821 50
Size: 3068 54 1484 165 55
Fragments arranged by size:
3068 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1493 1709 2738 1493
Size: 216 1029 3581
Fragments arranged by size:
3581 1029 216
BsrFI r'CCGG_y
Cuts at: 2824 3005 2824
Size: 181 4645
BsrGI T'GTAC_A
Cuts at: 1590 1590
Size: 4826
BssSI C'ACGA_G
Cuts at: 3097 3805 3097
Size: 708 4118
BstAPI GCAn_nnn'nTGC
Cuts at: 532 2203 532
Size: 1671 3155
BstBI TT'CG_AA
Cuts at: 3187 3187
Size: 4826
BstXI CCAn_nnnn'nTGG
Cuts at: 759 759
Size: 4826
BstYI r'GATC_y
Cuts at: 1 151 1851 2318 2341 2676 2922 3228 3240
Size: 150 1700 467 23 335 246 306 12
Cuts at: 3240 3326 4378 1
Size: 86 1052 449
Fragments arranged by size:
1700 1052 467 449 335 306 246 150
86 23 12
BtgI C'CryG_G
Cuts at: 480 674 480
Size: 194 4632
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2776 2957 3120 2776
Size: 181 163 4482
Fragments arranged by size:
4482 181 163
BtsI GCAGTG_nn'
Cuts at: 2069 2069
Size: 4826
DraI TTT'AAA
Cuts at: 1825 1825
Size: 4826
DrdI GACnn_nn'nnGTC
Cuts at: 2532 3876 2532
Size: 1344 3482
EaeI y'GGCC_r
Cuts at: 158 542 684 2411 2585 2976 3003 4348 158
Size: 384 142 1727 174 391 27 1345 636
Fragments arranged by size:
1727 1345 636 391 384 174 142 27
EagI C'GGCC_G
Cuts at: 2411 2411
Size: 4826
EarI CTCTTCn'nnn_
Cuts at: 824 2849 3059 824
Size: 2025 210 2591
Fragments arranged by size:
2591 2025 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3034 3776 3922 4636 3034
Size: 742 146 714 3224
Fragments arranged by size:
3224 742 714 146
EcoICRI GAG'CTC
Cuts at: 264 1838 264
Size: 1574 3252
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 907 1679 2651 3083 3436 4098 907
Size: 772 972 432 353 662 1635
Fragments arranged by size:
1635 972 772 662 432 353
EcoO109I rG'GnC_Cy
Cuts at: 1965 1965
Size: 4826
EcoRI G'AATT_C
Cuts at: 1830 1830
Size: 4826
EcoRV GAT'ATC
Cuts at: 1329 1329
Size: 4826
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 838 2845 838
Size: 2007 2819
FspI TGC'GCA
Cuts at: 2607 2607
Size: 4826
HaeII r_GCGC'y
Cuts at: 2260 2508 3738 4509 2260
Size: 248 1230 771 2577
Fragments arranged by size:
2577 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 326 358 476 508 1318 1350 2668 2698 2700
Size: 32 118 32 810 32 1318 30 2
Cuts at: 2700 2730 326
Size: 30 2422
Fragments arranged by size:
2422 1318 810 118 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 656 1865 656
Size: 1209 3617
HindIII A'AGCT_T
Cuts at: 1661 1661
Size: 4826
HpaI GTT'AAC
Cuts at: 656 656
Size: 4826
KasI G'GCGC_C
Cuts at: 2256 2504 2256
Size: 248 4578
KpnI G_GTAC'C
Cuts at: 1846 1846
Size: 4826
MluI A'CGCG_T
Cuts at: 4259 4259
Size: 4826
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3585 3769 3585
Size: 184 4642
MscI TGG'CCA
Cuts at: 544 686 2587 544
Size: 142 1901 2783
Fragments arranged by size:
2783 1901 142
MslI CAynn'nnrTG
Cuts at: 436 458 757 2815 2942 436
Size: 22 299 2058 127 2320
Fragments arranged by size:
2320 2058 299 127 22
MspA1I CmG'CkG
Cuts at: 852 1937 2251 2611 3395 3640 852
Size: 1085 314 360 784 245 2038
Fragments arranged by size:
2038 1085 784 360 314 245
NaeI GCC'GGC
Cuts at: 3007 3007
Size: 4826
NarI GG'CG_CC
Cuts at: 2257 2505 2257
Size: 248 4578
NcoI C'CATG_G
Cuts at: 480 674 480
Size: 194 4632
NgoMIV G'CCGG_C
Cuts at: 3005 3005
Size: 4826
NspI r_CATG'y
Cuts at: 1404 1879 2379 3982 1404
Size: 475 500 1603 2248
Fragments arranged by size:
2248 1603 500 475
PciI A'CATG_T
Cuts at: 1400 3978 1400
Size: 2578 2248
PfoI T'CCnGG_A
Cuts at: 1433 1433
Size: 4826
PmeI GTTT'AAAC
Cuts at: 1825 1825
Size: 4826
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3272 3304 3272
Size: 32 4794
PshAI GACnn'nnGTC
Cuts at: 292 292
Size: 4826
PsiI TTA'TAA
Cuts at: 1582 1582
Size: 4826
PstI C_TGCA'G
Cuts at: 1873 2558 1873
Size: 685 4141
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4826
PvuII CAG'CTG
Cuts at: 852 2251 2611 852
Size: 1399 360 3067
Fragments arranged by size:
3067 1399 360
RsrII CG'GwC_CG
Cuts at: 3021 3021
Size: 4826
SacI G_AGCT'C
Cuts at: 266 1840 266
Size: 1574 3252
SalI G'TCGA_C
Cuts at: 1863 1863
Size: 4826
SapI GCTCTTCn'nnn_
Cuts at: 2849 3059 2849
Size: 210 4616
SbfI CC_TGCA'GG
Cuts at: 1873 1873
Size: 4826
ScaI AGT'ACT
Cuts at: 549 4343 549
Size: 3794 1032
SfcI C'TryA_G
Cuts at: 33 1178 1869 2554 3522 3713 33
Size: 1145 691 685 968 191 1146
Fragments arranged by size:
1146 1145 968 691 685 191
SfoI GGC'GCC
Cuts at: 2258 2506 2258
Size: 248 4578
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4826
SmaI CCC'GGG
Cuts at: 1848 1848
Size: 4826
SmlI C'TyrA_G
Cuts at: 1981 2896 3610 3872 1981
Size: 915 714 262 2935
Fragments arranged by size:
2935 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4826
SphI G_CATG'C
Cuts at: 1879 2379 1879
Size: 500 4326
SspI AAT'ATT
Cuts at: 531 1428 531
Size: 897 3929
StyI C'CwwG_G
Cuts at: 155 195 480 674 1960 2314 155
Size: 40 285 194 1286 354 2667
Fragments arranged by size:
2667 1286 354 285 194 40
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 342 895 1498 2409 2542 3001 3210 342
Size: 553 603 911 133 459 209 1958
Fragments arranged by size:
1958 911 603 553 459 209 133
TatI w'GTAC_w
Cuts at: 547 1590 4341 547
Size: 1043 2751 1032
Fragments arranged by size:
2751 1043 1032
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4343 4375 4343
Size: 32 4794
Tth111I GACn'n_nGTC
Cuts at: 2623 2623
Size: 4826
XbaI T'CTAG_A
Cuts at: 1857 1857
Size: 4826
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2321 2321
Size: 4826
XmaI C'CCGG_G
Cuts at: 1846 1846
Size: 4826
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AloI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PfoI PmeI PpiI PshAI PsiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI
BmtI Bpu10I BsaXI BseRI BsgI BsiWI BsmBI BspEI
BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
FseI FspAI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PmlI PpuMI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI StuI
SwaI XhoI XmnI ZraI