Restriction map for
pF1KB8673
(Circular) (Six-base) MAPSORT of: pF1KB8673.seq Check: 9009 from: 1 to: 5084
pF1KB8673 5084 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AccI GT'mk_AC
Cuts at: 2122 2122
Size: 5084
Acc65I G'GTAC_C
Cuts at: 2100 2100
Size: 5084
AclI AA'CG_TT
Cuts at: 4924 4924
Size: 5084
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 512 1275 1424 2909 3341 3694 4356 512
Size: 763 149 1485 432 353 662 1240
Fragments arranged by size:
1485 1240 763 662 432 353 149
AflIII A'CryG_T
Cuts at: 1061 2436 4236 4517 1061
Size: 1375 1800 281 1628
Fragments arranged by size:
1800 1628 1375 281
AlwNI CAG_nnn'CTG
Cuts at: 2389 3827 2389
Size: 1438 3646
ApaLI G'TGCA_C
Cuts at: 3922 3922
Size: 5084
ApoI r'AATT_y
Cuts at: 830 1033 1078 1150 1580 2088 4437 4693 830
Size: 203 45 72 430 508 2349 256 1221
Fragments arranged by size:
2349 1221 508 430 256 203 72 45
AseI AT'TA_AT
Cuts at: 20 20
Size: 5084
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5084
AvaI C'yCGr_G
Cuts at: 1189 1226 1754 2104 1189
Size: 37 528 350 4169
Fragments arranged by size:
4169 528 350 37
BamHI G'GATC_C
Cuts at: 2109 2109
Size: 5084
BanI G'GyrC_C
Cuts at: 2100 2514 2762 2797 2100
Size: 414 248 35 4387
Fragments arranged by size:
4387 414 248 35
BanII G_rGCy'C
Cuts at: 737 2098 3128 737
Size: 1361 1030 2693
Fragments arranged by size:
2693 1361 1030
BbeI G_GCGC'C
Cuts at: 2518 2766 2518
Size: 248 4836
BbsI GAAGACnn'nnnn_
Cuts at: 223 223
Size: 5084
BciVI GTATCCnnnnn_n'
Cuts at: 1196 2982 4038 5084 1196
Size: 1786 1056 1046 1196
Fragments arranged by size:
1786 1196 1056 1046
BclI T'GATC_A
Cuts at: 2604 2604
Size: 5084
BfrBI ATG'CAT
Cuts at: 1476 1476
Size: 5084
BglII A'GATC_T
Cuts at: 328 2599 4636 328
Size: 2271 2037 776
Fragments arranged by size:
2271 2037 776
BlpI GC'TnA_GC
Cuts at: 2196 2196
Size: 5084
Bme1580I G_kGCm'C
Cuts at: 2709 2802 3926 2709
Size: 93 1124 3867
Fragments arranged by size:
3867 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 598 2296 2377 2712 4856 598
Size: 1698 81 335 2144 826
Fragments arranged by size:
2144 1698 826 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3451 3451
Size: 5084
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 482 482
Size: 5084
Bpu10I CC'TnA_GC
Cuts at: 131 131
Size: 5084
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2260 3139 3853 4151 2260
Size: 879 714 298 3193
Fragments arranged by size:
3193 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1305 4788 1305
Size: 3483 1601
BsaAI yAC'GTr
Cuts at: 2437 2437
Size: 5084
BsaBI GATnn'nnATC
Cuts at: 6 2598 4635 6
Size: 2592 2037 455
Fragments arranged by size:
2592 2037 455
BsaHI Gr'CG_yC
Cuts at: 2515 2763 3465 4961 2515
Size: 248 702 1496 2638
Fragments arranged by size:
2638 1496 702 248
BsaWI w'CCGG_w
Cuts at: 471 2273 2794 3472 3883 4030 4304 471
Size: 1802 521 678 411 147 274 1251
Fragments arranged by size:
1802 1251 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 480 510 1365 1395 480
Size: 30 855 30 4169
Fragments arranged by size:
4169 855 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 152 1373 152
Size: 1221 3863
BseYI C'CCAG_C
Cuts at: 2509 3267 3932 4340 2509
Size: 758 665 408 3253
Fragments arranged by size:
3253 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 169 169
Size: 5084
BsiEI CG_ry'CG
Cuts at: 104 2626 2672 3902 104
Size: 2522 46 1230 1286
Fragments arranged by size:
2522 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 737 971 2098 2876 3066 3926 737
Size: 234 1127 778 190 860 1895
Fragments arranged by size:
1895 1127 860 778 234 190
BsmI GAATG_Cn'
Cuts at: 1056 1056
Size: 5084
Bsp1286I G_dGCh'C
Cuts at: 737 971 2098 2709 2802 2876 3066 3128 3926
Size: 234 1127 611 93 74 190 62 798
Cuts at: 3926 737
Size: 1895
Fragments arranged by size:
1895 1127 798 611 234 190 93 74
62
BspHI T'CATG_A
Cuts at: 3516 3516
Size: 5084
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2134 2348 2650 3031 2134
Size: 214 302 381 4187
Fragments arranged by size:
4187 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1325 3376 3430 4914 5079 50
Size: 1275 2051 54 1484 165 55
Fragments arranged by size:
2051 1484 1275 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1125 2996 1125
Size: 1871 3213
BsrFI r'CCGG_y
Cuts at: 3082 3263 3082
Size: 181 4903
BssSI C'ACGA_G
Cuts at: 772 3355 4063 772
Size: 2583 708 1793
Fragments arranged by size:
2583 1793 708
BstAPI GCAn_nnn'nTGC
Cuts at: 2461 2461
Size: 5084
BstBI TT'CG_AA
Cuts at: 3445 3445
Size: 5084
BstXI CCAn_nnnn'nTGG
Cuts at: 114 908 114
Size: 794 4290
BstYI r'GATC_y
Cuts at: 1 328 2109 2576 2599 2934 3180 3486 3498
Size: 327 1781 467 23 335 246 306 12
Cuts at: 3498 3584 4636 1
Size: 86 1052 449
Fragments arranged by size:
1781 1052 467 449 335 327 306 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 265 265
Size: 5084
BtgI C'CryG_G
Cuts at: 107 386 107
Size: 279 4805
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3034 3215 3378 3034
Size: 181 163 4740
Fragments arranged by size:
4740 181 163
BtsI GCAGTG_nn'
Cuts at: 1950 2327 1950
Size: 377 4707
DraI TTT'AAA
Cuts at: 1572 2083 1572
Size: 511 4573
DraIII CAC_nnn'GTG
Cuts at: 1363 1363
Size: 5084
DrdI GACnn_nn'nnGTC
Cuts at: 1756 2790 4134 1756
Size: 1034 1344 2706
Fragments arranged by size:
2706 1344 1034
EaeI y'GGCC_r
Cuts at: 1665 1871 2669 2843 3234 3261 4606 1665
Size: 206 798 174 391 27 1345 2143
Fragments arranged by size:
2143 1345 798 391 206 174 27
EagI C'GGCC_G
Cuts at: 2669 2669
Size: 5084
EarI CTCTTCn'nnn_
Cuts at: 299 749 1324 3107 3317 299
Size: 450 575 1783 210 2066
Fragments arranged by size:
2066 1783 575 450 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3292 4034 4180 4894 3292
Size: 742 146 714 3482
Fragments arranged by size:
3482 742 714 146
EcoICRI GAG'CTC
Cuts at: 735 2096 735
Size: 1361 3723
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 482 512 1275 1424 2909 3341 3694 4356 482
Size: 30 763 149 1485 432 353 662 1210
Fragments arranged by size:
1485 1210 763 662 432 353 149 30
EcoO109I rG'GnC_Cy
Cuts at: 1922 2223 1922
Size: 301 4783
EcoRI G'AATT_C
Cuts at: 2088 2088
Size: 5084
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3103 3103
Size: 5084
FspI TGC'GCA
Cuts at: 2865 2865
Size: 5084
HaeII r_GCGC'y
Cuts at: 2518 2766 3996 4767 2518
Size: 248 1230 771 2835
Fragments arranged by size:
2835 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 915 947 1170 1202 2926 2956 2958 2988 915
Size: 32 223 32 1724 30 2 30 3011
Fragments arranged by size:
3011 1724 223 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1125 2123 1125
Size: 998 4086
KasI G'GCGC_C
Cuts at: 2514 2762 2514
Size: 248 4836
KpnI G_GTAC'C
Cuts at: 2104 2104
Size: 5084
MluI A'CGCG_T
Cuts at: 4517 4517
Size: 5084
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3843 4027 3843
Size: 184 4900
MscI TGG'CCA
Cuts at: 1873 2845 1873
Size: 972 4112
MslI CAynn'nnrTG
Cuts at: 112 1060 3073 3200 112
Size: 948 2013 127 1996
Fragments arranged by size:
2013 1996 948 127
MspA1I CmG'CkG
Cuts at: 2195 2509 2869 3653 3898 2195
Size: 314 360 784 245 3381
Fragments arranged by size:
3381 784 360 314 245
NaeI GCC'GGC
Cuts at: 3265 3265
Size: 5084
NarI GG'CG_CC
Cuts at: 2515 2763 2515
Size: 248 4836
NcoI C'CATG_G
Cuts at: 107 386 107
Size: 279 4805
NgoMIV G'CCGG_C
Cuts at: 3263 3263
Size: 5084
NsiI A_TGCA'T
Cuts at: 1478 1478
Size: 5084
NspI r_CATG'y
Cuts at: 1774 2137 2637 4240 1774
Size: 363 500 1603 2618
Fragments arranged by size:
2618 1603 500 363
PciI A'CATG_T
Cuts at: 4236 4236
Size: 5084
PflMI CCAn_nnn'nTGG
Cuts at: 1719 1719
Size: 5084
PmeI GTTT'AAAC
Cuts at: 2083 2083
Size: 5084
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3530 3562 3530
Size: 32 5052
PspXI vC'TCGA_Gb
Cuts at: 1754 1754
Size: 5084
PstI C_TGCA'G
Cuts at: 1515 2131 2816 1515
Size: 616 685 3783
Fragments arranged by size:
3783 685 616
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5084
PvuII CAG'CTG
Cuts at: 2509 2869 2509
Size: 360 4724
RsrII CG'GwC_CG
Cuts at: 3279 3279
Size: 5084
SacI G_AGCT'C
Cuts at: 737 2098 737
Size: 1361 3723
SalI G'TCGA_C
Cuts at: 2121 2121
Size: 5084
SapI GCTCTTCn'nnn_
Cuts at: 3107 3317 3107
Size: 210 4874
SbfI CC_TGCA'GG
Cuts at: 2131 2131
Size: 5084
ScaI AGT'ACT
Cuts at: 729 4601 729
Size: 3872 1212
SfcI C'TryA_G
Cuts at: 33 1158 1511 2127 2812 3780 3971 33
Size: 1125 353 616 685 968 191 1146
Fragments arranged by size:
1146 1125 968 685 616 353 191
SfoI GGC'GCC
Cuts at: 2516 2764 2516
Size: 248 4836
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5084
SmaI CCC'GGG
Cuts at: 2106 2106
Size: 5084
SmlI C'TyrA_G
Cuts at: 1754 2239 3154 3868 4130 1754
Size: 485 915 714 262 2708
Fragments arranged by size:
2708 915 714 485 262
SpeI A'CTAG_T
Cuts at: 67 625 67
Size: 558 4526
SphI G_CATG'C
Cuts at: 1774 2137 2637 1774
Size: 363 500 4221
Fragments arranged by size:
4221 500 363
SspI AAT'ATT
Cuts at: 284 284
Size: 5084
StyI C'CwwG_G
Cuts at: 107 386 2218 2572 107
Size: 279 1832 354 2619
Fragments arranged by size:
2619 1832 354 279
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 617 2667 2800 3259 3468 617
Size: 2050 133 459 209 2233
Fragments arranged by size:
2233 2050 459 209 133
TatI w'GTAC_w
Cuts at: 343 727 4599 343
Size: 384 3872 828
Fragments arranged by size:
3872 828 384
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 480 512 1365 1397 4601 4633 480
Size: 32 853 32 3204 32 931
Fragments arranged by size:
3204 931 853 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 646 2881 646
Size: 2235 2849
XbaI T'CTAG_A
Cuts at: 297 2115 297
Size: 1818 3266
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2579 2579
Size: 5084
XhoI C'TCGA_G
Cuts at: 1754 1754
Size: 5084
XmaI C'CCGG_G
Cuts at: 2104 2104
Size: 5084
XmnI GAAnn'nnTTC
Cuts at: 1153 1153
Size: 5084
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BciVI BclI BfrBI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NsiI NspI PciI PflMI PmeI PpiI PspXI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
AloI ApaI AscI AvrII BaeI BbvCI BcgI BglI
BmgBI BmtI BsiWI BsmBI BspEI BsrGI BssHII BstEII
BstZ17I ClaI CspCI EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI PacI PasI
PfoI PmlI PpuMI PshAI PsiI PspOMI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI StuI SwaI
ZraI