Restriction map for
pF1KB8675
(Circular) (Six-base) MAPSORT of: pF1KB8675.seq Check: 6251 from: 1 to: 4979
pF1KB8675 4979 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 390 899 390
Size: 509 4470
AatII G_ACGT'C
Cuts at: 584 802 584
Size: 218 4761
AccI GT'mk_AC
Cuts at: 1436 2017 1436
Size: 581 4398
Acc65I G'GTAC_C
Cuts at: 1995 1995
Size: 4979
AclI AA'CG_TT
Cuts at: 4819 4819
Size: 4979
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1258 2804 3236 3589 4251 1258
Size: 1546 432 353 662 1986
Fragments arranged by size:
1986 1546 662 432 353
AfeI AGC'GCT
Cuts at: 277 277
Size: 4979
AflIII A'CryG_T
Cuts at: 2331 4131 4412 2331
Size: 1800 281 2898
Fragments arranged by size:
2898 1800 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 304 1798 304
Size: 1494 3485
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 592 624 592
Size: 32 4947
AlwNI CAG_nnn'CTG
Cuts at: 189 390 432 465 2284 3722 189
Size: 201 42 33 1819 1438 1446
Fragments arranged by size:
1819 1446 1438 201 42 33
ApaI G_GGCC'C
Cuts at: 1187 1479 1187
Size: 292 4687
ApaLI G'TGCA_C
Cuts at: 3817 3817
Size: 4979
ApoI r'AATT_y
Cuts at: 1983 4332 4588 1983
Size: 2349 256 2374
Fragments arranged by size:
2374 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4979
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4979
AvaI C'yCGr_G
Cuts at: 496 1180 1675 1999 496
Size: 684 495 324 3476
Fragments arranged by size:
3476 684 495 324
BamHI G'GATC_C
Cuts at: 2004 2004
Size: 4979
BanI G'GyrC_C
Cuts at: 166 1487 1648 1807 1995 2409 2657 2692 166
Size: 1321 161 159 188 414 248 35 2453
Fragments arranged by size:
2453 1321 414 248 188 161 159 35
BanII G_rGCy'C
Cuts at: 634 1187 1479 1823 1993 3023 634
Size: 553 292 344 170 1030 2590
Fragments arranged by size:
2590 1030 553 344 292 170
BbeI G_GCGC'C
Cuts at: 1491 2413 2661 1491
Size: 922 248 3809
Fragments arranged by size:
3809 922 248
BbsI GAAGACnn'nnnn_
Cuts at: 557 791 802 557
Size: 234 11 4734
Fragments arranged by size:
4734 234 11
BbvCI CC'TCA_GC
Cuts at: 1127 1127
Size: 4979
BciVI GTATCCnnnnn_n'
Cuts at: 2877 3933 4979 2877
Size: 1056 1046 2877
Fragments arranged by size:
2877 1056 1046
BclI T'GATC_A
Cuts at: 2499 2499
Size: 4979
BglI GCCn_nnn'nGGC
Cuts at: 223 223
Size: 4979
BglII A'GATC_T
Cuts at: 1163 2494 4531 1163
Size: 1331 2037 1611
Fragments arranged by size:
2037 1611 1331
BlpI GC'TnA_GC
Cuts at: 142 1010 1087 2091 142
Size: 868 77 1004 3030
Fragments arranged by size:
3030 1004 868 77
Bme1580I G_kGCm'C
Cuts at: 169 219 1032 1158 1187 1479 1810 2604 2697
Size: 50 813 126 29 292 331 794 93
Cuts at: 2697 3821 169
Size: 1124 1327
Fragments arranged by size:
1327 1124 813 794 331 292 126 93
50 29
BmgBI CAC'GTC
Cuts at: 1683 1683
Size: 4979
BmrI ACTGGGnnnn_n'
Cuts at: 937 2191 2272 2607 4751 937
Size: 1254 81 335 2144 1165
Fragments arranged by size:
2144 1254 1165 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 282 1329 3346 282
Size: 1047 2017 1915
Fragments arranged by size:
2017 1915 1047
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 211 811 1054 1180 1916 1917 211
Size: 600 243 126 736 1 3273
Fragments arranged by size:
3273 736 600 243 126 1
Bpu10I CC'TnA_GC
Cuts at: 1127 1127
Size: 4979
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1746 2155 3034 3748 4046 1746
Size: 409 879 714 298 2679
Fragments arranged by size:
2679 879 714 409 298
BsaI GGTCTCn'nnnn_
Cuts at: 1258 1610 1912 4683 1258
Size: 352 302 2771 1554
Fragments arranged by size:
2771 1554 352 302
BsaAI yAC'GTr
Cuts at: 2332 2332
Size: 4979
BsaBI GATnn'nnATC
Cuts at: 6 2493 4530 6
Size: 2487 2037 455
Fragments arranged by size:
2487 2037 455
BsaHI Gr'CG_yC
Cuts at: 408 554 581 799 1488 2410 2658 3360 4856
Size: 146 27 218 689 922 248 702 1496
Cuts at: 4856 408
Size: 531
Fragments arranged by size:
1496 922 702 689 531 248 218 146
27
BsaWI w'CCGG_w
Cuts at: 2168 2689 3367 3778 3925 4199 2168
Size: 521 678 411 147 274 2948
Fragments arranged by size:
2948 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 412 442 644 674 784 814 1255 1285 412
Size: 30 202 30 110 30 441 30 4106
Fragments arranged by size:
4106 441 202 110 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 148 218 454 514 517 583 665 1144 1369
Size: 70 236 60 3 66 82 479 225
Cuts at: 1369 148
Size: 3758
Fragments arranged by size:
3758 479 236 225 82 70 66 60
3
BseYI C'CCAG_C
Cuts at: 162 321 491 633 942 1642 1889 2404 3162
Size: 159 170 142 309 700 247 515 758
Cuts at: 3162 3827 4235 162
Size: 665 408 906
Fragments arranged by size:
906 758 700 665 515 408 309 247
170 159 142
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 436 694 436
Size: 258 4721
BsiEI CG_ry'CG
Cuts at: 104 2521 2567 3797 104
Size: 2417 46 1230 1286
Fragments arranged by size:
2417 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1993 2771 2961 3821 1993
Size: 778 190 860 3151
Fragments arranged by size:
3151 860 778 190
Bsp1286I G_dGCh'C
Cuts at: 169 219 634 1032 1158 1187 1479 1810 1823
Size: 50 415 398 126 29 292 331 13
Cuts at: 1823 1993 2604 2697 2771 2961 3023 3821 169
Size: 170 611 93 74 190 62 798 1327
Fragments arranged by size:
1327 798 611 415 398 331 292 190
170 126 93 74 62 50 29 13
BspHI T'CATG_A
Cuts at: 524 1296 3411 524
Size: 772 2115 2092
Fragments arranged by size:
2115 2092 772
BspMI ACCTGCnnnn'nnnn_
Cuts at: 390 899 2029 2243 2545 2926 390
Size: 509 1130 214 302 381 2443
Fragments arranged by size:
2443 1130 509 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1359 3271 3325 4809 4974 50
Size: 1309 1912 54 1484 165 55
Fragments arranged by size:
1912 1484 1309 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2891 2891
Size: 4979
BsrFI r'CCGG_y
Cuts at: 247 1484 2977 3158 247
Size: 1237 1493 181 2068
Fragments arranged by size:
2068 1493 1237 181
BsrGI T'GTAC_A
Cuts at: 1271 1271
Size: 4979
BssSI C'ACGA_G
Cuts at: 3250 3958 3250
Size: 708 4271
BstAPI GCAn_nnn'nTGC
Cuts at: 390 465 516 2356 390
Size: 75 51 1840 3013
Fragments arranged by size:
3013 1840 75 51
BstBI TT'CG_AA
Cuts at: 3340 3340
Size: 4979
BstXI CCAn_nnnn'nTGG
Cuts at: 1941 1941
Size: 4979
BstYI r'GATC_y
Cuts at: 1 533 686 1037 1163 1280 2004 2471 2494
Size: 532 153 351 126 117 724 467 23
Cuts at: 2494 2829 3075 3381 3393 3479 4531 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
1052 724 532 467 449 351 335 306
246 153 126 117 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 242 1765 242
Size: 1523 3456
BtgI C'CryG_G
Cuts at: 107 127 485 947 1940 107
Size: 20 358 462 993 3146
Fragments arranged by size:
3146 993 462 358 20
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2929 3110 3273 2929
Size: 181 163 4635
Fragments arranged by size:
4635 181 163
BtsI GCAGTG_nn'
Cuts at: 480 1608 1722 1782 2222 480
Size: 1128 114 60 440 3237
Fragments arranged by size:
3237 1128 440 114 60
DraI TTT'AAA
Cuts at: 1978 1978
Size: 4979
DrdI GACnn_nn'nnGTC
Cuts at: 2685 4029 2685
Size: 1344 3635
EaeI y'GGCC_r
Cuts at: 110 908 1076 1202 2564 2738 3129 3156 4501
Size: 798 168 126 1362 174 391 27 1345
Cuts at: 4501 110
Size: 588
Fragments arranged by size:
1362 1345 798 588 391 174 168 126
27
EagI C'GGCC_G
Cuts at: 2564 2564
Size: 4979
EarI CTCTTCn'nnn_
Cuts at: 1874 3002 3212 1874
Size: 1128 210 3641
Fragments arranged by size:
3641 1128 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3187 3929 4075 4789 3187
Size: 742 146 714 3377
Fragments arranged by size:
3377 742 714 146
EcoICRI GAG'CTC
Cuts at: 1991 1991
Size: 4979
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 211 811 1054 1180 1258 1916 1917 2804 3236
Size: 600 243 126 78 658 1 887 432
Cuts at: 3236 3589 4251 211
Size: 353 662 939
Fragments arranged by size:
939 887 662 658 600 432 353 243
126 78 1
EcoNI CCTnn'n_nnAGG
Cuts at: 644 1240 644
Size: 596 4383
EcoO109I rG'GnC_Cy
Cuts at: 1050 1475 1476 1687 2118 1050
Size: 425 1 211 431 3911
Fragments arranged by size:
3911 431 425 211 1
EcoRI G'AATT_C
Cuts at: 1983 1983
Size: 4979
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 531 2998 531
Size: 2467 2512
FspI TGC'GCA
Cuts at: 1465 2760 1465
Size: 1295 3684
HaeII r_GCGC'y
Cuts at: 279 1383 1394 1491 2413 2661 3891 4662 279
Size: 1104 11 97 922 248 1230 771 596
Fragments arranged by size:
1230 1104 922 771 596 248 97 11
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 784 816 2821 2851 2853 2883 784
Size: 32 2005 30 2 30 2880
Fragments arranged by size:
2880 2005 32 30 30 2
HincII GTy'rAC
Cuts at: 1452 2018 1452
Size: 566 4413
KasI G'GCGC_C
Cuts at: 1487 2409 2657 1487
Size: 922 248 3809
Fragments arranged by size:
3809 922 248
KpnI G_GTAC'C
Cuts at: 1999 1999
Size: 4979
MluI A'CGCG_T
Cuts at: 4412 4412
Size: 4979
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 524 3738 3922 524
Size: 3214 184 1581
Fragments arranged by size:
3214 1581 184
MscI TGG'CCA
Cuts at: 112 910 1078 1204 2740 112
Size: 798 168 126 1536 2351
Fragments arranged by size:
2351 1536 798 168 126
MslI CAynn'nnrTG
Cuts at: 112 118 1342 2968 3095 112
Size: 6 1224 1626 127 1996
Fragments arranged by size:
1996 1626 1224 127 6
MspA1I CmG'CkG
Cuts at: 129 1415 1696 1736 2090 2404 2764 3548 3793
Size: 1286 281 40 354 314 360 784 245
Cuts at: 3793 129
Size: 1315
Fragments arranged by size:
1315 1286 784 360 354 314 281 245
40
NaeI GCC'GGC
Cuts at: 249 3160 249
Size: 2911 2068
NarI GG'CG_CC
Cuts at: 1488 2410 2658 1488
Size: 922 248 3809
Fragments arranged by size:
3809 922 248
NcoI C'CATG_G
Cuts at: 107 1940 107
Size: 1833 3146
NgoMIV G'CCGG_C
Cuts at: 247 3158 247
Size: 2911 2068
NspI r_CATG'y
Cuts at: 2032 2532 4135 2032
Size: 500 1603 2876
Fragments arranged by size:
2876 1603 500
PciI A'CATG_T
Cuts at: 4131 4131
Size: 4979
PflMI CCAn_nnn'nTGG
Cuts at: 189 189
Size: 4979
PfoI T'CCnGG_A
Cuts at: 914 1040 1754 914
Size: 126 714 4139
Fragments arranged by size:
4139 714 126
PmeI GTTT'AAAC
Cuts at: 1978 1978
Size: 4979
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3425 3457 3425
Size: 32 4947
PpuMI rG'GwC_Cy
Cuts at: 1050 1687 1050
Size: 637 4342
PspOMI G'GGCC_C
Cuts at: 1183 1475 1183
Size: 292 4687
PspXI vC'TCGA_Gb
Cuts at: 496 1675 496
Size: 1179 3800
PstI C_TGCA'G
Cuts at: 470 1802 2026 2711 470
Size: 1332 224 685 2738
Fragments arranged by size:
2738 1332 685 224
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4979
PvuII CAG'CTG
Cuts at: 1415 1696 2404 2764 1415
Size: 281 708 360 3630
Fragments arranged by size:
3630 708 360 281
RsrII CG'GwC_CG
Cuts at: 3174 3174
Size: 4979
SacI G_AGCT'C
Cuts at: 1993 1993
Size: 4979
SacII CC_GC'GG
Cuts at: 130 130
Size: 4979
SalI G'TCGA_C
Cuts at: 2016 2016
Size: 4979
SapI GCTCTTCn'nnn_
Cuts at: 1874 3002 3212 1874
Size: 1128 210 3641
Fragments arranged by size:
3641 1128 210
SbfI CC_TGCA'GG
Cuts at: 2026 2026
Size: 4979
ScaI AGT'ACT
Cuts at: 4496 4496
Size: 4979
SexAI A'CCwGG_T
Cuts at: 716 716
Size: 4979
SfcI C'TryA_G
Cuts at: 33 368 466 1691 1798 2022 2707 3675 3866
Size: 335 98 1225 107 224 685 968 191
Cuts at: 3866 33
Size: 1146
Fragments arranged by size:
1225 1146 968 685 335 224 191 107
98
SfoI GGC'GCC
Cuts at: 1489 2411 2659 1489
Size: 922 248 3809
Fragments arranged by size:
3809 922 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4979
SmaI CCC'GGG
Cuts at: 2001 2001
Size: 4979
SmlI C'TyrA_G
Cuts at: 496 1675 1725 2134 3049 3763 4025 496
Size: 1179 50 409 915 714 262 1450
Fragments arranged by size:
1450 1179 915 714 409 262 50
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4979
SphI G_CATG'C
Cuts at: 2032 2532 2032
Size: 500 4479
SspI AAT'ATT
Cuts at: 1141 1141
Size: 4979
StuI AGG'CCT
Cuts at: 1426 1426
Size: 4979
StyI C'CwwG_G
Cuts at: 107 170 1574 1940 2113 2467 107
Size: 63 1404 366 173 354 2619
Fragments arranged by size:
2619 1404 366 354 173 63
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 956 1649 2562 2695 3154 3363 956
Size: 693 913 133 459 209 2572
Fragments arranged by size:
2572 913 693 459 209 133
TatI w'GTAC_w
Cuts at: 872 998 1271 4494 872
Size: 126 273 3223 1357
Fragments arranged by size:
3223 1357 273 126
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 412 444 644 676 1022 1054 4496 4528 412
Size: 32 200 32 346 32 3442 32 863
Fragments arranged by size:
3442 863 346 200 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2776 2776
Size: 4979
XbaI T'CTAG_A
Cuts at: 2010 2010
Size: 4979
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 594 1218 2474 114
Size: 480 624 1256 2619
Fragments arranged by size:
2619 1256 624 480
XhoI C'TCGA_G
Cuts at: 496 1675 496
Size: 1179 3800
XmaI C'CCGG_G
Cuts at: 1999 1999
Size: 4979
ZraI GAC'GTC
Cuts at: 582 800 582
Size: 218 4761
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PflMI PfoI PmeI PpiI PpuMI
PspOMI PspXI PstI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AflII AgeI AhdI AleI AscI AvrII BaeI BcgI
BfrBI BmtI BsiWI BsmI BsmBI BspEI BssHII BstEII
BstZ17I ClaI CspCI DraIII EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PmlI PshAI PsiI PsrI SanDI SfiI SgrAI
SnaBI SrfI SwaI XmnI