Restriction map for
pF1KB8676
(Circular) (Six-base) MAPSORT of: pF1KB8676.seq Check: 1960 from: 1 to: 4379
pF1KB8676 4379 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 417 417
Size: 4379
AccI GT'mk_AC
Cuts at: 1417 1417
Size: 4379
Acc65I G'GTAC_C
Cuts at: 1305 1395 1305
Size: 90 4289
AclI AA'CG_TT
Cuts at: 4219 4219
Size: 4379
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 470 1112 2204 2636 2989 3651 470
Size: 642 1092 432 353 662 1198
Fragments arranged by size:
1198 1092 662 642 432 353
AflII C'TTAA_G
Cuts at: 367 367
Size: 4379
AflIII A'CryG_T
Cuts at: 1731 3531 3812 1731
Size: 1800 281 2298
Fragments arranged by size:
2298 1800 281
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 986 1018 986
Size: 32 4347
AlwNI CAG_nnn'CTG
Cuts at: 1157 1684 3122 1157
Size: 527 1438 2414
Fragments arranged by size:
2414 1438 527
ApaI G_GGCC'C
Cuts at: 488 488
Size: 4379
ApaLI G'TGCA_C
Cuts at: 3217 3217
Size: 4379
ApoI r'AATT_y
Cuts at: 826 1383 3732 3988 826
Size: 557 2349 256 1217
Fragments arranged by size:
2349 1217 557 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4379
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4379
AvaI C'yCGr_G
Cuts at: 941 1399 941
Size: 458 3921
AvrII C'CTAG_G
Cuts at: 316 316
Size: 4379
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1069 1102 1069
Size: 33 4346
BamHI G'GATC_C
Cuts at: 1046 1404 1046
Size: 358 4021
BanI G'GyrC_C
Cuts at: 429 1305 1395 1809 2057 2092 429
Size: 876 90 414 248 35 2716
Fragments arranged by size:
2716 876 414 248 90 35
BanII G_rGCy'C
Cuts at: 488 572 1393 2423 488
Size: 84 821 1030 2444
Fragments arranged by size:
2444 1030 821 84
BbeI G_GCGC'C
Cuts at: 1813 2061 1813
Size: 248 4131
BbsI GAAGACnn'nnnn_
Cuts at: 754 754
Size: 4379
BciVI GTATCCnnnnn_n'
Cuts at: 1097 2277 3333 4379 1097
Size: 1180 1056 1046 1097
Fragments arranged by size:
1180 1097 1056 1046
BclI T'GATC_A
Cuts at: 1899 1899
Size: 4379
BglII A'GATC_T
Cuts at: 1894 3931 1894
Size: 2037 2342
BlpI GC'TnA_GC
Cuts at: 419 902 1491 419
Size: 483 589 3307
Fragments arranged by size:
3307 589 483
Bme1580I G_kGCm'C
Cuts at: 488 2004 2097 3221 488
Size: 1516 93 1124 1646
Fragments arranged by size:
1646 1516 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 521 1145 1591 1672 2007 4151 521
Size: 624 446 81 335 2144 749
Fragments arranged by size:
2144 749 624 446 335 81
BmtI G_CTAG'C
Cuts at: 1019 1019
Size: 4379
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 462 2746 462
Size: 2284 2095
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 478 478
Size: 4379
Bpu10I CC'TnA_GC
Cuts at: 325 325
Size: 4379
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 275 529 961 1555 2434 3148 3446 275
Size: 254 432 594 879 714 298 1208
Fragments arranged by size:
1208 879 714 594 432 298 254
BsaI GGTCTCn'nnnn_
Cuts at: 1002 1140 4083 1002
Size: 138 2943 1298
Fragments arranged by size:
2943 1298 138
BsaAI yAC'GTr
Cuts at: 1732 1732
Size: 4379
BsaBI GATnn'nnATC
Cuts at: 6 1893 3930 6
Size: 1887 2037 455
Fragments arranged by size:
2037 1887 455
BsaHI Gr'CG_yC
Cuts at: 1810 2058 2760 4256 1810
Size: 248 702 1496 1933
Fragments arranged by size:
1933 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1568 2089 2767 3178 3325 3599 1568
Size: 521 678 411 147 274 2348
Fragments arranged by size:
2348 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 110 140 160 190 338 368 986 1016 110
Size: 30 20 30 148 30 618 30 3473
Fragments arranged by size:
3473 618 148 30 30 30 30 20
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 945 945
Size: 4379
BseYI C'CCAG_C
Cuts at: 329 565 1804 2562 3227 3635 329
Size: 236 1239 758 665 408 1073
Fragments arranged by size:
1239 1073 758 665 408 236
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 642 661 1143 642
Size: 19 482 3878
Fragments arranged by size:
3878 482 19
BsiEI CG_ry'CG
Cuts at: 104 1921 1967 3197 104
Size: 1817 46 1230 1286
Fragments arranged by size:
1817 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1080 1393 2171 2361 3221 1080
Size: 313 778 190 860 2238
Fragments arranged by size:
2238 860 778 313 190
BsmI GAATG_Cn'
Cuts at: 139 139
Size: 4379
Bsp1286I G_dGCh'C
Cuts at: 488 572 1080 1393 2004 2097 2171 2361 2423
Size: 84 508 313 611 93 74 190 62
Cuts at: 2423 3221 488
Size: 798 1646
Fragments arranged by size:
1646 798 611 508 313 190 93 84
74 62
BspHI T'CATG_A
Cuts at: 1139 1181 2811 1139
Size: 42 1630 2707
Fragments arranged by size:
2707 1630 42
BspMI ACCTGCnnnn'nnnn_
Cuts at: 417 719 798 1020 1429 1643 1945 2326 417
Size: 302 79 222 409 214 302 381 2470
Fragments arranged by size:
2470 409 381 302 302 222 214 79
BsrBI CCG'CTC
Cuts at: 50 2671 2725 4209 4374 50
Size: 2621 54 1484 165 55
Fragments arranged by size:
2621 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 296 2291 296
Size: 1995 2384
BsrFI r'CCGG_y
Cuts at: 1330 2377 2558 1330
Size: 1047 181 3151
Fragments arranged by size:
3151 1047 181
BsrGI T'GTAC_A
Cuts at: 1172 1172
Size: 4379
BssSI C'ACGA_G
Cuts at: 2650 3358 2650
Size: 708 3671
BstAPI GCAn_nnn'nTGC
Cuts at: 1756 1756
Size: 4379
BstBI TT'CG_AA
Cuts at: 2740 2740
Size: 4379
BstYI r'GATC_y
Cuts at: 1 1046 1404 1871 1894 2229 2475 2781 2793
Size: 1045 358 467 23 335 246 306 12
Cuts at: 2793 2879 3931 1
Size: 86 1052 449
Fragments arranged by size:
1052 1045 467 449 358 335 306 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 380 433 852 107
Size: 273 53 419 3634
Fragments arranged by size:
3634 419 273 53
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2329 2510 2673 2329
Size: 181 163 4035
Fragments arranged by size:
4035 181 163
BtsI GCAGTG_nn'
Cuts at: 477 896 1622 477
Size: 419 726 3234
Fragments arranged by size:
3234 726 419
DraI TTT'AAA
Cuts at: 554 1378 554
Size: 824 3555
DrdI GACnn_nn'nnGTC
Cuts at: 2085 3429 2085
Size: 1344 3035
EaeI y'GGCC_r
Cuts at: 1328 1964 2138 2529 2556 3901 1328
Size: 636 174 391 27 1345 1806
Fragments arranged by size:
1806 1345 636 391 174 27
EagI C'GGCC_G
Cuts at: 1964 1964
Size: 4379
EarI CTCTTCn'nnn_
Cuts at: 1138 1357 2402 2612 1138
Size: 219 1045 210 2905
Fragments arranged by size:
2905 1045 219 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2587 3329 3475 4189 2587
Size: 742 146 714 2777
Fragments arranged by size:
2777 742 714 146
EcoICRI GAG'CTC
Cuts at: 1391 1391
Size: 4379
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 470 478 1112 2204 2636 2989 3651 470
Size: 8 634 1092 432 353 662 1198
Fragments arranged by size:
1198 1092 662 634 432 353 8
EcoO109I rG'GnC_Cy
Cuts at: 321 516 708 810 1518 321
Size: 195 192 102 708 3182
Fragments arranged by size:
3182 708 195 192 102
EcoRI G'AATT_C
Cuts at: 826 1383 826
Size: 557 3822
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2398 2398
Size: 4379
FspI TGC'GCA
Cuts at: 2160 2160
Size: 4379
HaeII r_GCGC'y
Cuts at: 823 1200 1813 2061 3291 4062 823
Size: 377 613 248 1230 771 1140
Fragments arranged by size:
1230 1140 771 613 377 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 935 967 1016 1021 1048 1053 1085 1204 1236
Size: 32 49 5 27 5 32 119 32
Cuts at: 1236 2221 2251 2253 2283 935
Size: 985 30 2 30 3031
Fragments arranged by size:
3031 985 119 49 32 32 32 30
30 27 5 5 2
HincII GTy'rAC
Cuts at: 1418 1418
Size: 4379
KasI G'GCGC_C
Cuts at: 1809 2057 1809
Size: 248 4131
KpnI G_GTAC'C
Cuts at: 1309 1399 1309
Size: 90 4289
MluI A'CGCG_T
Cuts at: 3812 3812
Size: 4379
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 668 3138 3322 668
Size: 2470 184 1725
Fragments arranged by size:
2470 1725 184
MscI TGG'CCA
Cuts at: 2140 2140
Size: 4379
MslI CAynn'nnrTG
Cuts at: 112 857 1339 2368 2495 112
Size: 745 482 1029 127 1996
Fragments arranged by size:
1996 1029 745 482 127
MspA1I CmG'CkG
Cuts at: 435 565 645 1324 1490 1804 2164 2948 3193
Size: 130 80 679 166 314 360 784 245
Cuts at: 3193 435
Size: 1621
Fragments arranged by size:
1621 784 679 360 314 245 166 130
80
NaeI GCC'GGC
Cuts at: 1332 2560 1332
Size: 1228 3151
NarI GG'CG_CC
Cuts at: 1810 2058 1810
Size: 248 4131
NcoI C'CATG_G
Cuts at: 107 852 107
Size: 745 3634
NgoMIV G'CCGG_C
Cuts at: 1330 2558 1330
Size: 1228 3151
NheI G'CTAG_C
Cuts at: 1015 1015
Size: 4379
NspI r_CATG'y
Cuts at: 1371 1432 1932 3535 1371
Size: 61 500 1603 2215
Fragments arranged by size:
2215 1603 500 61
PasI CC'CwG_GG
Cuts at: 702 702
Size: 4379
PciI A'CATG_T
Cuts at: 3531 3531
Size: 4379
PfoI T'CCnGG_A
Cuts at: 128 164 128
Size: 36 4343
PmeI GTTT'AAAC
Cuts at: 1378 1378
Size: 4379
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 986 1018 2825 2857 986
Size: 32 1807 32 2508
Fragments arranged by size:
2508 1807 32 32
PpuMI rG'GwC_Cy
Cuts at: 321 516 708 810 321
Size: 195 192 102 3890
Fragments arranged by size:
3890 195 192 102
PspOMI G'GGCC_C
Cuts at: 484 484
Size: 4379
PstI C_TGCA'G
Cuts at: 428 836 1269 1426 2111 428
Size: 408 433 157 685 2696
Fragments arranged by size:
2696 685 433 408 157
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4379
PvuII CAG'CTG
Cuts at: 565 645 1324 1804 2164 565
Size: 80 679 480 360 2780
Fragments arranged by size:
2780 679 480 360 80
RsrII CG'GwC_CG
Cuts at: 2574 2574
Size: 4379
SacI G_AGCT'C
Cuts at: 1393 1393
Size: 4379
SacII CC_GC'GG
Cuts at: 436 436
Size: 4379
SalI G'TCGA_C
Cuts at: 1416 1416
Size: 4379
SanDI GG'GwC_CC
Cuts at: 516 516
Size: 4379
SapI GCTCTTCn'nnn_
Cuts at: 2402 2612 2402
Size: 210 4169
SbfI CC_TGCA'GG
Cuts at: 836 1426 836
Size: 590 3789
ScaI AGT'ACT
Cuts at: 3896 3896
Size: 4379
SexAI A'CCwGG_T
Cuts at: 635 635
Size: 4379
SfcI C'TryA_G
Cuts at: 33 424 832 1265 1362 1422 2107 3075 3266
Size: 391 408 433 97 60 685 968 191
Cuts at: 3266 33
Size: 1146
Fragments arranged by size:
1146 968 685 433 408 391 191 97
60
SfoI GGC'GCC
Cuts at: 1811 2059 1811
Size: 248 4131
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4379
SmaI CCC'GGG
Cuts at: 1401 1401
Size: 4379
SmlI C'TyrA_G
Cuts at: 290 367 544 976 1534 2449 3163 3425 290
Size: 77 177 432 558 915 714 262 1244
Fragments arranged by size:
1244 915 714 558 432 262 177 77
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4379
SphI G_CATG'C
Cuts at: 1371 1432 1932 1371
Size: 61 500 3818
Fragments arranged by size:
3818 500 61
SspI AAT'ATT
Cuts at: 876 876
Size: 4379
StuI AGG'CCT
Cuts at: 920 920
Size: 4379
StyI C'CwwG_G
Cuts at: 107 316 852 1513 1867 107
Size: 209 536 661 354 2619
Fragments arranged by size:
2619 661 536 354 209
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1962 2095 2554 2763 1962
Size: 133 459 209 3578
Fragments arranged by size:
3578 459 209 133
TatI w'GTAC_w
Cuts at: 1172 3894 1172
Size: 2722 1657
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 110 142 338 370 3896 3928 110
Size: 32 196 32 3526 32 561
Fragments arranged by size:
3526 561 196 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2176 2176
Size: 4379
XbaI T'CTAG_A
Cuts at: 1410 1410
Size: 4379
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 411 1874 411
Size: 1463 2916
XmaI C'CCGG_G
Cuts at: 1399 1399
Size: 4379
XmnI GAAnn'nnTTC
Cuts at: 389 998 389
Size: 609 3770
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII
BaeI BamHI BanI BanII BbeI BbsI BciVI BclI
BglII BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NheI NspI PasI
PciI PfoI PmeI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AgeI AhdI AleI AlfI AscI BbvCI
BcgI BfrBI BglI BmgBI BsiWI BsmBI BspEI BssHII
BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NotI
NruI NsiI PacI PflMI PmlI PshAI PsiI PspXI
PsrI SfiI SgrAI SnaBI SrfI SwaI XhoI ZraI