Restriction map for
pF1KB8678
(Circular) (Six-base) MAPSORT of: pF1KB8678.seq Check: 9600 from: 1 to: 4241
pF1KB8678 4241 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AatII G_ACGT'C
Cuts at: 677 677
Size: 4241
AccI GT'mk_AC
Cuts at: 1279 1279
Size: 4241
Acc65I G'GTAC_C
Cuts at: 1257 1257
Size: 4241
AclI AA'CG_TT
Cuts at: 4081 4081
Size: 4241
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 879 2066 2498 2851 3513 879
Size: 1187 432 353 662 1607
Fragments arranged by size:
1607 1187 662 432 353
AflIII A'CryG_T
Cuts at: 1593 3393 3674 1593
Size: 1800 281 2160
Fragments arranged by size:
2160 1800 281
AleI CACnn'nnGTG
Cuts at: 242 571 242
Size: 329 3912
AlwNI CAG_nnn'CTG
Cuts at: 1546 2984 1546
Size: 1438 2803
ApaI G_GGCC'C
Cuts at: 882 882
Size: 4241
ApaLI G'TGCA_C
Cuts at: 3079 3079
Size: 4241
ApoI r'AATT_y
Cuts at: 1245 3594 3850 1245
Size: 2349 256 1636
Fragments arranged by size:
2349 1636 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4241
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4241
AvaI C'yCGr_G
Cuts at: 1261 1261
Size: 4241
BamHI G'GATC_C
Cuts at: 157 1266 157
Size: 1109 3132
BanI G'GyrC_C
Cuts at: 444 1207 1257 1671 1919 1954 444
Size: 763 50 414 248 35 2731
Fragments arranged by size:
2731 763 414 248 50 35
BanII G_rGCy'C
Cuts at: 261 882 1141 1255 2285 261
Size: 621 259 114 1030 2217
Fragments arranged by size:
2217 1030 621 259 114
BbeI G_GCGC'C
Cuts at: 448 1675 1923 448
Size: 1227 248 2766
Fragments arranged by size:
2766 1227 248
BbsI GAAGACnn'nnnn_
Cuts at: 1208 1208
Size: 4241
BbvCI CC'TCA_GC
Cuts at: 293 293
Size: 4241
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 642 676 642
Size: 34 4207
BciVI GTATCCnnnnn_n'
Cuts at: 2139 3195 4241 2139
Size: 1056 1046 2139
Fragments arranged by size:
2139 1056 1046
BclI T'GATC_A
Cuts at: 189 1761 189
Size: 1572 2669
BglII A'GATC_T
Cuts at: 1756 3793 1756
Size: 2037 2204
BlpI GC'TnA_GC
Cuts at: 1353 1353
Size: 4241
Bme1580I G_kGCm'C
Cuts at: 512 614 882 1212 1866 1959 3083 512
Size: 102 268 330 654 93 1124 1670
Fragments arranged by size:
1670 1124 654 330 268 102 93
BmrI ACTGGGnnnn_n'
Cuts at: 1453 1534 1869 4013 1453
Size: 81 335 2144 1681
Fragments arranged by size:
2144 1681 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2608 2608
Size: 4241
Bpu10I CC'TnA_GC
Cuts at: 293 293
Size: 4241
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1417 2296 3010 3308 1417
Size: 879 714 298 2350
Fragments arranged by size:
2350 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 490 1185 3945 490
Size: 695 2760 786
Fragments arranged by size:
2760 786 695
BsaAI yAC'GTr
Cuts at: 1127 1594 1127
Size: 467 3774
BsaBI GATnn'nnATC
Cuts at: 6 1755 3792 6
Size: 1749 2037 455
Fragments arranged by size:
2037 1749 455
BsaHI Gr'CG_yC
Cuts at: 445 674 1672 1920 2622 4118 445
Size: 229 998 248 702 1496 568
Fragments arranged by size:
1496 998 702 568 248 229
BsaWI w'CCGG_w
Cuts at: 160 1430 1951 2629 3040 3187 3461 160
Size: 1270 521 678 411 147 274 940
Fragments arranged by size:
1270 940 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1102 1132 1102
Size: 30 4211
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 310 433 931 310
Size: 123 498 3620
Fragments arranged by size:
3620 498 123
BseYI C'CCAG_C
Cuts at: 1666 2424 3089 3497 1666
Size: 758 665 408 2410
Fragments arranged by size:
2410 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1085 1085
Size: 4241
BsiEI CG_ry'CG
Cuts at: 104 1783 1829 3059 104
Size: 1679 46 1230 1286
Fragments arranged by size:
1679 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 302 434 1255 2033 2223 3083 302
Size: 132 821 778 190 860 1460
Fragments arranged by size:
1460 860 821 778 190 132
BsmBI CGTCTCn'nnnn_
Cuts at: 681 796 681
Size: 115 4126
Bsp1286I G_dGCh'C
Cuts at: 261 302 434 512 614 882 1141 1212 1255
Size: 41 132 78 102 268 259 71 43
Cuts at: 1255 1866 1959 2033 2223 2285 3083 261
Size: 611 93 74 190 62 798 1419
Fragments arranged by size:
1419 798 611 268 259 190 132 102
93 78 74 71 62 43 41
BspHI T'CATG_A
Cuts at: 2673 2673
Size: 4241
BspMI ACCTGCnnnn'nnnn_
Cuts at: 707 842 983 1291 1505 1807 2188 707
Size: 135 141 308 214 302 381 2760
Fragments arranged by size:
2760 381 308 302 214 141 135
BsrBI CCG'CTC
Cuts at: 50 514 2533 2587 4071 4236 50
Size: 464 2019 54 1484 165 55
Fragments arranged by size:
2019 1484 464 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2153 2153
Size: 4241
BsrFI r'CCGG_y
Cuts at: 2239 2420 2239
Size: 181 4060
BssSI C'ACGA_G
Cuts at: 934 2512 3220 934
Size: 1578 708 1955
Fragments arranged by size:
1955 1578 708
BstAPI GCAn_nnn'nTGC
Cuts at: 429 1618 429
Size: 1189 3052
BstBI TT'CG_AA
Cuts at: 2602 2602
Size: 4241
BstXI CCAn_nnnn'nTGG
Cuts at: 573 573
Size: 4241
BstYI r'GATC_y
Cuts at: 1 157 194 1266 1733 1756 2091 2337 2643
Size: 156 37 1072 467 23 335 246 306
Cuts at: 2643 2655 2741 3793 1
Size: 12 86 1052 449
Fragments arranged by size:
1072 1052 467 449 335 306 246 156
86 37 23 12
Bsu36I CC'TnA_GG
Cuts at: 1106 1106
Size: 4241
BtgI C'CryG_G
Cuts at: 107 329 107
Size: 222 4019
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 645 2191 2372 2535 645
Size: 1546 181 163 2351
Fragments arranged by size:
2351 1546 181 163
BtsI GCAGTG_nn'
Cuts at: 1484 1484
Size: 4241
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 203 238 1113 1148 203
Size: 35 875 35 3296
Fragments arranged by size:
3296 875 35 35
DraI TTT'AAA
Cuts at: 452 1240 452
Size: 788 3453
DrdI GACnn_nn'nnGTC
Cuts at: 1947 3291 1947
Size: 1344 2897
EaeI y'GGCC_r
Cuts at: 1826 2000 2391 2418 3763 1826
Size: 174 391 27 1345 2304
Fragments arranged by size:
2304 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1826 1826
Size: 4241
EarI CTCTTCn'nnn_
Cuts at: 898 2264 2474 898
Size: 1366 210 2665
Fragments arranged by size:
2665 1366 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2449 3191 3337 4051 2449
Size: 742 146 714 2639
Fragments arranged by size:
2639 742 714 146
EcoICRI GAG'CTC
Cuts at: 1253 1253
Size: 4241
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 879 2066 2498 2851 3513 879
Size: 1187 432 353 662 1607
Fragments arranged by size:
1607 1187 662 432 353
EcoNI CCTnn'n_nnAGG
Cuts at: 439 703 439
Size: 264 3977
EcoO109I rG'GnC_Cy
Cuts at: 879 939 1380 879
Size: 60 441 3740
Fragments arranged by size:
3740 441 60
EcoRI G'AATT_C
Cuts at: 1245 1245
Size: 4241
EcoRV GAT'ATC
Cuts at: 1097 1097
Size: 4241
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2260 2260
Size: 4241
FspI TGC'GCA
Cuts at: 652 2022 652
Size: 1370 2871
FspAI rTGC'GCAy
Cuts at: 652 652
Size: 4241
HaeII r_GCGC'y
Cuts at: 448 1675 1923 3153 3924 448
Size: 1227 248 1230 771 765
Fragments arranged by size:
1230 1227 771 765 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 142 174 709 741 2083 2113 2115 2145 142
Size: 32 535 32 1342 30 2 30 2238
Fragments arranged by size:
2238 1342 535 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1280 1280
Size: 4241
KasI G'GCGC_C
Cuts at: 444 1671 1919 444
Size: 1227 248 2766
Fragments arranged by size:
2766 1227 248
KpnI G_GTAC'C
Cuts at: 1261 1261
Size: 4241
MluI A'CGCG_T
Cuts at: 3674 3674
Size: 4241
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3000 3184 3000
Size: 184 4057
MscI TGG'CCA
Cuts at: 2002 2002
Size: 4241
MslI CAynn'nnrTG
Cuts at: 242 526 571 2230 2357 242
Size: 284 45 1659 127 2126
Fragments arranged by size:
2126 1659 284 127 45
MspA1I CmG'CkG
Cuts at: 402 1352 1666 2026 2810 3055 402
Size: 950 314 360 784 245 1588
Fragments arranged by size:
1588 950 784 360 314 245
NaeI GCC'GGC
Cuts at: 2422 2422
Size: 4241
NarI GG'CG_CC
Cuts at: 445 1672 1920 445
Size: 1227 248 2766
Fragments arranged by size:
2766 1227 248
NcoI C'CATG_G
Cuts at: 107 329 107
Size: 222 4019
NgoMIV G'CCGG_C
Cuts at: 2420 2420
Size: 4241
NspI r_CATG'y
Cuts at: 1294 1794 3397 1294
Size: 500 1603 2138
Fragments arranged by size:
2138 1603 500
PciI A'CATG_T
Cuts at: 3393 3393
Size: 4241
PmeI GTTT'AAAC
Cuts at: 1240 1240
Size: 4241
PmlI CAC'GTG
Cuts at: 1127 1127
Size: 4241
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2687 2719 2687
Size: 32 4209
PpuMI rG'GwC_Cy
Cuts at: 939 939
Size: 4241
PspOMI G'GGCC_C
Cuts at: 878 878
Size: 4241
PstI C_TGCA'G
Cuts at: 407 1288 1973 407
Size: 881 685 2675
Fragments arranged by size:
2675 881 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4241
PvuII CAG'CTG
Cuts at: 402 1666 2026 402
Size: 1264 360 2617
Fragments arranged by size:
2617 1264 360
RsrII CG'GwC_CG
Cuts at: 2436 2436
Size: 4241
SacI G_AGCT'C
Cuts at: 1255 1255
Size: 4241
SalI G'TCGA_C
Cuts at: 1278 1278
Size: 4241
SapI GCTCTTCn'nnn_
Cuts at: 898 2264 2474 898
Size: 1366 210 2665
Fragments arranged by size:
2665 1366 210
SbfI CC_TGCA'GG
Cuts at: 1288 1288
Size: 4241
ScaI AGT'ACT
Cuts at: 829 3758 829
Size: 2929 1312
SfcI C'TryA_G
Cuts at: 33 403 554 1284 1969 2937 3128 33
Size: 370 151 730 685 968 191 1146
Fragments arranged by size:
1146 968 730 685 370 191 151
SfoI GGC'GCC
Cuts at: 446 1673 1921 446
Size: 1227 248 2766
Fragments arranged by size:
2766 1227 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4241
SmaI CCC'GGG
Cuts at: 1263 1263
Size: 4241
SmlI C'TyrA_G
Cuts at: 1396 2311 3025 3287 1396
Size: 915 714 262 2350
Fragments arranged by size:
2350 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4241
SphI G_CATG'C
Cuts at: 1294 1794 1294
Size: 500 3741
StyI C'CwwG_G
Cuts at: 107 329 1375 1729 107
Size: 222 1046 354 2619
Fragments arranged by size:
2619 1046 354 222
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 616 1824 1957 2416 2625 616
Size: 1208 133 459 209 2232
Fragments arranged by size:
2232 1208 459 209 133
TatI w'GTAC_w
Cuts at: 537 827 3756 537
Size: 290 2929 1022
Fragments arranged by size:
2929 1022 290
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 149 181 3758 3790 149
Size: 32 3577 32 600
Fragments arranged by size:
3577 600 32 32
Tth111I GACn'n_nGTC
Cuts at: 2038 2038
Size: 4241
XbaI T'CTAG_A
Cuts at: 1272 1272
Size: 4241
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1736 1736
Size: 4241
XmaI C'CCGG_G
Cuts at: 1261 1261
Size: 4241
ZraI GAC'GTC
Cuts at: 675 675
Size: 4241
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AleI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglII
BlpI Bme1580I BmrI BplI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
FspAI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PmeI PmlI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AgeI AhdI AlfI AloI AscI
AvrII BaeI BfrBI BglI BmgBI BmtI BpmI BsiWI
BsmI BspEI BsrGI BssHII BstEII BstZ17I ClaI DraIII
FseI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PfoI PshAI PsiI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI