Restriction map for
pF1KB8680
(Circular) (Six-base) MAPSORT of: pF1KB8680.seq Check: 9852 from: 1 to: 5012
pF1KB8680 5012 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AatII G_ACGT'C
Cuts at: 1916 1916
Size: 5012
AccI GT'mk_AC
Cuts at: 983 2050 983
Size: 1067 3945
Acc65I G'GTAC_C
Cuts at: 2028 2028
Size: 5012
AclI AA'CG_TT
Cuts at: 4852 4852
Size: 5012
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 878 1136 1370 1820 2837 3269 3622 4284 878
Size: 258 234 450 1017 432 353 662 1606
Fragments arranged by size:
1606 1017 662 450 432 353 258 234
AflIII A'CryG_T
Cuts at: 1181 1686 2004 2364 4164 4445 1181
Size: 505 318 360 1800 281 1748
Fragments arranged by size:
1800 1748 505 360 318 281
AleI CACnn'nnGTG
Cuts at: 926 926
Size: 5012
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1345 1570 1345
Size: 225 4787
AlwNI CAG_nnn'CTG
Cuts at: 244 2317 3755 244
Size: 2073 1438 1501
Fragments arranged by size:
2073 1501 1438
ApaLI G'TGCA_C
Cuts at: 182 3850 182
Size: 3668 1344
ApoI r'AATT_y
Cuts at: 2016 4365 4621 2016
Size: 2349 256 2407
Fragments arranged by size:
2407 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5012
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5012
AvaI C'yCGr_G
Cuts at: 532 2032 532
Size: 1500 3512
BamHI G'GATC_C
Cuts at: 2037 2037
Size: 5012
BanI G'GyrC_C
Cuts at: 1967 2028 2442 2690 2725 1967
Size: 61 414 248 35 4254
Fragments arranged by size:
4254 414 248 61 35
BanII G_rGCy'C
Cuts at: 533 551 2026 3056 533
Size: 18 1475 1030 2489
Fragments arranged by size:
2489 1475 1030 18
BbeI G_GCGC'C
Cuts at: 2446 2694 2446
Size: 248 4764
BbsI GAAGACnn'nnnn_
Cuts at: 464 980 1523 464
Size: 516 543 3953
Fragments arranged by size:
3953 543 516
BbvCI CC'TCA_GC
Cuts at: 487 542 722 487
Size: 55 180 4777
Fragments arranged by size:
4777 180 55
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4978
BciVI GTATCCnnnnn_n'
Cuts at: 2910 3966 5012 2910
Size: 1056 1046 2910
Fragments arranged by size:
2910 1056 1046
BclI T'GATC_A
Cuts at: 1059 1275 2532 1059
Size: 216 1257 3539
Fragments arranged by size:
3539 1257 216
BfrBI ATG'CAT
Cuts at: 1037 1037
Size: 5012
BglII A'GATC_T
Cuts at: 2527 4564 2527
Size: 2037 2975
BlpI GC'TnA_GC
Cuts at: 760 1318 1543 2124 760
Size: 558 225 581 3648
Fragments arranged by size:
3648 581 558 225
Bme1580I G_kGCm'C
Cuts at: 186 2637 2730 3854 186
Size: 2451 93 1124 1344
Fragments arranged by size:
2451 1344 1124 93
BmgBI CAC'GTC
Cuts at: 1707 1932 1707
Size: 225 4787
BmrI ACTGGGnnnn_n'
Cuts at: 591 2224 2305 2640 4784 591
Size: 1633 81 335 2144 819
Fragments arranged by size:
2144 1633 819 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3379 3379
Size: 5012
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 640 907 640
Size: 267 4745
Bpu10I CC'TnA_GC
Cuts at: 487 542 550 722 487
Size: 55 8 172 4777
Fragments arranged by size:
4777 172 55 8
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 395 2188 3067 3781 4079 395
Size: 1793 879 714 298 1328
Fragments arranged by size:
1793 1328 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4716 4716
Size: 5012
BsaAI yAC'GTr
Cuts at: 2365 2365
Size: 5012
BsaBI GATnn'nnATC
Cuts at: 6 2526 4563 6
Size: 2520 2037 455
Fragments arranged by size:
2520 2037 455
BsaHI Gr'CG_yC
Cuts at: 1913 2443 2691 3393 4889 1913
Size: 530 248 702 1496 2036
Fragments arranged by size:
2036 1496 702 530 248
BsaWI w'CCGG_w
Cuts at: 301 672 2201 2722 3400 3811 3958 4232 301
Size: 371 1529 521 678 411 147 274 1081
Fragments arranged by size:
1529 1081 678 521 411 371 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 689 719 689
Size: 30 4982
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 541 559 655 820 904 1135 541
Size: 18 96 165 84 231 4418
Fragments arranged by size:
4418 231 165 96 84 18
BseYI C'CCAG_C
Cuts at: 353 2437 3195 3860 4268 353
Size: 2084 758 665 408 1097
Fragments arranged by size:
2084 1097 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 868 868
Size: 5012
BsiEI CG_ry'CG
Cuts at: 104 2554 2600 3830 104
Size: 2450 46 1230 1286
Fragments arranged by size:
2450 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 186 533 551 1213 2026 2804 2994 3854 186
Size: 347 18 662 813 778 190 860 1344
Fragments arranged by size:
1344 860 813 778 662 347 190 18
Bsp1286I G_dGCh'C
Cuts at: 186 533 551 1213 2026 2637 2730 2804 2994
Size: 347 18 662 813 611 93 74 190
Cuts at: 2994 3056 3854 186
Size: 62 798 1344
Fragments arranged by size:
1344 813 798 662 611 347 190 93
74 62 18
BspEI T'CCGG_A
Cuts at: 672 672
Size: 5012
BspHI T'CATG_A
Cuts at: 1011 1062 3444 1011
Size: 51 2382 2579
Fragments arranged by size:
2579 2382 51
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2062 2276 2578 2959 2062
Size: 214 302 381 4115
Fragments arranged by size:
4115 381 302 214
BsrBI CCG'CTC
Cuts at: 50 498 638 3304 3358 4842 5007 50
Size: 448 140 2666 54 1484 165 55
Fragments arranged by size:
2666 1484 448 165 140 55 54
BsrDI GCAATG_nn'
Cuts at: 1447 2924 1447
Size: 1477 3535
BsrFI r'CCGG_y
Cuts at: 289 434 3010 3191 289
Size: 145 2576 181 2110
Fragments arranged by size:
2576 2110 181 145
BssSI C'ACGA_G
Cuts at: 3283 3991 3283
Size: 708 4304
BstAPI GCAn_nnn'nTGC
Cuts at: 2389 2389
Size: 5012
BstBI TT'CG_AA
Cuts at: 3373 3373
Size: 5012
BstYI r'GATC_y
Cuts at: 1 231 2037 2504 2527 2862 3108 3414 3426
Size: 230 1806 467 23 335 246 306 12
Cuts at: 3426 3512 4564 1
Size: 86 1052 449
Fragments arranged by size:
1806 1052 467 449 335 306 246 230
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 875 1574 875
Size: 699 4313
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2962 3143 3306 2962
Size: 181 163 4668
Fragments arranged by size:
4668 181 163
BtsI GCAGTG_nn'
Cuts at: 2255 2255
Size: 5012
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 907 942 907
Size: 35 4977
DraI TTT'AAA
Cuts at: 2011 2011
Size: 5012
DraIII CAC_nnn'GTG
Cuts at: 205 205
Size: 5012
DrdI GACnn_nn'nnGTC
Cuts at: 2718 4062 2718
Size: 1344 3668
EaeI y'GGCC_r
Cuts at: 428 2597 2771 3162 3189 4534 428
Size: 2169 174 391 27 1345 906
Fragments arranged by size:
2169 1345 906 391 174 27
EagI C'GGCC_G
Cuts at: 2597 2597
Size: 5012
EarI CTCTTCn'nnn_
Cuts at: 261 506 1112 1502 1787 3035 3245 261
Size: 245 606 390 285 1248 210 2028
Fragments arranged by size:
2028 1248 606 390 285 245 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3220 3962 4108 4822 3220
Size: 742 146 714 3410
Fragments arranged by size:
3410 742 714 146
EcoICRI GAG'CTC
Cuts at: 531 549 2024 531
Size: 18 1475 3519
Fragments arranged by size:
3519 1475 18
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 640 878 907 1136 1370 1820 2837 3269 3622
Size: 238 29 229 234 450 1017 432 353
Cuts at: 3622 4284 640
Size: 662 1368
Fragments arranged by size:
1368 1017 662 450 432 353 238 234
229 29
EcoO109I rG'GnC_Cy
Cuts at: 154 660 702 2151 154
Size: 506 42 1449 3015
Fragments arranged by size:
3015 1449 506 42
EcoRI G'AATT_C
Cuts at: 2016 2016
Size: 5012
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 535 3031 535
Size: 2496 2516
FspI TGC'GCA
Cuts at: 2793 2793
Size: 5012
HaeII r_GCGC'y
Cuts at: 2446 2694 3924 4695 2446
Size: 248 1230 771 2763
Fragments arranged by size:
2763 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 689 721 991 1023 1429 1461 2854 2884 2886
Size: 32 270 32 406 32 1393 30 2
Cuts at: 2886 2916 689
Size: 30 2785
Fragments arranged by size:
2785 1393 406 270 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 984 2051 984
Size: 1067 3945
HindIII A'AGCT_T
Cuts at: 517 517
Size: 5012
KasI G'GCGC_C
Cuts at: 2442 2690 2442
Size: 248 4764
KpnI G_GTAC'C
Cuts at: 2032 2032
Size: 5012
MluI A'CGCG_T
Cuts at: 4445 4445
Size: 5012
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 259 959 1004 3771 3955 259
Size: 700 45 2767 184 1316
Fragments arranged by size:
2767 1316 700 184 45
MscI TGG'CCA
Cuts at: 430 2773 430
Size: 2343 2669
MslI CAynn'nnrTG
Cuts at: 926 1271 3001 3128 926
Size: 345 1730 127 2810
Fragments arranged by size:
2810 1730 345 127
MspA1I CmG'CkG
Cuts at: 357 717 2123 2437 2797 3581 3826 357
Size: 360 1406 314 360 784 245 1543
Fragments arranged by size:
1543 1406 784 360 360 314 245
NaeI GCC'GGC
Cuts at: 291 3193 291
Size: 2902 2110
NarI GG'CG_CC
Cuts at: 2443 2691 2443
Size: 248 4764
NgoMIV G'CCGG_C
Cuts at: 289 3191 289
Size: 2902 2110
NsiI A_TGCA'T
Cuts at: 1039 1039
Size: 5012
NspI r_CATG'y
Cuts at: 1185 1864 2065 2565 4168 1185
Size: 679 201 500 1603 2029
Fragments arranged by size:
2029 1603 679 500 201
PasI CC'CwG_GG
Cuts at: 693 839 693
Size: 146 4866
PciI A'CATG_T
Cuts at: 1181 4164 1181
Size: 2983 2029
PmeI GTTT'AAAC
Cuts at: 2011 2011
Size: 5012
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3458 3490 3458
Size: 32 4980
PpuMI rG'GwC_Cy
Cuts at: 702 702
Size: 5012
PstI C_TGCA'G
Cuts at: 1109 2059 2744 1109
Size: 950 685 3377
Fragments arranged by size:
3377 950 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5012
PvuII CAG'CTG
Cuts at: 357 717 2437 2797 357
Size: 360 1720 360 2572
Fragments arranged by size:
2572 1720 360 360
RsrII CG'GwC_CG
Cuts at: 3207 3207
Size: 5012
SacI G_AGCT'C
Cuts at: 533 551 2026 533
Size: 18 1475 3519
Fragments arranged by size:
3519 1475 18
SalI G'TCGA_C
Cuts at: 982 2049 982
Size: 1067 3945
SapI GCTCTTCn'nnn_
Cuts at: 1787 3035 3245 1787
Size: 1248 210 3554
Fragments arranged by size:
3554 1248 210
SbfI CC_TGCA'GG
Cuts at: 2059 2059
Size: 5012
ScaI AGT'ACT
Cuts at: 4529 4529
Size: 5012
SfcI C'TryA_G
Cuts at: 33 1105 1428 2055 2740 3708 3899 33
Size: 1072 323 627 685 968 191 1146
Fragments arranged by size:
1146 1072 968 685 627 323 191
SfoI GGC'GCC
Cuts at: 2444 2692 2444
Size: 248 4764
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5012
SmaI CCC'GGG
Cuts at: 2034 2034
Size: 5012
SmlI C'TyrA_G
Cuts at: 410 2167 3082 3796 4058 410
Size: 1757 915 714 262 1364
Fragments arranged by size:
1757 1364 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5012
SphI G_CATG'C
Cuts at: 2065 2565 2065
Size: 500 4512
StyI C'CwwG_G
Cuts at: 295 1464 1521 2146 2500 295
Size: 1169 57 625 354 2807
Fragments arranged by size:
2807 1169 625 354 57
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2595 2728 3187 3396 2595
Size: 133 459 209 4211
Fragments arranged by size:
4211 459 209 133
TatI w'GTAC_w
Cuts at: 819 1877 4527 819
Size: 1058 2650 1304
Fragments arranged by size:
2650 1304 1058
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4529 4561 4529
Size: 32 4980
Tth111I GACn'n_nGTC
Cuts at: 979 2809 979
Size: 1830 3182
XbaI T'CTAG_A
Cuts at: 327 2043 327
Size: 1716 3296
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2507 2507
Size: 5012
XmaI C'CCGG_G
Cuts at: 2032 2032
Size: 5012
ZraI GAC'GTC
Cuts at: 1914 1914
Size: 5012
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AleI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I
BtgZI BtsI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NsiI NspI
PasI PciI PmeI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AgeI AhdI AloI ApaI AscI
AvrII BaeI BglI BmtI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I BtgI ClaI EcoNI EcoRV
FseI FspAI HpaI MfeI NcoI NdeI NheI NotI
NruI PacI PflMI PfoI PmlI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI