Restriction map for
pF1KB8681
(Circular) (Six-base) MAPSORT of: pF1KB8681.seq Check: 1670 from: 1 to: 4244
pF1KB8681 4244 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 353 353
Size: 4244
AccI GT'mk_AC
Cuts at: 1282 1282
Size: 4244
Acc65I G'GTAC_C
Cuts at: 1193 1260 1193
Size: 67 4177
AclI AA'CG_TT
Cuts at: 4084 4084
Size: 4244
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2069 2501 2854 3516 2069
Size: 432 353 662 2797
Fragments arranged by size:
2797 662 432 353
AfeI AGC'GCT
Cuts at: 373 373
Size: 4244
AflIII A'CryG_T
Cuts at: 433 1596 3396 3677 433
Size: 1163 1800 281 1000
Fragments arranged by size:
1800 1163 1000 281
AlwNI CAG_nnn'CTG
Cuts at: 192 651 1549 2987 192
Size: 459 898 1438 1449
Fragments arranged by size:
1449 1438 898 459
ApaLI G'TGCA_C
Cuts at: 835 3082 835
Size: 2247 1997
ApoI r'AATT_y
Cuts at: 1248 3597 3853 1248
Size: 2349 256 1639
Fragments arranged by size:
2349 1639 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4244
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4244
AvaI C'yCGr_G
Cuts at: 240 1264 240
Size: 1024 3220
BamHI G'GATC_C
Cuts at: 1269 1269
Size: 4244
BanI G'GyrC_C
Cuts at: 341 549 931 995 1193 1260 1674 1922 1957
Size: 208 382 64 198 67 414 248 35
Cuts at: 1957 341
Size: 2628
Fragments arranged by size:
2628 414 382 248 208 198 67 64
35
BanII G_rGCy'C
Cuts at: 247 274 315 1258 2288 247
Size: 27 41 943 1030 2203
Fragments arranged by size:
2203 1030 943 41 27
BbeI G_GCGC'C
Cuts at: 1678 1926 1678
Size: 248 3996
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 457 491 457
Size: 34 4210
BciVI GTATCCnnnnn_n'
Cuts at: 2142 3198 4244 2142
Size: 1056 1046 2142
Fragments arranged by size:
2142 1056 1046
BclI T'GATC_A
Cuts at: 1764 1764
Size: 4244
BfrBI ATG'CAT
Cuts at: 1153 1153
Size: 4244
BglI GCCn_nnn'nGGC
Cuts at: 215 685 215
Size: 470 3774
BglII A'GATC_T
Cuts at: 1759 3796 1759
Size: 2037 2207
BlpI GC'TnA_GC
Cuts at: 375 1356 375
Size: 981 3263
Bme1580I G_kGCm'C
Cuts at: 554 839 1869 1962 3086 554
Size: 285 1030 93 1124 1712
Fragments arranged by size:
1712 1124 1030 285 93
BmgBI CAC'GTC
Cuts at: 434 434
Size: 4244
BmrI ACTGGGnnnn_n'
Cuts at: 1456 1537 1872 4016 1456
Size: 81 335 2144 1684
Fragments arranged by size:
2144 1684 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 481 2611 481
Size: 2130 2114
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 186 988 186
Size: 802 3442
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 607 970 1420 2299 3013 3311 607
Size: 363 450 879 714 298 1540
Fragments arranged by size:
1540 879 714 450 363 298
BsaI GGTCTCn'nnnn_
Cuts at: 3948 3948
Size: 4244
BsaAI yAC'GTr
Cuts at: 1597 1597
Size: 4244
BsaBI GATnn'nnATC
Cuts at: 6 1758 3795 6
Size: 1752 2037 455
Fragments arranged by size:
2037 1752 455
BsaHI Gr'CG_yC
Cuts at: 883 1675 1923 2625 4121 883
Size: 792 248 702 1496 1006
Fragments arranged by size:
1496 1006 792 702 248
BsaWI w'CCGG_w
Cuts at: 1433 1954 2632 3043 3190 3464 1433
Size: 521 678 411 147 274 2213
Fragments arranged by size:
2213 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 790 820 829 859 790
Size: 30 9 30 4175
Fragments arranged by size:
4175 30 30 9
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 525 591 811 525
Size: 66 220 3958
Fragments arranged by size:
3958 220 66
BseYI C'CCAG_C
Cuts at: 171 185 282 675 761 1230 1669 2427 3092
Size: 14 97 393 86 469 439 758 665
Cuts at: 3092 3500 171
Size: 408 915
Fragments arranged by size:
915 758 665 469 439 408 393 97
86 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 974 974
Size: 4244
BsiEI CG_ry'CG
Cuts at: 104 1786 1832 3062 104
Size: 1682 46 1230 1286
Fragments arranged by size:
1682 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 839 1258 2036 2226 3086 839
Size: 419 778 190 860 1997
Fragments arranged by size:
1997 860 778 419 190
BsmI GAATG_Cn'
Cuts at: 1153 1153
Size: 4244
Bsp1286I G_dGCh'C
Cuts at: 247 274 315 554 839 1258 1869 1962 2036
Size: 27 41 239 285 419 611 93 74
Cuts at: 2036 2226 2288 3086 247
Size: 190 62 798 1405
Fragments arranged by size:
1405 798 611 419 285 239 190 93
74 62 41 27
BspHI T'CATG_A
Cuts at: 2676 2676
Size: 4244
BspMI ACCTGCnnnn'nnnn_
Cuts at: 353 1205 1294 1508 1810 2191 353
Size: 852 89 214 302 381 2406
Fragments arranged by size:
2406 852 381 302 214 89
BsrBI CCG'CTC
Cuts at: 50 2536 2590 4074 4239 50
Size: 2486 54 1484 165 55
Fragments arranged by size:
2486 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2156 2156
Size: 4244
BsrFI r'CCGG_y
Cuts at: 1070 2242 2423 1070
Size: 1172 181 2891
Fragments arranged by size:
2891 1172 181
BssHII G'CGCG_C
Cuts at: 348 348
Size: 4244
BssSI C'ACGA_G
Cuts at: 2515 3223 2515
Size: 708 3536
BstAPI GCAn_nnn'nTGC
Cuts at: 1621 1621
Size: 4244
BstBI TT'CG_AA
Cuts at: 2605 2605
Size: 4244
BstEII G'GTnAC_C
Cuts at: 711 711
Size: 4244
BstXI CCAn_nnnn'nTGG
Cuts at: 561 948 561
Size: 387 3857
BstYI r'GATC_y
Cuts at: 1 1269 1736 1759 2094 2340 2646 2658 2744
Size: 1268 467 23 335 246 306 12 86
Cuts at: 2744 3796 1
Size: 1052 449
Fragments arranged by size:
1268 1052 467 449 335 306 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 248 274 923 935 107
Size: 141 26 649 12 3416
Fragments arranged by size:
3416 649 141 26 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2194 2375 2538 2194
Size: 181 163 3900
Fragments arranged by size:
3900 181 163
BtsI GCAGTG_nn'
Cuts at: 820 1487 820
Size: 667 3577
DraI TTT'AAA
Cuts at: 1243 1243
Size: 4244
DrdI GACnn_nn'nnGTC
Cuts at: 604 898 1950 3294 604
Size: 294 1052 1344 1554
Fragments arranged by size:
1554 1344 1052 294
EaeI y'GGCC_r
Cuts at: 938 959 1829 2003 2394 2421 3766 938
Size: 21 870 174 391 27 1345 1416
Fragments arranged by size:
1416 1345 870 391 174 27 21
EagI C'GGCC_G
Cuts at: 1829 1829
Size: 4244
EarI CTCTTCn'nnn_
Cuts at: 650 2267 2477 650
Size: 1617 210 2417
Fragments arranged by size:
2417 1617 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 835 2452 3194 3340 4054 835
Size: 1617 742 146 714 1025
Fragments arranged by size:
1617 1025 742 714 146
EcoICRI GAG'CTC
Cuts at: 1256 1256
Size: 4244
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 186 988 2069 2501 2854 3516 186
Size: 802 1081 432 353 662 914
Fragments arranged by size:
1081 914 802 662 432 353
EcoNI CCTnn'n_nnAGG
Cuts at: 1030 1030
Size: 4244
EcoO109I rG'GnC_Cy
Cuts at: 357 1023 1383 357
Size: 666 360 3218
Fragments arranged by size:
3218 666 360
EcoRI G'AATT_C
Cuts at: 1248 1248
Size: 4244
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2263 2263
Size: 4244
FspI TGC'GCA
Cuts at: 514 2025 514
Size: 1511 2733
HaeII r_GCGC'y
Cuts at: 139 298 375 620 1678 1926 3156 3927 139
Size: 159 77 245 1058 248 1230 771 456
Fragments arranged by size:
1230 1058 771 456 248 245 159 77
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2086 2116 2118 2148 2086
Size: 30 2 30 4182
Fragments arranged by size:
4182 30 30 2
HincII GTy'rAC
Cuts at: 1283 1283
Size: 4244
KasI G'GCGC_C
Cuts at: 1674 1922 1674
Size: 248 3996
KpnI G_GTAC'C
Cuts at: 1197 1264 1197
Size: 67 4177
MluI A'CGCG_T
Cuts at: 3677 3677
Size: 4244
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3003 3187 3003
Size: 184 4060
MscI TGG'CCA
Cuts at: 940 2005 940
Size: 1065 3179
MslI CAynn'nnrTG
Cuts at: 559 2233 2360 559
Size: 1674 127 2443
Fragments arranged by size:
2443 1674 127
MspA1I CmG'CkG
Cuts at: 192 276 411 958 1355 1669 2029 2813 3058
Size: 84 135 547 397 314 360 784 245
Cuts at: 3058 192
Size: 1378
Fragments arranged by size:
1378 784 547 397 360 314 245 135
84
NaeI GCC'GGC
Cuts at: 2425 2425
Size: 4244
NarI GG'CG_CC
Cuts at: 1675 1923 1675
Size: 248 3996
NcoI C'CATG_G
Cuts at: 107 107
Size: 4244
NgoMIV G'CCGG_C
Cuts at: 2423 2423
Size: 4244
NsiI A_TGCA'T
Cuts at: 1155 1155
Size: 4244
NspI r_CATG'y
Cuts at: 1297 1797 3400 1297
Size: 500 1603 2141
Fragments arranged by size:
2141 1603 500
PasI CC'CwG_GG
Cuts at: 303 315 303
Size: 12 4232
PciI A'CATG_T
Cuts at: 3396 3396
Size: 4244
PflMI CCAn_nnn'nTGG
Cuts at: 495 495
Size: 4244
PfoI T'CCnGG_A
Cuts at: 1004 1004
Size: 4244
PmeI GTTT'AAAC
Cuts at: 1243 1243
Size: 4244
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 514 546 898 930 2690 2722 514
Size: 32 352 32 1760 32 2036
Fragments arranged by size:
2036 1760 352 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 357 1023 357
Size: 666 3578
PstI C_TGCA'G
Cuts at: 258 410 448 1291 1976 258
Size: 152 38 843 685 2526
Fragments arranged by size:
2526 843 685 152 38
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4244
PvuII CAG'CTG
Cuts at: 411 1669 2029 411
Size: 1258 360 2626
Fragments arranged by size:
2626 1258 360
RsrII CG'GwC_CG
Cuts at: 2439 2439
Size: 4244
SacI G_AGCT'C
Cuts at: 1258 1258
Size: 4244
SacII CC_GC'GG
Cuts at: 277 277
Size: 4244
SalI G'TCGA_C
Cuts at: 1281 1281
Size: 4244
SapI GCTCTTCn'nnn_
Cuts at: 2267 2477 2267
Size: 210 4034
SbfI CC_TGCA'GG
Cuts at: 1291 1291
Size: 4244
ScaI AGT'ACT
Cuts at: 733 3761 733
Size: 3028 1216
SexAI A'CCwGG_T
Cuts at: 488 632 488
Size: 144 4100
SfcI C'TryA_G
Cuts at: 33 254 406 444 540 1287 1972 2940 3131
Size: 221 152 38 96 747 685 968 191
Cuts at: 3131 33
Size: 1146
Fragments arranged by size:
1146 968 747 685 221 191 152 96
38
SfoI GGC'GCC
Cuts at: 1676 1924 1676
Size: 248 3996
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4244
SmaI CCC'GGG
Cuts at: 1266 1266
Size: 4244
SmlI C'TyrA_G
Cuts at: 622 985 1399 2314 3028 3290 622
Size: 363 414 915 714 262 1576
Fragments arranged by size:
1576 915 714 414 363 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4244
SphI G_CATG'C
Cuts at: 1297 1797 1297
Size: 500 3744
StuI AGG'CCT
Cuts at: 1225 1225
Size: 4244
StyI C'CwwG_G
Cuts at: 107 158 1378 1732 107
Size: 51 1220 354 2619
Fragments arranged by size:
2619 1220 354 51
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 140 730 940 1038 1827 1960 2419 2628 140
Size: 590 210 98 789 133 459 209 1756
Fragments arranged by size:
1756 789 590 459 210 209 133 98
TatI w'GTAC_w
Cuts at: 425 731 944 3759 425
Size: 306 213 2815 910
Fragments arranged by size:
2815 910 306 213
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 790 822 829 861 3761 3793 790
Size: 32 7 32 2900 32 1241
Fragments arranged by size:
2900 1241 32 32 32 7
Tth111I GACn'n_nGTC
Cuts at: 2041 2041
Size: 4244
XbaI T'CTAG_A
Cuts at: 1275 1275
Size: 4244
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 769 876 1739 769
Size: 107 863 3274
Fragments arranged by size:
3274 863 107
XmaI C'CCGG_G
Cuts at: 1264 1264
Size: 4244
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AatII AflII AgeI AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BmtI Bpu10I BsiWI
BsmBI BspEI BsrGI BstZ17I Bsu36I ClaI CspCI DraIII
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI PacI PmlI PshAI PsiI PspOMI PspXI
PsrI SanDI SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI XmnI ZraI