Restriction map for
pF1KB8683
(Circular) (Six-base) MAPSORT of: pF1KB8683.seq Check: 4311 from: 1 to: 5129
pF1KB8683 5129 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AccI GT'mk_AC
Cuts at: 2167 2167
Size: 5129
Acc65I G'GTAC_C
Cuts at: 1041 2145 1041
Size: 1104 4025
AclI AA'CG_TT
Cuts at: 4969 4969
Size: 5129
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 198 217 301 328 973 998 1030 2954 3386
Size: 19 84 27 645 25 32 1924 432
Cuts at: 3386 3739 4401 198
Size: 353 662 926
Fragments arranged by size:
1924 926 662 645 432 353 84 32
27 25 19
AflII C'TTAA_G
Cuts at: 549 549
Size: 5129
AflIII A'CryG_T
Cuts at: 1250 2481 4281 4562 1250
Size: 1231 1800 281 1817
Fragments arranged by size:
1817 1800 1231 281
AhdI GACnn_n'nnGTC
Cuts at: 1467 1467
Size: 5129
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1436 1436
Size: 5129
AlwNI CAG_nnn'CTG
Cuts at: 678 2434 3872 678
Size: 1756 1438 1935
Fragments arranged by size:
1935 1756 1438
ApaLI G'TGCA_C
Cuts at: 3967 3967
Size: 5129
ApoI r'AATT_y
Cuts at: 190 451 1231 1937 1984 2133 4482 4738 190
Size: 261 780 706 47 149 2349 256 581
Fragments arranged by size:
2349 780 706 581 261 256 149 47
AseI AT'TA_AT
Cuts at: 20 20
Size: 5129
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5129
AvaI C'yCGr_G
Cuts at: 1702 2149 1702
Size: 447 4682
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1557 1590 1557
Size: 33 5096
BamHI G'GATC_C
Cuts at: 2154 2154
Size: 5129
BanI G'GyrC_C
Cuts at: 745 1041 2145 2559 2807 2842 745
Size: 296 1104 414 248 35 3032
Fragments arranged by size:
3032 1104 414 296 248 35
BanII G_rGCy'C
Cuts at: 632 689 1194 1703 1792 2143 3173 632
Size: 57 505 509 89 351 1030 2588
Fragments arranged by size:
2588 1030 509 505 351 89 57
BbeI G_GCGC'C
Cuts at: 2563 2811 2563
Size: 248 4881
BbsI GAAGACnn'nnnn_
Cuts at: 667 737 979 1300 667
Size: 70 242 321 4496
Fragments arranged by size:
4496 321 242 70
BbvCI CC'TCA_GC
Cuts at: 317 436 590 317
Size: 119 154 4856
Fragments arranged by size:
4856 154 119
BciVI GTATCCnnnnn_n'
Cuts at: 798 3027 4083 5129 798
Size: 2229 1056 1046 798
Fragments arranged by size:
2229 1056 1046 798
BclI T'GATC_A
Cuts at: 737 2649 737
Size: 1912 3217
BglI GCCn_nnn'nGGC
Cuts at: 1351 1351
Size: 5129
BglII A'GATC_T
Cuts at: 2644 4681 2644
Size: 2037 3092
BlpI GC'TnA_GC
Cuts at: 2241 2241
Size: 5129
Bme1580I G_kGCm'C
Cuts at: 2754 2847 3971 2754
Size: 93 1124 3912
Fragments arranged by size:
3912 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 2062 2341 2422 2757 4901 2062
Size: 279 81 335 2144 2290
Fragments arranged by size:
2290 2144 335 279 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3496 3496
Size: 5129
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 313 544 843 961 1168 1309 1519 313
Size: 231 299 118 207 141 210 3923
Fragments arranged by size:
3923 299 231 210 207 141 118
Bpu10I CC'TnA_GC
Cuts at: 317 436 590 317
Size: 119 154 4856
Fragments arranged by size:
4856 154 119
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 634 916 2305 3184 3898 4196 634
Size: 282 1389 879 714 298 1567
Fragments arranged by size:
1567 1389 879 714 298 282
BsaI GGTCTCn'nnnn_
Cuts at: 121 4833 121
Size: 4712 417
BsaAI yAC'GTr
Cuts at: 693 1253 1605 2482 693
Size: 560 352 877 3340
Fragments arranged by size:
3340 877 560 352
BsaBI GATnn'nnATC
Cuts at: 6 2643 4680 6
Size: 2637 2037 455
Fragments arranged by size:
2637 2037 455
BsaHI Gr'CG_yC
Cuts at: 2560 2808 3510 5006 2560
Size: 248 702 1496 2683
Fragments arranged by size:
2683 1496 702 248
BsaWI w'CCGG_w
Cuts at: 860 2318 2839 3517 3928 4075 4349 860
Size: 1458 521 678 411 147 274 1640
Fragments arranged by size:
1640 1458 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1405 1698 1405
Size: 293 4836
BseYI C'CCAG_C
Cuts at: 339 578 1104 1376 1791 2554 3312 3977 4385
Size: 239 526 272 415 763 758 665 408
Cuts at: 4385 339
Size: 1083
Fragments arranged by size:
1083 763 758 665 526 415 408 272
239
BsiEI CG_ry'CG
Cuts at: 104 1320 2671 2717 3947 104
Size: 1216 1351 46 1230 1286
Fragments arranged by size:
1351 1286 1230 1216 46
BsiHKAI G_wGCw'C
Cuts at: 689 2143 2921 3111 3971 689
Size: 1454 778 190 860 1847
Fragments arranged by size:
1847 1454 860 778 190
BsmI GAATG_Cn'
Cuts at: 464 1095 1425 464
Size: 631 330 4168
Fragments arranged by size:
4168 631 330
Bsp1286I G_dGCh'C
Cuts at: 632 689 1194 1703 1792 2143 2754 2847 2921
Size: 57 505 509 89 351 611 93 74
Cuts at: 2921 3111 3173 3971 632
Size: 190 62 798 1790
Fragments arranged by size:
1790 798 611 509 505 351 190 93
89 74 62 57
BspHI T'CATG_A
Cuts at: 782 3561 782
Size: 2779 2350
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2179 2393 2695 3076 2179
Size: 214 302 381 4232
Fragments arranged by size:
4232 381 302 214
BsrBI CCG'CTC
Cuts at: 50 171 855 3421 3475 4959 5124 50
Size: 121 684 2566 54 1484 165 55
Fragments arranged by size:
2566 1484 684 165 121 55 54
BsrDI GCAATG_nn'
Cuts at: 3041 3041
Size: 5129
BsrFI r'CCGG_y
Cuts at: 3127 3308 3127
Size: 181 4948
BsrGI T'GTAC_A
Cuts at: 380 380
Size: 5129
BssSI C'ACGA_G
Cuts at: 1309 3400 4108 1309
Size: 2091 708 2330
Fragments arranged by size:
2330 2091 708
BstAPI GCAn_nnn'nTGC
Cuts at: 2506 2506
Size: 5129
BstBI TT'CG_AA
Cuts at: 3490 3490
Size: 5129
BstXI CCAn_nnnn'nTGG
Cuts at: 522 1363 1431 1605 522
Size: 841 68 174 4046
Fragments arranged by size:
4046 841 174 68
BstYI r'GATC_y
Cuts at: 1 301 1265 2154 2621 2644 2979 3225 3531
Size: 300 964 889 467 23 335 246 306
Cuts at: 3531 3543 3629 4681 1
Size: 12 86 1052 449
Fragments arranged by size:
1052 964 889 467 449 335 306 300
246 86 23 12
BtgI C'CryG_G
Cuts at: 386 386
Size: 5129
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3079 3260 3423 3079
Size: 181 163 4785
Fragments arranged by size:
4785 181 163
BtsI GCAGTG_nn'
Cuts at: 978 1387 1726 2372 978
Size: 409 339 646 3735
Fragments arranged by size:
3735 646 409 339
DraI TTT'AAA
Cuts at: 2128 2128
Size: 5129
DrdI GACnn_nn'nnGTC
Cuts at: 2835 4179 2835
Size: 1344 3785
EaeI y'GGCC_r
Cuts at: 158 1317 2714 2888 3279 3306 4651 158
Size: 1159 1397 174 391 27 1345 636
Fragments arranged by size:
1397 1345 1159 636 391 174 27
EagI C'GGCC_G
Cuts at: 1317 2714 1317
Size: 1397 3732
EarI CTCTTCn'nnn_
Cuts at: 1031 1917 2031 3152 3362 1031
Size: 886 114 1121 210 2798
Fragments arranged by size:
2798 1121 886 210 114
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 658 3337 4079 4225 4939 658
Size: 2679 742 146 714 848
Fragments arranged by size:
2679 848 742 714 146
EcoICRI GAG'CTC
Cuts at: 687 2141 687
Size: 1454 3675
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 198 217 301 313 328 544 843 961 973
Size: 19 84 12 15 216 299 118 12
Cuts at: 973 998 1030 1168 1309 1519 2954 3386 3739
Size: 25 32 138 141 210 1435 432 353
Cuts at: 3739 4401 198
Size: 662 926
Fragments arranged by size:
1435 926 662 432 353 299 216 210
141 138 118 84 32 25 19 15
12 12
EcoO109I rG'GnC_Cy
Cuts at: 537 774 1143 1494 1857 2268 537
Size: 237 369 351 363 411 3398
Fragments arranged by size:
3398 411 369 363 351 237
EcoRI G'AATT_C
Cuts at: 451 1984 2133 451
Size: 1533 149 3447
Fragments arranged by size:
3447 1533 149
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 527 1003 3148 527
Size: 476 2145 2508
Fragments arranged by size:
2508 2145 476
FspI TGC'GCA
Cuts at: 2910 2910
Size: 5129
HaeII r_GCGC'y
Cuts at: 2563 2811 4041 4812 2563
Size: 248 1230 771 2880
Fragments arranged by size:
2880 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 429 461 1168 1200 1453 1485 2971 3001 3003
Size: 32 707 32 253 32 1486 30 2
Cuts at: 3003 3033 429
Size: 30 2525
Fragments arranged by size:
2525 1486 707 253 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2168 2168
Size: 5129
HindIII A'AGCT_T
Cuts at: 1510 1637 1510
Size: 127 5002
KasI G'GCGC_C
Cuts at: 2559 2807 2559
Size: 248 4881
KpnI G_GTAC'C
Cuts at: 1045 2149 1045
Size: 1104 4025
MluI A'CGCG_T
Cuts at: 4562 4562
Size: 5129
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 92 1044 3888 4072 92
Size: 952 2844 184 1149
Fragments arranged by size:
2844 1149 952 184
MscI TGG'CCA
Cuts at: 2890 2890
Size: 5129
MslI CAynn'nnrTG
Cuts at: 952 3118 3245 952
Size: 2166 127 2836
Fragments arranged by size:
2836 2166 127
MspA1I CmG'CkG
Cuts at: 1322 2240 2554 2914 3698 3943 1322
Size: 918 314 360 784 245 2508
Fragments arranged by size:
2508 918 784 360 314 245
NaeI GCC'GGC
Cuts at: 3310 3310
Size: 5129
NarI GG'CG_CC
Cuts at: 2560 2808 2560
Size: 248 4881
NcoI C'CATG_G
Cuts at: 386 386
Size: 5129
NgoMIV G'CCGG_C
Cuts at: 3308 3308
Size: 5129
NspI r_CATG'y
Cuts at: 2182 2682 4285 2182
Size: 500 1603 3026
Fragments arranged by size:
3026 1603 500
PciI A'CATG_T
Cuts at: 4281 4281
Size: 5129
PflMI CCAn_nnn'nTGG
Cuts at: 678 678
Size: 5129
PmeI GTTT'AAAC
Cuts at: 2128 2128
Size: 5129
PmlI CAC'GTG
Cuts at: 1253 1253
Size: 5129
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3575 3607 3575
Size: 32 5097
PpuMI rG'GwC_Cy
Cuts at: 537 774 1143 537
Size: 237 369 4523
Fragments arranged by size:
4523 369 237
PstI C_TGCA'G
Cuts at: 1130 1787 2176 2861 1130
Size: 657 389 685 3398
Fragments arranged by size:
3398 685 657 389
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5129
PvuII CAG'CTG
Cuts at: 2554 2914 2554
Size: 360 4769
RsrII CG'GwC_CG
Cuts at: 3324 3324
Size: 5129
SacI G_AGCT'C
Cuts at: 689 2143 689
Size: 1454 3675
SalI G'TCGA_C
Cuts at: 2166 2166
Size: 5129
SapI GCTCTTCn'nnn_
Cuts at: 3152 3362 3152
Size: 210 4919
SbfI CC_TGCA'GG
Cuts at: 2176 2176
Size: 5129
ScaI AGT'ACT
Cuts at: 4646 4646
Size: 5129
SfcI C'TryA_G
Cuts at: 33 1126 1783 2172 2857 3825 4016 33
Size: 1093 657 389 685 968 191 1146
Fragments arranged by size:
1146 1093 968 685 657 389 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 1351 1351
Size: 5129
SfoI GGC'GCC
Cuts at: 2561 2809 2561
Size: 248 4881
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5129
SmaI CCC'GGG
Cuts at: 2151 2151
Size: 5129
SmlI C'TyrA_G
Cuts at: 549 649 931 2284 3199 3913 4175 549
Size: 100 282 1353 915 714 262 1503
Fragments arranged by size:
1503 1353 915 714 282 262 100
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5129
SphI G_CATG'C
Cuts at: 2182 2682 2182
Size: 500 4629
StuI AGG'CCT
Cuts at: 441 643 1345 1950 441
Size: 202 702 605 3620
Fragments arranged by size:
3620 702 605 202
StyI C'CwwG_G
Cuts at: 386 407 620 1157 1553 1853 2263 2617 386
Size: 21 213 537 396 300 410 354 2898
Fragments arranged by size:
2898 537 410 396 354 300 213 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2712 2845 3304 3513 2712
Size: 133 459 209 4328
Fragments arranged by size:
4328 459 209 133
TatI w'GTAC_w
Cuts at: 380 4644 380
Size: 4264 865
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1393 1425 4646 4678 1393
Size: 32 3221 32 1844
Fragments arranged by size:
3221 1844 32 32
Tth111I GACn'n_nGTC
Cuts at: 2926 2926
Size: 5129
XbaI T'CTAG_A
Cuts at: 2160 2160
Size: 5129
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1431 1836 2624 1431
Size: 405 788 3936
Fragments arranged by size:
3936 788 405
XmaI C'CCGG_G
Cuts at: 2149 2149
Size: 5129
XmnI GAAnn'nnTTC
Cuts at: 507 507
Size: 5129
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AhdI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI
BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PflMI PmeI
PmlI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AgeI AleI AloI ApaI AscI
AvrII BcgI BfrBI BmgBI BmtI BsaXI BsgI BsiWI
BsmBI BspEI BssHII BstEII BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PfoI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SgrAI
SnaBI SrfI SspI SwaI XhoI ZraI